• Investor Relations
  • About us
  • Contact us
  • Careers
    • Life Science Field Support Technician – United States
    • Field Application Scientist – United States
    • Field Application Scientist – DACH
  • Support
  • German
  • French
  • Japanese
  • Products
    • NucleoCounter® NC-3000™
    • NucleoCounter® NC-202™
    • NucleoCounter® NC-200™
    • NucleoCounter® SP-100™
    • NucleoCounter® YC-100™
    • Xcyto®
    • Via1-Cassette & Via2-Cassette
    • NucleoView™ Software
    • Consumables
    • Accessories
  • Solutions
    • Cell Therapeutic Applications
    • Stem Cell Research
    • Cancer Cell Research
    • Bioprocessing
    • Toxicology
    • Cell Biology Research
    • Sperm Cell Counting
    • Yeast Cell Counting
  • Technical Resources
    • Application Notes
    • Articles
    • Assays
    • Cell types
    • Customer projects
    • Posters
    • Publications
    • Testimonials
    • Videos
    • Webinars
Webshop
  • Products
    • NucleoCounter® NC-3000™
    • NucleoCounter® NC-250
    • NucleoCounter® NC-200™
    • NucleoCounter® NC-100
    • NucleoCounter® SP-100
    • NucleoCounter® YC-100
    • NucleoCounter® SCC-100
    • NucleoCounter® SCC-400
  • Applications
    • Cell counting in cell therapeutic applications
    • Stem Cells Counting
    • Bioprocessing
    • Toxicology
    • Research
    • Cancer Cell Counting
    • Sperm Cell Counting
    • Yeast Cell Counting
    • Somatic Cell Counting
  • Resources
  • Investor Relations
  • About us
  • Contact
  • Careers
    • Senior Sales Representatives USA
    • Field Application Scientist USA
    • Vertriebsmitarbeiter im Außendienst (m/w) (Germany)
    • Field Application Scientist, Germany
    • Sales Manager, United Kingdom
    • Chinese speaking Field Application Scientist, Denmark
  • Webshop

Cell Viability and Cell Count Assays

Cell Viability Assay using the NC-3000™

With the NucleoCounter® NC-3000™ advanced image cytometer, mammalian, yeast and even insect cells can easily be counted and viability determined. NucleoCounter® NC-3000™ offers a range of protocols matching the needs for even specialized cell types.
Advanced image cytometer - NucleoCounter NC-3000

Request a Quote
Request a Demo
Contact us

Request a Quote

Request a Demo

Key benefits:

  • Extremely fast and convenient measurement of cell count and viability
  • High accuracy and high reproducibility
  • Automatic data collection
  • Objective analysis
  • No calibration required
  • Specialized protocols for Purified Leucocytes and Full Blood Samples
  • PlotManager for superior data presentation
  • Automated PDF reports
  • Export of data in FCS/ACS formats

Perform 8 cell viability counts in less than 3 minutes!

The Via1-Cassette™ used together with the NucleoCounter® NC-3000™ enables fast and reliable determination of cell count and viability in one simple step. It is suitable for a wide range of cell types such as adherent and suspension cell lines and primary cells. In order to determine viability and cell concentration, a sample of the cell suspension is drawn directly into the Via1-Cassette™. The inside of the Via1-Cassette™ is coated with two different dyes, staining the entire cell population and the non-viable cells, respectively. The Via1-Cassette™ is placed in the NucleoCounter® NC-3000™ where the cell count and viability are determined.

 

Cell Viability Assay and Cell Counting Assay with NC-3000 Diagram Presentation

The NC-Slides A2™ are recommended for improved statistics, and NC-Slides A8™ are recommended for high throughput analysis.

 

Using slides or cassette for Cell Cycle Assay

Principle

Using fluorescence microscopy and image analysis, the NucleoCounter® NC-3000™ automates the detection of cells. The NC-3000™ Viability and Cell Count Assays use the cell stain Acridine Orange for cell detection, and the nucleic acid stain DAPI for detecting non-viable cells. DAPI cannot penetrate the cell membrane, hence it only stains cells with a permeable cell membrane; the non-viable cells.

The different Viability and Cell Count Assays are optimized for viability and cell counting of mammalian and insect cells, E.g. Purified leucocytes, cells in peripheral blood samples.

For high precision use the Via1-Cassette™ or the NC-Slide A2™ and for screening use the NC-Slide A8™.

Results: Presented in PlotManager

PlotManager Results Cell Viability Assay with NC-3000

Figure: Superior data presentation by the NucleoViewTM software. Data is presented in scatter plots, plotting the area vs. intensity of the fluorescent signal for Acridine Orange and DAPI, respectively. All stained cells will be inside of the counting gate. The histogram shows the cell diameter. The table shows the total cell count, the live and the dead count, as well as the percent cell viability.

Application Notes (.PDF):

  • Viability and Cell Count using the Via1-Cassette™ - Mammalian cells
  • Count of PI Stained Cells – Total Count of Mammalian Cells
  • Viability and Cell Count using NC-Slides™ - Mammalian Cells
  • Viability and Cell Count using the Via1-Cassette™ - Insect cells
  • Viability and Cell Count using NC-Slides™ - Insect cells
  • Viability and Cell Count using the Via1-Cassette™ - Purified Leukocytes
  • Counting of Aggregated Mammalian Cells using the Via1-Cassette™
  • Counting of Aggregated Mammalian Cells using NC-Slides
  • Viability and Cell Count using the Via1-Cassette™ - Aggregated Mammalian Cells
  • Viability and Cell Count using NC-Slide A2™ - Aggregated Mammalian Cells
  • Whole Blood - Viability and Cell Count using NC-Slides®
  • Counting Aggregated Cells using NC-Slides® with Reagent A100 and B
  • Viability and Cell Count using Via1-Cassette™ with Reagent A100 and B
  • Viability and Cell Count using NC-slides A2™ with Reagent A100 and B
  • Counting Aggregated Cells using the Via1-Cassette™ with Reagent A100 and B
  • Count and Viability of PI Stained Mammalian Cells
  • Viability and Cell Count Method 2 using the Via1-Cassette™ - Mammalian cells
  • Viability and Cell Count Method 2 using NC-Slides® - Mammalian Cells

Instruments

Xcyto® 5
NucleoCounter® NC-3000™ NucleoCounter® NC-202™ NucleoCounter® NC-200™ NucleoCounter® SP-100™ NucleoCounter® YC-100™

Sales

Request Demo
Request Quote

WebShop

Buy reagents and more

Contact

Contact
Find distributors
Register NucleoCounter®

Support/Help

Science Support Center

Follow us

  • FacebookFacebook
  • LinkedInLinkedIn
  • TwitterTwitter
  • YouTubeYouTube

Terms & Conditions

Sales Terms and Conditions - Europe and ROW

Sales Terms and Conditions - USA and Canada

ChemoMetec A/S © 1997-2020 All Rights Reserved
Gydevang 43, DK-3450 Allerod, Denmark | Tel: +45 48131020 | Fax: +45 48131021 | mail: contact@chemometec.com
CVR: 19828131 | LEI: 213800533NBKECGONO68
www.chemometec.com| www.chemometec.de| www.chemometec.fr EXTRANET
This site uses cookies. By continued use of our site, you accept our websites use of cookies. Find out more.