Mammalian Cell Counter NucleoCounter® NC-100™
- Precise, robust and fast determination of cell count and viability
- Simple and easy operation
- Minimize user variation in cell count
The NucleoCounter® NC-100™ is an image cytometer based on fluorescence from the fluorescent dye, propidium iodide (PI). The NC-100™ eliminates human subjectivity and can count even the most aggregated mammalian cells using the NucleoCassette™. The NucleoCassette™ itself is calibrated and severely limits user interference making the NC-100™ very suitable for GMP.
The NucleoCounter® NC-100™ can be connected to a PC using the USB port on the rear. It must be noted that all calculations, cell counting and data analysis is performed in the NC-100™ which is a true stand-alone instrument. The transfer of image data to a PC provides the user with additional documentation and options.
The NucleoCounter® NC-100™ Key benefits:
- Easy operation
- 30 sec. analysis time
- No cleaning or calibration
- Excellent reproducibility
- Safe sample handling and disposal
- Excellent for aggregated cells
Determination of cell viability with NucleoCounter® NC-100™
The NucleoCounter® NC-100™ concept offers a determination of cell viability in addition to the total count of cells in the sample. Determination of viability is based on the “PI-exclusion” method.
The cell viability of a sample is determined using the total cell count and the count of non-viable cells (see the figure below).
When a sample is mixed with Reagent A100 and Reagent B a lysis of the viable cell membrane takes place rendering all the cell nuclei susceptible to staining with propidium iodide (PI) resulting in a total cell count.
Non-viable cells, however, are permeable without treatment and are therefore stained directly with PI, resulting in a non-viable cell count.
NucleoCounter® NC-100™ overview
|Measurement volume||2 μl in the measurement chamber of the NucleoCassette™|
|Analysis time||30 seconds|
|Size||38 x 26 x 22 cm (W x H x D)|
|Weight||3 kg / 6.7 lb|
|Software||NucleoView™ computer software for documentation and presentation – optional|
|Printer||External printer for documentation – optional|
|NucleoCounter® NC-100™ Application Notes|
|994 0035||A Note on Analytical Performance||Open PDF|
|994 0003||Cell counts and viability – Reagent A100||Open PDF|
|994 0005||Dilution of the samples||Open PDF|
|994 0007||Single-cell-suspension: Cell counts and viability – Reagent C100||Open PDF|
|994 0017||Total cell count and viability of microcarrier cell cultures||Open PDF|
|994 0019||Protocol for enzymatic nuclei dissociation of multi-layered hepatocytes – Total cell counts||Open PDF|
|994 0034||Cell counts – whole blood||Open PDF|
|NucleoCounter® NC-100™ Documentation|
|991 0009||User’s guide v1.3||Open PDF|
|991 0006||NucleoView™ User’s Guide||Open PDF|
|994 0014||NucleoCounter® System: Connecting a PCNEOS Thermal printer||Open PDF|
|994 0016||Cleaning Guide for NucleoCounter® Family||Open PDF|
|994 0030||LED Life Time Considerations for the NucleoCounter® Family||Open PDF|
|Certificate of Conformity: NucleoCounter® NC-100™||Open PDF|
*Note: Purchase the Consumables and Accessories directly in our WebShop.