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Adhering to 21 CFR part 11 is essential in cGMP environments
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Please note:
The NucleoCounter® NC-200™, NC-250™ and NC-3000™ instruments will be discontinued and will only be available for ordering until April 2027.
The designated replacements are: NucleoCounter® NC-203™, XcytoMatic® 30 and XcytoMatic® 40.
NucleoCounter® NC-200™
One Cassette. One Setting. Truly Objective
- Consistent cell counting and analysis
- 21 CFR Part 11/GMP-ready
- Use with Via1-Cassette™ to eliminate human error
- No calibration required
- Unlimited software licenses
The NucleoCounter® NC-200™ automated cell counter is a high-precision instrument utilizing state-of-the-art image cytometry. It counts suspension cells, aggregated cells, CAR-T cells, stem cells and cells on microcarriers.
The NucleoCounter® NC-200™ automated cell counter comes with optimized analysis settings for cell count and viability measurements. Along with its user-adaptable protocols and specialized assays, the NC-200™ is an all-in-one solution for cell counting and cell viability determination.
Use the Via1-Cassette™ with this cell counter for automated staining and sample handling. The disposable cassette is volume-calibrated to ensure high-precision counts and cell reproducibility.
Read more about the Cassette technology
This closed cell counting system helps you stay protected when handling samples. No pipettes, no exposure to (toxic) reagents, and minimal clean-up make the NucleoCounter® system a safe option.
| Application type(s) | Cell count & viability for mammalian cells |
| Cell type(s) | Mammalian cells, including cell lines, leukopaks, PBMCs, whole blood, MSCs, T-cells, aggregated cells, & microcarriers |
| Software | NucleoView™ |
| Consumable(s) | Via1-Cassette™ |
| Capacity (Analysis time) | 50 seconds (one step), 120 seconds (two steps) |
| Loading volume | 60 μl |
| Analysis volume | 1.4 μl |
| Optimal range | 5×104 to 5×106 cells/ml |
| Staining dye(s) | Acridine orange (AO), DAPI |
| Excitation | 2 LED light sources peaking at 365 nm and 505 nm, dark field light |
| Emission (nm) | A single dual-band emission filter: 410 – 460 nm and 540 – 650 nm |
| Optics | Lens with x1.3 magnification, 1/2.” CMOS camera |
| Data output | Images, tables, histograms, scatter plots |
| Data export formats | .cm, .csv, .pdf, .cmpp, .bmp, .tiff |
| Product Number |
900-0201 |
| Size |
40 × 23 × 25 cm (W × H × D) |
| Weight |
4.5 kg (9.9 lb) |
| Supply voltage |
24VDC (100-240V ~ 50-60Hz) |
Sampling Devices, Reagents & Test Kits for the NucleoCounter® NC-200™ Automated Cell Counter
| Product type | Product No. | Product name |
| Sampling Devices | 941-0012 | Via1-Cassette™ |
| 941-0024 | Via2-Cassette™ | |
| Service Plan | 980-5200 | Service Plan for NucleoCounter® NC-200™ |
| Software | 950-0201 | NucleoView™ |
| Reagents | 910-0003 | Reagent A100 Lysis Buffer, 500 ml |
| 910-0002 | Reagent B Stabilizing Buffer, 500 ml | |
| 910-3010 | Solution 10 Lysis Buffer, 100 ml | |
| 910-3017 | Solution 17 Blood Lysis Buffer, 25 ml | |
| Accessories | 930-0005 | Power Supply NucleoCounter® NC-200™, NC-250™, SCC-400™ |
| 929-0012 | Laptop Stand II | |
| 929-0010 | Short LID Yellow |
| Title | Categories | Download |
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