NucleoCounter® NC-200™
Automated cell counter
One cassette. One setting. Truly objective
- Consistent cell counting and analysis
- GMP and 21 CFR Part 11 ready
- Used with Via1-Cassette™ to eliminate human error
- No calibration required
- Unlimited software licenses
The NucleoCounter® NC-200™ is a high precision automated cell counter utilizing state-of-the-art image cytometry. It counts suspension cells, aggregated cells, CAR-T cells, stem cells and cells on microcarriers.
With user-adaptable protocols and specialized assays, the NucleoCounter® NC-200™ provides an all-in-one solution for cell counting and cell viability determination.
The Via1-Cassette™ is used with this counter for automated staining and sample handling. The cassette is volume calibrated to ensure high precision and cell reproducibility.
Combining simplicity and innovation in automated cell counting
Eliminate human bias in cell counting – Via1-Cassette™
During manual cell count, the subjective evaluation of the definition of a cell introduces bias to the result. The automatic cell counter NC-200™ is designed to limit human interference in counting. With the Via1-Cassette™, all errors introduced during pipetting and staining are avoided. The Via1-Cassette™ includes an in-built pipette and the immobilized fluorescent dyes acridine orange and DAPI automatically stain the total and dead cell populations, respectively.
Read MoreCell count presentation and data management
The NucleoView™ software allows visual inspection of the fluorescence image and the opportunity to verify the cell count. Specific event populations can be selected in the scatter plots and examined visually to determine the validity of their inclusion or exclusion from the final cell counting results. The adapted protocol can be then saved for future measurements, increasing reproducibility.
Read MoreGMP Ready Automated Cell Counter
The data produced with the NucleoCounter® NC-200™ are traceable and saved within our intuitive NucleoView software™. The optional 21 CFR Part 11 license includes GMP ready features such as logging in with user-group credentials which documents actions in a secured audit trail format. The NucleoCounter® NC-200™ is easy to clean.
Read More
A safe, non-toxic cell counting system
A closed cell counting system that uses self-contained disposable cassettes, helps the user stay protected when handling samples. No pipettes, no exposure to (toxic) reagents, and minimal clean-up make the NucleoCounter® system a safe option.
Automated Cell Counter NucleoCounter® NC-200™
Counting cells in clumps with NucleoCounter® NC-200™
With the Aggregated Cells Assay, the NucleoCounter® NC-200™ can count cells even from a sample of highly aggregated cells or cells in clumps.
Read MoreTotal cell count and viability of microcarrier cultures
The NucleoCounter® NC-200™ offers a unique protocol to determine the total cell count and viability of cell cultures grown on microcarriers.
Read MoreCounting of mesenchymal stem cells in regenerative medicine
With the NucleoCounter® NC-200™, it is easy to precisely determine viability and cell count of MSC’s derived from different sources e.g. lipoaspirates and bone marrow aspirates.
Read MoreCounting of peripheral blood mononucleated cells in whole blood samples
With the automatic cell counter NC-200™, the total viability and cell count of leukocytes can easily be determined in cell samples containing both platelets and red blood cells.
Read More21 CFR Part 11 Ready
The NucleoCounter® NC-200™ is designed to be easily implemented in 21 CFR Part 11 compliant laboratories. Key features include control of user access and data export, and secure audit trail.
Read MoreMonitoring the manufacturing of CAR-T cells
The NucleoCounter® NC-200™ provides robust and accurate determination of cell count and viability of leukocytes and T cells during adoptive CAR-T cell manufacturing.
Read More| Technical Specifications | |
|---|---|
| Product number | 900-0201 |
| Loading volume | App. 60 μl loaded into the Via1-Cassette™ |
| Measurement volume | 1.4 μl (volume-calibrated Via1-Cassette™) |
| Analysis time | 50 seconds (one step), 120 seconds (two step) |
| Optimal range | 5×104 to 5×106 cells/ml |
| Size | 38 × 26 × 22 cm (W × H × D) |
| Weight | 3 kg / 6.7 lb |
| Software | NucleoView™ software |
| Application Notes | ||
|---|---|---|
| No. | Assay Name | Download |
| 994-0220 | Viability and Cell Count – Mammalian Cells | Open PDF |
| 994-0221 | Viability and Cell Count – Microcarrier Cultured Cells | Open PDF |
| 994-0203 | Viability and Cell Count – Aggregated Mammalian Cells | Open PDF |
| 994-0207 | Viability and Cell Count – Whole Blood Assay | Open PDF |
| 994-0214 | Viability and Cell Count – with Reagent A100 and B | Open PDF |
| 994-0209 | 994-0209 Tech Note PQ NC-200 | Open PDF |
| 994-0210 | 994-0210 Tech Note PQ NC-200 (checklist) | Open PDF |
| Documentation | ||
|---|---|---|
| No. | Document Name | Download |
| 994-0218 | Performance of NucleoCounter® NC-200™ | Open PDF |
| 994-0216 | NucleoCounter® NC-200™ – Variation and Statistics | Open PDF |
| 994-0208 | White Paper 21 CFR Part 11 for NC-200™ Software | Open PDF |
| 994-0031 | LED Life Time Considerations for the NucleoCounter® NC-200™ | Open PDF |
| / | Certificate of Conformity | Open PDF |
| Consumables | Accessories |
|---|---|
| Via1-Cassette™, 1 Box (100 pcs.) | Laptop Stand II |
| Reagent A100, 500 ml | |
| Reagent B, 500 ml | |
| Solution 10 – Lysis Buffer, 100 ml | |
| Solution 17 – Blood Lysis Buffer 25 ml | |
| Service Plan |
*Note: Purchase the Consumables and Accessories directly in our WebShop.
Find Via1-Cassette™ COAs in the webshop
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