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NucleoCounter® NC-3000™

Advanced Image Cytometer

 

  • A variety of fixed and build-your-own assays
  • High-speed cell counting and advanced data analysis
  • No warm-up or calibration required
  • No liquid waste produced
  • 21 CFR Part 11/GMP-ready software
NucleoCounter® NC-3000™ front
NucleoCounter® NC-3000™ right
NucleoCounter® NC-3000™ left
NucleoCounter® NC-3000™ front
NucleoCounter® NC-3000™ right
NucleoCounter® NC-3000™ left
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The NucleoCounter® NC-3000™ advanced image cytometer utilizes fluorescence imaging to characterize cell properties. The instrument performs high-speed plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute cell cycle assay, a selection of apoptosis assays and the standard cell count and viability assay.

With the FlexiCyte™ license, you can create protocols with options for light source, emission filters and other detailed settings. The accompanying NucleoView™ software is ideal for intuitive data analysis and has unlimited licenses. You can view data in plot graphs and interlinked images, which can be saved for later on-demand analysis.

This advanced image cytometer ensures flow-like analysis with high reproducibility, operates with low sample volumes, and is calibration-free.

Used with the disposable Via1-Cassette™, this closed cell counting system helps you stay protected when handling samples. No pipettes, no exposure to (toxic) reagents, and minimal clean-up make the NucleoCounter® NC-3000™ a safe option.

Read more about the Cassette technology

 

  • Assays: 5-minute cell cycle analysis, 1-minute apoptosis assay, 1-minute GFP assay
  • Exports to data analysis tools, such as FCS Express and FlowJo
  • PDF reports
  • Unlimited software installs and free updates
  • 5-channel open platform
Application type(s) Fixed assays: Cell count & viability, Cell cycle, Mitochondrial potential, Vitality, Caspase, Annexin V, Transfection efficiency, and DNA fragmentation assays. User-adapted protocols with the Flexicyte™ module
Cell type(s) Mammalian, yeast, insect, avian
Software NucleoView™
Consumable(s) Via1-Cassette™, NC-Slide A2™ and NC-Slide A8™
Capacity (Analysis time) Variable, depending on consumable and assay type
Loading volume 60 μl (Via1-Cassette™), 30 μl (NC-Slide A2™), 10 μl (NC-Slide A8™)
Analysis volume 3.2 μl sample (Via1-Cassette™), 0.8 – 16 μl sample (NC-Slide A2™, NC-Slide A8™)
Optimal range 5×104 to 5×106 cells/ml
Staining dye(s) Hoechst 33342, JC-1, VitaBright-43™, VitaBright-48™, Acridine orange (AO), DAPI, Propidium iodide (PI), User-defined
Excitation Seven LED light sources with peaks at 365 nm, 405 nm, 475 nm, 530 nm, 630 nm + 2 white LEDs
Emission (nm) Nine interchangeable emission filters: 430/20, 470/55, 475/15,
530/15, 560/35, 580/25, 675/75, 630 LP, 740/60
Optics Lens with x2 magnification, 1/2″ CCD camera with 1392×1040 pixels
Data output Images, tables, histograms, scatter plots
Data export formats .cm, .csv, .pdf, .cmpp, .bmp, .tiff, .acs, .fcs
Product Number
 970-3002 (Fixed Analytical Assays); 970-3003 (FlexiCyte™)
Size
 29 x 29 x 31 cm (W x H x D)
Weight
 14 kg (30.9 lb)
Supply voltage
 24VDC (100–240 V ~ 50–60 Hz)

Sampling Devices, Reagents & Test Kits for the NucleoCounter® NC-3000™ Advanced Cell Analyzer.

 

Product type Product No. Product name
Sampling Devices 941-0012 Via1-Cassette™
941-0016 PI-Cassette™
942-0001 NC-Slide A2™
942-0003 NC-Slide A8™
Software NucleoView™
Solutions 910-0003 Reagent A100 Lysis Buffer, 500 ml
910-0002 Reagent B Stabilizing Buffer, 500 ml
910-3002 Solution 2 Propidium Iodide Staining Reagent, 25 ml
910-3003 Solution 3 DAPI Staining Reagent, 25 ml
910-3005 Solution 5 VB-48™ PI – AO Staining Reagent, 1 ml
910-3007 Solution 7 JC-1 Staining Reagent, 1ml
910-3008 Solution 8 DAPI PBS Staining Reagent, 25ml
910-3010 Solution 10 Lysis Buffer, 100 ml
910-3011 Solution 11 Stabilization Buffer, 100 ml
910-3012 Solution 12 DAPI Staining Reagent, 1 ml
910-3013 Solution 13 AO – DAPI Staining Reagent, 1ml
910-3015 Solution 15 Hoechst 3342 Staining Reagent, 1ml
910-3016 Solution 16 Propidium Iodide Staining Reagent, 1ml
910-3017 Solution 17 Blood Lysis Buffer, 25 ml
910-3018 Solution 18 AO•DAPI Staining Reagent, 1 ml
910-3019 Solution 19 VitaBright-43™, 100 µl
910-3020 Solution 20 VitaBright-48™ Staining Reagent, 100 µl
Accessories 930-0004 Power Supply NucleoCounter® NC-3000™
911-0013 NucleoCounter® Cleaning Kit
TitleCategoriesDownload
A comparison of DNA quantitation by image and flow cytometryScientific Poster
A decrease in cellular thiols caused by drug-inducedScientific Poster
A novel rapid apoptosis assay based on thiol redox statusScientific Poster
A Novel Scalable Production Platform for Lentiviral Vectors Based on Human Suspension Cell LinesScientific Poster
A simple and rapid method for DNA content measurementsScientific Poster
App Note Annexin V Assay NC-3000™Application Note
App Note Caspase 3, 7, 8/9 (FLICA) Assay NC-3000™Application Note
App Note Cell Count & Viability Aggregated Cells Via1-Cassette™ NC-3000™Application Note
App Note Cell Count Aggregated Cells NC-Slides NC-3000™Application Note
App Note Cell Count Aggregated Cells NC-Slides™ Reagent A100 and B NC-3000™Application Note
App Note Cell Count Aggregated Cells Via1-Cassette™ Reagent A100 and B NC-3000™Application Note
App Note Cell Count and Viability Aggregated Cells NC-Slide A2™ NC-3000™Application Note
App Note Cell Count and Viability Aggregated Cells Via1-Cassette™ NC-3000™Application Note
App Note Cell Count and Viability Insect Cells NC-Slides A8™ NC-3000™Application Note
App Note Cell Count and Viability Insect Cells Via1-Cassette™ NC-3000™Application Note
App Note Cell Count and Viability Mammalian Cells NC-slides A2™ Reagent A100 and B NC-3000™Application Note
App Note Cell Count and Viability Mammalian Cells NC-Slides A8™ NC-3000™Application Note
App Note Cell Count and Viability Mammalian Cells NC-Slides Method 2 NC-3000™Application Note
App Note Cell Count and Viability Mammalian Cells PI-Cassette™ NC-3000™Application Note
App Note Cell Count and Viability Mammalian Cells Via1-Cassette™ NC-3000™Application Note
App Note Cell Count and Viability Mammalian Cells Via1-Cassette™ NC-3000™Application Note
App Note Cell Count and Viability Mammalian Cells Via1-Cassette™ Reagent A100 and B NC-3000™Application Note
App Note Cell Count and Viability Purified Leukocyte NC-3000™Application Note
App Note Cell Count and Viability Whole Blood NC-Slides™ NC-3000™Application Note
App Note Cell Count and Viability Yeast Cells PI-Cassette™ NC-3000™Application Note
App Note Cell Count Mammalian Cells PI-Cassette™ NC-3000™Application Note
App Note Cell Count Yeast Cells PI-Cassette™ NC-3000™Application Note
App Note Cell Cycle Fixed Cells NC-3000™Application Note
App Note DNA Fragmentation NC-3000™Application Note
App Note FlexiCyte™ Assay NC-3000™Application Note
App Note GFP Transfection Hoechst and PI-Cassette™ NC-3000™Application Note
App Note Mitochondrial Potential NC-3000™Application Note
App Note Two Step Cell Cycle NC-3000™Application Note
App Note Vitality Assay NC-3000™Application Note
Cell count determination of aggregated HEK293 suspension cells and Vero cells on microcarriersScientific Poster
Delineating the Critical Process Parameters for Cell Therapy CryopreservationScientific Poster
NC-3000™ Product VideoVideo
NucleoCounter® NC-3000™ BrochureBrochure
NucleoCounter® NC-3000™ Validation PlanBrochure
Quantitative assesment of apoptotic events using image and flow cytometryScientific Poster
Quick Guide: NucleoCounter® NC-3000™User Guide
Software User Guide: NucleoView™ NC-3000™User Guide
Tech Note: Cleaning Guide for NucleoCounter® NC-3000™Technical Note
Tech Note: LED Life Time Consideration for NucleoCounter® NC-3000™Technical Note
Tech Note: Variations and Statistics for NucleoCounter® NC-3000™Technical Note
The Effect of Cryomedia Selection and Transient Warming Events on Post-Cryopreservation Human MSC FunctionScientific Poster
Transition to GMP, chemically-defined, xeno-free cryopreservation media increases post-thaw functionality of clinically relevant cell banksScientific Poster
User Guide: NucleoCounter® NC-3000™ Advanced Image CytometerUser Guide
White Paper: NucleoView™ NC-3000™ Software and CFR 21 Part 11White Paper