NucleoCounter® NC-200™

One Cassette. One Setting. Truly Objective

  • Consistent cell counting and analysis
  • 21 CFR Part 11/GMP-ready
  • Use with Via1-Cassette™ to eliminate human error
  • No calibration required
  • Unlimited software licenses

The NucleoCounter® NC-200™ automated cell counter is a high-precision instrument utilizing state-of-the-art image cytometry. It counts suspension cells, aggregated cells, CAR-T cells, stem cells and cells on microcarriers.

The NucleoCounter® NC-200™ automated cell counter comes with optimized analysis settings for cell count and viability measurements. Along with its user-adaptable protocols and specialized assays, the NC-200™ is an all-in-one solution for cell counting and cell viability determination.

Use the Via1-Cassette™ with this cell counter for automated staining and sample handling. The disposable cassette is volume-calibrated to ensure high-precision counts and cell reproducibility.

Read more about the Cassette technology

This closed cell counting system helps you stay protected when handling samples. No pipettes, no exposure to (toxic) reagents, and minimal clean-up make the NucleoCounter® system a safe option.

Application type(s) Cell count & viability for mammalian cells
Cell type(s) Mammalian cells, including cell lines, leukopaks, PBMCs, whole blood, MSCs, T-cells, aggregated cells, & microcarriers
Software NucleoView™
Consumable(s) Via1-Cassette™
Capacity (Analysis time) 50 seconds (one step), 120 seconds (two steps)
Loading volume 60 μl
Analysis volume 1.4 μl
Optimal range 5×104 to 5×106 cells/ml
Staining dye(s) Acridine orange (AO), DAPI
Excitation 2 LED light sources peaking at 365 nm and 505 nm, dark field light
Emission (nm) A single dual-band emission filter: 410 – 460 nm and 540 – 650 nm
Optics Lens with x1.3 magnification, 1/2.” CMOS camera
Data output Images, tables, histograms, scatter plots
Data export formats .cm, .csv, .pdf, .cmpp, .bmp, .tiff
Product Number
40 × 23 × 25 cm (W × H × D)
4.5 kg (9.9 lb)
Supply voltage
24VDC (100-240V ~ 50-60Hz)
Sampling Devices, Reagents & Test Kits for the NucleoCounter® NC-200™ Automated Cell Counter


Product type Product No. Product name
Sampling Devices 941-0012 Via1-Cassette™
941-0024 Via2-Cassette™
Service Plan 980-5200 Service Plan for NucleoCounter® NC-200™
Software 950-0201 NucleoView™
Reagents  910-0003 Reagent A100 Lysis Buffer, 500 ml
910-0002 Reagent B Stabilizing Buffer, 500 ml
910-3010 Solution 10 Lysis Buffer, 100 ml
910-3017 Solution 17 Blood Lysis Buffer, 25 ml
Accessories 930-0005 Power Supply NucleoCounter® NC-200™, NC-250™, SCC-400™
929-0012 Laptop Stand II
929-0010 Short LID Yellow

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