• Investor Relations
  • About us
  • Contact us
  • Careers
    • Life Science Field Support Technician – United States
    • Field Application Scientist – United States
    • Field Application Scientist, APAC
    • Field Application Scientist – DACH
  • Support
  • German
  • French
  • Japanese
  • Products
    • NucleoCounter® NC-3000™
    • NucleoCounter® NC-202™
    • NucleoCounter® NC-200™
    • NucleoCounter® SP-100™
    • NucleoCounter® YC-100™
    • Xcyto®
    • Via1-Cassette & Via2-Cassette
    • NucleoView™ Software
    • Consumables
    • Accessories
  • Solutions
    • Cell Therapeutic Applications
    • Stem Cell Research
    • Cancer Cell Research
    • Bioprocessing
    • Toxicology
    • Cell Biology Research
    • Sperm Cell Counting
    • Yeast Cell Counting
  • Technical Resources
    • Application Notes
    • Articles
    • Assays
    • Cell types
    • Customer projects
    • Posters
    • Publications
    • Testimonials
    • Videos
    • Webinars
Webshop
  • Products
    • NucleoCounter® NC-3000™
    • NucleoCounter® NC-250
    • NucleoCounter® NC-200™
    • NucleoCounter® NC-100
    • NucleoCounter® SP-100
    • NucleoCounter® YC-100
    • NucleoCounter® SCC-100
    • NucleoCounter® SCC-400
  • Applications
    • Cell counting in cell therapeutic applications
    • Stem Cells Counting
    • Bioprocessing
    • Toxicology
    • Research
    • Cancer Cell Counting
    • Sperm Cell Counting
    • Yeast Cell Counting
    • Somatic Cell Counting
  • Resources
  • Investor Relations
  • About us
  • Contact
  • Careers
    • Senior Sales Representatives USA
    • Field Application Scientist USA
    • Vertriebsmitarbeiter im Außendienst (m/w) (Germany)
    • Field Application Scientist, Germany
    • Sales Manager, United Kingdom
    • Chinese speaking Field Application Scientist, Denmark
  • Webshop
  • NC-3000™
  • NC-202™
  • NC-200™
  • SP-100™
  • YC-100™
  • Via1-Cassette & Via2-Cassette
  • NucleoView™ Software

NucleoCounter® NC-3000™

Advanced image cytometer

  • Variety of ease of use assays
  • Cell cycle analysis in 5 minutes
  • 1 minute apoptosis assay
  • GFP assay in 1 minute
  • Advanced high-speed cell count
  • Open platform with 5 channels
NucleoCounter® NC-3000™ Advanced Image Cytometer

NucleoCounter® NC-3000™ Advanced Image Cytometer

Request a quote Request a demo Contact us Technical specifications

Request a Quote

Request a Free Trial

The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties. The NC-3000™ can perform high-speed cell count and viability determination and includes plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute cell cycle assay and a selection of apoptosis assays.

With the FlexiCyte™ license, the user can create protocols choosing light source, emission filters and detailed settings. The accompanying intuitive software can be used for data analysis of the results and has unlimited licenses.

The NucleoCounter® NC-3000™ advanced image cytometer ensures flow-like analysis with high reproducibility and is calibration free.

Key features:

  • No rinsing or clogging
  • Easy to operate – no warm-up or calibration
  • CFR 21 Part 11 ready
  • Automated PDF reports
  • Unlimited software licenses and free software updates
  • Excellent support by field application specialists
  • High-speed cell counting and advanced cell analysis
  • Visual inspection of cells – plot to image
  • Images are saved automatically and can be re-analyzed later
  • Low sample volume
  • Build your own assays with up to 4 analytical channels
  • Export data to FCS Express, FlowJo, etc.

Cytometry analysis in the cell lab

NucleoCounter® NC-3000™ Advanced Image Cytometer

Visual verification of the count

When counting manually the scientist can visually verify the counting. This verification of the count can likewise be performed with the intuitive NucleoView™ software included with the NC-3000™. Scatter plots and images are interlinked making it possible to verify cell populations visually.

NucleoCounter® NC-3000™ Advanced Image Cytometer software view

The accompanying NucleoView™ software allows for easy coupling between the obtained image and plots showing fluorescence for precise determination of the events to count.

Read More

Counting cells in clumps with NucleoCounter® NC-3000™

The NucleoCounter® NC-3000™ can count cells even from a sample of highly aggregated cells or cells growing in clumps. In the Aggregated Cells Assay, the total cell count derives from a lyzed aliquot of the sample (see schematic representation below). Lysis disrupts the cell membrane, dismantles clumps and releases nuclei into suspension. Nuclei are stained by DAPI and automatically counted. Because dead cells are typically less abundant and tend to detach from aggregates, the dead cell count is determined from the non-lyzed aliquot (figure below). The combination of these two measurements results in high precision and accuracy for even the most difficult clumps.

Read More

Cell cycle analysis

Cell cycle analysis is the most fundamental method to establish anomalies in cell division and thereby symptoms of cell damage. With ChemoMetec’s unique 2-step cell cycle a cell cycle can be performed in 5 minutes including treatment of cells! A cell cycle profile will readily be displayed in the accompanying PlotManager software and percentage or numbers of events in G0/G1, S-phase, G2 and sub-G1 can be identified.

Read More

A safe, non-toxic cell counting system

A closed cell counting system that uses self-contained disposable cassettes, helps the user stay protected when handling samples. No pipettes, no exposure to (toxic) reagents, and minimal clean-up make the NucleoCounter® system a safe option.

Read More

Content related to the NucleoCounter® NC-3000™ advanced image cytometer

Quantification of GFP transfected cells

NucleoCounter® NC-3000™ Advanced Image Cytometer assay view

With the NucleoCounter® NC-3000™ plug-and-play GFP transfection assay, the number of viable and dead GFP-transfected cells can be determined with high precision.

Read More

21 CFR Part 11 Ready

NucleoCounter® NC-3000™ Advanced Image Cytometer with software

The NC-3000™ is ideal for regulated QC, production and even PD and R&D departments. The instrument and accompanying software can easily be integrated into existing IT networks.

Read More

User adaptable protocols with FlexiCyte™

The FlexiCyte™ module enables users to perform advanced cell analysis of a broad range of biomarkers and fluorescent proteins in mammalian cells.

Read More

Counting of Mesenchymal Stem Cells

Easy and precise determination of viability and cell count of primary mesenchymal stem cells derived from lipoaspirates and bone marrow aspirates.

Read More

NucleoCounter® NC-3000™ advanced image cytometer overview

  • Assays
  • Tech Specifications
  • Downloads
  • Consumables/Accessories

Assays

Assays
Assay Name
Annexin V Assay
Caspase 3/7, 8, 9 Assay
Cell Cycle Assays
DNA Fragmentation Assay
GFP Transfection Efficiency Assays
Mitochondrial Potential Assay
Cell Viability and Cell Count Assays
Vitality Assay
FlexiCyte™ Assay

Tech Specifications

Technical Specifications
Product number 970-3002 (Fixed Analytical Assays)
970-3003 (FlexiCyte™)
Optics Lens with x 2 magnification, 1/2” CCD with 1392 x 1040 pixels
Sample device Via1-Cassette™, NC-Slide A2™ and NC-Slide A8™
Excitation (nm) Seven LED light sources with peaks at 365, 405, 475, 530, 630 + 2 white LEDs
Emission (nm) Nine interchangeable emission filters: 430/20, 470/55, 475/15, 530/15, 560/35, 580/25, 675/75, 630 LP, 740/60
Sample consumption Approximately 60 μl (cassettes), Approximately 30 μl (A2 slides), Approximately 10 μl (A8 slides)
Analyzed volume 3.2 μl sample (cassettes), 0.8-16 μl sample (chamber slides)
Optimal Range 5 x 104 – 5 x 106 cells/ml (for counting)
Cell types Mammalian cells, yeast, insect cells, avian cells
Minimum requirements Windows 7, 8 or 10, USB 2.0, screen resolution 1366 x 768 pixels, 4 GB RAM and 10 GB free disc space
Data presentation Images, tables, histograms, scatter plots
Weight/Dimensions 14 kg (30.9 lb); 29 x 29 x 31 cm [H x W x D]
Supply Voltage 100-240V~ 50-60Hz
Power Consumption 5/50W (power save mode/peak)

Downloads

Brochures
No. Brochure Name Download
990-0312 NucleoCounter® NC-3000™ Brochure Download .pdf
990-0313 NucleoCounter® NC-3000™ Brochure (US letter format) Download .pdf
990-0301 NC-3000™ Vitality Assay Download .pdf
990-0302 NC-3000™ Cell Cycle Download .pdf
990-0303 NC-3000™ Viability and Cell Count Assay Download .pdf
990-0304 NC-3000™ DNA Fragmentation Assay Download .pdf
990-0305 NC-3000™ Mitochondrial Potential Assay Download .pdf
990-0306 NC-3000™ GFP Transfection Efficiency Assay Download .pdf
990-0308 NC-3000™ Annexin V Assay Download .pdf
990-0309 NC-3000™ Caspase 3/7 8 9 Assay Download .pdf
Application Notes
No. Cell Cycle Assay Download
994 3017 Annexin V Assay using the NucleoCounter® NC-3000™ System Download pdf
994 3021 Caspase assay using the Fluorochrome-labeled inhibitor of caspases assay (FLICA) FAM-DEVD-FMK (caspase 3/7), FAM-LETD-FMK (caspase 8) or FAM- LEHD-FMK (caspase 9) Download pdf
994 3001 Two-step cell cycle analysis Download pdf
994 3002 Cell cycle analysis of fixed cells Download pdf
994 3003 DNA fragmentation assay Download pdf
994 3024 FlexiCyte™ Protocol for the NucleoCounter® NC-3000™ system Download pdf
994 3023 GFP transfection efficiency assay using Hoechst 33342 and propidium iodide Download pdf
994 3008 Mitochondrial potential assay using the NucleoCounter® NC-3000™ system Download pdf
994 3005 Analysis of the level of cellular thiols using the NucleoCounter® NC-3000™ system Download pdf
No. Viability and Cell Count Assay Download
994-3006 Viability and Cell Count using the Via1-Cassette™ – Mammalian cells Download pdf
994-3007 Count of PI Stained Cells – Total Count of Mammalian Cells Download pdf
994-3010 Viability and Cell Count using NC-Slides™ – Mammalian Cells Download pdf
994-3011 Viability and Cell Count using the Via1-Cassette™ – Insect cells Download pdf
994-3012 Viability and Cell Count using NC-Slides™ – Insect cells Download pdf
994-3014 Viability and Cell Count using the Via1-Cassette™ – Purified Leukocytes Download pdf
994-3015 Counting of Aggregated Mammalian Cells using the Via1-Cassette™ Download pdf
994-3016 Counting of Aggregated Mammalian Cells using NC-Slides™ Download pdf
994-3018 Viability and Cell Count using the Via1-Cassette™ – Aggregated Mammalian Cells Download pdf
994-3019 Viability and Cell Count using NC-Slide A2™ – Aggregated Mammalian Cells Download pdf
994-3026 Whole Blood – Viability and Cell Count using NC-Slides™ Download pdf
994-3027 Counting Aggregated Cells using NC-Slides™ with Reagent A100 and B Download pdf
994-3028 Viability and Cell Count using Via1-Cassette™ with Reagent A100 and B Download pdf
994-3029 Viability and Cell Count using NC-slides A2™ with Reagent A100 and B Download pdf
994-3030 Counting Aggregated Cells using the Via1-Cassette™ with Reagent A100 and B Download pdf
994-3032 Count and Viability of PI Stained Mammalian Cells Download pdf
994-3034 Viability and Cell Count Method 2 using the Via1-Cassette™ – Mammalian cells Download pdf
994-3035 Viability and Cell Count Method 2 using NC-Slides™ – Mammalian Cells Download pdf
No. Yeast Count and Viability Assay Download
994-3009 Count of PI Stained Cells – Total Count of Yeast Cells Download pdf
994-3031 Count and Viability of PI Stained Yeast Cells Download pdf
Documentation
No. Document Name Download
990-3001 NC-3000™ User Guide Download .pdf
990-3003 NucleoView NC-3000™ Software User Guide Download .pdf
990-3004 FlexiCyte™ User Guide Download .pdf
990-0033 LED Life Time Considerations for the NC-3000™ Download .pdf
990-0304 NC-3000™ Variations and Statistics Download .pdf
990-0306 NC-3000™ Certificate of Conformity Download .pdf
White Papers
No. Document Name Download
990-3804 NucleoView™ NC-3000™ Software and Code of Federal Regulation 21 Part 11;
Electronic Records, Electronic Signatures (21 CFR Part 11)
Download pdf
Articles
No. Name
1 Quantitative assesment of apoptotic events using image and flow cytometry
2 A comparison of DNA quantitation by image and flow cytometry
3 List of Publications on NC-3000™

Consumables/Accessories

Consumables Accessories
Via1-Cassette™, 1 Box (100 pcs.) Finnpipette 20-200 μl
PI-Cassette™, 1 box (100 pcs.) PQ Test Kit for NC-3000™ – Via1-Cassette™
NC-Slide A2™ – box with 25 Slides PQ Test Kit for NC-3000™ – Slides
NC-Slide A8™ – box with 25 Slides NC-3000™ OQ Test Kit 1
Reagent A100, 500 ml Finntip 250, 96 pcs.
Reagent Y100, 500 ml
Solution 2 – Propidium Iodide Staining Solution – 25 ml
Solution 3 – DAPI Staining Solution – 25 ml
Solution 5 – VB-48™ – PI – AO – 1 ml
Solution 7 – JC-1 – 1ml
Solution 8 – DAPI – PBS Staining Solution – 25 ml
Solution 10 – Lysis Buffer, 100 ml
Solution 11 – Stabilization Buffer , 100 ml
Solution 12 – DAPI, 1ml
Solution 13 – AO – DAPI – 1 ml
Solution 15 – Hoechst – 1 ml
Solution 16 – Propidium – 1 ml
Solution 17 – Blood Lysis Buffer 25 ml
Solution 19 – VitaBright-43™ – 1 mL
Solution 20 – VitaBright-48™ – 100 µl

*Note: Purchase the Consumables and Accessories directly in our WebShop.

Instruments

Xcyto® 5
NucleoCounter® NC-3000™ NucleoCounter® NC-202™ NucleoCounter® NC-200™ NucleoCounter® SP-100™ NucleoCounter® YC-100™

Sales

Request Demo
Request Quote

WebShop

Buy reagents and more

Contact

Contact
Find distributors
Register NucleoCounter®

Support/Help

Science Support Center

Follow us

  • FacebookFacebook
  • LinkedInLinkedIn
  • TwitterTwitter
  • YouTubeYouTube

Terms & Conditions

Sales Terms and Conditions - Europe and ROW

Sales Terms and Conditions - USA and Canada

ChemoMetec A/S © 1997-2020 All Rights Reserved
Gydevang 43, DK-3450 Allerod, Denmark | Tel: +45 48131020 | Fax: +45 48131021 | mail: contact@chemometec.com
CVR: 19828131 | LEI: 213800533NBKECGONO68
www.chemometec.com| www.chemometec.de| www.chemometec.fr EXTRANET
This site uses cookies. By continued use of our site, you accept our websites use of cookies. Find out more.