Reagent B

Stabilizing Buffer

Product number: 910-0002
Size: 500 ml

Storage: Room temperature

Reagent B is a weak alkaline aqueous solution of surfactant and inorganic acid. The pH value is in the range of 8.00-9.00. Reagent B is used for neutralization of weak acidic solutions which is of particular importance for DNA quantification. It is used in conjunction with Reagent A100 to raise the sample’s pH value and avoid DNA degradation as well as improve the binding efficiency of DAPI.

Reagent B facilitates precise DNA content quantification of acidic cell samples by raising the pH. Neutralization of acidic cell samples is important for DNA stabilization, and furthermore potentiates the fluorescence signals of various dyes, such as Propidium Iodide (PI). Please refer to the specific Application Notes for detailed instructions.

The solution is for research use only (RUO) and is not for diagnostic or therapeutic use. Please refer to the documents below for further details. Order the product in our Webshop.

Use for

Total cell count with the NucleoCounter® NC-100™.
Count of Aggregated Cells A100 and B Assay with the NucleoCounter® NC-200™, NC-250™ and NC-3000™.

Newsletter
Contact us