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NucleoCounter® NC-200™

Automated cell counter

One cassette. One setting. Truly objective

 

  • Consistent cell counting and analysis
  • GMP and 21 CFR Part 11 ready
  • Used with Via1-Cassette™ to eliminate human error
  • No calibration required
  • Unlimited software licenses
NucleoCounter® NC-200™ automated cell counter
Automated cell counter - NucleoCounter NC-200
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The NucleoCounter® NC-200™ is a high precision automated cell counter utilizing state-of-the-art image cytometry. It counts suspension cells, aggregated cells, CAR-T cells, stem cells and cells on microcarriers.

With user-adaptable protocols and specialized assays, the NucleoCounter® NC-200™ provides an all-in-one solution for cell counting and cell viability determination.

The Via1-Cassette™ is used with this counter for automated staining and sample handling. The cassette is volume calibrated to ensure high precision and cell reproducibility.

Combining simplicity and innovation in automated cell counting

Eliminate human bias in cell counting – Via1-Cassette™

NC-200™ automated cell counter vs conventional counters

During manual cell count, the subjective evaluation of the definition of a cell introduces bias to the result. The automatic cell counter NC-200™ is designed to limit human interference in counting. With the Via1-Cassette™, all errors introduced during pipetting and staining are avoided. The Via1-Cassette™ includes an in-built pipette and the immobilized fluorescent dyes acridine orange and DAPI automatically stain the total and dead cell populations, respectively.

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Cell count presentation and data management

 

NC-200™ automated cell counter software view

The NucleoView™ software allows visual inspection of the fluorescence image and the opportunity to verify the cell count. Specific event populations can be selected in the scatter plots and examined visually to determine the validity of their inclusion or exclusion from the final cell counting results. The adapted protocol can be then saved for future measurements, increasing reproducibility.

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GMP Ready Automated Cell Counter

 

NC-200™ automated cell counter across users

The data produced with the NucleoCounter® NC-200™ are traceable and saved within our intuitive NucleoView software™. The optional 21 CFR Part 11 license includes GMP ready features such as logging in with user-group credentials which documents actions in a secured audit trail format. The NucleoCounter® NC-200™ is easy to clean.

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A safe, non-toxic cell counting system

A closed cell counting system that uses self-contained disposable cassettes, helps the user stay protected when handling samples. No pipettes, no exposure to (toxic) reagents, and minimal clean-up make the NucleoCounter® system a safe option.

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Automated Cell Counter NucleoCounter® NC-200™

Counting cells in clumps with NucleoCounter® NC-200™

With the Aggregated Cells Assay, the NucleoCounter® NC-200™ can count cells even from a sample of highly aggregated cells or cells in clumps.

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Total cell count and viability of microcarrier cultures

The NucleoCounter® NC-200™ offers a unique protocol to determine the total cell count and viability of cell cultures grown on microcarriers.

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Counting of mesenchymal stem cells in regenerative medicine

With the NucleoCounter® NC-200™, it is easy to precisely determine viability and cell count of MSC’s derived from different sources e.g. lipoaspirates and bone marrow aspirates.

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Counting of peripheral blood mononucleated cells  in whole blood samples

With the automatic cell counter NC-200™, the total viability and cell count of leukocytes can easily be determined in cell samples containing both platelets and red blood cells.

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21 CFR Part 11 Ready

The NucleoCounter® NC-200™ is designed to be easily implemented in 21 CFR Part 11 compliant laboratories. Key features include control of user access and data export, and secure audit trail.

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Monitoring the manufacturing of CAR-T cells

The NucleoCounter® NC-200™ provides robust and accurate determination of cell count and viability of leukocytes and T cells during adoptive CAR-T cell manufacturing.

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Automated Cell Counter NucleoCounter® NC-200™ overview

  • Tech Specifications
  • Downloads
  • Consumables/Accessories

Tech Specifications

Technical Specifications
Product number 900-0201
Loading volume App. 60 μl loaded into the Via1-Cassette™
Measurement volume 1.4 μl (volume-calibrated Via1-Cassette™)
Analysis time 50 seconds (one step), 120 seconds (two step)
Optimal range 5×104 to 5×106 cells/ml
Size 38 × 26 × 22 cm (W × H × D)
Weight 3 kg / 6.7 lb
Software NucleoView™ software

Downloads

Brochures
No. Brochure Name Download
990-0201 NucleoCounter® NC-200™ – Product Brochure Download
990-0209 NucleoCounting Microcarriers Download
990-0210 NucleoCounting Download
990-0211 NuceloCounting CAR-T Cells Download
990-0401 Service Brochure Download
User guides
No. Document Name Download
991-0203 NucleoCounter® NC-200™ Instrument User Guide Download
991-0205 NucleoCounter® NC-200™ Quick User Guide Download
991-0204 NucleoCounter® NC-200™ NucleoView™ Software User Guide Download
991-0211 Guide for 21 CFR part 11 on NucleoView NC-200™ Download
Application Notes
No. Assay Name Download
994-0220 Viability and Cell Count – Mammalian Cells Open PDF
994-0221 Viability and Cell Count – Microcarrier Cultured Cells Open PDF
994-0203 Viability and Cell Count – Aggregated Mammalian Cells Open PDF
994-0207 Viability and Cell Count – Whole Blood Assay Open PDF
994-0214 Viability and Cell Count – with Reagent A100 and B Open PDF
994-0209 994-0209 Tech Note PQ NC-200 Open PDF
994-0210 994-0210 Tech Note PQ NC-200 (checklist) Open PDF
Documentation
No. Document Name Download
994-0218 Performance of NucleoCounter® NC-200™ Open PDF
994-0216 NucleoCounter® NC-200™ – Variation and Statistics Open PDF
994-0208 White Paper 21 CFR Part 11 for NC-200™ Software Open PDF
994-0031 LED Life Time Considerations for the NucleoCounter® NC-200™ Open PDF
/ Certificate of Conformity Open PDF

Consumables/Accessories

Consumables Accessories
Via1-Cassette™, 1 Box (100 pcs.) Laptop Stand II
Reagent A100, 500 ml
Reagent B, 500 ml
Solution 10 – Lysis Buffer, 100 ml
Solution 17 – Blood Lysis Buffer 25 ml
Service Plan

*Note: Purchase the Consumables and Accessories directly in our WebShop.

Find Via1-Cassette™ COAs in the webshop

Instruments

Xcyto® 5
NucleoCounter® NC-3000™ NucleoCounter® NC-202™ NucleoCounter® NC-200™ NucleoCounter® SP-100™ NucleoCounter® YC-100™

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