How to explain the Debris Index parameter on NC-202

Background

The Debris Index is a new parameter added to the NC-202 output data files alongside:

  • Total(cells/ml)
  • Live (cells/ml)
  • Dead (cells/ml)
  • Viability (%)
  • Diameter (µm)
  • Aggregates (%)

The Debris Index is a unit free measurement and it is correlated to the total events counted. When plotting integrated intensity (Intensity 2 in the graphs) of DAPI against AO the size of the event is correlated, meaning that small low intensity events are counted as debris. 

The new parameter was developed in order to be able to follow growth curves and indirectly health of the cells, which is very important when setting up large-scale production using cells.

How to explain the Debris Index parameter on NC-202

Integrated intensity heatmap of single events is displayed below on the right. Intensities in the blue range will be counted as debris.

Below are three examples from NC-202 JAN 2020 DEMO data.

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