Cell Viability and Cell Count Assays
Cell Viability Assay using the NC-3000™
Key benefits:
- Extremely fast and convenient measurement of cell count and viability
- High accuracy and high reproducibility
- Automatic data collection
- Objective analysis
- No calibration required
- Specialized protocols for Purified Leucocytes and Full Blood Samples
- PlotManager for superior data presentation
- Automated PDF reports
- Export of data in FCS/ACS formats
Perform 8 cell viability counts in less than 3 minutes!
Principle
Using fluorescence microscopy and image analysis, the NucleoCounter® NC-3000™ automates the detection of cells. The NC-3000™ Viability and Cell Count Assays use the cell stain Acridine Orange for cell detection, and the nucleic acid stain DAPI for detecting non-viable cells. DAPI cannot penetrate the cell membrane, hence it only stains cells with a permeable cell membrane; the non-viable cells.
The different Viability and Cell Count Assays are optimized for viability and cell counting of mammalian and insect cells, E.g. Purified leucocytes, cells in peripheral blood samples.
For high precision use the Via1-Cassette™ or the NC-Slide A2™ and for screening use the NC-Slide A8™.
Results: Presented in PlotManager
Figure: Superior data presentation by the NucleoViewTM software. Data is presented in scatter plots, plotting the area vs. intensity of the fluorescent signal for Acridine Orange and DAPI, respectively. All stained cells will be inside of the counting gate. The histogram shows the cell diameter. The table shows the total cell count, the live and the dead count, as well as the percent cell viability.
Application Notes (.PDF):
- Viability and Cell Count using the Via1-Cassette™ - Mammalian cells
- Count of PI Stained Cells – Total Count of Mammalian Cells
- Viability and Cell Count using NC-Slides™ - Mammalian Cells
- Viability and Cell Count using the Via1-Cassette™ - Insect cells
- Viability and Cell Count using NC-Slides™ - Insect cells
- Viability and Cell Count using the Via1-Cassette™ - Purified Leukocytes
- Counting of Aggregated Mammalian Cells using the Via1-Cassette™
- Counting of Aggregated Mammalian Cells using NC-Slides
- Viability and Cell Count using the Via1-Cassette™ - Aggregated Mammalian Cells
- Viability and Cell Count using NC-Slide A2™ - Aggregated Mammalian Cells
- Whole Blood - Viability and Cell Count using NC-Slides®
- Counting Aggregated Cells using NC-Slides® with Reagent A100 and B
- Viability and Cell Count using Via1-Cassette™ with Reagent A100 and B
- Viability and Cell Count using NC-slides A2™ with Reagent A100 and B
- Counting Aggregated Cells using the Via1-Cassette™ with Reagent A100 and B
- Count and Viability of PI Stained Mammalian Cells
- Viability and Cell Count Method 2 using the Via1-Cassette™ - Mammalian cells
- Viability and Cell Count Method 2 using NC-Slides® - Mammalian Cells
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