Generally, the NucleoCounter instruments needs little cleaning, but depending on the environment that the instruments are located in different measures of cleaning can be applied.
Most commonly the NC200 or NC202 and NC3000/NC250 instrument enclosure needs cleaning.
For the NC3000 and NC250 cleaning of the sample tray is also recommended.
Below is instruction on how to clean the different parts of the NC200 or NC202 and NC3000/NC250.
Cleaning of NC200 or NC202 and NC3000/NC250
When cleaning the enclosure, it is recommended to use a soft moist cloth and gently wipe the surface. Any contamination, which does not come off immediately, should be rubbed gently with a cloth wetted with mild detergent.
Never use organic solvents or aggressive detergents to clean the exterior of the instrument as this might damage the surface.
The cassette fixture does not need cleaning if there is no visible dust or liquid spill. Great care must be taken against introducing any liquid or dust into the Nuclecounter. Any liquid that enters the interior of the instrument can damage the optical parts and thus compromise the quality of the cell counts.
The black surface of the cassette fixture is anodized aluminum and can therefore be wiped off with a clean, dry and dust free cloth that reduces fiber contamination of the cassette fixture.
Spill of liquid
Presence of a contaminant (e.g. dust particles) will normally not influence the counting of cells, because the Nucleocounter instrument will distinguish between cells and contaminants.
However, if contamination is visible as a faint object in every image acquired, it indicates that the optical parts are compromised, seen in picture below.
Here, compressed air (clean and dry) can be used to remove dust particles from the optical system by blowing air into the cassettes fixture. Do not use compressed air from a single use-can, since these contain chemicals that can reduce optical performance.
Cleaning of NC3000 or NC250
When cleaning the sample tray, great care must be taken against introducing any liquid or dust into the NC-3000/NC250. Any liquid that enters the interior can damage the optical parts and thus compromise the quality of the analyses.
The black surface of the sample tray is anodized aluminum and can therefore be wiped off with a clean, dry and dust free cloth that reduces fiber contamination of the sample tray.
Removal of dust particles from optical windows
Two optical windows are placed in the sample tray. Please ensure that these are always kept clean. In case cleaning of a window is needed then use the Cleaning kit NucleoCounter and follow the instructions below.
Place a clean Alpha Swab at the inner part of the window, and then move it outwards and repeat to cover the complete optical window. The optical window at the Cassette area can be cleaned in the same manner.