Cell Aggregation Assays – NucleoCounter^® NC-200™

Figure 1. Determination of the cell concentration of aggregated MCF7 cells. The nuclei from the cells were freed by lysis using Reagent A100 and Reagent B and analyzed using the Count of Aggregated Cells – A100 and B Assay. The total cell population is stained with DAPI and appears blue. An insert shows a close up of parts of the images. The results are presented at the bottom right and extended results are presented in the result tab page.

Aggregated cells lying on top of each other can be very challenging to count. When using trypan blue to stain cells in a hemocytometer or flow based system using brightfield, counting cells or determining the viability of heavily aggregated cells can be impossible if a clump consists of 10, 20, or even more cells. With the NucleoCounter^® NC-200™, counting aggregated cells has never been more precise.

Based on a carefully developed lysis buffer system, ChemoMetec A/S offers an optimized 2-step protocol for counting and determining viability of heavily agggregated cells.

1. In the first step, all cells are lysed and counted based on a fluorescent DNA stain.
2. In the second step, only dead cells are counted. Within just 2 minutes, the NucleoCounter^® NC-200™ will report the precise viabilty and cell count.