Somatic Cell Counter NucleoCounter^® SCC-100™

The inherent properties of the ChemoMetec Image Cytometry implementation offer unique cell counting properties, the most important one being outstanding reproducibility performance. Each instrument is calibrated during production and the durable properties of the LED light source, optical system and the image capturing camera means that no additional calibration is required, neither short-time nor long-time calibration.

This is demonstrated in the histogram below which shows the results of the comparison between 51 NucleoCounter^® SCC-100™ instruments and a NucleoCounter^® SCC-100™ reference instrument. The reproducibility was tested by measuring a number of milk samples on each of the instruments being tested as well as on the reference instrument. The histogram illustrates the observed relative difference in Cell Count, where the observed mean difference is -0.011% and the standard deviation of differences of 0.46%, which is comparable with the expected standard deviation of the mean, calculated to be 0.65%.

Raw cow milk samples were measured on NucleoCounter^® SCC-100™ and “Industry Standard” Flow Cytometer. Samples were heated to 40°C and measured on the Flow Cytometer. 500 µL of a milk sample at room temperature was mixed with 500 µL of Reagent C and measured on the NucleoCounter^® SCC-100™. The figure below demonstrates the excellent correlation between the two methods in the range up to 1,000,000 Cells/mL.