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Publications involving our NucleoCounter® instruments.
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2023
Odd- and even-numbered medium-chained fatty acids protect against glutathione depletion in very long-chain acyl-CoA dehydrogenase deficiency
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Lund M, Heaton R, Hargreaves IP, et al. ,
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Biochim Biophys Acta Mol Cell Biol Lipids ,
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2023,
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1868(2):159248
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Primary Cells
- Assay: Cell Vitality Assay
- Research area: Cell Biology
Recent trials have reported the ability of triheptanoin to improve clinical outcomes for the severe symptoms associated with long-chain fatty acid oxidation disorders, including very long-chain acyl-CoA dehydrogenase (VLCAD) deficiency. However, the milder myopathic symptoms are still challenging to treat...
View PublicationThe remediation of di-(2-ethylhexyl) phthalate-contaminated sediments by water hyacinth biochar activation of calcium peroxide and its effect on cytotoxicity
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Hung CM, Huang CP, Hsieh SL, et al. ,
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Environ Res ,
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2023,
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216(Pt 3):114656
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay
- Research area: Oncology
The presence of di-(2-ethylhexyl) phthalate (DEHP) in the aquatic systems, specifically marine sediments has attracted considerable attention worldwide, as it enters the food chain and adversely affects the aquatic environment and subsequently human health. This study reports an efficient carbocatalytic...
View PublicationCardioprotective Effect of Centaurea castriferrei Borbás & Waisb Extract against Doxorubicin-Induced Cardiotoxicity in H9c2 Cells
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Humeniuk E, Adamczuk G, Kubik J, et al. ,
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Molecules ,
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2023,
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28(1):420
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Oncology
Doxorubicin (DOX) is one of the most used chemotherapeutic agents in the treatment of various types of cancer. However, a continual problem that is associated with its application in therapeutic regimens is the development of dose-dependent cardiotoxicity. The progression of...
View PublicationEvaluation of developmental toxicity in zebrafish embryos and antiproliferative potential against human tumor cell lines of new derivatives containing 4-nitrophenyl group
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Szopa A, Herbet M, Pachuta-Stec A, et al. ,
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Toxicol Appl Pharmacol ,
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2023,
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458:116325
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay
- Research area: Oncology
The aim of the studies was to evaluate the antiproliferative potential against human tumor cell lines of newly synthetized derivatives containing 4-nitrophenyl group, as well as its impact on developmental toxicity in zebrafish model. We selected 1-(4-nitrobenzoyl)-4-ethylsemicarbazide (APS-1) and 1-[(4-nitrophenyl)acetyl]-4-hexyl-thiosemicarbazide...
View PublicationTROP2 expression and SN38 antitumor activity in malignant pleural mesothelioma cells provide a rationale for antibody-drug conjugate therapy
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Hegedüs L, Okumus Ö, Mairinger F, et al. ,
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Lung Cancer ,
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2023,
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178:237-246
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay
- Research area: Oncology
Objectives: Malignant pleural mesothelioma (MPM) is an aggressive cancer which at large is not amenable to curative surgery. Despite the recent approval of immune checkpoint inhibitor therapy, the response rates and survival following systemic therapy is still limited. Sacituzumab govitecan...
View PublicationDesign and optimization various formulations of PEGylated niosomal nanoparticles loaded with phytochemical agents: potential anti-cancer effects against human lung cancer cells
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Shahbazi R, Jafari-Gharabaghlou D, Mirjafary Z, et al. ,
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Pharmacol Rep ,
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2023,
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75(2):442-455
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Background: Phytochemicals and their derivatives are good options to improve treatment efficiency in cancer patients. Artemisinin (ART) and metformin (MET) are widely used phytochemicals to treat various types of cancers. However, their application because of their dose-dependent side effects, and...
View PublicationEvaluation of Possible Neobavaisoflavone Chemosensitizing Properties towards Doxorubicin and Etoposide in SW1783 Anaplastic Astrocytoma Cells
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Maszczyk M, Banach K, Rok J, et al. ,
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Cells ,
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2023,
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12(4):593
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, Mitochondrial Potential Assay
- Research area: Oncology
Flavonoids exert many beneficial properties, such as anticancer activity. They were found to have chemopreventive effects hindering carcinogenesis, and also being able to affect processes important for cancer cell pathophysiology inhibiting its growth or promoting cell death. There are also...
View PublicationBioactive self-healing hydrogel based on tannic acid modified gold nano-crosslinker as an injectable brain implant for treating Parkinson’s disease
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Xu J, Chen TY, Tai CH, Hsu SH ,
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Biomater Res ,
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2023,
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27(1):8
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Neural Cells
- Assay: Cell Vitality Assay
- Research area: Neurobiology
Background: Parkinson’s disease (PD) is one of the most common long-term neurodegenerative diseases. Current treatments for PD are mostly based on surgery and medication because of the limitation and challenges in selecting proper biomaterials. In this study, an injectable bioactive...
View PublicationMaternal phthalate exposure during pregnancy and testis function of young adult sons
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Henriksen LS, Frederiksen H, Jørgensen N, et al. ,
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Sci Total Environ ,
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2023,
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871:161914
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Background: Phthalate exposure during fetal life may disrupt testicular development. Congruent with this, studies have found shorter anogenital distance, reduced penile size and altered hormone levels in infant boys whose mothers were exposed to higher levels of some phthalates during...
View PublicationThe Assessment of the Phototoxic Action of Chlortetracycline and Doxycycline as a Potential Treatment of Melanotic Melanoma-Biochemical and Molecular Studies on COLO 829 and G-361 Cell Lines
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Rok J, Rzepka Z, Banach K, et al. ,
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Int J Mol Sci ,
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2023,
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24(3):2353
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Caspase 3/7 or 8 or 9 Assay, Cell Count & Viability Assay, Cell Vitality Assay, DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Oncology
Melanoma is still one of the most dangerous cancers. New methods of treatment are sought due to its high aggressiveness and the relatively low effectiveness of therapies. Tetracyclines are drugs exhibiting anticancer activity. Previous studies have also shown their activity...
View PublicationSeed-derived peptide lunasin suppressed breast cancer cell growth by regulating inflammatory mediators, aromatase, and estrogen receptors
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Hsieh CC, Wu CH, Peng SH, Chang CH ,
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Food Nutr Res ,
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2023,
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67
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Vitality Assay
- Research area: Oncology
Background: Breast cancer is one of the most prevalent cancers in women. Its pathology comprises tumor cells and nearby stromal cells, accompanied by cytokines and stimulated molecules, resulting in a favorable microenvironment for tumor progression. Lunasin is a seed peptide...
View PublicationEpithelial disruption drives mesendoderm differentiation in human pluripotent stem cells by enabling TGF-β protein sensing
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Legier T, Rattier D, Llewellyn J, et al. ,
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Nat Commun ,
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2023,
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14(1):349
- Instrument : NucleoCounter® NC-3000™
- Cell Type: iPSCs & ESCs
- Assay: Cell Cycle Assay
- Research area: Stem Cell Research
The processes of primitive streak formation and fate specification in the mammalian epiblast rely on complex interactions between morphogens and tissue organization. Little is known about how these instructive cues functionally interact to regulate gastrulation. We interrogated the interplay between...
View PublicationInvolvement of Mitochondrial Dysfunction in FOXG1 Syndrome
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Bjerregaard VA, Levy AM, Batz MS, et al. ,
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Genes (Basel) ,
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2023,
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14(2):246
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay, Mitochondrial Potential Assay
- Research area: Neurobiology
FOXG1 (Forkhead box g1) syndrome is a neurodevelopmental disorder caused by a defective transcription factor, FOXG1, important for normal brain development and function. As FOXG1 syndrome and mitochondrial disorders have shared symptoms and FOXG1 regulates mitochondrial function, we investigated whether...
View PublicationDesign, Synthesis and Biological Evaluation of Quinoline-8-Sulfonamides as Inhibitors of the Tumor Cell-Specific M2 Isoform of Pyruvate Kinase: Preliminary Study
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Marciniec K, Rzepka Z, Chrobak E, et al. ,
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Molecules ,
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2023,
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28(6):2509
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, DNA Fragmentation Assay
- Research area: Oncology
Cancer cells need to carefully regulate their metabolism to keep them growing and dividing under the influence of different nutrients and oxygen levels. Muscle isoform 2 of pyruvate kinase (PKM2) is a key glycolytic enzyme involved in the generation of...
View PublicationAdipogenic and SWAT cells separate from a common progenitor in human brown and white adipose depots
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Palani NP, Horvath C, Timshel PN, et al. ,
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Nat Metab ,
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2023,
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5(6):996-1013
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Primary Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Adipocyte function is a major determinant of metabolic disease, warranting investigations of regulating mechanisms. We show at single-cell resolution that progenitor cells from four human brown and white adipose depots separate into two main cell fates, an adipogenic and a...
View PublicationCytotoxic properties of unfractionated and fractionated bromelain alone or in combination with chemotherapeutic agents in colorectal cancer cells
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Tsai KY, Wei PL, Azarkan M, et al. ,
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PLoS One ,
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2023,
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18(6):e0285970
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
“Background: Colorectal cancer (CRC) is one of the most lethal cancers worldwide. Long-term survival is not achieved in metastatic CRC despite the current multidisciplinary therapies. Bromelain, a compound extracted from the pineapple plant, has multiple functions and anticancer properties. Previously,...
View PublicationUnderstanding the impact of bioactive coating materials for human mesenchymal stromal cells and implications for manufacturing
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Silva Couto P, Molina SA, O'Sullivan D, et al. ,
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Biotechnol Lett ,
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2023,
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45(8):1013-1027
- Instrument : NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Bioactive materials interact with cells and modulate their characteristics which enable the generation of cell-based products with desired specifications. However, their evaluation and impact are often overlooked when establishing a cell therapy manufacturing process. In this study, we investigated the...
View PublicationDownstream process design for Gag HIV‐1 based virus‐like particles
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Lorenzo E, Miranda L, Gòdia F, Cervera L ,
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Biotechnol Bioeng ,
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2023,
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- Instrument : NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Virus Production
Virus-like particles-based vaccines have been gaining interest in recent years. The manufacturing of these particles includes their production by cell culture followed by their purification to meet the requirements of its final use. The presence of host cell extracellular vesicles...
View PublicationGuanidines: Synthesis of Novel Histamine H3R Antagonists with Additional Breast Anticancer Activity and Cholinesterases Inhibitory Effect
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Staszewski M, Iwan M, Werner T, et al. ,
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Pharmaceuticals ,
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2023,
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16(5):675
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Oncology
This study examines the properties of novel guanidines, designed and synthesized as histamine H3R antagonists/inverse agonists with additional pharmacological targets. We evaluated their potential against two targets viz., inhibition of MDA-MB-231, and MCF-7 breast cancer cells viability and inhibition of...
View PublicationStaphylococcus microti Strains Isolated from an Italian Mediterranean Buffalo Herd
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Ambrosio M, Nocera FP, Garofalo F, et al. ,
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Animals (Basel) ,
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2023,
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13(1):182
- Instrument : NucleoCounter® SCC-100™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Dairy (SCC)
S. microti is a new species among non-aureus staphylococci (NAS) frequently found in bovine milk samples and associated with subclinical mastitis (SCM). The aim of this study was to analyze the presence of S. microti in 200 composite milk samples...
View PublicationA self-healing hydrogel and injectable cryogel of gelatin methacryloyl-polyurethane double network for 3D printing
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Cheng QP, Hsu SH ,
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Acta Biomater ,
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2023,
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164:124-138
- Instrument : NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Vitality Assay
- Research area: Stem Cell Research
Three-dimensional (3D) printing of soft biomaterials facilitates the progress of personalized medicine. The development for different forms of 3D-printable biomaterials can promotes the potential manufacturing for artificial organs and provides biomaterials with the required properties. In this study, gelatin methacryloyl...
View PublicationmTOR inhibition amplifies the anti-lymphoma effect of PI3Kβ/δ blockage in diffuse large B-cell lymphoma
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Xu W, Berning P, Erdmann T, et al. ,
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Leukemia ,
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2023,
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37(1):178-189
- Instrument : NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Diffuse large B-cell lymphoma (DLBCL) is an aggressive disease that exhibits constitutive activation of phosphoinositide 3-kinase (PI3K) driven by chronic B-cell receptor signaling or PTEN deficiency. Since pan-PI3K inhibitors cause severe side effects, we investigated the anti-lymphoma efficacy of the...
View PublicationEx vivo prime editing of patient haematopoietic stem cells rescues sickle-cell disease phenotypes after engraftment in mice
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Everette KA, Newby GA, Levine RM, et al. ,
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Nat Biomed Eng ,
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2023,
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7(5):616-628
- Instrument : NucleoCounter® NC-3000™
- Cell Type: HSCs
- Assay: Cell Count & Viability Assay
- Research area: Gene Therapy
Sickle-cell disease (SCD) is caused by an A·T-to-T·A transversion mutation in the β-globin gene (HBB). Here we show that prime editing can correct the SCD allele (HBBS) to wild type (HBBA) at frequencies of 15%-41% in haematopoietic stem and progenitor...
View PublicationComparative study of lung toxicity of E-cigarette ingredients to investigate E-cigarette or vaping product associated lung injury
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Yoon, SH, Song MK, et al. ,
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Journal of Hazardous Materials ,
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2023,
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445.130.454
- Instrument : NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
No comparative study has yet been performed on the respiratory effects of individual E-cigarette ingredients. Here, lung toxicity of individual ingredients of E-cigarette products containing nicotine or tetrahydrocannabinol was investigated. Mice were intratracheally administered propylene glycol (PG), vegetable glycerin (VG),...
View PublicationImproving the response to oxaliplatin by targeting chemotherapy-induced CLDN1 in resistant metastatic colorectal cancer cells
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Cherradi S, Garambois V, Marines J, et al. ,
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Cell Biosci ,
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2023,
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13(1):72
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay
- Research area: Oncology
Background: Tumor resistance is a frequent cause of therapy failure and remains a major challenge for the long-term management of colorectal cancer (CRC). The aim of this study was to determine the implication of the tight junctional protein claudin 1...
View PublicationEvaluation of a new complement-dependent lymphocytotoxicity cross match method using an automated cell counter, the NucleoCounter® NC-3000™
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Pedini P, Baudey JB, Basire A, et al. ,
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HLA ,
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2023,
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101(6):647-659
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based immunotherapy
Complement-dependent lymphocytotoxicity cross match (CDC-XM) is the ultimate test of donor/recipient compatibility prior to organ transplantation. This test is based on cell viability, evaluated under fluorescence microscopy by an operator after proper staining. The determination of the positivity threshold may...
View Publication3D Spheroid Cultivation Alters the Extent and Progression of Osteogenic Differentiation of Mesenchymal Stem/Stromal Cells Compared to 2D Cultivation
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Wolff A, Frank M, Staehlke S, et al. ,
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Biomedicines ,
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2023,
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11(4):1049
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Primary Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Mesenchymal stem/stromal cells (MSC) are capable of progenitor cell fraction renewal or tissue-specific differentiation. These properties are maintained during in vitro cultivation, making them an interesting model system for testing biological and pharmacological compounds. Cell cultivation in 2D is commonly...
View PublicationProtocol development to further differentiate and transition stem cell-derived pancreatic progenitors from a monolayer into endocrine cells in suspension culture
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Braam MJS, Zhao J, Liang S, et al. ,
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Sci Rep ,
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2023,
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13(1):8877
- Instrument : NucleoCounter® NC-3000™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The generation of functional β-cells from human pluripotent stem cells (hPSCs) for cell replacement therapy and disease modeling of diabetes is being investigated by many groups. We have developed a protocol to harvest and aggregate hPSC-derived pancreatic progenitors generated using...
View PublicationIntegrin α10β1-selected mesenchymal stem cells reduced hypercoagulopathy in a porcine model of acute respiratory distress syndrome
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Edström D, Niroomand A, Stenlo M, et al. ,
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Respir Res ,
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2023,
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24(1):145
- Instrument : NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
Mesenchymal stem cells (MSCs) have been studied for their potential benefits in treating acute respiratory distress syndrome (ARDS) and have reported mild effects when trialed within human clinical trials. MSCs have been investigated in preclinical models with efficacy when administered...
View PublicationMetformin induces autophagy of cisplatin-resistant human gastric cancer cells in addition to apoptosis
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Fang CW, Yang JS, Chiang JH, et al. ,
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BioMedicine ,
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2023,
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13(2):2
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Caspase 3/7 or 8 or 9 Assay, Cell Count & Viability Assay
- Research area: Oncology
Metformin has been used to treat cases of type 2 diabetes mellitus, and mounting studies have shown that metformin can act alone or in synergy with other anticancer agents to achieve anti-cancer efficacies on various types of tumors. However, the...
View PublicationHdac1-deficiency affects the cell cycle axis Cdc25-Cdk1 causing impaired G2/M phase progression and reduced cardiomyocyte proliferation in zebrafish
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Boos A, Gahr BM, Park DD, et al. ,
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Biochem Biophys Res Commun ,
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2023,
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665:98-106
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Cycle Assay
- Research area: Cardiology
Zebrafish have the ability to fully regenerate their hearts after injury since cardiomyocytes subsequently dedifferentiate, re-enter cell cycle, and proliferate to replace damaged myocardial tissue. Recent research identified the reactivation of dormant developmental pathways during cardiac regeneration in adult zebrafish,...
View PublicationLysis and phenotypic modulation of mesenchymal stromal cells upon blood contact triggers anti-inflammatory skewing of the peripheral innate immune repertoire
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Davies LC, Queckbörner S, Jylhä CE, et al. ,
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Cytotherapy ,
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2023,
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S1465-3249(23)00954-4
- Instrument : NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Annexin V Assay, Cell Count & Viability Assay, Mitochondrial Potential Assay
- Research area: Stem Cell Research
Background aims: Mesenchymal stromal cells (MSCs) are used to treat immune-related disorders, including graft-versus-host disease. Upon intravenous infusion, MSCs trigger the instant blood-mediated inflammatory response, resulting in activation of both complement and coagulation cascades, and are rapidly cleared from circulation....
View Publication2,3,5,4′-Tetrahydroxystilbene (TG1), a Novel Compound Derived from 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside (THSG), Inhibits Colorectal Cancer Progression by Inducing Ferroptosis, Apoptosis, and Autophagy
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Tsai KY, Wei PL, Lee CC, et al. ,
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Biomedicines ,
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2023,
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11(7):1798
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Flexicyte™ Assay
- Research area: Oncology
Background: Colorectal cancer (CRC) is one of the deadliest cancers worldwide and long-term survival is not guaranteed in metastatic disease despite current multidisciplinary therapies. A new compound 2,3,5,4′-Tetrahydroxystilbene (TG1), derived from THSG (2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-Glucoside), has been developed, and its anticancer ability...
View PublicationT Cells Expressing CAR Equipped With Extracellular Domain of BTLA Are Effective Against HVEM-over-expressing Melanoma Cell Lines
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Kim Y, Kim JE, Lee DY, et al. ,
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Anticancer Res ,
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2023,
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43(8):3419-3427
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background/aim: Several chimeric antigen receptor (CAR) T cells have been used to treat melanoma but have not shown favorable results. This study investigated whether Herpes virus entry mediator (HVEM), which is overexpressed in melanoma, is a potential novel antigen for...
View PublicationScalable manufacturing of gene-modified human mesenchymal stromal cells with microcarriers in spinner flasks
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Couto PS, Stibbs DJ, Rotondi MC, et al. ,
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Appl Microbiol Biotechnol ,
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2023,
-
- Instrument : NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Gene Therapy
Due to their immunomodulatory properties and in vitro differentiation ability, human mesenchymal stromal cells (hMSCs) have been investigated in more than 1000 clinical trials over the last decade. Multiple studies that have explored the development of gene-modified hMSC-based products are...
View PublicationNovel phenoxyacetylthiosemicarbazide derivatives as novel ligands in cancer diseases
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Kozyra P, Adamczuk G, Karczmarzyk Z, et al. ,
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Toxicol Appl Pharmacol ,
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2023,
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475:116634
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay, Mitochondrial Potential Assay
- Research area: Oncology
Numerous epidemiological studies report an increased risk of developing prostate cancer in patients with melanoma and an increased risk of developing melanoma in patients with prostate cancer. Based on our previous studies demonstrating the high anticancer activity of thiosemicarbazides with...
View PublicationThe TLR3 L412F polymorphism prevents TLR3-mediated tumor cell death induction in pediatric sarcomas
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Bisaccia J, Meyer S, Bertrand-Chapel A, et al. ,
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Cell Death Discov ,
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2023,
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9(1):230
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Toll-like receptor 3 (TLR3) is a pattern recognition receptor mainly known for its role in innate immune response to infection. Indeed, binding of double-stranded RNA (dsRNA) to TLR3 triggers a pro-inflammatory cascade leading to cytokine release and immune cell activation....
View PublicationPotent and uniform fetal hemoglobin induction via base editing
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Mayuranathan T, Newby GA, Feng R, et al. ,
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Nat Genet ,
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2023,
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55(7):1210-1220
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Inducing fetal hemoglobin (HbF) in red blood cells can alleviate β-thalassemia and sickle cell disease. We compared five strategies in CD34+ hematopoietic stem and progenitor cells, using either Cas9 nuclease or adenine base editors. The most potent modification was adenine...
View PublicationImmunomodulatory effects of seed peptide lunasin in RAW264.7 macrophages in obese microenvironments
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Huang P, Hsieh C ,
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Journal of Functional Foods ,
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2023,
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108:105719
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Immune Cells
- Assay: Cell Vitality Assay
- Research area: Cell Biology
In adiposity, immune cells infiltrate adipose tissues, especially macrophages, forming chronic inflammation. This study aimed to investigate the mechanism of lunasin regulating immune functions of RAW264.7 macrophages in obesity-related conditions. Lipopolysaccharide (LPS) triggered an acute inflammation, and adipocyte-conditioned medium (Ad-CM)...
View PublicationEmergent mechanical control of vascular morphogenesis
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Whisler J, Shahreza S, Schlegelmilch K, et al. ,
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Sci Adv ,
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2023,
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9(32):eadg9781
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Vascularization is driven by morphogen signals and mechanical cues that coordinately regulate cellular force generation, migration, and shape change to sculpt the developing vascular network. However, it remains unclear whether developing vasculature actively regulates its own mechanical properties to achieve...
View PublicationInhibition of the cholesterol transporter ABCA1 by probucol decreases capacitation and tyrosine phosphorylation of dog spermatozoa, and is dose dependent
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Schäfer-Somi S, Claaßen S, Lechner D ,
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Theriogenology ,
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2023,
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197:159-166
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
The ATP binding cassette (ABC) transporter molecule ABCA1 participates in the cholesterol transport within and through cell membranes. We recently demonstrated that in dog spermatozoa, capacitation could be decreased with probucol (PRO), an ABCA1 specific antagonist. In this study, a...
View PublicationThe Lipidomics of Spermatozoa and Red Blood Cells Membrane Profile of Martina Franca Donkey: Preliminary Evaluation
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Prasinou P, De Amicis I, Fusaro I, et al. ,
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Animals ,
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2023,
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13(1):8
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Fatty acid-based lipidomic analysis has been widely used to evaluate health status in human medicine as well as in the veterinary field. In equine species, there has been a developing interest in fertility and sperm quality. Fatty acids, being the...
View PublicationConcurrent Inhibition of Akt and ERK Using TIC-10 Can Overcome Venetoclax Resistance in Mantle Cell Lymphoma
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Granau AM, Andersen PA, Jakobsen T, et al. ,
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Cancers (Basel) ,
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2023,
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15(2):510
- Instrument : NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Venetoclax, a BCL-2 inhibitor, has proven to be effective in several hematological malignancies, including mantle cell lymphoma (MCL). However, development of venetoclax resistance is inevitable and understanding its underlying molecular mechanisms can optimize treatment response. We performed a thorough genetic,...
View PublicationPolypropylene nanoplastic exposure leads to lung inflammation through p38-mediated NF-κB pathway due to mitochondrial damage
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Woo JH, Seo HJ, Lee JY, et al. ,
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Part Fibre Toxicol ,
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2023,
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20(1):2
- Instrument : NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Polypropylene (PP) is used in various products such as disposable containers, spoons, and automobile parts. The disposable masks used for COVID-19 prevention mainly comprise PP, and the disposal of such masks is concerning because of the potential environmental pollution....
View PublicationEllagic Acid Prevents Particulate Matter-Induced Pulmonary Inflammation and Hyperactivity in Mice: A Pilot Study
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Jeong S, Bae S, Shin EC, et al. ,
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Int J Environ Res Public Health ,
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2023,
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20(5):4523
- Instrument : NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The inhalation of fine particulate matter (PM) is a significant health-related environmental issue. Previously, we demonstrated that repeated PM exposure causes hyperlocomotive activity in mice, as well as inflammatory and hypoxic responses in their lungs. In this study, we evaluated...
View PublicationEffects of egg yolk level, penetrating cryoprotectant, and pre-freeze cooling rate, on the post-thaw quality of stallion sperm
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Hernández-Avilés C, Ramírez-Agámez L, Varner DD, Love CC ,
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Anim Reprod Sci ,
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2023,
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248:107162
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
The current study determined the effect of the egg-yolk (phospholipid source) level (egg yolk [20% EY] vs. skim-milk + egg yolk [SM + 2% EY]), cryoprotectant (glycerol [Gly] vs. glycerol + methylformamide [Gly + MF]), and pre-freeze cooling rate (-0.1...
View PublicationEffects of stabilizer magnesium nirate on CMIT/MIT-induced respiratory toxicity
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Song MK, Baek YW, Kim DI, et al. ,
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Toxicol Res ,
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2023,
-
- Instrument : NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Despite a humidifier disinfectant (HD) product containing chloromethylisothiazolinone (CMIT) and methylisothiazolinone (MIT) with approximately 22% magnesium nitrate as a stabilizer, no report on the effects of magnesium nitrate on the respiratory toxicity of CMIT/MIT is available. In this study, Kathon...
View PublicationInhibition of lipid droplet accumulation by Solanum nigrum by suppressing adipogenesis and inducing lipolysis, thermogenesis and autophagy in 3T3‑L1 cells
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Choi JW, Choi HJ, Ryu GH, et al. ,
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Exp Ther Med ,
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2023,
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26(1):333
- Instrument : NucleoCounter® NC-250™
- Cell Type: Primary Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
It has been reported that Solanum nigrum exhibits anti-obesity effects in animal models induced by a high-fat diet. However, research on how Solanum nigrum exerts its anti-obesity effects is currently limited. Thus, the present study focused on identifying the mechanism...
View PublicationLipid alterations by oxidative stress increase detached acrosomes after cryopreservation of semen in Holstein bulls.
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Jakop U, Engel KM, Hürland M, et al. ,
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Theriogenology ,
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2023,
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197:37-45
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
The nearly exclusive use of cryopreserved semen in cattle breeding enables long shipping distances, higher storage times, quarantine to avoid germ transmission and easy dispersal of high genetic value bulls. Spermatozoa from bulls are well freezable and improvement of cryopreservation...
View PublicationChitin oligomers promote lymphoid innate and adaptive immune cell activation
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Maia A, Cardona Gloria Y, Fuchs K, et al. ,
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J Leukoc Biol ,
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2023,
-
qiad044
- Instrument : NucleoCounter® NC-250™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Chitin is a highly abundant N-acetyl-glucosamine (GlcNAc) polysaccharide which has been linked to immune responses in the context of fungal infections and allergic asthma, especially to T helper 2 (Th2) immune responses. Unfortunately, due to the frequent use of crude...
View PublicationThe use of machine learning methods to predict sperm quality in Holstein bulls
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Hürland M, Kuhlgatz DA, Kuhlgatz C, et al. ,
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Theriogenology ,
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2023,
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197:16-25
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
The aim of this study was to develop prediction models for total sperm motility, morphological abnormalities and sperm output based on 1,551 ejaculate records of 58 Holstein bulls. The data was collected from September 2019 to November 2020 in a...
View PublicationDuctal delivery of extracellular vesicles promote the recovery from salivary gland inflammation
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Kim D, Lim KM, Cho JM, et al. ,
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J Control Release ,
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2023,
-
357:235-248
- Instrument : NucleoCounter® NC-250™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, Cell Vitality Assay
- Research area: Stem Cell Research
Salivary gland dysfunction worsens the quality of life, but treatment for restoration of salivary gland function is limited. Although previous reports have demonstrated the therapeutic potentials of extracellular vesicles (EVs) in different preclinical models, the role of EVs in salivary...
View PublicationSperm factors associated with the production of equine blastocysts by intracytoplasmic sperm injection (ICSI) using frozen/thawed semen
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Ramírez-Agámez L, Hernández-Avilés C, Varner DD, Love CC ,
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Theriogenology ,
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2023,
-
195:85-92
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Intracytoplasmic Sperm Injection (ICSI) using frozen/thawed sperm is a common procedure to obtain embryos from fertile or subfertile mares and stallions. Stallion-associated factors that impact the efficiency of ICSI have been studied less than those associated with the mare. Three...
View PublicationThermostable Human Basic Fibroblast Growth Factor (TS-bFGF) Engineered with a Disulfide Bond Demonstrates Superior Culture Outcomes in Human Pluripotent Stem Cell
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Kim S, Kang GH, Lim KM, et al. ,
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Biology ,
-
2023,
-
12(6):888
- Instrument : NucleoCounter® NC-250™
- Cell Type: iPSCs & ESCs
- Assay: Cell Cycle Assay
- Research area: Stem Cell Research
Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) can differentiate into various tissues and are an essential source of various disease models and therapeutics. Various growth factors are required in order to culture pluripotent stem cells, among...
View PublicationFertility results after exocervical insemination using goat semen cryopreserved with extenders based on egg yolk, skim milk, or soybean lecithin
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Liang J, Larbi A, Lv C, et al. ,
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Reprod Domest Anim ,
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2023,
-
58(3):431-442
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
To evaluate the effects of four extenders on the post-thaw quality and fertility of goat semen, six Yunshang Black bucks’ semen was collected, pooled, diluted with Andromed® (Andr®), Optidyl® (Opt®), P3644 Sigma l-phosphatidylcholine (l-α SL), and skim milk-based (Milk) extenders,...
View PublicationEstablishment of an artificial particulate matter-induced lung disease model through analyzing pathological changes and transcriptomic profiles in mice
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Kim DI, Song MK, Yuk JE, et al. ,
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Sci Rep ,
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2023,
-
13(1):5955
- Instrument : NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Particulate matter (PM), an environmental risk factor, is linked with health risks such as respiratory diseases. This study aimed to establish an animal model of PM-induced lung injury with artificial PM (APM) and identify the potential of APM for toxicological...
View PublicationPaeonia lactiflora root decreases lipid accumulation through the induction of lipolysis and thermogenesis via AMPK activation in 3T3‑L1 cells
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Choi JW, Choi HJ, Ryu GH, et al. ,
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Int J Mol Med ,
-
2023,
-
52(2):65
- Instrument : NucleoCounter® NC-250™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Obesity is associated with high risk of mortality globally because obesity is associated with development of diseases such as diabetes, dyslipidemia, fatty liver disease, hypertension, and cancer. The present study aimed to identify the mechanism of action related to the...
View PublicationDual intron-targeted CRISPR-Cas9-mediated disruption of the AML RUNX1-RUNX1T1 fusion gene effectively inhibits proliferation and decreases tumor volume in vitro and in vivo
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Neldeborg S, Soerensen JF, Møller CT, et al. ,
-
Leukemia ,
-
2023,
-
- Instrument : NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Oncogenic fusion drivers are common in hematological cancers and are thus relevant targets of future CRISPR-Cas9-based treatment strategies. However, breakpoint-location variation in patients pose a challenge to traditional breakpoint-targeting CRISPR-Cas9-mediated disruption strategies. Here we present a new dual intron-targeting CRISPR-Cas9...
View PublicationMitochondrial dysfunction and mitophagy defects in LRRK2-R1441C Parkinson’s disease models
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Williamson MG, Madureira M, McGuinness W, et al. ,
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Hum Mol Genet ,
-
2023,
-
ddad102
- Instrument : NucleoCounter® NC-250™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Mutations in the Leucine-Rich Repeat Kinase 2 (LRRK2) gene have been identified as one of the most common genetic causes of Parkinson’s disease (PD). The LRRK2 PD-associated mutations LRRK2G2019S and LRRK2R1441C, located in the kinase domain and in the ROC-COR...
View PublicationSperm parameters in the Great Dane: Influence of age on semen quality
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Foutouhi A, Hesser A, de la Fuente A, et al. ,
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Theriogenology ,
-
2023,
-
197:267-274
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Not all sires have sperm suitable for chilled or frozen storage, and success in artificial insemination (AI) varies highly among individual dogs and breeds. Fertilizing potential is further complicated as sperm quality declines with the aging process. Due to the...
View PublicationDiagnosis and management of spermiostasis in a Shetland stallion
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Frink AR, Podico G, Canisso IF ,
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Equine Veterinary Education ,
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2023,
-
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Spermiostasis (or sperm accumulation) is an ejaculatory dysfunction of common occurrence in referral reproductive practice with stallions. The accumulation of sperm in the epididymides, ducti deferens, and ampullae results in ejaculates with a high concentration of degenerated sperm. It is...
View PublicationReconstitution and post-thaw storage of cryopreserved human mesenchymal stromal cells: Pitfalls and optimizations for clinically compatible formulants
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Aabling RR, Alstrup T, Kjær EM, et al. ,
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Regen Ther ,
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2023,
-
23:67-75
- Instrument : NucleoCounter® NC-202™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Introduction: The regenerative and immunomodulatory properties of multipotent mesenchymal stromal cells (MSCs) make them an intriguing asset for therapeutic applications. An off-the-shelf approach, using pre-expanded cryopreserved allogenic MSCs, bypasses many practical difficulties of cellular therapy. Reconstitution of a MSC product...
View PublicationSemen Quality of the First and Second Ejaculates Collected from Breeding Inactive Stallions after Cooling and Freezing
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Podico G, Spencer KM, Magalhaes HB, Canisso IF ,
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Vet Sci ,
-
2023,
-
10(3):173
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
This study aimed to assess the semen quality after the cooling and freezing of the first and second ejaculates of the season, which were collected 1 h apart. After collection (n = 40 ejaculates), the gel-free semen volume, concentration, total...
View PublicationEnhanced bioprocess control to advance the manufacture of mesenchymal stromal cell-derived extracellular vesicles in stirred-tank bioreactors
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Costa MHG, Costa MS, Painho B, et al. ,
-
Biotechnol Bioeng ,
-
2023,
-
- Instrument : NucleoCounter® NC-202™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) act as signaling mediators of cellular responses. However, despite representing a promising alternative to cell-based therapies, clinical translation of EVs is currently limited by their lack of scalability and standardized bioprocessing....
View PublicationDeciphering sperm chromatin properties to predict stallion sperm fertility
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Al-Kass Z, Ntallaris T, Morrell JM, Johannisson A ,
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Anim Reprod Sci ,
-
2023,
-
250:107200
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Although previous studies have examined the relationship between the sperm DNA fragmentation index and fertility in stallions, other aspects of chromatin structure or packaging and fertility have not been explored. In the present study, relationships between fertility and DNA fragmentation...
View PublicationFibroblast activation protein targeted radiotherapy induces an immunogenic tumor microenvironment and enhances the efficacy of PD-1 immune checkpoint inhibition
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Zboralski D, Osterkamp F, Christensen E, et al. ,
-
Eur J Nucl Med Mol Imaging ,
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2023,
-
- Instrument : NucleoCounter® NC-202™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Purpose: FAP is a membrane-bound protease under investigation as a pan-cancer target, given its high levels in tumors but limited expression in normal tissues. FAP-2286 is a radiopharmaceutical in clinical development for solid tumors that consists of two functional elements:...
View PublicationEffect of Some Plant-Based Substances on Microbial Content and Sperm Quality Parameters of Bull Semen
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Cojkic A, Hansson I, Johannisson A, Morrell JM ,
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Int J Mol Sci ,
-
2023,
-
24(4):3435
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
The rapid emergence of antibacterial resistance requires alternatives to antibiotics to be found, including for semen preservation. One of the possible alternatives would be to use plant-based substances with known antimicrobial effects. The objective of this study was to test...
View PublicationHigh-Throughput Production of Microcapsules for Human Bone Marrow Derived Mesenchymal Stem Cell Biomanufacturing in a Vertical-Wheel Bioreactor
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Teryek M, Jadhav P, Bento R, Parekkadan B ,
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Biotechnology and Bioprocess Engineering ,
-
2023,
-
- Instrument : NucleoCounter® NC-202™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Microencapsulation of human mesenchymal stromal cells (MSCs) via electrospraying has been well documented in tissue engineering and regenerative medicine. Herein, we report the use of microencapsulation, via electrospraying, for MSC expansion using a commercially available hydrogel that is durable, optimized...
View PublicationAnimal board invited review: An update on the methods for semen quality evaluation in swine – from farm to the lab
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Maside C, Recuero S, Salas-Huetos A, et al. ,
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Animal ,
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2023,
-
17(3):100720
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Pig breeding is mainly conducted through artificial insemination with liquid-stored semen. It is, therefore, crucial to ensure that sperm quality is over the standard thresholds, as reduced sperm motility, morphology or plasma membrane integrity are associated with reduced farrowing rates...
View PublicationSpecialized functions and sexual dimorphism explain the functional diversity of the myeloid populations during glioma progression
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Ochocka N, Segit P, Wojnicki K, et al. ,
-
Cell Rep ,
-
2023,
-
42(1):111971
- Instrument : NucleoCounter® NC-200™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Malignant gliomas are aggressive, hard-to-treat brain tumors. Their tumor microenvironment is massively infiltrated by myeloid cells, mostly brain-resident microglia, bone marrow (BM)-derived monocytes/macrophages, and dendritic cells that support tumor progression. Single-cell omics studies significantly dissected immune cell heterogeneity, but dynamics...
View PublicationAn Experimental Anodized Titanium Surface for Transgingival Dental Implant Elements-Preliminary Report
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Hadzik J, Kubasiewicz-Ross P, Gębarowski T, et al. ,
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J Funct Biomater ,
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2023,
-
14(1):34
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The characteristics such as microtopography, physical and chemical properties influence the behavior of an implant in a soft tissue. Anodization-as a potent method of titanium alloy surface modification-of the transgingival abutment or healing screw, has achieved some improvement. One of...
View PublicationNon-destructive classification of unlabeled cells: Combining an automated benchtop magnetic resonance scanner and artificial intelligence
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Fey P, Weber DL, Stebani J, et al. ,
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PLoS Comput Biol ,
-
2023,
-
19(2):e1010842
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
In order to treat degenerative diseases, the importance of advanced therapy medicinal products has increased in recent years. The newly developed treatment strategies require a rethinking of the appropriate analytical methods. Current standards are missing the complete and sterile analysis...
View PublicationInvestigation into the use of gamma irradiated Cytodex-1 microcarriers to produce a human cytomegalovirus (HCMV) vaccine candidate in epithelial cells
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Investigation into the use of gamma irradiated Cytodex- microcarriers to produce a human cytomegalovirus (HCMV) vaccine candidate in epithelial cells ,
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J Biotechnol ,
-
2023,
-
365:62-71
- Instrument : NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Virus Production
V160 is a viral vaccine candidate against human cytomegalovirus (HCMV) that is manufactured using Adult Retinal Pigment Epithelial cells (ARPE-19) grown on Cytodex-1 microcarriers. The microcarriers are generally hydrated, washed, and autoclaved prior to use, which can be limiting at...
View PublicationHarmonised culture procedures minimise but do not eliminate mesenchymal stromal cell donor and tissue variability in a decentralised multicentre manufacturing approach
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Calcat-I-Cervera S, Rendra E, Scaccia E, et al. ,
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Stem Cell Res Ther ,
-
2023,
-
14(1):120
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Background: Mesenchymal stromal cells (MSCs), commonly sourced from adipose tissue, bone marrow and umbilical cord, have been widely used in many medical conditions due to their therapeutic potential. Yet, the still limited understanding of the underlying mechanisms of action hampers...
View PublicationCharacterization of human induced pluripotent stems cells: Current approaches, challenges, and future solutions
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Suresh Babu S, Duvvuru H, Baker J, et al. ,
-
Biotechnol Rep (Amst) ,
-
2023,
-
37:e00784
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Human induced pluripotent stem cells (iPSC) have demonstrated massive potentials for use in regenerative and personalized medicine due to their ability to expand in culture and differentiate into specialized cells with therapeutic benefits. However, in order to industrialize iPSC-derived therapies,...
View PublicationSpecific in situ immuno-imaging of pulmonary-resident memory lymphocytes in human lungs
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Humphries DC, O'Connor RA, Stewart HL, et al. ,
-
Front Immunol ,
-
2023,
-
14:1100161
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based immunotherapy
Introduction: Pulmonary-resident memory T cells (TRM) and B cells (BRM) orchestrate protective immunity to reinfection with respiratory pathogens. Developing methods for the in situ detection of these populations would benefit both research and clinical settings. Methods: To address this need,...
View PublicationExpansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation
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Wong YF, Kumar Y, Proks M, et al. ,
-
Nat Cell Biol ,
-
2023,
-
25(3):481-492
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Cell proliferation is fundamental for almost all stages of development and differentiation that require an increase in cell number. Although cell cycle phase has been associated with differentiation, the actual process of proliferation has not been considered as having a...
View PublicationHarmonization and standardization of nucleus pulposus cell extraction and culture methods
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Basatvat S, Bach FC, Barcellona MN, et al. ,
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JOR Spine ,
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2023,
-
6(1):e1238
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: In vitro studies using nucleus pulposus (NP) cells are commonly used to investigate disc cell biology and pathogenesis, or to aid in the development of new therapies. However, lab-to-lab variability jeopardizes the much-needed progress in the field. Here, an...
View PublicationImprovement of Antioxidant Defences in Keratinocytes Grown in Physioxia: Comparison of 2D and 3D Models
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Chettouh-Hammas N, Fasani F, Boileau A, et al. ,
-
Oxidative Medicine and Cellular Longevity ,
-
2023,
-
6829931
- Instrument : NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Keratinocytes prevent skin photoaging by ensuring the defence against oxidative stress, an excessive production of reactive oxygen species (ROS). They are localized within the epidermis where the oxygen level (1-3% O2), named physioxia, is low compared to other organs. Oxygen...
View PublicationResponse to oxidative stress of AML12 hepatocyte cells with knockout of methionine sulfoxide reductases
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Trujillo-Hernandez JA, Levine RL ,
-
Free Radic Biol Med ,
-
2023,
-
205:100-106
- Instrument : NucleoCounter® NC-200™
- Cell Type: Hepatocytes
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Methionine sulfoxide reductases are enzymes that reduce methionine oxidation in the cell. In mammals there are three B-type reductases that act on the R-diastereomer of methionine sulfoxide, and one A-type reductase (MSRA) that acts on the S-diastereomer. Unexpectedly, knocking out...
View PublicationRelationship between oxygen partial pressure and inhibition of cell aggregation of human adipose tissue-derived mesenchymal stem cells stored in cell preservation solutions
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Kikuchi T, Nishimura M, Shirakawa C, et al. ,
-
Regen Ther ,
-
2023,
-
24:25-31
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Introduction: This study investigated the storage conditions under which cell aggregation occurs and the conditions that inhibit cell aggregation when human adipose tissue-derived mesenchymal stem cells (hADSCs) are stored in lactated Ringer’s solution (LR) supplemented with 3% trehalose and 5%...
View PublicationA cell culture system to model pharmacokinetics using adjustable-volume perfused mixing chambers
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Erickson P, Jetley G, Amin P, et al. ,
-
Toxicol In Vitro ,
-
2023,
-
91:105623
- Instrument : NucleoCounter® NC-200™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
The pharmacokinetic (PK) profile of a drug is an essential factor in determining its efficacy, yet it is often neglected during in vitro cell culture experiments. Here, we present a system in which standard well plate cultures may be “plugged...
View PublicationDevelopment of a perfusion process for continuous lentivirus production using stable suspension producer cell lines
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Klimpel M, Terrao M, Ching N, et al. ,
-
Biotechnol Bioeng ,
-
2023,
-
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Virus Production
The large-scale production of clinical-grade lentiviral vectors (LVs) for gene therapy applications is a remaining challenge. The use of adherent cell lines and methods like transient transfection are cost-intensive and hamper process scalability as well as reproducibility. This study describes...
View PublicationInfection with Seasonal H1N1 Influenza Results in Comparable Disease Kinetics and Host Immune Responses in Ferrets and Golden Syrian Hamsters
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Paterson J, Ryan KA, Morley D, et al. ,
-
Pathogens ,
-
2023,
-
12(5):668
- Instrument : NucleoCounter® NC-200™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Animal models of influenza are important in preclinical research for the study of influenza infection and the assessment of vaccines, drugs and therapeutics. Here, we show that Golden Syrian hamsters (Mesocricetus auratus) inoculated via the intranasal route with high dose...
View PublicationImprovement of HEK293 Cell Growth by Adapting Hydrodynamic Stress and Predicting Cell Aggregate Size Distribution
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Seidel S, Maschke RW, Mozaffari F, et al. ,
-
Bioengineering ,
-
2023,
-
10(4):478
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
HEK293 is a widely used cell line in the fields of research and industry. It is assumed that these cells are sensitive to hydrodynamic stress. The aim of this research was to use particle image velocimetry validated computational fluid dynamics...
View PublicationDifferences in allergen‐specific basophil activation and T cell proliferation in atopic dermatitis patients with comorbid allergic rhinoconjunctivitis treated with a monoclonal anti‐IL‐4Rα antibody or allergen‐specific immunotherapy
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Layritz AS, Galicia-Carreón J, Benfadal S, Novak N ,
-
Immun Inflamm Dis ,
-
2023,
-
11(4):e808
- Instrument : NucleoCounter® NC-200™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell-based immunotherapy
Background: Atopic dermatitis (AD), a chronic inflammatory disorder, is often accompanied by allergic rhinoconjunctivitis (ARC) as a co-morbidity. The use of a monoclonal anti-IL-4Rα antibody has been effective in controlling moderate to severe AD symptoms. Allergen-specific immunotherapy (AIT) is widely...
View PublicationPD-L1 blockade in combination with carboplatin as immune induction in metastatic lobular breast cancer: the GELATO trial
-
Voorwerk L, Isaeva OI, Horlings HM, et al. ,
-
Nat Cancer ,
-
2023,
-
4(4):535-549
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Invasive lobular breast cancer (ILC) is the second most common histological breast cancer subtype, but ILC-specific trials are lacking. Translational research revealed an immune-related ILC subset, and in mouse ILC models, synergy between immune checkpoint blockade and platinum was observed....
View PublicationInhibition of a Microbiota-Derived Peptide Ameliorates Established Acute Lung Injury
-
Fridman D'Alessandro V, D'Alessandro-Gabazza CN, Yasuma T, et al. ,
-
Am J Pathol ,
-
2023,
-
193(6):740-754
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
Acute lung injury (ALI) is a clinical syndrome characterized by a diffuse lung inflammation that commonly evolves into acute respiratory distress syndrome and respiratory failure. The lung microbiota is involved in the pathogenesis of ALI. Corisin, a proapoptotic peptide derived...
View PublicationMagnesium implant degradation provides immunomodulatory and proangiogenic effects and attenuates peri-implant fibrosis in soft tissues
-
Ben Amara H, Martinez DC, Shah FA, et al. ,
-
Bioact Mater ,
-
2023,
-
26:353-369
- Instrument : NucleoCounter® NC-200™
- Cell Type: Primary Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Implants made of magnesium (Mg) are increasingly employed in patients to achieve osteosynthesis while degrading in situ. Since Mg implants and Mg2+ have been suggested to possess anti-inflammatory properties, the clinically observed soft tissue inflammation around Mg implants is enigmatic....
View PublicationAcceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cells
-
Zhou Z, Xun J, Wu C, et al. ,
-
Mater Today Bio ,
-
2023,
-
20:100686
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Umbilical cord-derived mesenchymal stem cells (UC-MSC) are promising candidates for wound healing. However, the low amplification efficiency of MSC in vitro and their low survival rates after transplantation have limited their medical application. In this study, we fabricated a micronized...
View PublicationEstablishing an experimental Pseudomonas aeruginosa keratitis model in mice – Challenges and solutions
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Englisch CN, Wadood NA, Pätzold L, et al. ,
-
Ann Anat ,
-
2023,
-
249:152099
- Instrument : NucleoCounter® NC-200™
- Cell Type: Primary Cells
- Assay: Cell Count & Viability Assay
- Research area: Ophthalmology
Background: With the ongoing increase in antimicrobial resistances seen in bacterial isolates causing a keratitis in humans, animal models have become an important tool to study new antimicrobial therapies. Nevertheless, the establishment of experimental keratitis is difficult. Here, we discuss...
View PublicationAggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applications
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Yuen JSK Jr, Saad MK, Xiang N, et al. ,
-
Elife ,
-
2023,
-
12:e82120
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cultured Meat
We present a method of producing bulk cell-cultured fat tissue for food applications. Mass transport limitations (nutrients, oxygen, waste diffusion) of macroscale 3D tissue culture are circumvented by initially culturing murine or porcine adipocytes in 2D, after which bulk fat...
View PublicationA protective, single-visit TB vaccination regimen by co-administration of a subunit vaccine with BCG
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Dijkman K, Lindenstrøm T, Rosenkrands I, et al. ,
-
NPJ Vaccines ,
-
2023,
-
8(1):66
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
The only licensed tuberculosis (TB) vaccine, Bacillus Calmette Guerin (BCG), fails to reliably protect adolescents and adults from pulmonary TB, resulting in ~1.6 million deaths annually. Protein subunit vaccines have shown promise against TB in clinical studies. Unfortunately, most subunit...
View PublicationPioneer factor ASCL1 cooperates with the mSWI/SNF complex at distal regulatory elements to regulate human neural differentiation
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Păun O, Tan YX, Patel H, et al. ,
-
Genes Dev ,
-
2023,
-
37(5-6):218-242
- Instrument : NucleoCounter® NC-200™
- Cell Type: Neural Cells
- Assay: Cell Count & Viability Assay
- Research area: Neurobiology
Pioneer transcription factors are thought to play pivotal roles in developmental processes by binding nucleosomal DNA to activate gene expression, though mechanisms through which pioneer transcription factors remodel chromatin remain unclear. Here, using single-cell transcriptomics, we show that endogenous expression...
View PublicationYoung glial progenitor cells competitively replace aged and diseased human glia in the adult chimeric mouse brain
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Vieira R, Mariani JN, Huynh NPT, et al. ,
-
Nat Biotechnol ,
-
2023,
-
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Neurobiology
Competition among adult brain cells has not been extensively researched. To investigate whether healthy glia can outcompete diseased human glia in the adult forebrain, we engrafted wild-type (WT) human glial progenitor cells (hGPCs) produced from human embryonic stem cells into...
View PublicationRecommendations for intervertebral disc notochordal cell investigation: From isolation to characterization
-
Williams RJ, Laagland LT, Bach FC, et al. ,
-
JOR Spine ,
-
2023,
-
- Instrument : NucleoCounter® NC-200™
- Cell Type: Primary Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background Lineage-tracing experiments have established that the central region of the mature intervertebral disc, the nucleus pulposus (NP), develops from the embryonic structure called “the notochord”. However, changes in the cells derived from the notochord which form the NP (i.e.,...
View PublicationMG53 alleviates airway inflammatory responses by regulating nuclear factor-κB pathway in asthmatic mice
-
Tan S, Li M, Song X ,
-
Allergol Immunopathol (Madr) ,
-
2023,
-
51(4):175-181
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Asthma is a common lung disease with increasing incidence and prevalence globally, thereby imposing a substantial global health and economic burden. Recently, studies have shown that Mitsugumin 53 (MG53) exhibits multiple biological functions and plays a protective role in...
View PublicationAlveolar epithelial-like cell differentiation in a dynamic bioreactor: a promising 3D-approach for the high-throughput generation of lung cell types from human induced pluripotent stem cells
-
Müller M, Kohl Y, Germann A, et al. ,
-
In vitro models ,
-
2023,
-
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Purpose Human induced pluripotent stem cell (hiPSC)-derived lung cell types such as alveolar epithelial cells are promising for toxicological and pharmaceutical in vitro screenings. Reproducible differentiation processes are highly demanded, but protocols which are suitable for the high-throughput generation of...
View PublicationNumbers Matter: The Role of Cell Dose in the Treatment of Osteosarcoma Using Mesenchymal Stromal Cells as Cellular Vehicles
-
Martella E, Dozza B, Ferroni C, et al. ,
-
Adv Therap ,
-
2023,
-
2300045
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Oncology
A promising approach enhancing osteosarcoma (OS) prognosis involves the combination of various techniques, such as chemo- and photodynamic therapy, delivered through nanocarriers for synergistic cell death. Among the potential candidates for improving drug accumulation at the tumor site, mesenchymal stromal...
View PublicationCardioMotion: identification of functional and structural cardiotoxic liabilities in small molecules through brightfield kinetic imaging
-
Stebbeds W, Raniga K, Standing D, et al. ,
-
Toxicol Sci ,
-
2023,
-
kfad065
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Cardiology
Cardiovascular toxicity is an important cause of drug failures in the later stages of drug development, early clinical safety assessment and even post-market withdrawals. Early-stage in vitro assessment of potential cardiovascular liabilities in the pharmaceutical industry involves assessment of interactions...
View PublicationGeneration of the NeoThy mouse model for human immune system studies
-
Del Rio NM, Huang L, Murphy L, et al. ,
-
Lab Anim ,
-
2023,
-
149-168
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Humanized mouse models, created via transplantation of human hematopoietic tissues into immune-deficient mice, support a number of research applications, including transplantation immunology, virology and oncology studies. As an alternative to the bone marrow, liver, thymus humanized mouse, which uses fetal...
View PublicationMammalian Fuel Cells Produce Electric Current
-
Shlosberg Y, Faynus MA, Huang A, et al. ,
-
ACS Appl Mater Interfaces ,
-
2023,
-
15(29):34992-35000
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Ophthalmology
The increasing concern about climate change has led scientists around the world to develop clean energy technologies that may replace the traditional use of fossil fuels. A promising approach is the utilization of unicellular organisms as electron donors in bio-fuel...
View PublicationSemi-synthetic degradable notochordal cell-derived matrix hydrogel for use in degenerated intervertebral discs: Initial in vitro characterization
-
Schmitz TC, van Genabeek B, Pouderoijen MJ, et al. ,
-
J Biomed Mater Res A ,
-
2023,
-
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Low back pain is the leading cause of disability worldwide, but current therapeutic interventions are palliative or surgical in nature. Loss of notochordal cells (NCs) and degradation of the healthy matrix in the nucleus pulposus (NP), the central tissue of...
View PublicationA matter of agreement: The effect of the technique and evaluator on the analysis of morphologic defects in stallion sperm
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Hernández-Avilés C, Ramírez-Agámez L, Pearson M, et al. ,
-
Theriogenology ,
-
2023,
-
202:74-83
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Analysis of sperm morphology is an important part of the stallion breeding soundness evaluation since it provides an objective measure of a stallion’s sperm quality and is one of many factors that estimate a stallion’s fertility potential. To describe the...
View PublicationInfluence of age, breed, and season on the quality of boar semen stored at low-temperature
-
Hensel B, Pieper L, Jung M, Schulze M ,
-
Theriogenology ,
-
2023,
-
208:102-108
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
In the face of antimicrobial resistance, antibiotic-free, low-temperature storage of boar semen has been well-researched in recent years and promising results have been obtained. With the prospect of establishing this new preservation method in practice, it is important to evaluate...
View PublicationOvine fertility by artificial insemination in the breeding season could be affected by intraseasonal variations in ram sperm proteomic profile
-
Neila-Montero M, Alvarez M, Riesco MF, et al. ,
-
Theriogenology ,
-
2023,
-
208:28-42
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
It is important to note that seasonality could affect ram reproductive parameters, and therefore, fertility results after artificial insemination. In this work, 1) we assessed fertility rates after cervical artificial insemination of 11,805 ewes at the beginning (June 21st to...
View PublicationMicrobiome structure of milt and ovarian fluid in farmed Arctic charr (Salvelinus alpinus)
-
Palaiokostas C, Gohar D, Kurta K, et al. ,
-
Aquaculture ,
-
2023,
-
739648
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Limited knowledge exists about the residing microbiome in gamete-related samples in fish. A potential effect between the seminal microbiome composition and sperm quality traits has been previously suggested in humans and livestock. Using a metabarcoding approach, we aimed to gain...
View PublicationSuccessful use of sex-sorted frozen semen in a commercial OPU-ICSI program
-
Ducheyne KD, Humphreys p, Colleoni S, et al. ,
-
Journal of Equine Veterinary Science ,
-
2023,
-
150:104647
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
There is increasing interest in the use of sex-sorted semen especially in certain breeds. After artificial insemination (AI) with fresh sex-sorted semen, pregnancy rates between 10% and 60% have been reported (Buchanan. et. al. Theriogenology. 2000; 53:1309-1321; Lindsey. et. al....
View PublicationLactate as the sole energy substrate induces spontaneous acrosome reaction in viable stallion spermatozoa
-
Ramírez-Agámez L, Hernández-Avilés C, Ortíz I, et al. ,
-
Andrology ,
-
2023,
-
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Background: Equine spermatozoa appear to differ from spermatozoa of other species in using oxidative phosphorylation preferentially over glycolysis. However, there is little information regarding effects of different energy sources on measured parameters in equine spermatozoa. Objective: To determine the effect...
View PublicationThe effect of sex-sorting on stallion semen quality
-
Samper JC, Love CC, Hernandez-Aviles C, et al. ,
-
Journal of Equine Veterinary Science ,
-
2023,
-
125:104678
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Spermatozoa retain fertilizing potential after sorting, but separation into X and Y populations has deleterious effects on the cell (Samper JC et al. Clinical Theriogenology 2022). The effect of sex-sorting (SS) on stallion sperm was evaluated in single ejaculates from...
View PublicationShipping duration and temperature influence the characteristics of cryopreserved horse semen stored in different shipping devices for up to 14 days
-
El-Shalofy A, Gautier C, Khan Y, et al. ,
-
Anim Reprod Sci ,
-
2023,
-
256:107307
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
This study aimed to investigate the effects of storing horse semen either in a dry shipper (≤ -150 °C) or on dry ice (≤ -78 °C) for up to 14 days. A total of 264 frozen semen straws from male...
View PublicationRho-associated coiled-coil containing kinase inhibitor improves outcomes of direct-transfer slow-cooled bovine blastocysts
-
Abdelhady AWA, Aguiar LH, Lee YL, et al. ,
-
Theriogenology ,
-
2023,
-
211:19-27
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Direct-transfer slow-cooling cryopreservation is a widely used method for bovine embryo cryopreservation. However, the transfer of cryopreserved embryos is associated with reduced pregnancy rates. Rho-associated coiled-coil containing kinase inhibitor (ROCKi) has shown promise in improving the viability of post-warmed vitrified...
View PublicationUse of Unmalted and Malted Buckwheat in Brewing
-
Ciocan ME, Salamon RV, Ambrus Á, et al. ,
-
Applied Sciences ,
-
2023,
-
13(4):2199
- Instrument : NucleoCounter® YC-100™
- Cell Type: Yeast Cells
- Assay: Cell Count & Viability Assay
- Research area: Brewing
With the goal of introducing specialty beers with full flavor and effective processing, craft and functional beer have revived old assortments and established new assortments in countries throughout the world. These unique selections are produced utilizing various ingredient combinations or...
View PublicationComparison of Omentum and Subcutis as Implant Sites for Device-Encapsulated Human iPSC-Derived Pancreatic Endoderm in Nude Rats
-
Nijns JR, De Mesmaeker I, Suenens KG, et al. ,
-
Cell Transplant ,
-
2023,
-
32:9636897231167323
- Instrument : NucleoCounter® YC-100™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Subcutaneous implants of device-encapsulated stem cell-derived pancreatic endoderm (PE) can establish a functional beta cell mass (FBM) with metabolic control in immune-compromised mice. In a study with human-induced pluripotent stem cell-PE, this outcome was favored by a preformed pouch which...
View PublicationBrewing with Unmalted and Malted Sorghum: Influence on Beer Quality
-
Ciocan ME, Salamon RV, Ambrus Á, et al. ,
-
Fermentation ,
-
2023,
-
9(5):490
- Instrument : NucleoCounter® YC-100™
- Cell Type: Yeast Cells
- Assay: Cell Count & Viability Assay
- Research area: Brewing
One of the earliest biotechnological processes is brewing, which uses conventional raw materials like barley malt and, to a lesser extent, wheat malt. Today, adjuncts are used in the brewing of 85–90% of the world’s beer, with significant regional differences....
View PublicationImmunological and Oxidative Biomarkers in Bovine Serum from Healthy, Clinical, and Sub-Clinical Mastitis Caused by Escherichia coli and Staphylococcus aureus Infection
-
Sadat A, Farag AMM, Elhanafi D, et al. ,
-
Animals ,
-
2023,
-
13(5):892
- Instrument : NucleoCounter® SCC-100™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Dairy (SCC)
The study aimed to investigate the mastitis’ emerging causative agents and their antimicrobial sensitivity, in addition to the hematological, biochemical indicators, oxidative biomarkers, acute phase protein (APP), and inflammatory cytokine changes in dairy farms in Gamasa, Dakahlia Governorate, Egypt. One...
View PublicationThe Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate Using a Point of Care System Does Not Depend on the Subject’s Individual Age, Sex, Body Mass Index and Ethnicity
-
Schmitz C, Alt C, Azares AR, et al. ,
-
Cells ,
-
2023,
-
12(1):30
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Uncultured, unmodified, autologous, adipose-derived regenerative cells (UA-ADRCs) are a safe and effective treatment option for various musculoskeletal pathologies. However, it is unknown whether the composition of the final cell suspension systematically varies with the subject’s individual age, sex, body mass...
View PublicationCellular and humoral immune responsiveness to inactivated Leptospira interrogans in dogs vaccinated with a tetravalent Leptospira vaccine
-
Novak A, Hindriks E, Hoek A, et al. ,
-
Vaccine ,
-
2023,
-
41(1):119-129
- Instrument : NucleoCounter® NC-200™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell-based immunotherapy
Vaccination is commonly used to protect dogs against leptospirosis, however, memory immune responses induced by canine Leptospira vaccines have not been studied. In the present study, antibody and T cell mediated responses were assessed in dogs before and 2 weeks...
View PublicationChemical derivatization as a novel strategy for selective and sensitive determination of intracellular di-and triphosphate anabolites in peripheral blood mononuclear cells
-
Fang K, Licea-Perez H. Chemical derivatization as a novel strategy for selective and sensitive determination of intracellular di-and triphosphate anabolites in peripheral blood mononuclear cells ,
-
J Pharm Biomed Anal ,
-
2023,
-
223:115124
- Instrument : NucleoCounter® NC-200™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Quantitation of intracellular tri-phosphate anabolite, GSK1-TP, was required to understand drug efficacy of a proprietary molecule, GSK1; while quantitation of the di-phosphate, GSK1-DP, provided an indicator of potential GSK1-TP instability during sample processing and storage. A novel derivatization approach with...
View PublicationIL-5-producing CD4+ T cells and eosinophils cooperate to enhance response to immune checkpoint blockade in breast cancer
-
Blomberg OS, Spagnuolo L, Garner H, et al. ,
-
Cancer Cell ,
-
2023,
-
41(1):106-123.e10
- Instrument : NucleoCounter® NC-200™
- Cell Type: Whole Blood
- Assay: Cell Count & Viability Assay
- Research area: Cell-based immunotherapy
Immune checkpoint blockade (ICB) has heralded a new era in cancer therapy. Research into the mechanisms underlying response to ICB has predominantly focused on T cells; however, effective immune responses require tightly regulated crosstalk between innate and adaptive immune cells....
View PublicationSafety and efficacy of first-in-man intrathecal injection of human astrocytes (AstroRx®) in ALS patients: phase I/IIa clinical trial results
-
Gotkine M, Caraco Y, Lerner Y, et al. ,
-
J Transl Med ,
-
2023,
-
21(1):122
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Neurobiology
Background: Malfunction of astrocytes is implicated as one of the pathological factors of ALS. Thus, intrathecal injection of healthy astrocytes in ALS can potentially compensate for the diseased astrocytes. AstroRx® is an allogeneic cell-based product, composed of healthy and functional...
View PublicationEndogenous IL-1 receptor antagonist restricts healthy and malignant myeloproliferation
-
Villatoro A, Cuminetti V, Bernal A, et al. ,
-
Nat Commun ,
-
2023,
-
14(1):12
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based immunotherapy
Here we explored the role of interleukin-1β (IL-1β) repressor cytokine, IL-1 receptor antagonist (IL-1rn), in both healthy and abnormal hematopoiesis. Low IL-1RN is frequent in acute myeloid leukemia (AML) patients and represents a prognostic marker of reduced survival. Treatments with...
View PublicationRTA-dh404 decreased oxidative stress in mice ischemic limbs and augmented efficacy of therapeutic angiogenesis by intramuscular injection of adipose-derived regenerative cells in the limbs
-
Ishizaki Y, Sasaki KI, Yoshikawa T, et al. ,
-
Eur J Pharmacol ,
-
2023,
-
938:175422
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Although an intramuscular injection of angiogenic cells to ischemic limbs with peripheral artery disease is a therapeutic option to rescue patients by augmenting neovascularization in the limbs, oxidative stress in the limbs may accelerate apoptosis of the injected cells and...
View PublicationPloidy-stratified single cardiomyocyte transcriptomics map Zinc Finger E-Box Binding Homeobox 1 to underly cardiomyocyte proliferation before birth
-
Bak ST, Harvald EB, Ellman DG, et al. ,
-
Basic Res Cardiol ,
-
2023,
-
118(1):8
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cardiology
Whereas cardiomyocytes (CMs) in the fetal heart divide, postnatal CMs fail to undergo karyokinesis and/or cytokinesis and therefore become polyploid or binucleated, a key process in terminal CM differentiation. This switch from a diploid proliferative CM to a terminally differentiated...
View PublicationCerium oxide nanoparticles disseminated chitosan gelatin scaffold for bone tissue engineering applications
-
Bhushan S, Singh S, Maiti TK, et al. ,
-
Int J Biol Macromol ,
-
2023,
-
236:123813
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Cell-free and cell-loaded constructs are used to bridge the critical-sized bone defect. Oxidative stress at the site of the bone defects is a major interference that slows bone healing. Recently, there has been an increase in interest in enhancing the...
View PublicationSurgical cotton microfibers loaded with proteins and apatite: A potential platform for bone tissue engineering
-
Singh S, Bhushan S, Khan H, et al. ,
-
Int J Biol Macromol ,
-
2023,
-
236:123812
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Tissue engineering has emerged as the best alternative to replacing damaged tissue/organs. However, the cost of scaffold materials continues to be a significant obstacle; thus, developing inexpensive scaffolds is strongly encouraged. In this study, cellulose microfibers (C), gelatin (G), egg...
View PublicationC57Bl/6N mice have an attenuated lung inflammatory response to dsRNA compared to C57Bl/6J and BALB/c mice
-
Malm Tillgren S, Nieto-Fontarigo JJ, Cerps S, et al. ,
-
J Inflamm ,
-
2023,
-
20(1):6
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based immunotherapy
Background: Lower respiratory infections caused by ssRNA viruses are a major health burden globally. Translational mouse models are a valuable tool for medical research, including research on respiratory viral infections. In in vivo mouse models, synthetic dsRNA can be used...
View PublicationDual RNA sequencing of group B Streptococcus-infected human monocytes reveals new insights into host–pathogen interactions and bacterial evasion of phagocytosis
-
Sullivan MJ, Prince D, Goh KGK, et al. ,
-
Sci Rep ,
-
2023,
-
13(1):2137
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Streptococcus agalactiae, also known as Group B Streptococcus (GBS) is a frequent cause of infections, including bacteraemia and other acute diseases in adults and immunocompromised individuals. We developed a novel system to study GBS within human monocytes to define the...
View PublicationIn Vitro Evaluation of Antiprotozoal Properties, Cytotoxicity Effect and Anticancer Activity of New Essential-Oil Based Phytoncide Mixtures
-
Iwiński H, Różański H, Pachura N, et al. ,
-
Molecules ,
-
2023,
-
28(3):1395
- Instrument : NucleoCounter® NC-200™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Protozoa, in both humans and animals, are one of the leading causes of disease. International programmes introduced in many countries have helped reduce the incidence of disease. However, it has recently become increasingly difficult to achieve the goals set for...
View PublicationEngineering 3D micro-compartments for highly efficient and scale-independent expansion of human pluripotent stem cells in bioreactors
-
Cohen PJR, Luquet E, Pletenka J, et al. ,
-
Biomaterials ,
-
2023,
-
295:122033
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Human pluripotent stem cells (hPSCs) have emerged as the most promising cellular source for cell therapies. To overcome the scale-up limitations of classical 2D culture systems, suspension cultures have been developed to meet the need for large-scale culture in regenerative...
View PublicationCharacterization of large extracellular vesicles (L-EV) derived from human regulatory macrophages (Mreg): novel mediators in wound healing and angiogenesis?
-
Albrecht M, Hummitzsch L, Rusch R, et al. ,
-
J Transl Med ,
-
2023,
-
21(1):61
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Large extracellular vesicles (L-EV) with a diameter between 1 and 10 µm are released by various cell types. L-EV contain and transport active molecules which are crucially involved in cell to cell communication. We have shown that secretory products...
View PublicationB-cell cytokine responses to Porphyromonas gingivalis in patients with periodontitis and healthy controls
-
Danielsen AK, Damgaard C, Massarenti L, et al. ,
-
J Periodontol ,
-
2023,
-
- Instrument : NucleoCounter® NC-200™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell-based immunotherapy
Background: Cytokine-producing B cells play a well-established role in modifying immune responses in chronic inflammatory diseases. We characterized B-cell cytokine responses against periodontitis-associated bacteria in patients with periodontitis. Methods: Blood and saliva samples were collected from patients with periodontitis grade...
View PublicationPoly(ethylene oxide)/Gelatin-Based Biphasic Photocrosslinkable Hydrogels of Tunable Morphology for Hepatic Progenitor Cell Encapsulation
-
Meng Y, Gantier M, Nguyen TH, et al. ,
-
Biomacromolecules ,
-
2023,
-
24(2):789-796
- Instrument : NucleoCounter® NC-200™
- Cell Type: Hepatocytes
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Macroporous hydrogels have great potential for biomedical applications. Liquid or gel-like pores were created in a photopolymerizable hydrogel by forming water-in-water emulsions upon mixing aqueous solutions of gelatin and a poly(ethylene oxide) (PEO)-based triblock copolymer. The copolymer constituted the continuous...
View PublicationDevelopment and scale-up of rVSV-SARS-CoV-2 vaccine process using single use bioreactor
-
Ton C, Stabile V, Carey E, et al. ,
-
Biotechnol Rep (Amst) ,
-
2023,
-
37:e00782
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Virus Production
The outbreak of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes the Coronavirus Disease 2019 (COVID-19) has spread through the globe at an alarming speed. The disease has become a global pandemic affecting millions of people and...
View PublicationBenzophenone-3 exposure alters composition of tumor infiltrating immune cells and increases lung seeding of 4T1 breast cancer cells
-
Morin SM, Gregory KJ, Medeiros B, et al. ,
-
Advances in Cancer Biology - Metastasis ,
-
2023,
-
7:100080
- Instrument : NucleoCounter® NC-202™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Environmental chemicals are a persistent and pervasive part of everyday life. A subset of environmental chemicals are xenoestrogens, compounds that bind to the estrogen receptor (ER) and drive estrogen-related processes. One such chemical, benzophenone-3 (BP3), is a common chemical in...
View Publication3D Microcapsules for Human Bone Marrow Derived Mesenchymal Stem Cell Biomanufacturing in a Vertical-Wheel Bioreactor
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Teryek M, Jadhav P, Bento R, Parekkadan B ,
-
bioRxiv ,
-
2023,
-
- Instrument : NucleoCounter® NC-202™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Microencapsulation of human mesenchymal stromal cells (MSCs) via electrospraying has been well documented in tissue engineering and regenerative medicine. Herein, we report the use of microencapsulation, via electrospraying, for MSC expansion using a commercially available hydrogel that is durable, optimized...
View Publication2022
Designing and executing prime editing experiments in mammalian cells
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Doman JL, Sousa AA, Randolph PB, et al. ,
-
Nat Protoc ,
-
2022,
-
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Gene Therapy
Prime editing (PE) is a precision gene editing technology that enables the programmable installation of substitutions, insertions and deletions in cells and animals without requiring double-strand DNA breaks (DSBs). The mechanism of PE makes it less dependent on cellular replication...
View PublicationNeedle to needle robot-assisted manufacture of cell therapy products
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Ochs J, Hanga MP, Shaw G, et al. ,
-
Bioeng Transl Med ,
-
2022,
-
e10387
- Instrument : NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
Advanced therapeutic medicinal products (ATMPs) have emerged as novel therapies for untreatable diseases, generating the need for large volumes of high-quality, clinically-compliant GMP cells to replace costly, high-risk and limited scale manual expansion processes. We present the design of a...
View PublicationIn vitro toxicological assessment of PhSeZnCl in human liver cells
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di Vito R, Levorato S, Fatigoni C, et al. ,
-
Toxicological Research ,
-
2022,
-
01/Oct
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Oncology
Phenylselenenylzinc chloride (PhSeZnCl) is an air-stable selenolate, easily synthesizable through oxidative insertion of elemental zinc into the Se-halogen bond of the commercially available phenylselenyl chloride. PhSeZnCl was shown to possess a marked GPx-like activity both in NMR and in vitro...
View PublicationComparative roadmaps of reprogramming and oncogenic transformation identify Bcl11b and Atoh8 as broad regulators of cellular plasticity
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Huyghe A, Furlan G, Schroeder J, et al. ,
-
Nat Cell Biol ,
-
2022,
-
24(9):1350-1363
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Coordinated changes of cellular plasticity and identity are critical for pluripotent reprogramming and oncogenic transformation. However, the sequences of events that orchestrate these intermingled modifications have never been comparatively dissected. Here, we deconvolute the cellular trajectories of reprogramming (via Oct4/Sox2/Klf4/c-Myc)...
View PublicationInteraction between moxifloxacin and Mcl-1 and MITF proteins: the effect on growth inhibition and apoptosis in MDA-MB-231 human triple-negative breast cancer cells
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Beberok A, Rok J, Rzepka Z, et al. ,
-
Pharmacol Rep ,
-
2022,
-
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Vitality Assay
- Research area: Oncology
Background: Microphthalmia-associated transcription factor (MITF) activates the expression of genes involved in cellular proliferation, DNA replication, and repair, whereas Mcl-1 is a member of the Bcl-2 family of proteins that promotes cell survival by preventing apoptosis. The objective of the...
View PublicationHnRNPK maintains single strand RNA through controlling double-strand RNA in mammalian cells
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Mahale S, Setia M, Prajapati B, et al. ,
-
Nat Commun ,
-
2022,
-
13(1):4865
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay
- Research area: Cell Biology
Although antisense transcription is a widespread event in the mammalian genome, double-stranded RNA (dsRNA) formation between sense and antisense transcripts is very rare and mechanisms that control dsRNA remain unknown. By characterizing the FGF-2 regulated transcriptome in normal and cancer...
View PublicationThe effects of co-exposure to methyl paraben and dibutyl phthalate on cell line derived from human skin
-
Miranowicz-Dzierżawska K, Zapór L, Skowroń J, et al. ,
-
Toxicological Research ,
-
2022,
-
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Caspase 3/7 or 8 or 9 Assay
- Research area: Oncology
Data on the cumulative effects of chemical substances are necessary for the proper risk assessment, but their availability is still insufficient. The aim of the study was to evaluate the cytotoxic effect of methyl paraben (MePB) and dibutyl phthalate (DBP)...
View PublicationEstablishment of In Vitro Models by Stress-Induced Premature Senescence for Characterizing the Stromal Vascular Niche in Human Adipose Tissue
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Wahlmueller M, Wahlmueller MS, Missfeldt K, et al. ,
-
Life ,
-
2022,
-
12, 1459
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Cycle Assay
- Research area: Stem Cell Research
Acting as the largest energy reservoir in the body, adipose tissue is involved in longevity and progression of age-related metabolic dysfunction. Here, cellular senescence plays a central role in the generation of a pro-inflammatory environment and in the evolution of...
View PublicationEvaluation of 3,5-Diphenyl-2-Pyrazolines for Antimitotic Activity by Inhibition of Tubulin Polymerization
-
Shin SY, Jung E, Lee YH, et al. ,
-
Journal of Chemistry ,
-
2022,
-
3567606
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Chalcones have a skeleton of diphenyls connected via an α,β-unsaturated carbonyl group. Many chalcone derivatives have been shown to interact with tubulin. Based on previous reports, chalcones derived by substitution of a carbonyl group with 2-pyrazoline can be expected to...
View PublicationAnticancer and antimicrobial activity of new copper (II) complexes
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Climova A, Pivovarova E, Szczesio M, et al. ,
-
J Inorg Biochem ,
-
2022,
-
240:112108
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Epithelial Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Cell Biology
In this study, three new organic ligands N′-(benzylidene)-6-chloropyrazine-2-carbohydrazonamide (L1), 6-chloro-N′-(4-nitrobenzylidene)picolinohydrazonamide(L2), and N′-(benzylidene)-4-chloropicolinohydrazonamide (L3) and three copper coordination compounds (Cu(L1)Cl2, Cu(L2)Cl2 and Cu(L3)Cl2) based on them were synthesized. All obtained compounds were characterized using appropriate analytical techniques: elemental analysis (EA), thermogravimetric...
View PublicationImpact of alcohol exposure on neural development and network formation in human cortical organoids
-
Adams JW, Negraes PD, Truong J, et al. ,
-
Mol Psychiatry ,
-
2022,
-
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Neural Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Neurobiology
Prenatal alcohol exposure is the foremost preventable etiology of intellectual disability and leads to a collection of diagnoses known as Fetal Alcohol Spectrum Disorders (FASD). Alcohol (EtOH) impacts diverse neural cell types and activity, but the precise functional pathophysiological effects...
View PublicationCryopreservation does not change the performance and characteristics of allogenic mesenchymal stem cells highly over-expressing a cytoplasmic therapeutic transgene for cancer treatment
-
Ho YK, Loke KM, Woo JY, et al. ,
-
Stem Cell Res Ther ,
-
2022,
-
0,902083333
- Instrument : NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
Background Mesenchymal stem cells (MSCs) driven gene directed enzyme prodrug therapy is a promising approach to deliver therapeutic agents to target heterogenous solid tumours. To democratize such a therapy, cryopreservation along with cold chain transportation is an essential part of...
View PublicationMolecular Characterization of Secreted Factors and Extracellular Vesicles-Embedded miRNAs from Bone Marrow-Derived Mesenchymal Stromal Cells in Presence of Synovial Fluid from Osteoarthritis Patients
-
Ragni E, Perucca Orfei C, Valli F, et al. ,
-
Biology (Basel) ,
-
2022,
-
11(11):1632
- Instrument : NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Bone marrow-derived mesenchymal stromal cells (BMSCs)-based therapies show a great potential to manage inflammation and tissue degeneration in osteoarthritis (OA) patients. Clinical trials showed the ability to manage pain and activation of immune cells and allowed restoration of damaged cartilage....
View PublicationSynthesis and Anticancer Activity of Indole-Functionalized Derivatives of Betulin
-
Rzepka Z, Bębenek E, Chrobak E, Wrześniok D ,
-
Pharmaceutics ,
-
2022,
-
14(11):2372
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, DNA Fragmentation Assay
- Research area: Oncology
Pentacyclic triterpenes, including betulin, are widespread natural products with various pharmacological effects. These compounds are the starting material for the synthesis of substances with promising anticancer activity. The chemical modification of the betulin scaffold that was carried out as part...
View PublicationDisruption of mitochondrial function augments the radiosensitivity of prostate cancer cell lines
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Adamczuk GM, Humeniuk E, Adamczuk K, et al. ,
-
Ann Agric Environ Med ,
-
2022,
-
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Oncology
Introduction: Ionizing radiation is one of the most widely used therapeutic methods in the treatment of prostate cancer, but the problem is developing radioresistance of the tumour. There is evidence that metabolic reprogramming in cancer is one of the major...
View PublicationNew Betulin Derivatives with Nitrogen Heterocyclic Moiety—Synthesis and Anticancer Activity In Vitro
-
Bębenek E, Chrobak E, Rzepka Z, Wrześniok D ,
-
Biomolecules ,
-
2022,
-
12(10):1540
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay, DNA Fragmentation Assay
- Research area: Oncology
As part of the search for new medicinal substances with potential application in oncology, the synthesis of new compounds combining the betulin molecule and the indole system was carried out. The structure of the ester derivatives obtained in the Steglich...
View PublicationEarly intervention with 3BNC117 and romidepsin at antiretroviral treatment initiation in people with HIV-1: a phase 1b/2a, randomized trial
-
Gunst JD, Pahus MH, Rosás-Umbert M, et al. ,
-
Nat Med ,
-
2022,
-
28(11):2424-2435
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
Attempts to reduce the human immunodeficiency virus type 1 (HIV-1) reservoir and induce antiretroviral therapy (ART)-free virologic control have largely been unsuccessful. In this phase 1b/2a, open-label, randomized controlled trial using a four-group factorial design, we investigated whether early intervention...
View PublicationInfluences of repeated exposure to low levels of TiO2 nanoparticles on Kruppel-like factors in 3D Caco-2 spheroids and mouse intestines
-
Cao W, Liu M, Wang C, et al. ,
-
J Appl Toxicol ,
-
2022,
-
- Instrument : NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Kruppel-like factors (KLFs) are a set of transcription factors (TFs) involved in the regulation of many basic biological processes, and recent studies suggested that nanoparticles (NPs) were capable to change KLFs in different models even at non-cytotoxic concentrations. In this...
View PublicationRapid Antibody Glycoengineering in CHO Cells Via RNA Interference and CGE-LIF N-Glycomics
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Kotidis P, Marbiah M, Donini R, et al. ,
-
Methods Mol Biol ,
-
2022,
-
2370:147-167
- Instrument : NucleoCounter® NC-250™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Graft-contaminating tumor cells correlate with inferior outcome in high-risk neuroblastoma patients undergoing hematopoietic stem cell transplantation and can contribute to relapse. Motivated by the potential therapeutic benefit of tumor cell removal as well as the high prognostic and diagnostic...
View PublicationCharacterization of the Antibacterial Activity of an SiO 2 Nanoparticular Coating to Prevent Bacterial Contamination in Blood Products
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Fonseca S, Cayer MP, Ahmmed KMT, et al. ,
-
Antibiotics ,
-
2022,
-
11(1):107
- Instrument : NucleoCounter® NC-250™
- Cell Type: Whole Blood
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Technological innovations and quality control processes within blood supply organizations have significantly improved blood safety for both donors and recipients. Nevertheless, the risk of transfusion-transmitted infection remains non-negligible. Applying a nanoparticular, antibacterial coating at the surface of medical devices is...
View PublicationSoft-sensors application for automated feeding control in high-throughput mammalian cell cultures
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Martínez-Monge I, Martínez C, Decker M, et al. ,
-
Biotechnol Bioeng ,
-
2022,
- Instrument : NucleoCounter® NC-250™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The ever-increasing demand for biopharmaceuticals has created the need for improving the overall productivity of culture processes. One such operational concept that is considered is fed-batch operations as opposed to batch operations. However, optimal fed-batch operations require complete knowledge of...
View PublicationTherapeutic Potential of Pretreatment with Exosomes Derived from Stem Cells from the Apical Papilla against Cisplatin-Induced Acute Kidney Injury
-
Huang TY, Chien MS, Su WT ,
-
Int J Mol Sci ,
-
2022,
-
23(10):5721
- Instrument : NucleoCounter® NC-250™
- Cell Type: Epithelial Cells
- Assay: Cell Vitality Assay
- Research area: Stem Cell Research
Acute kidney injury (AKI) is the most serious side effect of treatment with cisplatin in clinical practice. The aim of this study was to investigate the therapeutic effect of exosomes derived from stem cells from the apical papilla (SCAPs) on...
View PublicationPolyhexamethylene guanidine phosphate-induced necrosis may be linked to pulmonary fibrosis
-
Kang MS, Kim SH, Yang MJ, et al. ,
-
Toxicol Lett ,
-
2022,
-
362:1-16
- Instrument : NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Following the humidifier disinfectant incident in Korea, polyhexamethylene guanidine phosphate (PHMG-P) has been used to establish lung fibrosis model animals. Herein, we investigated time-dependent changes after a single PHMG-P instillation (22 μg/lung) to identify the underlying pathogenesis and immune response...
View PublicationComprehensive Targeted Metabolomic Study in the Lung, Plasma, and Urine of PPE/LPS-Induced COPD Mice Model
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Kim HY, Lee HS, Kim IH, et al. ,
-
Int J Mol Sci ,
-
2022,
-
23(5):2748
- Instrument : NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
(1) Background: Progression of chronic obstructive pulmonary disease (COPD) leads to irreversible lung damage and inflammatory responses; however, biomarker discovery for monitoring of COPD progression remains challenging. (2) Methods: This study evaluated the metabolic mechanisms and potential biomarkers of COPD...
View PublicationSublethal pulmonary toxicity screening of silica nanoparticles in rats after direct intratracheal instillation
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Han HY ,
-
Toxicological Research ,
-
2022,
- Instrument : NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The present aimed to characterize the toxicity of silica nanoparticles in Sprague Dawley rats and determine the dose levels for a repeated-dose toxicity study. Silica nanoparticles (SiO2, 20 nm and 50 nm) were administered as a single intratracheal instillation of...
View PublicationSARS-CoV2 wild type and mutant specific humoral and T cell immunity is superior after vaccination than after natural infection
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Richardson JR, Götz R, Mayr V, et al. ,
-
PLoS One ,
-
2022,
-
17(4):e0266701
- Instrument : NucleoCounter® NC-250™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Objective: We investigated blood samples from fully SARS-CoV2-vaccinated subjects and from previously positive tested patients up to one year after infection with SARS-CoV2, and compared short- and long-term T cell and antibody responses, with a special focus on the recently...
View PublicationDNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus)
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Jeuthe H, Palaiokostas C, Johannisson A ,
-
Aquaculture, ,
-
2022,
-
547:737537
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
This experiment was conducted to assess the effect of different antibiotics in tris-fructose egg yolk-based diluent on bacterial load and sperm quality of dromedary camels during processing and cold storage. Ten semen ejaculates were collected from five male dromedary camels....
View PublicationDisruption of Membrane Integrity as a Molecular Initiating Event Determines the Toxicity of Polyhexamethylene Guanidine Phosphate Depending on the Routes of Exposure
-
Song J, Jung KJ, Yang MJ, et al. ,
-
Int J Mol Sci ,
-
2022,
-
23(6):3289
- Instrument : NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Polyhexamethylene guanidine phosphate (PHMG-P), a cationic biocide, is widely used in household products due to its strong bactericidal activity and low toxicity. However, it causes fatal lung damage when inhaled. In this study, we investigated why PHMG-P causes fatal lung...
View PublicationToward commercialization: Improvement of a semen cryopreservation protocol for European perch enables its implementation for commercial-scale fertilization
-
Judycka S, Żarski D, Dietrich MA, et al. ,
-
Aquaculture ,
-
2022,
-
549(737790)
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
This study emphasis improved semen cryopreservation protocol for commercial scale fertilization in European perch. The optimized procedure involves a new extender (GM 25 K) containing 0.30 M glucose, 7.5% methanol and 25 mM KCl at 3.0 × 109/mL spermatozoa. The...
View PublicationThe effects of air pollutants exposure on the transmission and severity of invasive infection caused by an opportunistic pathogen Streptococcus pyogenes
-
Zhi Y, Chen X, Cao G, et al. ,
-
Environ Pollut ,
-
2022,
-
119826
- Instrument : NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Currently, urbanization is associated with an increase in air pollutants that contribute to invasive pathogen infections by altering the host’s innate immunity and antimicrobial resistance capability. Streptococcus pyogenes, also known as Group A Streptococcus (GAS), is a gram-positive opportunistic pathogen...
View PublicationImpact of glyphosate and its formulation Roundup® on stallion spermatozoa
-
Spinaci M, Nerozzi C, Mislei B, et al. ,
-
Theriogenology ,
-
2022,
-
179:197-203
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
The growing and widespread use of glyphosate-based herbicides (GBHs) has raised an intense public debate about the impact of environmental contamination on animal and human health, including male fertility. The aim of this study was to deepen the impact of...
View PublicationSodium dichloroisocyanurate toxicity in rats during a 90-day inhalation toxicity study
-
Park CM, Jeon S, Kim YH, et al. ,
-
Toxicol Appl Pharmacol ,
-
2022,
-
116279
- Instrument : NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Sodium dichloroisocyanurate-96% (NaDCC) is commonly used to treat drinking water, industrial water, and wastewater. However, exposure to NaDCC by inhalation can have toxic pulmonary effects in humans. In the present study, we evaluated the potential toxicity of NaDCC following a...
View PublicationMicrobiological and physicochemical properties of farm bulk tank milk and antimicrobial resistance of its dominant bacteria
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Ozdikmenli ST, Zorba M, Yalman M, et al. ,
-
Journal of Food Safety ,
-
2022,
-
- Instrument : NucleoCounter® SCC-100™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Dairy (SCC)
This study determined the antibiotic resistance of the dominant bacteria in the 85 farm BTMs according to the guidelines recommended by the epidemiological cutoff values in the EUCAST. In addition, some physicochemical and microbiological properties of farm BTMs were investigated....
View PublicationPractical Method for Freezing Buck Semen
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Morrell JM, Malaluang P, Ntallaris T, Johannisson A ,
-
Animals ,
-
2022,
-
12(3):352
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Although several protocols for cryopreserving buck semen are described in the literature, they differ widely in factors such as season and method of semen collection, extender and sperm concentration. Therefore, choosing a protocol that is suitable for a particular on-farm...
View PublicationBovine Papillomavirus Type 1 or 2 Virion-Infected Primary Fibroblasts Constitute a Near-Natural Equine Sarcoid Model
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Hainisch EK, Jindra C, Reicher P, et al. ,
-
Viruses ,
-
2022,
-
14(12):2658
- Instrument : NucleoCounter® NC-250™
- Cell Type: Primary Cells
- Assay: Cell Count & Viability Assay
- Research area: Virus Production
Equine sarcoids are common, locally aggressive skin tumors induced by bovine papillomavirus types 1, 2, and possibly 13 (BPV1, BPV2, BPV13). Current in vitro models do not mimic de novo infection. We established primary fibroblasts from horse skin and succeeded...
View PublicationEvaluating the potential of improving sperm quality traits in farmed Arctic charr (Salvelinus alpinus) using selective breeding
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Kurta K, Jeuthe H, de Koning DJ, Palaiokostas C ,
-
Aquaculture Reports ,
-
2022,
-
25(101234)
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Toward commercialization: Improvement of a semen cryopreservation protocol for European perch enables its implementation for commercial-scale fertilization
View PublicationNo increase in pregnancy rate of mares after preovulatory deep uterine horn application of misoprostol
-
Donatsch L, Friker B, Sieme H, et al. ,
-
Theriogenology ,
-
2022,
-
184:132-139
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
A potential source of fertility loss in mares is oviductal dysfunction, potentially caused by masses or debris in the lumen, that may prevent either sperm from reaching the fertilization site or the embryo from reaching the uterus. Recently a novel...
View PublicationSperm Gone Smart: A Portable Device (iSperm®) to Assess Semen Concentration and Motility in Dogs
-
Domain G, Banchi P, Ali Hassan H, et al. ,
-
Animals (Basel) ,
-
2022,
-
12(5):652
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
The iSperm® is a portable device for semen analysis. This study aimed to investigate its correlation with a conventional computer-assisted sperm analyzer (ISAS®v1) for the assessment of semen concentration and kinematic parameters in dogs (n = 224). The intra-assay variability...
View PublicationAllele-Specific Disruption of a Common STAT3 Autosomal Dominant Allele Is Not Sufficient to Restore Downstream Signaling in Patient-Derived T Cells
-
König S, Fliegauf M, Rhiel M, et al. ,
-
Genes (Basel) ,
-
2022,
-
13(10):1912
- Instrument : NucleoCounter® NC-250™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Gene Therapy
Dominant negative mutations in the STAT3 gene account for autosomal dominant hyper-IgE syndrome (AD-HIES). Patients typically present high IgE serum levels, recurrent infections, and soft tissue abnormalities. While current therapies focus on alleviating the symptoms, hematopoietic stem cell transplantation (HSCT)...
View PublicationThe Proteomic Modification of Buck Ejaculated Sperm Induced by the Cryopreservation Process
-
Lv C, Liang J, Yang H, et al. ,
-
Biopreserv Biobank ,
-
2022,
-
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Using two-dimensional electrophoresis along with mass spectroscopy, we have investigated how the cryopreservation process affected the protein profile of goat ejaculated sperm. In this study, five bucks were used for semen collection. After removal of seminal plasma, the Tris-based extender...
View PublicationComparison of toxicity and cellular responses following pulmonary exposure to different types of nanofibers
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Kang MS, Lee GH, Yang MJ, et al. ,
-
Nanotoxicology ,
-
2022,
-
16(9-10):935-954
- Instrument : NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based immunotherapy
Pulmonary effects of inhaled microfibers are an emerging public health concern. In this study, we investigated toxicity following pulmonary exposure to synthetic polyethylene oxide fibroin (PEONF) and silk fibroin (SFNF) nanofibers and the cellular responses. When instilled intratracheally weekly for...
View PublicationInfluence of Single Layer Centrifugation with Canicoll on Semen Freezability in Dogs
-
Domain G, Ali Hassan H, Wydooghe E, et al. ,
-
Animals (Basel) ,
-
2022,
-
12(6):714
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
This study evaluated how semen selection by single layer centrifugation (SLC) with Canicoll affects semen freezability in dogs. A total of eighteen ejaculates, collected from dogs with optimal and suboptimal semen quality (optimal: normal morphology (NM) ≥ 80%, n =...
View PublicationUse of specific mitochondrial complex inhibitors to investigate mitochondrial involvement on horse sperm motility and ROS production
-
Giaretta E, Mislei B, Martínez-Pastor F, et al. ,
-
Res Vet Sci ,
-
2022,
-
147:12-19
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Equine spermatozoa highly rely on oxidative phosphorylation for their energy management. The present work aimed to characterize the role of mitochondria on horse sperm motility and ROS production by incubating spermatozoa with specific inhibitors of the different mitochondrial complexes. Equine...
View PublicationLive cells are not affected by dead sperm in liquid boar semen: New insights based on a thermo-resistance test
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Quirino M, Jakop U, Mellagi APG, et al. ,
-
Reprod Dom Anim ,
-
2022,
-
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
This study evaluated the effect of different proportions of dead spermatozoa on the quality of liquid boar semen during a thermo-resistance test (TRT). After 3 days of storage (17°C), 54 conventional artificial insemination semen doses (~23 × 106 sperm/ml in ~88 ml of BTS)...
View PublicationComparison of NUCLEOCOUNTER, ANDROVISION with Leja chambers and the newly developed ANDROVISION eFlow for sperm concentration analysis in boars
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Grossfeld R, Pable J, Jakop U, et al. ,
-
Sci Rep ,
-
2022,
-
12(1):11943
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Exact analysis of sperm concentration in raw and diluted semen is of major importance in swine artificial insemination, as sperm concentration is one of the most important characteristics of an ejaculate determining the value of the ejaculate and the productive...
View PublicationThe establishment of goat semen protein profile using a tandem mass tag-based proteomics approach
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Liang J, Lv C, Xiang D, et al. ,
-
Res Vet Sci ,
-
2022,
-
150:22-32
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
In this study, the complete proteome of goat ejaculated semen including spermatozoa and seminal plasma was established, applying a tandem mass tag (TMT) labeling together with liquid chromatography-tandem mass spectrometry (LC-MS/MS). In seminal plasma, 2299 proteins were identified and 2098...
View PublicationAn optimized centrifugation protocol for ram sperm ensuring high sample yield, quality and fertility
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Neila-Montero M, Riesco MF, Montes-Garrido R, et al. ,
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Theriogenology ,
-
2022,
-
191:179-191
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
The optimization and implementation of artificial insemination (AI) in sheep is necessary to increase the livestock productivity through enhanced control of reproductive function. Sperm centrifugation is a common procedure in the ejaculate handling in AI and other assisted reproductive technologies...
View PublicationCharacterization of sperm cell membrane charge and selection of high-quality sperm using microfluidics in stallions
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Orsolini MF, Verstraete MH, van Heule M, et al. ,
-
Theriogenology ,
-
2022,
-
192:1-8
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Intracytoplasmic sperm injection (ICSI) is the only method for in vitro embryo production (IVP) in horses. Besides oocyte developmental competence, the outcome of IVP is also highly dependent on sperm quality. Therefore, it is not only essential to employ superior...
View PublicationHydroxytyrosol and resveratrol improves kinetic and flow cytometric parameters of cryopreserved bull semen with low cryotolerance
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Sharafi M, Blondin P, Vincent P, et al. ,
-
Anim Reprod Sci ,
-
2022,
-
245:107065
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
There is considerable interest in breeding programs to “rescue” semen with poor post-thaw fertility from bulls known as “bad freezers”. We hypothesized that there may be an interaction between the post-thaw recovery of sperm and the efficacy of antioxidant addition...
View PublicationImaging flow cytometry to characterize the relationship between abnormal sperm morphologies and reactive oxygen species in stallion sperm
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Bulkeley E, Santistevan AC, Varner D, Meyers S ,
-
Reprod Domest Anim ,
-
2022,
-
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Low levels of intracellular reactive oxygen species (ROS) are essential for normal sperm function and are produced by sperm mitochondria as a byproduct of metabolism, but in excess, ROS can cause catastrophic cellular damage and has been correlated with infertility,...
View PublicationBacterial killing activity and lysozymes: A stable defence mechanism in stallion seminal plasma?
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Jakop U, Hensel B, Czirják GÁ, et al. ,
-
Reprod Domest Anim ,
-
2022,
-
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
During insemination, bacterial contamination of the ejaculate can lead to reduced sperm quality and transmission of pathogens to the female, thus should be avoided. The semen of a variety of animal taxa possess antimicrobial properties against a wide range of...
View PublicationInteraction between moxifloxacin and Mcl-1 and MITF proteins: the effect on growth inhibition and apoptosis in MDA-MB-231 human triple-negative breast cancer cells
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Beberok A, Rok J, Rzepka Z, et al. ,
-
Pharmacol Rep ,
-
2022,
-
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Background: Microphthalmia-associated transcription factor (MITF) activates the expression of genes involved in cellular proliferation, DNA replication, and repair, whereas Mcl-1 is a member of the Bcl-2 family of proteins that promotes cell survival by preventing apoptosis. The objective of the...
View PublicationA 5-color flow cytometry panel to assess plasma membrane integrity, acrosomal status, membrane lipid organization and mitochondrial activity of boar and stallion spermatozoa following liquid semen storage
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Quirino M, Jakop U, Mellagi APG, et al. ,
-
Animal Reproduction Science ,
-
2022,
-
107076
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
For a more practically applicable analysis of different sperm characteristics, this study aimed to develop a 5-color flow cytometry (FC) panel to concurrently analyze four sperm parameters in liquid boar and stallion semen, using also a DNA-marker for selecting sperm...
View PublicationSlow Cooling is Beneficial for Storage of Frozen-Thawed Equine Spermatozoa
-
van Heule M, Verstraete M, Blockx Z, et al. ,
-
J Equine Vet Sci ,
-
2022,
-
118:104132
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Cooled storage of semen after thawing can expand the use of frozen semen, providing the possibility of thawing and evaluating the semen at the storage site and subsequently shipping the semen. Our objectives were (1) to examine the motility and...
View PublicationRe-stimulation of testicular function in GnRH-vaccinated stallions by daily GnRH agonist treatment
-
Gautier C, Aurich J, Kaps M, et al. ,
-
Theriogenology ,
-
2022,
-
194:27-34
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
In stallions temporarily not intended for breeding, reversible suppression of testicular function by vaccination against GnRH can be of interest. In the present study, effects of GnRH agonist treatment on the resumption of testicular function after GnRH vaccination were investigated....
View PublicationSelection of frozen–thawed stallion semen by microfluidic technology
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Vigolo V, Gautier C, Falomo ME, Aurich C ,
-
Reprod Domest Anim ,
-
2022,
-
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
The use of microfluidic technology is increasing in artificial reproduction technologies: With a small amount of semen, it allows for the selection of sperm with the best characteristics of kinetics, morphology and chromatin integrity. The ZyMot Multi (850 μl) is...
View PublicationThe effect of gossypol acetate on the fertility of boars
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Torres M, Balogun K, Shirley L, et al. ,
-
Acta Agraria Debreceniensis ,
-
2022,
-
(2), 47–55
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
This study evaluated the effect of gossypol acetate, a potential antifertility compound, on semen quality and testicular histopathology of boars. Six boars were allotted into two experimental groups. Half of the animals were fed a diet that was supplemented with...
View PublicationSerum Anti-Müllerian Hormone: A Potential Semen Quality Biomarker in Stud Dogs?
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Domain G, Buczkowska J, Kalak P, et al. ,
-
Animals (Basel) ,
-
2022,
-
12(3):323
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Anti-Müllerian hormone (AMH) has been suggested to be involved in spermatogenesis. The aim of this study was to investigate the relationship between blood serum AMH concentration and semen quality in dogs. Moreover, this study sought to find the optimal cut-off...
View PublicationSperm concentration of frozen semen has a leading role in fertility
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Vigolo V, Gautier C, Aurich C, Falomo ME ,
-
Animal Reproduction Science ,
-
2022,
-
247:107143
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
The maintenance of stallion fertility when using frozen semen is one of the main issues in equine reproductive technology. The aim of the present study was to compare the characteristics of frozen semen from good and poor fertility stallions and...
View PublicationKaempferol modulates the expression patterns of heat shock and apoptotic proteins in bovine cryopreserved spermatozoa
-
Tvrda E, Banas S, Benko F, et al. ,
-
Animal Reproduction Science ,
-
2022,
-
247:107142
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
An appealing strategy to overcome cryodamage of frozen spermatozoa is supplementation of biologically active substances exhibiting membrane-stabilizing, motility-promoting, and antioxidant effects. Out of these, kaempferol (KAE), a flavonol molecule has shown promise in prevention of alterations to mitochondrial metabolism and...
View PublicationSucrose and bovine serum albumin in association with dimethylformamide improved sperm parameters of frozen stallion semen
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Kurpita M, Len J ,
-
Clinical Theriogenology ,
-
2022,
-
14(4), 356-361
- Instrument : NucleoCounter® SP-100™
- Cell Type: Sperm cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Postthaw stallion sperm integrity was determined after freezing semen in an extender supplemented with several sucrose concentrations, bovine serum albumin (BSA), and with and without dimethylformamide (DMF). Two ejaculates from 6 stallions (n =12) were diluted and aliquots (n =...
View PublicationPreparation and growth factor characterization of cord blood-derived plasma, serum, growth factor-rich plasma and induced serum
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Rhéaume ME, Perreault J, Fournier D, Trépanier P ,
-
Cytokine ,
-
2022,
-
149:155756
- Instrument : NucleoCounter® NC-200™
- Cell Type: Whole Blood
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Cultured meat is an emergent technology with the potential for significant environmental and animal welfare benefits. Accurate mimicry of traditional meat requires fat tissue; a key contributor to both the flavour and texture of meat. Here, we show that fibro-adipogenic...
View PublicationMuscle-derived fibro-adipogenic progenitor cells for production of cultured bovine adipose tissue
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Dohmen RGJ, Hubalek S, Melke J, et al. ,
-
NPJ Sci Food ,
-
2022,
-
6(1):6
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cultured Meat
Background and aims: Autologous keratinocyte sheets constitute an important component of the burn wound treatment toolbox available to a surgeon and can be considered a life-saving procedure for patients with severe burns over 50% of their total body surface area....
View PublicationOptimizing large-scale autologous human keratinocyte sheets for major burns—Toward an animal-free production and a more accessible clinical application
-
Frese L, Darwiche SE, Gunning ME, et al. ,
-
Health Sci Rep ,
-
2022,
-
5(1):e449
- Instrument : NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Nickel oxide nanoparticles (NiO NPs) are highly redox active nanoparticles. They can cause acute and chronic inflammation in rat lungs. Unlike the gut microbiome, the association between the lung microbiome’s role and pulmonary inflammatory response to inhaled nanoparticles remains largely...
View PublicationExposure to Nickel Oxide Nanoparticles Induces Acute and Chronic Inflammatory Responses in Rat Lungs and Perturbs the Lung Microbiome
-
Jeong MJ, Jeon S, Yu HS, et al. ,
-
Int J Environ Res Public Health ,
-
2022,
-
19(1):522
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Three-dimensional (3D)-bioprinted lipoaspirate-derived adipose tissue (LAT) is a potential alternative to lipo-injection for correcting soft-tissue defects. This study investigated the long-term in vivo survival of 3D-bioprinted LAT and its proteomic signature and cellular composition. We performed proteomic and multicolour flow...
View PublicationLong-term in vivo survival of 3D-bioprinted human lipoaspirate-derived adipose tissue: proteomic signature and cellular content
-
Säljö K, Apelgren P, Stridh Orrhult L, et al. ,
-
Adipocyte ,
-
2022,
-
11(1):34-46
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Chimeric antigen receptor T (CAR-T) cell therapy has led to a paradigm change in cancer treatment. To allow widespread use of this therapy, it is important to reduce the costs associated with cells manufacturing whilst ensuring a high-quality production. Online...
View PublicationAn impedimetric immunosensor for the selective detection of CD34+ T-cells in human serum
-
Díaz-Fernández A, Bernalte E, Fernández-Ramos C, et al. ,
-
Sensors and Actuators B: Chemical ,
-
2022,
-
356(131306)
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Cell therapy using multipotential stromal cells (MSCs) is being used in a variety of clinical settings to induce tissue regeneration. Promising results have also been achieved in the therapy of osteoarthritis. MSCs have been demonstrated to be safe (Borakati et...
View PublicationInfiltration of the Hoffa’s fat pad in patients with osteoarthritis of the knee-Results after one year of follow-up
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Labarre KW, Zimmermann G ,
-
Bone Reports ,
-
2022,
-
101168
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Erectile dysfunction is a common complication associated with type 2 diabetes mellitus (T2DM) and after prostatectomy in relation to cancer. The regenerative effect of cultured adipose-derived stem cells (ASCs) for ED therapy has been documented in multiple preclinical trials as...
View PublicationAdipose-Derived Stem Cells from Type 2 Diabetic Rats Retain Positive Effects in a Rat Model of Erectile Dysfunction
-
Quaade ML, Dhumale P, Steffensen SGC, et al. ,
-
International Journal of Molecular Sciences ,
-
2022,
-
23(3):1692
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
The promise of human induced pluripotent stem cells (iPSCs) lies in their ability to serve as a starting material for autologous, or patient-specific, stem cell-based therapies. Since the first publications describing the generation of iPSCs from human tissue in 2007,...
View PublicationValidation of automated fluorescent-based technology for measuring total nucleated cell viability of hematopoietic progenitor cell products
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Kanan A. Patel,Nita Patel,Grace H. Kim, et al. ,
-
Transfusion ,
-
2022,
-
- Instrument : NucleoCounter® NC-200™
- Cell Type: HSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Background A reliable rapid method for measuring total nucleated cell (TNC) viability is essential for cell-based products manufacturing. The trypan blue (TB) exclusion method, commonly used to measure TNC viability of hematopoietic progenitor cell (HPC) products, is a subjective assay,...
View PublicationNovel CD19 chimeric antigen receptor T cells manufactured next-day for acute lymphoblastic leukemia
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Zhang C, He J, Liu L, et al. ,
-
Blood Cancer J ,
-
2022,
-
12(6):96
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Chimeric antigen receptor-engineered T (CAR-T) cells have shown promising efficacy in patients with relapsed/refractory B cell acute lymphoblastic leukemia (R/R B-ALL). However, challenges remain including long manufacturing processes that need to be overcome. We presented the CD19-targeting CAR-T cell product...
View PublicationEnhancing CHO cell productivity through a dual selection system using Aspg and Gs in glutamine free medium
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Ha T, Òdena A, Karottki KJlC, et al. ,
-
Authorea ,
-
2022,
-
- Instrument : NucleoCounter® NC-200™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
The dominant method for generating Chinese hamster ovary (CHO) cell lines that produce high titers of biotherapeutic proteins utilizes selectable markers such as dihydrofolate reductase (Dhfr) or glutamine synthetase (Gs), alongside inhibitory compounds like methotrexate (MTX) or methionine sulfoximine (MSX),...
View PublicationYAP activates pancreatic stellate cells and enhances pancreatic fibrosis
-
Spanehl L, Revskij D, Bannert K, et al. ,
-
Hepatobiliary & Pancreatic Diseases International ,
-
2022,
-
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background Pancreatic stellate cells (PSCs) foster the progression of pancreatic adenocarcinoma and chronic pancreatitis (CP) by producing a dense fibrotic stroma. However, the incomplete knowledge of PSCs biology hampers the exploration of antifibrotic therapies. Here, we explored the role of...
View PublicationFat Grafting With Expanded Adipose-derived Stromal Cells for Breast Augmentation: A Randomized Controlled Trial
-
Vester-Glowinski PV, Herly M, Ørholt M, et al. ,
-
Aesthet Surg J ,
-
2022,
-
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Background The main challenge with fat grafting is that a varying part of the graft is lost to post-surgery resorption. Previous studies suggest that adipose-derived stromal cells (ASCs) can improve the volume retention of fat grafts but there is a...
View PublicationA Platform of Patient-Derived Microtumors Identifies Individual Treatment Responses and Therapeutic Vulnerabilities in Ovarian Cancer
-
Anderle N, Koch A, Gierke B, et al. ,
-
Cancers ,
-
2022,
-
14(12):2895
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
In light of the frequent development of therapeutic resistance in cancer treatment, there is a strong need for personalized model systems representing patient tumor heterogeneity, while enabling parallel drug testing and identification of appropriate treatment responses in individual patients. Using...
View PublicationStandard toxicity study of clinical-grade allogeneic human bone marrow-derived clonal mesenchymal stromal cells
-
Tayebi B, Babaahmadi M, Pakzad M, et al. ,
-
Stem Cell Res Ther ,
-
2022,
-
13(1):213
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Introduction: Mesenchymal stromal cells (MSCs) have opened a new window to treat inflammatory and non-inflammatory diseases. Nonetheless, their clinical applications require rigorous control and monitoring procedures to ensure full compliance with the principles of good manufacturing practice (GMP). Various evaluations...
View PublicationTreatment of Abdominal Aortic Aneurysm Utilizing Adipose-Derived Mesenchymal Stem Cells in a Porcine Model
-
Zilberman B, Kooragayala K, Lou J, et al. ,
-
J Surg Res ,
-
2022,
-
278:247-256
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cardiology
Introduction: The current treatment paradigm of abdominal aortic aneurysms (AAA) focuses on observing patients until their disease reaches certain thresholds for intervention, with no preceding treatment available. There is an opportunity to develop novel therapies to prevent further aneurysmal growth...
View PublicationScalable expansion of iPSC and their derivatives across multiple lineages
-
Kwok CK, Sébastien I, Hariharan K, et al. ,
-
Reprod Toxicol ,
-
2022,
-
112:23-35
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Induced pluripotent stem cell (iPSC) technology enabled the production of pluripotent stem cell lines from somatic cells from a range of known genetic backgrounds. Their ability to differentiate and generate a wide variety of cell types has resulted in their...
View PublicationLarge-scale engineering of hiPSC-derived nephron sheets and cryopreservation of their progenitors
-
Wiersma LE, Avramut MC, Lievers E, et al. ,
-
Stem Cell Res Ther ,
-
2022,
-
13(1):208
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Background: The generation of human induced pluripotent stem cells (hiPSCs) has opened a world of opportunities for stem cell-based therapies in regenerative medicine. Currently, several human kidney organoid protocols are available that generate organoids containing kidney structures. However, these kidney...
View PublicationCD206+ tumor-associated macrophages cross-present tumor antigen and drive antitumor immunity
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Modak M, Mattes AK, Reiss D, et al. ,
-
JCI Insight ,
-
2022,
-
7(11):e155022
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
In many solid cancers, tumor-associated macrophages (TAM) represent the predominant myeloid cell population. Antigen (Ag) cross-presentation leading to tumor Ag-directed cytotoxic CD8+ T cell responses is crucial for antitumor immunity. However, the role of recruited monocyte-derived macrophages, including TAM, as...
View PublicationOptimization of a PDMS-Based Cell Culture Substrate for High-Density Human-Induced Pluripotent Stem Cell Adhesion and Long-Term Differentiation into Cardiomyocytes under a Xeno-Free Condition
-
Etezadi F, Le MNT, Shahsavarani H, et al. ,
-
ACS Biomater Sci Eng ,
-
2022,
-
8(5):2040-2052
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Despite the numerous advantages of PDMS-based substrates in various biomedical applications, they are limited by their highly hydrophobic surface that does not optimally interact with cells for attachment and growth. Hence, the lack of lengthy and straightforward procedures for high-density...
View PublicationExposure of human immune cells, to the antiretrovirals efavirenz and lopinavir, leads to lower glucose uptake and altered bioenergetic cell profiles through interactions with SLC2A1
-
Heaton BJ, Jensen RL, Line J, et al. ,
-
Biomed Pharmacother ,
-
2022,
-
150:112999
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
SLC2A1 mediates glucose cellular uptake; key to appropriate immune function. Our previous work has shown efavirenz and lopinavir exposure inhibits T cell and macrophage responses, to known agonists, likely via interactions with glucose transporters. Using human cell lines as a...
View PublicationStromal Vascular Fraction and Mesenchymal Stem Cells from Human Adipose Tissue: A Comparison of Immune Modulation and Angiogenic Potential
-
Tran TDX, Pham VQ, Tran NN, et al. ,
-
Adv Exp Med Biol ,
-
2022,
-
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Introduction: In recent years, both stromal vascular fraction (SVF) from adipose tissue and mesenchymal stem cells (MSC) from adipose tissues were extensively used in both preclinical and clinical treatment for various diseases. Some studies reported differences in treatment efficacy between...
View PublicationQbD-guided pharmaceutical development of Pembrolizumab biosimilar candidate PSG-024 propelled to industry meeting primary requirements of comparability to Keytruda®
-
Jaffar-Aghaei M, Khanipour F, Maghsoudi A, et al. ,
-
Eur J Pharm Sci ,
-
2022,
-
173:106171
- Instrument : NucleoCounter® NC-200™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
Pharmaceutical development of biosimilars is primarily focused on meeting the regulatory requirements for analytical comparability of the product’s critical quality attributes (CQAs), concerning safety and efficacy, to those of the originator drug of interest. To this end, the early adoption...
View PublicationAnti-CTLA-4 and anti-PD-1 immunotherapies repress tumor progression in preclinical breast and colon model with independent regulatory T cells response
-
Rupp T, Genest L, Babin D, et al. ,
-
Transl Oncol ,
-
2022,
-
20:101405
- Instrument : NucleoCounter® NC-200™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
The recent development of immunotherapy represents a significant breakthrough in cancer therapy. Several immunotherapies provide robust efficacy gains in a wide variety of cancers. However, in some patients the immune checkpoint blockade remains ineffective due to poor therapeutic response and...
View PublicationInhibition of lung microbiota-derived proapoptotic peptides ameliorates acute exacerbation of pulmonary fibrosis
-
D'Alessandro-Gabazza CN, Yasuma T, Kobayashi T, et al. ,
-
Nat Commun ,
-
2022,
-
13(1):1558
- Instrument : NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
Idiopathic pulmonary fibrosis is an incurable disease of unknown etiology. Acute exacerbation of idiopathic pulmonary fibrosis is associated with high mortality. Excessive apoptosis of lung epithelial cells occurs in pulmonary fibrosis acute exacerbation. We recently identified corisin, a proapoptotic peptide...
View PublicationDesign and biofabrication of a leaf-inspired vascularized cell-delivery device
-
Sämfors S, Niemi EM, Oskarsdotter K, et al. ,
-
Bioprinting ,
-
2022,
-
26:e00199
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
We designed and biofabricated a channeled construct as a possible cell-delivery device that can be endothelialized to overcome size limitations due to oxygen diffusion. The channeled device mimicking a leaf was designed using computer-aided design software, with fluid flow through...
View PublicationExtracorporeal immune cell therapy of sepsis: ex vivo results
-
Klinkmann G, Wild T, Heskamp B, et al. ,
-
Intensive Care Med Exp ,
-
2022,
-
10(1):26
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based immunotherapy
Background: Immune cell dysfunction plays a central role in sepsis-associated immune paralysis. The transfusion of healthy donor immune cells, i.e., granulocyte concentrates (GC) potentially induces tissue damage via local effects of neutrophils. Initial clinical trials using standard donor GC in...
View PublicationImmuno-reactive cancer organoid model to assess effects of the microbiome on cancer immunotherapy
-
Shelkey E, Oommen D, Stirling ER, et al. ,
-
Sci Rep ,
-
2022,
-
12(1):9983
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Immune checkpoint blockade (ICB) therapy has demonstrated good efficacy in many cancer types. In cancers such as non-resectable advanced or metastatic triple-negative breast cancer (TNBC), it has recently been approved as a promising treatment. However, clinical data shows overall response...
View PublicationAssociation of heat shock protein 8 with atopic march in a murine experimental model
-
Jeong KT, Do JH, Lee SH, et al. ,
-
PeerJ ,
-
2022,
-
10:e13247
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Atopic march (AM), a unique characteristic of allergic diseases, refers to the sequential progression of atopic dermatitis (AD) in infants to allergic asthma and allergic rhinitis in children and young adults, respectively. Although there are several studies on AM,...
View PublicationFractal Dimension and Texture Analysis in the Assessment of Experimental Laser-Induced Periodic Surface Structures (LIPSS) Dental Implant Surface—In Vitro Study Preliminary Report
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Hadzik J, Kubasiewicz-Ross P, Simka W, et al. ,
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Materials (Basel) ,
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2022,
-
15(8):2713
- Instrument : NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Laser-induced periodic surface structures (LIPSS) are the sub-wavelength periodic nanostructures generated by the femtosecond laser. Implant topography and its nanostructural changes can be important for biomedical applications. In order to compare the surface topography of different implants, appropriate mathematical and...
View PublicationThe telomeric 5′ end nucleotide is regulated in the budding yeast Naumovozyma castellii
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Itriago H, Jaiswal RK, Philipp S, Cohn M ,
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Nucleic Acids Res ,
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2022,
-
50(1):281-292
- Instrument : NucleoCounter® YC-100™
- Cell Type: Yeast Cells
- Assay: Cell Count & Viability Assay
- Research area: Brewing
The junction between the double-stranded and single-stranded telomeric DNA (ds-ss junction) is fundamental in the maintenance of the telomeric chromatin, as it directs the assembly of the telomere binding proteins. In budding yeast, multiple Rap1 proteins bind the telomeric dsDNA,...
View PublicationIn vitro culture at 39 °C during hepatic maturation of human ES cells facilitates hepatocyte-like cell functions
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Imamura S, Yoshimoto K, Terada S, et al. ,
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Sci Rep ,
-
2022,
-
12(1):5155
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Hepatocyte-like cells derived from human pluripotent stem cells (hPSC-HLCs) offer an alternative to primary hepatocytes commonly used for drug screenings and toxicological tests. However, these cells do not have hepatic functions comparable to those of hepatocytes in vivo due to...
View PublicationUtility of Islet Cell Preparations From Donor Pancreases After Euthanasia
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De Paep DL, Van Hulle F, Ling Z, et al. ,
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Cell Transplant ,
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2022,
-
31:9636897221096160
- Instrument : NucleoCounter® YC-100™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Patients fulfilling criteria for euthanasia can choose to donate their organs after circulatory death [donors after euthanasia (DCD V)]. This study assesses the outcome of islet cell isolation from DCD V pancreases. A procedure for DCD V procurement provided 13...
View PublicationMicrowell bag culture for large-scale production of homogeneous islet-like clusters
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Suenaga R, Konagaya S, Yamaura J, et al. ,
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Sci Rep ,
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2022,
-
12(1):5221
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Pluripotent stem-cell derived cells can be used for type I diabetes treatment, but we require at least 105-106 islet-like clusters per patient. Although thousands of uniform cell clusters can be produced using a conventional microwell plate, numerous obstacles need to...
View PublicationPhysiologic and Subjective Health Benefits of Milmed among Racehorses in Training
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Erixon RM, Lenz T, Businaro R ,
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J Immuno Allerg ,
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2022,
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2(2):1-9
- Instrument : NucleoCounter® YC-100™
- Cell Type: Yeast Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Two studies were performed to assess the efficacy of the treated yeast, Milmed, for the alleviation of physiologic health problems, mainly ‘common-cold’ and inflammation symptoms, performance improvements among racehorses in training. In Study I (n=8), Time to reduce pulse to...
View PublicationH3K9 dimethylation safeguards cancer cells against activation of the interferon pathway
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Hansen AM, Ge Y, Schuster MB, et al. ,
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Sci Adv ,
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2022,
-
8(11):eabf8627
- Instrument : NucleoCounter® NC-200™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Activation of interferon genes constitutes an important anticancer pathway able to restrict proliferation of cancer cells. Here, we demonstrate that the H3K9me3 histone methyltransferase (HMT) suppressor of variegation 3-9 homolog 1 (SUV39H1) is required for the proliferation of acute myeloid...
View PublicationEx Vivo Hydrostatic Pressure Loading of Atrial Tissues Activates Profibrotic Transcription via TGF-β Signal Pathway
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Zhang X, Yassouf Y, Huang K, et al. ,
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Int Heart J. ,
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2022,
-
63(2):367-374
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Excessive mechanical stress causes fibrosis-related atrial arrhythmia. Herein, we tried to investigate the mechanism of atrial fibrogenesis in response to mechanical stress by ex vivo approach. We collected atrial tissues from mice and then cultured them as “explants” under atmospheric...
View PublicationLongitudinal two-dimensional gas chromatography mass spectrometry as a non-destructive at-line monitoring tool during cell manufacturing identifies volatile features correlative to cell product quality
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Jimenez AC, Heist CA, Navaei M, et al. ,
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Cytotherapy ,
-
2022,
-
S1465-3249(22)00681-8
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background aims: Cell therapies have emerged as a potentially transformative therapeutic modality in many chronic and incurable diseases. However, inherent donor and patient variabilities, complex manufacturing processes, lack of well-defined critical quality attributes and unavailability of in-line or at-line process...
View PublicationEnhancing Adoptive Cell Therapy by T Cell Loading of SHP2 Inhibitor Nanocrystals before Infusion
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Li X, Halldórsdóttir HR, Weller S, et al. ,
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Nano ,
-
2022,
-
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Whereas adoptive T cell therapy has been extensively studied for cancer treatment, the response is still limited primarily due to immune dysfunction related to poor cell engraftment, tumor infiltration and engagement, and lack of a target. In addition, the modification...
View PublicationParenchymal and Inflammatory Cell Responses to Single and Repeated Ozone Exposure in Healthy and Surfactant Protein-C Mutant Lung
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Nguyen J, Deering-Rice CE, Armstrong BS, et al. ,
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Toxicol Sci ,
-
2022,
-
kfac074
- Instrument : NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Mutations in the alveolar epithelial-specific gene encoding for surfactant protein C (SP-C) are linked to pulmonary disease. Ozone (O3) is a ubiquitous pollutant known to exacerbate stress through oxidative injury and inflammation. To comprehend the structural, functional, and immunological impact...
View PublicationClinical and laboratory findings following transplantation of allogeneic adipose-derived mesenchymal stromal cells in knee osteoarthritis, a brief report
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Sadri B, Tamimi A, Nouraein S, et al. ,
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Connect Tissue Res ,
-
2022,
-
01/Dec
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
Background: Mesenchymal stromal cells (MSCs) injection has been proposed as an innovative treatment for knee osteoarthritis (KOA). Since, allogeneic MSCs can be available as off-the-shelf products, they are preferable in regenerative medicine. Among different sources for MSCs, adipose-derived MSCs (AD-MSCs)...
View PublicationEmulsion-templated microparticles with tunable stiffness and topology: Applications as edible microcarriers for cultured meat
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Norris SCP, Kawecki NS, Davis AR, et al. ,
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Biomaterials ,
-
2022,
-
287:121669
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cultured Meat
Cultured meat has potential to diversify methods for protein production, but innovations in production efficiency will be required to make cultured meat a feasible protein alternative. Microcarriers provide a strategy to culture sufficient volumes of adherent cells in a bioreactor...
View PublicationSingle-cell transcriptome and cell type-specific molecular pathways of human non-alcoholic steatohepatitis
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Fred RG, Steen Pedersen J, Thompson JJ, et al. ,
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Sci Rep ,
-
2022,
-
12(1):13484
- Instrument : NucleoCounter® NC-200™
- Cell Type: Hepatocytes
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The aim of this study is to characterize cell type-specific transcriptional signatures in non-alcoholic steatohepatitis (NASH) to improve our understanding of the disease. We performed single-cell RNA sequencing on liver biopsies from 10 patients with NASH. We applied weighted gene...
View PublicationUpscaling human mesenchymal stromal cell production in a novel vertical-wheel bioreactor enhances extracellular vesicle secretion and cargo profile
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Jeske R, Liu C, Duke L, et al. ,
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Bioactive Materials ,
-
2022,
-
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Human mesenchymal stromal cells (hMSCs) are mechanically sensitive undergoing phenotypic alterations when subjected to shear stress, cell aggregation, and substrate changes encountered in 3D dynamic bioreactor cultures. However, little is known about how bioreactor microenvironment affects the secretion and cargo...
View PublicationApplication of immune enhanced organoids in modeling personalized Merkel cell carcinoma research
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Forsythe SD, Erali RA, Laney P, et al. ,
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Sci Rep ,
-
2022,
-
12(1):13865.
- Instrument : NucleoCounter® NC-200™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Merkel cell carcinoma (MCC) is a rare neuroendocrine cutaneous cancer, with incidence of less than 1/100,000, low survival rates and variable response to chemotherapy or immunotherapy. Herein we explore the application of patient tumor organoids (PTOs) in modeling personalized research...
View PublicationAssessment of primary and inflammation-driven genotoxicity of carbon black nanoparticles in vitro and in vivo
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Di Ianni E, Møller P, Cholakova T, et al. ,
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Nanotoxicology ,
-
2022,
-
Jan/21
- Instrument : NucleoCounter® NC-200™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Carbon black nanoparticles (CBNPs) have a large surface area/volume ratio and are known to generate oxidative stress and inflammation that may result in genotoxicity and cancer. Here, we evaluated the primary and inflammatory response-driven (i.e. secondary) genotoxicity of two CBNPs,...
View PublicationImpact of SARS-CoV-2 RBD Mutations on the Production of a Recombinant RBD Fusion Protein in Mammalian Cells
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Gerez G, Martinez J, Steinbrugger C, et al. ,
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Biomolecules ,
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2022,
-
12(9):1170
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
SARS-CoV-2 receptor-binding domain (RBD) is a major target for the development of diagnostics, vaccines and therapeutics directed against COVID-19. Important efforts have been dedicated to the rapid and efficient production of recombinant RBD proteins for clinical and diagnostic applications. One...
View PublicationIn vitro Insect Fat Cultivation for Cellular Agriculture Applications
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Letcher SM, Rubio NR, Ashizawa RN, et al. ,
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ACS Biomater Sci Eng ,
-
2022,
-
- Instrument : NucleoCounter® NC-200™
- Cell Type: Insect Cells
- Assay: Cell Count & Viability Assay
- Research area: Cultured Meat
Cell-cultured fat could provide important elements of flavor, nutrition, and texture to enhance the quality and therefore expand consumer adoption of alternative meat products. In contrast to cells from livestock animals, insect cells have been proposed as a relatively low-cost...
View PublicationSurgical cotton microfibers loaded with nanoceria: A new platform for bone tissue engineering
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Singh S, Bhushan SB, Das A, et al. ,
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Ceramics International ,
-
2022,
-
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The number of bone damage/fractures has been increasing significantly over the world due to traumatic and accidental injuries. The process of bone regeneration is slowed by oxidative stress at the site of the bone defects. An increase in reactive oxygen...
View PublicationA lentiviral vector expressing a dendritic cell-targeting multimer induces mucosal anti-mycobacterial CD4+ T-cell immunity
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Anna F, Lopez J, Moncoq F, et al. ,
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Mucosal Immunol ,
-
2022,
-
10.1038/s41385-022-00566-z
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Virus Production
Most viral vectors, including the potently immunogenic lentiviral vectors (LVs), only poorly direct antigens to the MHC-II endosomal pathway and elicit CD4+ T cells. We developed a new generation of LVs encoding antigen-bearing monomers of collectins substituted at their C-terminal...
View PublicationDevelopment of process analytical tools for rapid monitoring of live virus vaccines in manufacturing
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Yi S, McCracken R, Davide J, et al. ,
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Sci Rep ,
-
2022,
-
12(1):15494
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Virus Production
In the development of end-to-end large-scale live virus vaccine (LVV) manufacturing, process analytical technology (PAT) tools enable timely monitoring of critical process parameters (CPP) and significantly guide process development and characterization. In a commercial setting, these very same tools can...
View PublicationEstablishment of In Vitro Models by Stress-Induced Premature Senescence for Characterizing the Stromal Vascular Niche in Human Adipose Tissue
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Wahlmueller M, Wahlmueller MS, Missfeldt K, et al. ,
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Life ,
-
2022,
-
12, 1459
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Acting as the largest energy reservoir in the body, adipose tissue is involved in longevity and progression of age-related metabolic dysfunction. Here, cellular senescence plays a central role in the generation of a pro-inflammatory environment and in the evolution of...
View PublicationDevelopment of an animal component free production process for Sabin inactivated polio vaccine
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Suarez-Zuluaga DA, van der Pol LA, van 't Oever AG, et al. ,
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Vaccine X ,
-
2022,
-
12:100223
- Instrument : NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Virus Production
Inactivated polio vaccine production using attenuated Sabin strains (sIPV) instead of wild type polio viruses (cIPV) is an initiative encouraged by the World Health Organization. This use of attenuated viruses is preferred as it reduces risks related to potential outbreaks...
View PublicationDevelopment of Robust Cell Therapy Manufacturing Processes Largely Depends on the Choice of Cell Counting Method Considering Cell Type, Precision, and Accuracy Requirements
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Dadgar S, Kuruvilla VM, Duvvuru H, et al. ,
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Int J Stem Cell Res Ther ,
-
2022,
-
9:074
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
Universal to all cell therapies (CT) is the need to determine the number of cells at each step of the manufacturing process as they are processed in different unit operations and developed into clinically relevant final CT products. Identifying a...
View PublicationRGD density along with substrate stiffness regulate hPSC hepatocyte functionality through YAP signalling
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Blackford SJI, Yu TTL, Norman MDA, et al. ,
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Biomaterials ,
-
2022,
-
121982
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Human pluripotent stem cell-derived hepatocytes (hPSC-Heps) may be suitable for treating liver diseases, but differentiation protocols often fail to yield adult-like cells. We hypothesised that replicating healthy liver niche biochemical and biophysical cues would produce hepatocytes with desired metabolic functionality....
View PublicationPPARβ/δ-Interfering Peptide Enhanced Mesenchymal Stromal Cell Immunoregulatory Properties
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Tejedor G, Boisguerin P, Vivès É, et al. ,
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Stem Cells Int ,
-
2022,
-
2022:5494749
- Instrument : NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
Background: Mesenchymal stem/stromal cells (MSCs) have been widely used for their therapeutic properties in many clinical applications including osteoarthritis. Despite promising preclinical results showing the ability of MSC to reduce the clinical severity of osteoarthritis (OA) in experimental animal models,...
View PublicationEfficient derivation and banking of clinical-grade human embryonic stem cell lines in accordance with Japanese regulations
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Takada K, Nakatani R, Moribe E, et al. ,
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Regen Ther ,
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2022,
-
21:553-559
- Instrument : NucleoCounter® NC-200™
- Cell Type: iPSCs & ESCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Introduction: We recently established clinical-grade human embryonic stem cell (hESC) line KthES11 in accordance with current good manufacturing practice standards in Japan. Despite this success, the establishment efficiency was very low at 7.1% in the first period. Methods: To establish...
View PublicationBispecific antibodies targeting CD40 and tumor-associated antigens promote cross-priming of T cells resulting in an antitumor response superior to monospecific antibodies
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Hägerbrand K, Varas L, Deronic A, et al. ,
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J Immunother Cancer ,
-
2022,
-
10(11):e005018
- Instrument : NucleoCounter® NC-200™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Background: Indications with poor T-cell infiltration or deficiencies in T-cell priming and associated unresponsiveness to established immunotherapies represent an unmet medical need in oncology. CD40-targeting therapies designed to enhance antigen presentation, generate new tumor-specific T cells, and activate tumor-infiltrating myeloid...
View PublicationMass cytometry analysis of blood from peanut-sensitized tolerant and clinically allergic infants
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Tursi AR, Saba NK, Dunham D, et al. ,
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Sci Data ,
-
2022,
-
9(1):738
- Instrument : NucleoCounter® NC-200™
- Cell Type: Whole Blood
- Assay: Cell Count & Viability Assay
- Research area: Cell-based immunotherapy
IgE-mediated food allergies in infants are a significant health concern, with peanut allergy being of particular interest due to its prevalence and severity. Among individuals who produce peanut-specific IgE some experience no adverse reaction on peanut consumption. This asymptomatic phenotype...
View PublicationEngineering Vascular Self-Assembly by Controlled 3D-Printed Cell Placement
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Orellano I, Thomas A, Herrera A, et al. ,
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Advanced Functional Materials ,
-
2022,
-
32(52):2208325
- Instrument : NucleoCounter® NC-200™
- Cell Type: Primary Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Nutrient supply via a functional vasculature is essential during regenerative processes, tissue growth, and homeostasis. 3D bioprinting offers the opportunity to engineer vascularized constructs by combining cells and biocompatible materials in specifically designed fashions. However, the complexity of microvascular dynamic...
View PublicationMonocyte-Platelet Aggregates Triggered by CD31 Molecule in Non-ST Elevation Myocardial Infarction: Clinical Implications in Plaque Rupture
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Vinci R, Pedicino D, Bonanni A, et al. ,
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Front Cardiovasc Med ,
-
2022,
-
8:741221
- Instrument : NucleoCounter® NC-200™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Despite the recent innovations in cardiovascular care, atherothrombosis is still a major complication of acute coronary syndromes (ACS). We evaluated the involvement of the CD31 molecule in thrombotic risk through the formation of monocyte-platelet (Mo-Plt) aggregates in patients with ACS...
View PublicationImproving cell viability using counterflow centrifugal elutriation
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Li A, Barabadi M, McDonald H, et al. ,
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Cytotherapy ,
-
2022,
-
24(6):650-658
- Instrument : NucleoCounter® NC-202™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Cell viability is an important release criterion in the manufacturing of cell therapy products. Low cell viability can have significant impact on product quality and manufacturing efficiency. Counterflow centrifugation technology has been applied to the manufacturing of cell therapy...
View PublicationMapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq
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Replogle JM, Saunders RA, Pogson AN, et al. ,
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Cell ,
-
2022,
-
185(14):2559-2575.e28
- Instrument : NucleoCounter® NC-202™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
A central goal of genetics is to define the relationships between genotypes and phenotypes. High-content phenotypic screens such as Perturb-seq (CRISPR-based screens with single-cell RNA-sequencing readouts) enable massively parallel functional genomic mapping but, to date, have been used at limited...
View PublicationScaffold-mediated switching of lymphoma metabolism in culture
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Bhatt R, Ravi D, Evens AM, Parekkadan B ,
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Cancer Metab ,
-
2022,
-
10(1):15
- Instrument : NucleoCounter® NC-202™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Background: Diffuse large B cell lymphoma (DLBCL) is an aggressive subtype of non-Hodgkin lymphoma (NHL) and accounts for about a third of all NHL cases. A significant proportion (~40%) of treated DLBCL patients develop refractory or relapsed disease due to...
View PublicationCircular single-stranded DNA is a superior homology-directed repair donor template for efficient genome engineering
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Xie K, Starzyk J, Majumdar I, et al. ,
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bioRxiv ,
-
2022,
-