If a bubble has formed in the cassette, the cell count returned will not be accurate. Another sample will need to be run in order to get an accurate total cell count.
How Detect Bubbles:
- After a sample has been run on your NucleoCounter, click on the “Image Overlay” button in order to see what the instrument has determined as a cell.
The software will put a purple box around each cell
When a bubble has formed and is negatively impacting the accuracy of the data, there will be a large blank portion of the image, like the following examples:
These samples will have to be run again with a new cassette in order to get an accurate cell count.
- Bubbles can be avoided by ensuring:
- There is enough sample volume in the Eppendorf Tube
- The cassette pipette tip is all the way at the bottom of the tube
- You apply smooth and steady pressure to the piston on the cassette
- You allow for a few seconds of equilibration after you have pushed the piston and before you remove the cassette from the sample
- You run the cassette in the NucleoCounter promptly