Measuring cell count and viability in microcarrier cultures
– with NucleoCounter® automated cell counters
Microcarriers – a scalable culture system for adherent cells
Figure 1. Microcarrier bioreactor. Microcarriers offer a convenient method for growing adherent cells in bioreactors.
Scaling up cell and virus production can be challenging. Cell culture flasks are not feasible for industrial scale production when a thousandfold increase in production volume is needed. Microcarriers offer a convenient method for growing adherent cells in bioreactors. They serve as a scaffold that adherent cells attach to, allowing them to proliferate while a bioreactor keeps the cell-microcarrier complex freely suspended in the media. Therefore, adherent cell lines are grown like suspension cells, simplifying scaling and allowing existing resources to be leveraged for process optimization and production.
The NucleoCounter® method for measuring cell count and viability in microcarrier cultures
Figure 2. NucleoCounter® instruments. The NucleoCounter® method for measuring cell count and viability in microcarrier cultures.
A NucleoCounter® detects cells by staining cell nuclei. The cells are made visible using the fluorescent dye DAPI, which is highly specific to DNA, giving accurate detection of cell nuclei even in the presence of cellular debris. Cell sampling, fluorescent staining and counting chamber loading are combined into a single workflow by the unique Via1-Cassette™, which is loaded into a NucleoCounter® NC-200™ or NC-3000™ , together calculating total cell count and viability.
Save time when counting cells growing on microcarriers
A comparison of the traditional trypsin digestion method and the NucleoCounter® workflow shows that the NucleoCounter® removes several centrifugation, pipetting and incubation steps. The entire cell counting process can be completed in less than five minutes.
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