Yeast Cell Counting
Standardizing Yeast Fermentation
The concentration and viability of yeast cells are important factors in a variety of fermentation and production processes. The NucleoCounter® YC-100™ utilizes a unique imaging technology to determine the precise concentration and viability of yeast cells in a simple and fast 2-step protocol.
Yeast counting determines the amount of yeast in a given volume. It can also determine the amount of living versus dead yeast cells. Brewing has different requirements in terms of yeast counting which are described below. The NucleoCounter® YC-100™ determines the number of yeast cells and the percentage of living cells (the viability) and provides essential precision and reliability for brewers.
YC-100™ Key benefits:
- Easy operation
- 30 sec. analysis time
- No cleaning or calibration
- Maintenance and service free
- Excellent reproducibility
- Safe sample handling and disposal
- Excellent for aggregated cells
Applications of NucleoCounter® YC-100™ in Brewing
Brewing beer requires special care to achieve the same distinctive taste every time. To ensure consistency, one of the critical factors is the amount of live and dead yeast cells used when pitching. The NucleoCounter® YC-100™ provides the user with a fast, objective and high precision measurement of yeast count and viability. The unique NucleoCassette™ media for the YC-100™ ensures easy handling and eliminates human subjectivity, resulting in very high accuracy and precision.
The NucleoCounter® YC-100™ provides a quick and easy way to monitor yeast concentration while determining yeast count and viability. The analyzed volume is more than three times greater than the volume commonly analyzed when using a manual count. This makes the NucleoCounter® YC-100™ one of the leading instruments on the market for precision and accuracy.
To obtain the total number of yeast cells, a small sample is lysed with reagent Y100 and loaded into the NucleoCassette™. The immobilized propidium iodide inside the cassette is dissolved and stains the nuclei of all the cells. The cassette is then placed into the YC-100™ and run. To provide viability, a number of living cells is needed. For this, a sample without the lysis agent is loaded and run. As propidium iodide only stains dead cells, the counted cells will be dead and the number of living cells can be easily calculated.