How to correlate a cell population in a scatter/histogram plot to those within the fluorescent image (NC-200/250/3000)

Background

Our NucleoCounters, including NC-200, NC-250 and NC-3000, allow users to verify their counting results in the fluorescent images. By doing so, users can trace cells in the fluorescent images back from the scatter/histogram plots and vice versa to identify the validity of the cell counts.

How to correlate a cell population in a scatter/histogram plot to those within the fluorescent image (NC-200/250/3000)

  • Open the raw data of a cell count via plot manager, or right clicking the result in the “Data folder and files” browser and choose “Show Raw Data”.

Double click a scatter/histogram plot, use the “New polygon” / “New Marker” tool on the top bar to select a cell population (shown in pics below as P3/M1).

Right click within the new polygon/marker, click “Add Cell inside Gate to Image Overlay” / “Add Cell inside Marker to Image Overlay”.

Selected cells will show in pink in both scatter plot and fluorescent image. 

Although no changes will show in histogram after adding selected cells to image overlay, cells of selection will still be marked pink in fluorescent image.

Go back to the scatter plot/histogram in the plot manager after verification of selected cells by inspecting in the fluorescent image. Right click within the new polygon/marker, choose “Delete Image Overlay” to cancel the cell selection.

Note: “Add Cell Outside Gate/Marker to Image Overlay” allows to select all cells inside the scatter/histogram plot except those within the new polygon/marker.

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