Somatic Cell Counter – NucleoCounter® SCC-400™
Counts up to 200 samples/h, plus:
- No cleaning or calibration
- Excellent reproducibility
- High precision, working factor 85
The NucleoCounter® SCC-400™ Somatic Cell Counter offers unique ease of use and effective and fast determination of the number of somatic cells in a milk sample.
The compact instrument fits perfectly in any research, reference or central laboratory function. The NucleoCounter® SCC-400™ is very simple to operate with only limited training in laboratory work.
- Easy operation
- Up to 200 samples per hour
- Simple cleaning
- Excellent reproducibility
Repeatibility Error
The figure shows observed repeatability error of three NucleoCounter® SCC-400™ instruments. The solid line in the graph shows the expected Poisson error, considering only counting error. These results demonstrate the good repeatability of the NucleoCounter® SCC-400™ method.
Comparison SCC-400™ vs. Flow Cytometer
Raw cow milk samples were measured on NucleoCounter® SCC-400™ and “Industry Standard” Flow Cytometer. Samples were heated to 40°C and measured on the Flow Cytometer. 250 µL of a milk sample at room temperature was mixed with 500 µL of Reagent C400 and measured on the NucleoCounter® SCC-400™. The figure demonstrates the excellent correlation between the two methods in the range up to at least 7,000,000 Cells/mL.
NucleoCounter® SCC-400™ overview
| SCC-400™ Technical Specifications | ||
|---|---|---|
| Product number | 900-0400 | |
| Analyte | Somatic Cells in Raw Milk | |
| Analytical Principle | Fluorescence Microscopy | |
| Sample Capacity | Up to 200 samples per hour | |
| Sample Volume | 0.25 ml milk mixed with 0.5 ml Reagent C400 | |
| Sample Temperature | 20 to 42°C | |
| Performance Range | 0 to 2,000,000 cells/mL | |
| Measured Volume | 12 µL of milk is analyzed1) | |
| Working Factor | 85 1) | |
| Accuracy | < 10% Relative mean difference from DMSCC 2)
< 2% Relative mean difference from NucleoCounter® SCC 3) |
|
| Repeatability 1) 4) | CV < 5% at 100,000 cells/mL
CV < 3.5% at 300,000 cells/mL CV < 3% at 500.000 cells/mL |
|
1) Instrument in ‘High Precision Mode’
2) Direct Microscope Somatic Cell Count
3) NucleoCounter® SCC instruments, including NucleoCounter® SCC-100™ and NucleoCounter® SCC-400™
4) Repeatability as relative coefficient of variance
| NucleoCounter® SCC-400™ Brochures | |
|---|---|
| Brochure Name | Download |
| NucleoCounter® SCC-400™ – Application Flyer | Download PDF |
| NucleoCounter® SCC-400™ – Technical Specifications | Download PDF |
| NucleoCounter® SCC-400™ Application Notes | ||
|---|---|---|
| No. | Assay Name | Download |
| 994 0021 | Determination of SCC in Milk using the NucleoCounter® SCC-400™ System | Download PDF |
| NucleoCounter® SCC-400™ Documentation | ||
|---|---|---|
| No. | Document Name | Download |
| 991 0400 | User’s guide | Download PDF |
| 994 0036 | LED Life Time Considerations for the NucleoCounter® SCC-400™ |
Download PDF |
| Consumables | Accessories |
|---|---|
| Reagent C400 | Finnpipette 20-200 μl |
| Finnpipette 100-1000 µl | |
| Finntip 250, 96 pcs. | |
| Finntip 1000, 96 pcs. | |
| UL Power Supply SPN260-12 |
*Note: Purchase the Consumables and Accessories directly in our WebShop.
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