Rapid, quality samples for regenerative medicine

Cell therapy at OrthoIdaho

Regenerative medicine seeks to develop the science and tools that can help repair or replace damaged and diseased human cells or tissues to restore normal function. It is revolutionizing treatment in the 21st century through the transplantation of stem cells, progenitor cells or tissue and the use of cells as delivery-vehicles for therapeutic agents such as genes and cytokines. All these advances lead to the stimulation of the body’s own repair processes.

By using different cell sub-populations extracted from blood or bone marrow, OrthoIdaho involves the patient’s own body to achieve long-lasting results and offers longer lasting respite and an alternative to surgery to its patients. Knees, joints, tendons and back discs are the most common treatment locations for this practice.


The key challenges are to be able to deliver rapid and accurate quality assurance to both patients and practitioners and to make continuous improvements to all the processes leading to better care.

For regenerative medicine, the latest studies on platelet-rich plasma and bone marrow aspirate and concentrate show that these treatments allow more directed healing to joints, tendons, muscles and discs. This is allowing patients a greater amount of time and activity before joint or disc replacements.

User commentary

“We purchased the NucleoCounter® NC-200™ because the nucleated cell count enables us to see the percentage of quality cells obtained in each bone marrow sample. A percentage of these are mesenchymal stem cells (MSCs) believed to be part of the regenerative or healing process.

There are no other cell counters on the market that allow us to do this with the ease of use of the NC-200™ and its technology transfers very well to the clinical setting. We found that, after our bone marrow sample is drawn, we can run a cell count and look at both total nucleated cell count and viability and relay this information to the patient, giving vital assurance that a quality sample has been taken. If not adequate, we have the option of a second aspiration. The NC-200™ also allows us, in the long run, to monitor the quality of the samples we are getting and adjust and improve our optimization technologies.

A scientist wearing safety glasses and gloves is preparing samples for cell counting. His colleague is setting up the assay parameters in the NucleoView™ software on her laptop.

This is a quick, clean system which is simple to use, and the data is easy to read. It is fast and easy to transfer the data into our patient database and print out for the patient making the NC-200™ an essential component within our treatment process.”

Amanda Dunahoo, Assistant to Dr. Anthony Joseph

Learn more about Ortholdaho (Acquired by Portneuf).