NucleoCounter® NC-3000™

Advanced Image Cytometer

  • A variety of fixed and build-your-own assays
  • High-speed cell counting and advanced data analysis
  • No warm-up or calibration required
  • No liquid waste produced
  • 21 CFR Part 11/GMP-ready software


The NucleoCounter® NC-3000™ advanced image cytometer utilizes fluorescence imaging to characterize cell properties. The instrument performs high-speed plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute cell cycle assay, a selection of apoptosis assays and the standard cell count and viability assay.

With the FlexiCyte™ license, you can create protocols with options for light source, emission filters and other detailed settings. The accompanying NucleoView™ software is ideal for intuitive data analysis and has unlimited licenses. You can view data in plot graphs and interlinked images, which can be saved for later on-demand analysis.

This advanced image cytometer ensures flow-like analysis with high reproducibility, operates with low sample volumes, and is calibration-free.

Used with the disposable Via1-Cassette™, this closed cell counting system helps you stay protected when handling samples. No pipettes, no exposure to (toxic) reagents, and minimal clean-up make the NucleoCounter® NC-3000™ a safe option.

  • Assays: 5-minute cell cycle analysis, 1-minute apoptosis assay, 1-minute GFP assay
  • Exports to data analysis tools, such as FCS Express and FlowJo
  • PDF reports
  • Unlimited software installs and free updates
  • 5-channel open platform


NC-3000™ Product Video


Application type(s):

Fixed assays: Cell count & viability, Cell cycle, Mitochondrial potential, Vitality, Caspase, Annexin V, Transfection efficiency, DNA fragmentation assays. User-adapted protocols with the Flexicyte™ module

Cell type(s):

Mammalian, yeast, insect, avian

Optimal range:

5×104 to 5×106 cells/ml (for counting)

Loading volume:

60 μl (Via1-Cassette™), 30 μl (NC-Slide A2™), 10 μl (NC-Slide A8™)

Analysis time:

Variable, depending on consumable and assay type

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