NucleoCounter® SP-100™

Automated Sperm Cell Counter

  • No interference from gel particles or debris in semen
  • No interference from egg yolk or milk
  • Measures % viability
  • Excellent reproducibility
  • No calibration required

The NucleoCounter® SP-100™ automated sperm cell counter is ideal for use in animal reproduction. The instrument combines ease of use with effectively determining total sperm concentration and sperm viability from ejaculates or extended doses.

Even users with limited training in laboratory work can operate the SP-100™. Its compact size fits perfectly into any research, quality control or production site.

This automated sperm cell counter has a virtually unlimited measurement range due to dilution prior to the analysis.

SemenView™ is the accompanying software for the NucleoCounter® SP-100™ automated sperm counter. The optional software can be used to save data or create a report—the instrument can run without the software, as it has a digital display of the cell count.

Read more about the Cassette technology

Application type(s) Cell count & viability analysis
Cell type(s) Most semen samples, including bull, boar, equine, ram, canine, monkey, fish
Software SemenView™ for documentation and
presentation (included)
Consumable(s) SP1-Cassette™, Reagent S100 Lysis Buffer (500ml),
Reagent S100 Lysis Buffer (5l), PBS Phosphate Buffered Saline (500ml)
Capacity (Analysis time) 30 seconds (total cell count); 80 seconds (non-viable cell count)
Loading volume 60 μl
Analysis volume 1.4 μl
Optimal range 5×104 to 5×106 cells/ml
Staining dye(s) Propidium iodide (PI)
Optics CCD camera
Data output Data numbers
Data export formats .txt, .xls
Product Number
40 × 23 × 25 cm (W x H x D)
3kg (6.8 lb)
Supply voltage 24VDC (100-240V ~ 50-60Hz)
Sampling Devices, Reagents & Test Kits for the NucleoCounter® SP-100™ Sperm Cell Counter.


Product type Product No. Product name
Sampling Devices 941-0006 SP1-Cassette™
Software 950-0100 SemenView™
Solutions 910-0101 Reagent S100 Lysis Buffer, 500 ml
910-0100 Reagent S100 Lysis Buffer, 5 l
910-0008 PBS Phosphate Buffered Saline, 500 ml
910-0009 PBS Phosphate Buffered Saline, 5 l
Accessories 930-0002 Power Supply: NucleoCounter® NC-100™, YC-100™, SP-100™, SCC-100™
911-0003 Dispenser 1-10 ml
929-0007 Short LID Grey
929-0003 Container Stand for 5 l Reagent
931-0001 USB Cable 2.0m
911-0004 Sample Cup 20 mL, 1000 pcs.
929-0001 Bottle Stand
939-0012 LTS Paper for PCNEOS-S2BN
911-0013 NucleoCounter® Cleaning Kit