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  • NC-3000™
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Automated Cell Analyzer NucleoCounter® NC-250™

  • Analyze total cell count and viability for 8 samples in 3 minutes
  • 5 min cell cycle assay and 1 min apoptosis assay available
  • High-speed and high precision automated cell count

The NucleoCounter® NC-250™ is an automated cell analyzer utilizing fluorescence imaging to characterize cell properties. The NC-250™ can perform high speed cell count and viability determination, a unique 5-minute cell cycle assay and a 1 minute apoptosis assay. The accompanying intuitive software can be used for data analysis of the results and has unlimited licenses. The NC-250™ benchtop image cytometer ensures high precision while being calibration free.

NC-250™ Key features:

  • 8 viability and cell counts in 3 minutes
  • Up to 8 advanced analyses in one run
  • Fast and easy operation
  • Excellent reproducibility
  • No calibration
  • 21 CFR Part 11 ready
  • Automated PDF reports

NucleoCounter® NC-250™ – Precise powerful cytometry

Cell Counting assays

With the combination of simplicity and innovation in the NucleoCounter® NC-250™, counting and viability determination of mammalian cells have never been more uncomplicated. Simply mix a small volume of cell suspension with fluorescent dyes, load and run. Within 3 minutes you will have 8 high precision viability and cell count determinations.

With the NC-250™, 8 viability and cell counts can be performed in less than 3 minutes. Only 10 µL of a mixture of cell suspension and fluorescent dyes is loaded to the chambers in A8 slides. For cells with low concentration or for even higher precision, the A2 slides with 30 µL loaded cell mixture can be used.

With the NucleoCounter® NC-250™, ChemoMetec A/S offers a wide range of optimized protocols meeting the needs of all mammalian cell types grown under different conditions. Through unique assays cell count can be performed even on cells growing on microcarriers and in sphere cultures.

Find protocols here

Cell count presentation and data management

Even for simple cell count and viability assays, it is essential to evaluate the obtained result. With the intuitive NucleoView™ software for NucleoCounter® NC-250™, it is possible to define the counted events precisely. The linkage between images and plots showing fluorescence intensity allows for a precise definition of the events to count – for both total and dead cell populations.

 

The accompanying NucleoView™ software allows for easy coupling between the obtained image and plots showing fluorescence for precise determination of the events to count.

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Measure apoptosis in less than 1 minute

The vitality assay in NucleoCounter® NC-250™ is an extremely fast and simple assay for evaluation of cellular health, oxidative stress and detection of late-stage apoptosis. The fluorescent dye VitaBright 48™ detects reduced thiols such as e.g. glutathione. During late-stage apoptosis, the level of reduced thiols decreases resulting in a lower VitaBright 48™ intensity. With the accompanying user-friendly software for NC-250™, advanced data analysis can effortlessly be performed. The unique possibility to link between images, histograms and scatterplots allows for detailed and precise data analysis.

Example of data analysis of YAC-1 cells grown in the absence (upper row) or presence of camptothecin, CPT (lower row). Cells were stained with VB-48™ and Propidium Iodide (PI) and analyzed using the vitality assay and a NucleoCounter® NC-250™. Scatter plots and histograms were obtained from the NucleoView™ NC-250™ software.

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Content related to the NucleoCounter® NC-250™

Cell cycle analysis

Cell cycle analysis is the most fundamental method to establish anomalies in cell division. With ChemoMetec’s unique 2-step cell cycle a cell cycle can be obtained in 5 minutes including treatment of cells!

Read More

21 CFR part 11 Ready

The NucleoCounter® NC-250™ can easily be implemented in 21 CFR part 11 compliant laboratories. The NucleoView™ NC-250™ software comes with a secure audit trail and control of user access and data export.

Read More

Cell count of microcarrier cultures

The NucleoCounting method employ two reagents that rapidly release nuclei from the microcarriers. The released nuclei is loaded into a disposable Via1-Cassette™ and inserted into the NucleoCounter®.

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Counting nucleated cells  in whole blood

The NucleoCounter® instruments are a rapid, cost-effective alternative to conventional hematology analyzers to measure the nucleated cell count and viability in whole blood.

Read More

NucleoCounter® NC-250™ overview

  • Tech Specifications
  • Downloads
  • Consumables/Accessories

Tech Specifications

Technical Specifications
Product number 900-0251
Loading volume 30 μl for A2-slides, 10 μl for A8-slides
Measurement volume Viability and Cell Count Assay: 11.2 μl for A2-slides, 2.4 μl for A8-slides
Analysis time Viability and Cell Count Assay: 3 minutes for 8 samples in one A8-slide 2 minutes for 2 samples in one A2-slide
Optimal range Viability and Cell Count Assay: 5 x 104 to 5 x 106 cells/ml
Size 19 x 29 x 32 cm / 8 x 12 x 13 in. (W x H x D)
Weight 9 kg / 20 lb
Software NucleoView™ NC-250 software

Downloads

Brochures
No. Name Download
990 0251 NC-250™ Brochure UK format Download pdf
990 0254 NC-250™ Brochure US format Download pdf
990 0255 NC-250™ Cell Cycle Assays Download pdf
990 0256 NC-250™ Vitality Assay Download pdf
990 0257 NC-250™ Viability and Cell Count Assay Download pdf
Application Notes
No. Cell Cycle Assay Download
994 0254 Two-step Cell Cycle Analysis Download pdf
994 0255 Cell Cycle Analysis of Fixed Cells Download pdf
No. Viability and Cell Count Assay Download
994 3815 Cell Count of Microcarrier Cultured Cells Download pdf
994 0258 Mammalian Cells – Viability and Cell Count using NC-Slides Download pdf
994 0259 Count of Aggregated Mammalian Cells using the NC-Slide Download pdf
994 0260 Viability and Cell Count of Aggregated Mammalian Cells using NC-Slide A2™ Download pdf
994 0261 Whole Blood – Viability and Cell Count using NC-Slides Download pdf
994 0262 Counting Aggregated Cells using the NC-Slides with Reagent A100 and B Download pdf
994 0263 Viability and Cell Count using NC-slides A2™ with Reagent A100 and B Download pdf
994 0273 Mammalian Cells – Viability and Cell Count – Method 2 using NCSlides™ Download pdf
No. Vitality Assay Download
994 0256 Vitality assay: Analysis of the level of cellular thiols using the NucleoCounter® NC-250™ system Download pdf
Documentation
No. Name Download
991 0251 Instrument Users Guide Download pdf
991 0252 Software Users Guide Download pdf
991 0253 Quick Users Guide Download pdf
994 0032 LED Considerations Download pdf
991-0256 Guide for 21 CFR Part 11 Download pdf
994 0264 Performance of NucleoCounter NC-250™ Download pdf
994 0267 NC-250™ Variation and Statistics Download pdf
Certificate of Conformity NucleoCounter NC-250™ Download pdf

Consumables/Accessories

Consumables Accessories
Solution 18 – AO•DAPI – 1 ml (200 analysis) Finnpipette 20-200 μl
NC-Slide A2™ – box with 25 Slides PQ Test Kit for NC-250™
NC-Slide A8™ – box with 25 Slides Finntip 250, 96 pcs.
Reagent A100, 500 ml NC-250™ IQ/OQ Kit
Reagent B, 500 ml
Solution 10 – Lysis Buffer, 100 ml
Solution 17 – Blood Lysis Buffer 25 ml
Solution 3 – DAPI Staining Solution – 25 ml
Solution 6 – VB-48™ – PI – 1 ml
Solution 11 – Stabilization Buffer , 100 ml
Solution 12 – DAPI, 1ml

*Note: Purchase the Consumables and Accessories directly in our WebShop.

White Papers
No. Document Name Download
990-0271 NucleoCounter® NC-250™, NucleoView™ NC-250™ Software and Code of Federal Regulation 21 Part 11; Electronic Records, Electronic Signatures (21 CFR Part 11) Download pdf

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