NucleoCounter® NC-250™

Automated Cell Analyzer

  • Fast, high-precision cell counts
  • Analyzes total cell count and viability for 8 samples in 3 minutes
  • Easy to operate
  • Excellent data reproducibility
  • No calibration required
  • 21 CFR Part 11/GMP-ready

The NucleoCounter® NC-250™ is an automated cell analyzer, which can characterize cell properties using fluorescence imaging. The NC-250™ offers a wide range of optimized protocols to meet the needs of all mammalian cell types grown under different conditions.

Through unique assays, cell count can be performed even on cells growing on microcarriers and in sphere cultures. This benchtop image cytometer ensures high-precision and does not require calibration.

With a combination of simplicity and innovation, counting and viability determination of mammalian cells has never been easier. Simply mix a small volume of cell suspension with fluorescent dyes, load, and run. Within three minutes you will have eight highly precise viability and cell count analyses at hand.

For cells with low concentration, or to achieve even higher precision, users can take advantage of the NC-Slide A2™ with a 30 µl volume capacity.

The NucleoCounter® NC-250™ automated cell analyzer performs high-speed cell count and viability determination and offers a unique 5-minute Cell Cycle Assay and a 1-minute Apoptosis Assay for up to eight samples at the time.

The intuitive software has unlimited licenses and produces on-screen data analysis and automated PDF reports.

Application type(s) Cell count & viability, Cell cycle, Vitality
Cell type(s) Mammalian
Software NucleoView™
Consumable(s) NC-Slide A2™ and NC-Slide A8™
Capacity (Analysis time) Variable, depending on consumable and assay type
Loading volume 30 μl (NC-Slide A2™), 10 μl (NC-Slide A8™)
Analysis volume 0.8 – 16 μl sample (NC-Slide A2™, NC-Slide A8™)
Optimal range 5×104 to 5×106 cells/ml
Staining dye(s) Acridine orange (AO), DAPI, VitaBright-48™, Propidium iodide (PI), User-defined
Excitation Two LED light sources with peaks at 365 nm and 500 nm + one dark field white LED
Emission (nm) A single dual-band emission filter: 410 – 450 nm and 520 – 595 nm
Optics Lens with ×1.75 magnification, 1/2.5” CMOS camera
Data output Images, tables, histograms, scatter plots
Data export formats .cm, .csv, .pdf, .cmpp, .bmp, .tiff, .acs, .fcs
Product Number
19 × 29 × 32 cm (8 × 12 × 13”) (W × H × D)
9 kg (20 lbs)
Supply voltage
24VDC (100–240 V ~ 50–60 Hz)