Counting aggregated cells
NucleoCounter® instruments offer robust and reliable solutions for counting cells in clumps and aggregates. They use fluorescent dyes to stain cell nuclei and employ a powerful algorithm to segment single nuclei within each smaller aggregate. For the more heavily clumped samples, a numerical estimation of the level of cell aggregation allows the operator to take an informed decision on whether to switch to the dedicated Aggregated Cell Assay, which disassembles cells with a lysis step. This dedicated assay is also used for counting cells grown in microcarriers or spheroids.