Nucleocounter® instruments offer robust and reliable solutions for counting cells in clumps and aggregates. They use fluorescent dyes to stain cell nuclei and employ a powerful algorithm to segment single nuclei within each smaller aggregate.
The NucleoCounter® instruments provide a robust and precise method to effectively determine viability and total cell count of MSCs derived from different sources e.g. lipoaspirates and bone marrow aspirates.
The NucleoView™ software for NucleoCounter® can be set into a restricted mode, so the user via the software itself cannot violate the 21 CFR Part 11 regulations.
We have employed an image based cytometer, NucleoCounter® NC-3000™, for quantifying different events in the apoptotic process. Compared to flow cytometric analyses (BD LSRII), NC-3000™ demonstrated accurate and precise determination of phosphatidylserine translocation, Caspase 3/7 activation and depolarization of the mitochondrial membrane.