The NucleoView software presents fluorescent images after auto image scaling of fluorescent intensity. However, fluorescent intensity varies from sample to sample. Hence, images shown in NucleoView software may appear dim for some samples. This can be adjusted by changing the values of image scale manually.
How to adjust image scaling for gating
- Right click on the fluorescent image and choose “Image Scaling”, a “Raw Image Scaling” dialogue window will prompt out.
- Cancel software automatic image scale by deselecting “Auto Scale”.
Delete “*” and adjust the values in the value input boxes.
Channel 0 – Acridine Orange/Green Fluorescence Channel
Channel 1 – DAPI/Blue Fluorescence Channel