Cell Count & Viability Assay
Detection of Total and Dead Cell Populations Using Fluorescent Cytometry
- Fast and convenient cell count and viability measurement
- Consistency across instruments, users and sites
- Objective analysis – no human error in sample preparation
- Scalable system with 21 CFR Part 11 / GMP-ready software
- Personal support by our dedicated experts
- Specialized protocols for mammalian and insect cells
- Automated PDF reports
- Export data in FCS/ACS formats (for NC-3000™)
Overview of the Cell Count & Viability Assay
With the NucleoCounter® family of cell counters, you can count your cells quickly and easily. Our cell counting assays are accurate, precise, and versatile, meeting the needs of even the most specialized cell types and cultures.
We offer several instruments with cell counting assays that are optimized for different applications:
- NucleoCounter® NC-200™/ NucleoCounter® NC-202™ for straightforward cell counting in all cell cultures
- NucleoCounter® NC-3000™ combines cell counting with advanced image cytometry
- NucleoCounter® SP-100™ for sperm cells
- NucleoCounter® YC-100™ for yeast cells
- NucleoCounter® SCC-100™ for somatic cells
How Our Cell Count & Viability Assay Works
Our NucleoCounter® instruments use automated fluorescence cytometry to count cells and determine viability. They rely on fluorescent dyes, acridine orange (AO) and 4′,6-diamidino-2-phenylindole (DAPI), to identify live and dead cells and provide accurate cell counts.
AO is a membrane-permeable dye that binds to nucleic acids, staining all the cells in a sample. DAPI cannot penetrate the cell membrane, hence it only stains non-viable cells with a damaged cell membrane. Detecting the signals from these two fluorescent dyes allows us to exclude debris and count cells accurately and consistently, with no subjectivity about what is or is not counted as a cell.
Trusted by Experts
Thanks to their versatility, our cell counting instruments are used by thousands of customers around the globe in a vast range of applications, including cell and gene therapy, therapeutic protein production, vaccine manufacturing, bioprocessing, clean meat, animal reproduction, and research. Some recently published examples include:
- Researchers at the Jenner Institute used a NucleoCounter® to monitor the cell density of their HEK cells during their adenovirus-vectored vaccine manufacturing processes. Their work was published recently on bioRxiv.
- Research from Theresienkrankenhaus showed that in patients with osteoarthritis of the knee who received adipogenic stromal-vascular fraction therapy, a greater number of cells (measured with a NucleoCounter®) correlated with better outcomes for patients.
- The Hackensack University Medical Center used a NucleoCounter® to assess viability of fresh and cryopreserved hematopoietic progenitor cell products before batch release.
- Scientists at the Autonomous University of Barcelona used a NucleoCounter® to monitor the production of virus-like particles.
You can read more examples of our cell counting assays in action on our customer projects page.
The Advantages of NucleoCounter® Cell Counting
Precise, Objective Analysis
Precise, repeatable results mean confidence in your processes, research, and decisions. Our cell counting assays use a comprehensive approach to eliminate human error and guarantee precise, objective cell counting:
- We use fluorescence microscopy and image analysis to quantify cells automatically. NucleoCounter® instruments used fixed focus and exposure, so no adjustments are required, removing the potential for human error or inter-user variation.
- All our instruments are calibrated against a reference instrument, ensuring that every machine gives the same result, no matter where they are or who is operating them.
- Our cassettes are individually volume calibrated to ensure high precision. Furthermore, they contain immobilized cell counting dyes, making staining a closed, standardized process that eliminates pipetting errors.
- Our software performs a series of analysis checks to ensure optimal sample analysis and will tell you if there are too few or too many cells in the sample to ensure accurate results.
Our instruments, cassettes, and software combine to provide exact cell counting and low coefficients of variation, no matter who does the analysis, where they do it, or which instrument they use. As a result, our NucleoCounter® instruments provide lower variation than other cell counting assays. For example, the NC-202™ typically provides a coefficient of variation below 5%.
Fast & Easy to Use
The user-friendly nature of the NucleoCounter® instruments allows anyone to measure cell viability. There are three simple steps:
- Put the cassette into your sample and press the piston
- Load the cassette into your NucleoCounter®
- Measure the cell count and viability of your sample using our universal cell counting protocol
The results are available within 1 minute. Our accompanying software displays your results and allows you to see the viable and non-viable cells with the click of a button. Your data can be exported to different formats and extracted into PDF reports.
GMP Compliance Made Easy
Our protocols eliminate variability and subjectivity from the cell counting process, ensuring that your results are precise and consistent, no matter who is conducting the count. What’s more, our simple workflows are ideal for short, easy to understand SOPs. Consequently, validating your cell counting methods for GMP compliance is straightforward and pain-free. Our NucleoCounter® NC-200™, NC-202™, NC-250™, and NC-3000™ instruments are also Annex-11 and 21 CFR Part 11-ready.
Count All Cell Types
Our cell counting assays are universal and can count all nucleated cells. As a result, you can use our NucleoCounter® instruments with even the most specialized cell types and culture conditions, including:
Primary mammalian cells | Natural killer (NK) cells |
Leukocytes | Insect cells |
Chinese hamster ovary (CHO) cells | Yeast |
Mesenchymal stem cells (MSCs) | Whole blood (WBC) |
Induced pluripotent stem cells (iPSCs) | Stromal vascular fractions |
Peripheral blood mononuclear cells (PBMCs) | Microcarriers |
Hepatocytes | Spheroids |
T cells | Aggregated cells |
Quick On-Demand Support
Our NucleoCounter® instruments all come with access to our scientists and cell counting experts. Our world-class team will support you throughout product installation and protocol development. We also offer training, application support, and dedicated field service teams to ensure you achieve optimal instrument performance and the best quality results.
To learn more about our cell counting solutions, visit our products page or contact us.
Tech notes
Publications
SARS-CoV2 wild type and mutant specific humoral and T cell immunity is superior after vaccination than after natural infection
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Richardson JR, Götz R, Mayr V, et al. ,
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PLoS One ,
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2022,
-
17(4):e0266701
- Instrument: NucleoCounter® NC-250™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Objective: We investigated blood samples from fully SARS-CoV2-vaccinated subjects and from previously positive tested patients up to one year after infection with SARS-CoV2, and compared short- and long-term T cell and antibody responses, with a special focus on the recently...
View PublicationDisruption of Membrane Integrity as a Molecular Initiating Event Determines the Toxicity of Polyhexamethylene Guanidine Phosphate Depending on the Routes of Exposure
-
Song J, Jung KJ, Yang MJ, et al. ,
-
Int J Mol Sci ,
-
2022,
-
23(6):3289
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Polyhexamethylene guanidine phosphate (PHMG-P), a cationic biocide, is widely used in household products due to its strong bactericidal activity and low toxicity. However, it causes fatal lung damage when inhaled. In this study, we investigated why PHMG-P causes fatal lung...
View PublicationThe effects of air pollutants exposure on the transmission and severity of invasive infection caused by an opportunistic pathogen Streptococcus pyogenes
-
Zhi Y, Chen X, Cao G, et al. ,
-
Environ Pollut ,
-
2022,
-
119826
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Currently, urbanization is associated with an increase in air pollutants that contribute to invasive pathogen infections by altering the host’s innate immunity and antimicrobial resistance capability. Streptococcus pyogenes, also known as Group A Streptococcus (GAS), is a gram-positive opportunistic pathogen...
View PublicationPolyhexamethylene guanidine phosphate-induced necrosis may be linked to pulmonary fibrosis
-
Kang MS, Kim SH, Yang MJ, et al. ,
-
Toxicol Lett ,
-
2022,
-
362:1-16
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Following the humidifier disinfectant incident in Korea, polyhexamethylene guanidine phosphate (PHMG-P) has been used to establish lung fibrosis model animals. Herein, we investigated time-dependent changes after a single PHMG-P instillation (22 μg/lung) to identify the underlying pathogenesis and immune response...
View PublicationComprehensive Targeted Metabolomic Study in the Lung, Plasma, and Urine of PPE/LPS-Induced COPD Mice Model
-
Kim HY, Lee HS, Kim IH, et al. ,
-
Int J Mol Sci ,
-
2022,
-
23(5):2748
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
(1) Background: Progression of chronic obstructive pulmonary disease (COPD) leads to irreversible lung damage and inflammatory responses; however, biomarker discovery for monitoring of COPD progression remains challenging. (2) Methods: This study evaluated the metabolic mechanisms and potential biomarkers of COPD...
View PublicationSublethal pulmonary toxicity screening of silica nanoparticles in rats after direct intratracheal instillation
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Han HY ,
-
Toxicological Research ,
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2022,
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The present aimed to characterize the toxicity of silica nanoparticles in Sprague Dawley rats and determine the dose levels for a repeated-dose toxicity study. Silica nanoparticles (SiO2, 20 nm and 50 nm) were administered as a single intratracheal instillation of...
View PublicationRapid Antibody Glycoengineering in CHO Cells Via RNA Interference and CGE-LIF N-Glycomics
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Kotidis P, Marbiah M, Donini R, et al. ,
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Methods Mol Biol ,
-
2022,
-
2370:147-167
- Instrument: NucleoCounter® NC-250™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Graft-contaminating tumor cells correlate with inferior outcome in high-risk neuroblastoma patients undergoing hematopoietic stem cell transplantation and can contribute to relapse. Motivated by the potential therapeutic benefit of tumor cell removal as well as the high prognostic and diagnostic...
View PublicationCharacterization of the Antibacterial Activity of an SiO 2 Nanoparticular Coating to Prevent Bacterial Contamination in Blood Products
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Fonseca S, Cayer MP, Ahmmed KMT, et al. ,
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Antibiotics ,
-
2022,
-
11(1):107
- Instrument: NucleoCounter® NC-250™
- Cell Type: Whole Blood
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Technological innovations and quality control processes within blood supply organizations have significantly improved blood safety for both donors and recipients. Nevertheless, the risk of transfusion-transmitted infection remains non-negligible. Applying a nanoparticular, antibacterial coating at the surface of medical devices is...
View PublicationSoft-sensors application for automated feeding control in high-throughput mammalian cell cultures
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Martínez-Monge I, Martínez C, Decker M, et al. ,
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Biotechnol Bioeng ,
-
2022,
- Instrument: NucleoCounter® NC-250™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The ever-increasing demand for biopharmaceuticals has created the need for improving the overall productivity of culture processes. One such operational concept that is considered is fed-batch operations as opposed to batch operations. However, optimal fed-batch operations require complete knowledge of...
View PublicationMolecular characteristics and spatial distribution of adult human corneal cell subtypes
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Ligocki AJ, Fury W, Gutierrez C, et al. ,
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Sci Rep ,
-
2021,
-
11(1):16323
- Instrument: NucleoCounter® NC-250™
- Cell Type: Dendritic Cells
- Assay: Cell Count & Viability Assay
- Research area: Ophthalmology
Bulk RNA sequencing of a tissue captures the gene expression profile from all cell types combined. Single-cell RNA sequencing identifies discrete cell-signatures based on transcriptomic identities. Six adult human corneas were processed for single-cell RNAseq and 16 cell clusters were...
View PublicationAnti-obesity activity of Heracleum moellendorffii root extracts in 3T3-L1 adipocytes
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Geum NG, Son HJ, Yeo JH, et al. ,
-
Food Sci Nutr ,
-
2021,
-
9(11):5939-5945
- Instrument: NucleoCounter® NC-250™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
It has been reported that H. mollendorffii roots (HMR) have various pharmacological activities such as anti-inflammatory activity and immunostimulatory activity. However, the anti-obesity activity of HMR has not been studied. Thus, we evaluated in vitro anti-obesity of HMR in mouse...
View PublicationLabel-free separation of neuroblastoma patient-derived xenograft (PDX) cells from hematopoietic progenitor cell products by acoustophoresis
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Olm F, Panse L, Dykes JH, et al. ,
-
Stem Cell Res Ther ,
-
2021,
-
12(1):542
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
The impact of the glycan distribution on the in vivo function and half-life of monoclonal antibodies has long motivated the genetic engineering of producer cells to achieve structures that enhance efficacy, safety and stability. To facilitate glycoengineering of IgG-producing Chinese...
View PublicationQuantitative evaluation of chromosomal rearrangements in gene-edited human stem cells by CAST-Seq
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Turchiano G, Andrieux G, Klermund J, et al. ,
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Cell Stem Cell ,
-
2021,
-
28(6):1136-1147.e5
- Instrument: NucleoCounter® NC-250™
- Cell Type: HSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The craniofacial disorder mandibulofacial dysostosis Guion-Almeida type is caused by haploinsufficiency of the U5 snRNP gene EFTUD2/SNU114. However, it is unclear how reduced expression of this core pre-mRNA splicing factor leads to craniofacial defects. Here we use a CRISPR-Cas9 nickase...
View PublicationIntegrating Human-Induced Pluripotent Stem Cell Expansion Capability and Cardiomyocyte Differentiation Potential in a Microcarrier Suspension Culture
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Ho V, Tong G, Lam A, et al. ,
-
Methods Mol Biol ,
-
2021,
- Instrument: NucleoCounter® NC-250™
- Assay: Cell Count & Viability Assay
- Research area: Cardiology
Human-induced pluripotent stem cells are known for their high proliferation capacity as well as their ability to differentiate to different lineages (Ban et al., Theranostics 7(7):2067–2077, 2017; Chen et al., Stem Cell Res 15(2):365–375, 2015; Serra et al., Trends Biotechnol...
View PublicationThe histone methyltransferase Ezh2 restrains macrophage inflammatory responses
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Kitchen GB, Hopwood T, Gali Ramamoorthy T, et al. ,
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FASEB J ,
-
2021,
-
35(10):e21843
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Robust inflammatory responses are critical to survival following respiratory infection, with current attention focused on the clinical consequences of the Coronavirus pandemic. Epigenetic factors are increasingly recognized as important determinants of immune responses, and EZH2 is a prominent target due...
View PublicationSoluble PD-1 (sPD-1) is expressed in human macrophages
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Antonsen KW, Hviid CVB, Hagensen MK, et al. ,
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Cell Immunol ,
-
2021,
-
369:104435
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
The PD-1/PD-L1 axis plays a crucial role in regulating the anti-tumour immune response. A soluble PD-1 protein (sPD-1) has previously been observed, which could block the binding of PD-L1 to PD-1. Tumour associated macrophages are abundant in tumours, and evidence...
View PublicationPreexisting and post-COVID-19 immune responses to SARS-CoV-2 in cancer patients
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Bilich T, Roerden M, Maringer Y, et al. ,
-
Cancer Discov ,
-
2021,
-
candisc.0191.2021
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Background: Metformin, a biguanide, is one of the most commonly prescribed treatments for type 2 diabetes and has recently been recommended as a potential drug candidate for advanced cancer therapy. Although Metformin has antiproliferative and proapoptotic effects on breast cancer,...
View PublicationRepeated intratracheal instillation of zinc oxide nanoparticles induced pulmonary damage and a systemic inflammatory response in cynomolgus monkeys
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Park EJ, Kim SN, Yoon C, et al. ,
-
Nanotoxicology ,
-
2021,
-
Jan/15
- Instrument: NucleoCounter® NC-250™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Pancreatic islets comprise of endocrine cells with distinctive hormone expression patterns. The endocrine cells show functional differences in response to normal and pathological conditions. The goal of this protocol is to generate high-quality, large-scale transcriptome data of each endocrine cell...
View PublicationPreclinical Evaluation of a Novel TALEN Targeting CCR5 Confirms Efficacy and Safety in Conferring Resistance to HIV-1 Infection
-
Romito M, Juillerat A, Kok YL, et al. ,
-
Biotechnol J ,
-
2021,
-
16(1):e2000023
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Background: The thiosemicarbazone CD 02750 (VLX50) was recently reported as a hit compound in a phenotype-based drug screen in primary cultures of patient tumor cells. We synthesized a copper complex of VLX50, denoted VLX60, and characterized its antitumor and mechanistic...
View PublicationAcoustophoresis Enables the Label-Free Separation of Functionally Different Subsets of Cultured Bone Marrow Stromal Cells
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Olm F, Lim HC, Schallmoser K, et al. ,
-
Cytometry A ,
-
2021,
-
99(5):476-487
- Instrument: NucleoCounter® NC-250™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay, Cell Cycle Assay
- Research area: Stem Cell Therapy
Objective: As the development and progression of colorectal cancer (CRC) are known to be affected by the immune system, cell subsets such as T cells, natural killer (NK) cells, and natural killer T (NKT) cells are considered interesting targets for...
View PublicationTargeting EML4-ALK gene fusion variant 3 in thyroid cancer
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Aydemirli MD, van Eendenburg JDH, van Wezel T, et al. ,
-
Endocr Relat Cance ,
-
2021,
-
28(6):377-389
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
The WD40-containing E3 ubiquitin ligase RFWD3 has been recently linked to the repair of DNA damage by homologous recombination (HR). Here we have shown that an RFWD3 mutation within the WD40 domain is connected to the genetic disease Fanconi anemia...
View PublicationInhalation exposure by cigarette smoke: Effects on the progression of bleomycin- and lipopolysaccharide-induced lung injuries in rat models
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Lee SM, Shin YJ, Go RE, et al. ,
-
Toxicology ,
-
2021,
-
451:152695
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Currently available toxicity data on humidifier disinfectants are primarily limited to polyhexamethylene guanidine phosphate-induced lung fibrosis. We, therefore, investigated whether the sterilizer component Kathon, which is a mixture of chloromethylisothiazolinone and methylisothiazolinone, induces fibrotic lung injury following direct lung exposure...
View PublicationDiesel Exhaust Particulates Enhances Susceptibility of LPS-Induced Acute Lung Injury through Upregulation of the IL-17 Cytokine-Derived TGF-β1/Collagen I Expression and Activation of NLRP3 Inflammasome Signaling in Mice
-
Kim DI, Song MK, Lee K ,
-
Biomolecules ,
-
2021,
-
11(1):67
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
The potential of adoptive cell therapy can be extended when combined with genome editing. However, variation in the quality of the starting material and the different manufacturing steps are associated with production failure and product contamination. Here, we present an...
View PublicationOsmolality Effects on CHO Cell Growth, Cell Volume, Antibody Productivity and Glycosylation
-
Alhuthali S, Kotidis P, Kontoravdi C ,
-
International Journal of Molecular Sciences ,
-
2021,
-
22(7):3290
- Instrument: NucleoCounter® NC-250™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
MCF7 cells acquire estrogen-independent proliferation after long-term estrogen deprivation (LTED), which recapitulates endocrine therapy resistance. LTED cells can become primed for apoptosis, but the underlying mechanism is largely unknown. We previously reported that Eleanor non-coding RNAs (ncRNAs) upregulate the ESR1...
View PublicationKathon Induces Fibrotic Inflammation in Lungs: The First Animal Study Revealing a Causal Relationship between Humidifier Disinfectant Exposure and Eosinophil and Th2-Mediated Fibrosis Induction
-
Song MK, Kim DI, Lee K ,
-
Molecules ,
-
2020,
-
25(20):4684
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The ever-growing biopharmaceutical industry relies on the production of recombinant therapeutic proteins in Chinese hamster ovary (CHO) cells. The traditional timelines of CHO cell line development can be significantly shortened by the use of targeted gene integration (TI). However, broad...
View PublicationCausal relationship between humidifier disinfectant exposure and Th17-mediated airway inflammation and hyperresponsiveness
-
Song MK, Kim DI, Lee K ,
-
Toxicology ,
-
2021,
-
454:152739
- Instrument: NucleoCounter® NC-250™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Diesel exhaust particulates (DEP) have adverse effects on the respiratory system. Endoplasmic reticulum (ER) abnormalities contribute to lung inflammation. However, the relationship between DEP exposure and ER stress in the respiratory immune system and especially the alveolar macrophages (AM) is...
View PublicationAn Exploratory Study of the Relationships Between Diesel Engine Exhaust Particle Inhalation, Pulmonary Inflammation and Anxious Behavior
-
Jeong S, Lee JH, Ha JH, et al. ,
-
International Journal of Environmental Research and Public Health ,
-
2021,
-
18(3):1166
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Genome editing has shown great promise for clinical translation but also revealed the risk of genotoxicity caused by off-target effects of programmable nucleases. Here we describe chromosomal aberrations analysis by single targeted linker-mediated PCR sequencing (CAST-Seq), a preclinical assay to...
View PublicationAutomated generation of gene-edited CAR T cells at clinical scale
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Alzubi J, Lock D, Rhiel M, et al. ,
-
Mol Ther Methods Clin Dev ,
-
2020,
-
20:379-388
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Therapies to treat patients infected with human immunodeficiency virus (HIV) aim at preventing viral replication but fail to eliminate the virus. Although transplantation of allogeneic CCR5Δ32 homozygous stem cell grafts provided a cure for a few patients, this approach is...
View PublicationCD163 as a Biomarker in Colorectal Cancer: The Expression on Circulating Monocytes and Tumor-Associated Macrophages, and the Soluble Form in the Blood
-
Krijgsman D, De Vries NL, Andersen MN, et al. ,
-
Int J Mol Sci ,
-
2020,
-
21(16):5925
- Instrument: NucleoCounter® NC-250™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Oncology
The conventional outflow pathway is a complex tissue responsible for maintaining intraocular pressure (IOP) homeostasis. The coordinated effort of multiple cells with differing responsibilities ensures healthy outflow function and IOP maintenance. Dysfunction of one or more resident cell types results...
View PublicationMolecular taxonomy of human ocular outflow tissues defined by single-cell transcriptomics
-
Patel G, Fury W, Yang H, et al. ,
-
Proc Natl Acad Sci U S A ,
-
2020,
-
117(23):12856-12867
- Instrument: NucleoCounter® NC-250™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Ophthalmology
Recently, some researchers have demonstrated that inhaled zinc oxide nanoparticles (ZnONPs) induce an acute systemic inflammatory response in workers. Considering nonhuman primates are preferably considered an animal model for translational research due to their proven similarity with humans in terms...
View PublicationMulticopy Targeted Integration for Accelerated Development of High-Producing Chinese Hamster Ovary Cells
-
Sergeeva D, Lee GM, Nielsen LK, Grav LM ,
-
ACS Synth Biol ,
-
2020,
-
9(9):2546-2561
- Instrument: NucleoCounter® NC-250™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
The formation and function of highly specialized cells and tissues in a multicellular organism from a single genome are enabled through differential spatiotemporal access to the information contained in the genomic DNA. The epigenome plays an essential role in how...
View PublicationDiesel Exhaust Particulates Induce Neutrophilic Lung Inflammation by Modulating Endoplasmic Reticulum Stress-Mediated CXCL1/KC Expression in Alveolar Macrophages
-
Kim DI, Song MK, Kim HI, et al. ,
-
Molecules ,
-
2020,
-
25(24):6046
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Chondrosarcomas are chemo- and radiotherapy resistant and frequently harbor mutations in isocitrate dehydrogenase (IDH1 or IDH2), causing increased levels of D-2-hydroxyglutarate (D-2-HG). DNA repair defects and synthetic lethality with poly(ADP-ribose) polymerase (PARP) inhibition occur in IDH mutant glioma and leukemia...
View PublicationModeling MyD88 Deficiency In Vitro Provides New Insights in Its Function
-
Craig-Mueller N, Hammad R, Elling R, et al. ,
-
Front Immunol ,
-
2020,
-
11:608802
- Instrument: NucleoCounter® NC-250™
- Assay: Cell Count & Viability Assay
- Research area: Gene Therapy
Emergence and dissemination of multidrug resistance among pathogenic Escherichia coli have posed a serious threat to public health across developing and developed countries. In combination with a flexible repertoire of virulence mechanisms, E. coli can cause a vast range of...
View PublicationA versatile reporter system for multiplexed screening of effective epigenome editors
-
Roman Azcona MS, Fang Y, Carusillo A, et al. ,
-
Nat Protoc ,
-
2020,
-
15(10):3410-3440
- Instrument: NucleoCounter® NC-250™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Cancer patients, in particular patients with hematological malignancies, are at increased risk for critical illness upon COVID-19. We here assessed antibody as well as CD4+ and CD8+ T cell responses in unexposed and SARS-CoV-2-infected cancer patients to characterize SARS-CoV 2...
View PublicationRegulatory T cells confer a circadian signature on inflammatory arthritis
-
Hand LE, Gray KJ, Dickson SH, et al. ,
-
Nature Communications ,
-
2020,
-
11(1):1658
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Background: Fanconi anemia (FA) is an inherited genomic instability disorder with congenital and developmental abnormalities, bone marrow failure and predisposition to cancer early in life, and cellular sensitivity to DNA interstrand crosslinks. Case presentation: A fifty-one-year old female patient, initially...
View PublicationParticle movement and fluid behavior visualization using an optically transparent 3D-printed micro-hydrocyclone
-
Syed MS, Mirakhorli F, Marquis C, et al. ,
-
Biomicrofluidics ,
-
2020,
-
14(6):064106
- Instrument: NucleoCounter® NC-250™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
HCF-2 is a member of the host-cell-factor protein family, which arose in early vertebrate evolution as a result of gene duplication. Whereas its paralog, HCF-1, is known to act as a versatile chromatin-associated protein required for cell proliferation and differentiation,...
View PublicationSingle-cell RNA Sequencing and Analysis of Human Pancreatic Islets
-
Xin Y, Adler C, Kim J, et al. ,
-
J Vis Exp ,
-
2019,
-
-149
- Instrument: NucleoCounter® NC-250™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The toxic substances of cigarette smoke (CS) induce inflammatory responses in the lung by recruiting inflammatory cells. In this study, we investigated the effects of CS on the progression of lung disease in bleomycin (BLM) and lipopolysaccharide (LPS)-induced lung injury...
View PublicationDisease modeling of core pre-mRNA splicing factor haploinsufficiency
-
Wood KA, Rowlands CF, Qureshi WMS, et al. ,
-
Hum Mol Genet ,
-
2019,
-
28(22):3704-3723
- Instrument: NucleoCounter® NC-250™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: The circadian clock plays a crucial role in regulating physiology and is important for maintaining immune homeostasis and responses to inflammatory stimuli. Inflammatory arthritis often shows diurnal variation in disease symptoms and disease markers, and it is now established...
View PublicationMixed mucosal-parenteral immunizations with the broadly conserved pathogenic Escherichia coli antigen SslE induce a robust mucosal and systemic immunity without affecting the murine intestinal microbiota
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Naili I, Vinot J, Baudner BC, et al. ,
-
Vaccine ,
-
2019,
-
37(2):314-324
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Inherited defects in MyD88 and IRAK4, two regulators in Toll-like receptor (TLR) signaling, are clinically highly relevant, but still incompletely understood. MyD88- and IRAK4-deficient patients are exceedingly susceptible to a narrow spectrum of pathogens, with ∼50% lethality in the first...
View PublicationCharacterization of circulating T-, NK-, and NKT cell subsets in patients with colorectal cancer: the peripheral blood immune cell profile
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Krijgsman D, de Vries NL, Skovbo A, et al. ,
-
Cancer Immunol Immunother ,
-
2019,
-
68(6):1011-1024
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Recently, particle concentration and filtration using inertial microfluidics have drawn attention as an alternative to membrane and centrifugal technologies for industrial applications, where the target particle size varies between 1 µm and 500 µm. Inevitably, the bigger particle size (>50...
View PublicationThe circadian regulator Bmal1 in joint mesenchymal cells regulates both joint development and inflammatory arthritis
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Hand LE, Dickson SH, Freemont AJ, et al. ,
-
Arthritis Res Ther ,
-
2019,
-
21(1):5
- Instrument: NucleoCounter® NC-250™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Mesenchymal stromal cells (MSCs) have shown promising results for the treatment of refractory acute graft-versus-host disease. While safety of MSC infusion has been demonstrated, the use of cryopreserved MSCs in clinical trials has raised concerns regarding the retention of...
View PublicationHCF-2 inhibits cell proliferation and activates differentiation-gene expression programs
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Gudkova D, Dergai O, Praz V, Herr W ,
-
Nucleic Acids Res ,
-
2019,
-
47(11):5792-5808
- Instrument: NucleoCounter® NC-250™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
HCF-2 is a member of the host-cell-factor protein family, which arose in early vertebrate evolution as a result of gene duplication. Whereas its paralog, HCF-1, is known to act as a versatile chromatin-associated protein required for cell proliferation and differentiation,...
View PublicationCircadian variation in pulmonary inflammatory responses is independent of rhythmic glucocorticoid signaling in airway epithelial cells
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Ince LM, Zhang Z, Beesley S, et al. ,
-
FASEB J ,
-
2019,
-
33(1):126-139
- Instrument: NucleoCounter® NC-250™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Monoclonal antibodies are crucial in the treatment of a broad spectrum of critical illnesses such as cancer and autoimmune diseases. These biopharmaceuticals are industrially produced in fed-batch animal cell culture bioreactors to achieve high cell density and product titre. At...
View PublicationTargeted Treatment Options of Recurrent Radioactive Iodine Refractory Hürthle Cell Cancer
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Aydemirli MD, Corver W, Beuk R, et al. ,
-
Cancers ,
-
2019,
-
11(8):1185
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Inhalation of diesel exhaust particulate (DEP) causes oxidative stress-induced lung inflammation. This study investigated the protective effects of TF-343, an antioxidant and anti-inflammatory agent, in mouse and cellular models of DEP-induced lung inflammation as well as the underlying molecular mechanisms....
View PublicationScaled-Up Inertial Microfluidics: Retention System for Microcarrier-Based Suspension Cultures
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Moloudi R, Oh S, Yang C, et al. ,
-
Biotechnol J ,
-
2019,
-
14(5):e1800674
- Instrument: NucleoCounter® NC-250™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Immunosenescence is believed to be responsible for poor vaccine efficacy in the elderly. To overcome this difficulty, research into vaccination strategies and the mechanisms of immune responses to vaccination is required. By analyzing the innate and adaptive immune responses to...
View PublicationLabel-free neuroblastoma cell separation from hematopoietic progenitor cell products using acoustophoresis – towards cell processing of complex biological samples
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Olm F, Urbansky A, Dykes JH, et al. ,
-
Sci Rep ,
-
2019,
-
9(1):8777
- Instrument: NucleoCounter® NC-250™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
2,3,5,4′-Tetrahydroxystilbene-2-O-β-d-glucoside (THSG) is an active compound extracted from Polygonum multiflorum Thunb. This herb and radix Polygoni Multiflori preparata have been used to treat arteriosclerosis, hyperlipidemia, hypercholesterolemia, and diabetes for thousands of years. This study aimed to investigate the protective effects...
View PublicationEnhanced Efficacy of Vaccination With Vaccinia Virus in Old vs. Young Mice
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Shmeleva EV, Smith GL, Ferguson BJ ,
-
Front Immunol ,
-
2019,
-
1,652777778
- Instrument: NucleoCounter® NC-250™
- Cell Type: Whole Blood
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: The respiratory system is exposed to various allergens via inhaled and intranasal routes. Murine models of allergic lung disease have been developed to clarify the mechanisms underlying inflammatory responses and evaluate the efficacy of novel therapeutics. However, there have...
View PublicationGenome-wide effect of pulmonary airway epithelial cell–specific Bmal1 deletion
-
Zhang Z, Hunter L, Wu G, et al. ,
-
FASEB J ,
-
2019,
-
33(5):6226-6238
- Instrument: NucleoCounter® NC-250™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Purpose: We wanted to investigate effects of vitamin D3 (25(OH)D3 and 1.25(OH)2D3) on inflammatory cytokine expression in both activated and non-activated Mφ. Materials and methods: Mononuclear cells, isolated from healthy donor buffy coats were cultured for a 6-day differentiation-period. Fully...
View PublicationTF-343 Alleviates Diesel Exhaust Particulate-Induced Lung Inflammation via Modulation of Nuclear Factor-κB Signaling
-
Kim DI, Song MK, Kim SH, et al. ,
-
J Immunol Res ,
-
2019,
-
2019:8315845
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The manufacture of protein-based therapeutics presents unique challenges due to limited control over the biotic phase. This typically gives rise to a wide range of protein structures of varying safety and in vivo efficacy. Herein we propose a computational methodology,...
View PublicationConstrained global sensitivity analysis for bioprocess design space identification
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Kotidis P, Demis P, Goey CH, et al. ,
-
Computers and Chemical Engineering ,
-
2019,
-
125:558–568
- Instrument: NucleoCounter® NC-250™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The immunomodulatory drug thalidomide, and its analogs, lenalidomide, and pomalidomide (IMiDs), have become essential components of the standard treatment for multiple myeloma (MM), and have led to significant improvement of survival in patients with this devastating disease. Cereblon (CRBN), the...
View PublicationComparison of asthma phenotypes in OVA-induced mice challenged via inhaled and intranasal routes
-
Kim DI, Song MK, Lee K ,
-
BMC Pulm Med ,
-
2019,
-
19(1):241
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Several studies on the impact of cell culture parameters on the profile of host cell protein (HCP) impurities have been carried out in shake flasks. Herein, we explore how transferable the findings and conclusions of such investigations are to lab-scale...
View Publication25(OH)D3 and 1.25(OH)2D3 inhibits TNF-α expression in human monocyte derived macrophages
-
Rafique A, Rejnmark L, Heickendorff L, Møller HJ ,
-
PLoS One ,
-
2019,
-
14(4):e0215383
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Finding targetable gene fusions can expand the limited treatment options in radioactive iodine-refractory (RAI-r) thyroid cancer. To that end, we established a novel cell line ‘JVE404′ derived from an advanced RAI-r papillary thyroid cancer (PTC) patient, harboring an EML4-ALK gene...
View PublicationExpression of CRBN, IKZF1, and IKZF3 does not predict lenalidomide sensitivity and mutations in the cereblon pathway are infrequent in multiple myeloma
-
Dimopoulos K, Fibiger Munch-Petersen H, Winther Eskelund C, et al. ,
-
Leuk Lymphoma ,
-
2019,
-
60(1):180-188
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Chondrosarcomas are malignant cartilage tumors that are relatively resistant towards conventional therapeutic approaches. Kinase inhibitors have been investigated and shown successful for several different cancer types. In this study we aimed at identifying kinase inhibitors that inhibit the survival of...
View PublicationCHO cell cultures in shake flasks and bioreactors present different host cell protein profiles in the supernatant
-
Goey CH, Bell D, Kontoravdi C ,
-
Biochemical Engineering Journal ,
-
2019,
-
144:185–192
- Instrument: NucleoCounter® NC-250™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, Cell Vitality Assay
- Research area: Cell Biology
Purpose: Pseudomyogenic hemangioendothelioma (PHE) is an extremely rare locally aggressive neoplasm with endothelial differentiation, which often presents with multiple lesions. These tumors have characteristic SERPINE1-FOSB fusions. We report a 17 years old patient with advanced unresectable PHE with a durable...
View PublicationFanconi anemia with sun-sensitivity caused by a Xeroderma pigmentosum-associated missense mutation in XPF
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Popp I, Punekar M, Telford N, et al. ,
-
BMC Med Genet ,
-
2018,
-
19(1):7
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay, Cell Vitality Assay
- Research area: Cell-based Immunotherapy
Background: PD is a multisystem disease where both central and peripheral nervous systems are affected. This systemic involvement also includes the immune response in PD, which implicates not only microglia in the brain, but also peripheral immune cells, such as...
View PublicationAlterations in Blood Monocyte Functions in Parkinson’s Disease
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Nissen SK, Shrivastava K, Schulte C, et al. ,
-
Mov Disord ,
-
2019,
-
34(11):1711-1721
- Instrument: NucleoCounter® NC-250™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Recombinant Chinese hamster ovary (CHO) cells are able to provide biopharmaceuticals that are essentially free of human viruses and have N-glycosylation profiles similar, but not identical, to humans. Due to differences in N-glycan moieties, two members of the serpin superfamily,...
View PublicationGlyco-engineered CHO cell lines producing alpha-1-antitrypsin and C1 esterase inhibitor with fully humanized N-glycosylation profiles
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Amann T, Hansen AH, Kol S, et al. ,
-
Metab Eng ,
-
2019,
-
52:143-152
- Instrument: NucleoCounter® NC-250™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
In production of recombinant proteins for biopharmaceuticals, N-glycosylation is often important for protein efficacy and patient safety. IgG with agalactosylated (G0)-N-glycans can improve the activation of the lectin-binding complement system and be advantageous in the therapy of lupus and virus...
View PublicationCRISPR/Cas9-Multiplexed Editing of Chinese Hamster Ovary B4Gal-T1, 2, 3, and 4 Tailors N-Glycan Profiles of Therapeutics and Secreted Host Cell Proteins
-
Amann T, Hansen AH, Kol S, et al. ,
-
Biotechnol J ,
-
2018,
-
13(10):e1800111
- Instrument: NucleoCounter® NC-250™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
Bortezomib (BOZ) is a proteasome inhibitor chemotherapeutic agent utilized to treat multiple myeloma and recently offered to cure melanoma. Bortezomib-induced neuropathy is one of the dose-limiting side-effects, which can be treated with antioxidants (e.g. alpha-lipoic acid-ALA and Vitamin B1-vit B1)....
View PublicationFanconi anemia with sun-sensitivity caused by a Xeroderma pigmentosum-associated missensemutation in XPF
-
Popp I, Punekar M, Telford N, et al. ,
-
BMC Med Genet ,
-
2018,
-
19(1):7
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Diesel exhaust particulates (DEP) adversely affect the respiratory system and exacerbate lung diseases, resulting in high mortality rates. However, its pathogenesis is complicated, and the mechanisms involved are incompletely understood. We investigated the effects of DEP pre-exposure on lipopolysaccharide (LPS)-induced...
View PublicationCircadian clock component REV-ERBα controls homeostatic regulation of pulmonary inflammation
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Pariollaud M, Gibbs JE, Hopwood TW, et al. ,
-
J Clin Invest ,
-
2018,
-
128(6):2281-2296
- Instrument: NucleoCounter® NC-250™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Small Molecule Drugs
A hydrocyclone is a macroscale separation device employed in various industries, with many advantages, including high-throughput and low operational costs. Translating these advantages to microscale has been a challenge due to the microscale fabrication limitations that can be surmounted using...
View PublicationFunctional impairment of MSC induced by transient warming events: Correlation with loss of adhesion and altered cell size
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Chabot D, Lewin A, Loubaki L, Bazin R ,
-
Cytotherapy ,
-
2018,
-
20(8):990-1000
- Instrument: NucleoCounter® NC-250™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Levan is an exopolysaccharide produced by Bacillus licheniformis (strain FRI MY-55) that shows promising pharmacological activity. Phosphorylation is a chemical modification that can increase the biological and antioxidant properties of levan. In this study, levan was phosphorylated by microwave-assisted synthesis...
View PublicationBiallelic mutations in the ubiquitin ligase RFWD3 cause Fanconi anemia
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Knies K, Inano S, Ramírez MJ, et al. ,
-
J Clin Invest ,
-
2017,
-
127(8):3013-3027
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: We recently showed that transient warming effects decreased the functional and adhesion properties of mesenchymal stromal cells (MSC) while post-thaw viability remained high. In an attempt to better predict functional impairment of cryopreserved MSC, we further analysed the correlation...
View PublicationThe Oral Bioavailability of 8-Prenylnaringenin from Hops (Humulus Lupulus L.) in Healthy Womenand Men is Significantly Higher than that of its Positional Isomer 6-Prenylnaringenin in a Randomized Crossover Trial
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Calvo-Castro LA, Burkard M, Sus N, et al. ,
-
Mol Nutr Food Res ,
-
2018,
-
62(7):e1700838
- Instrument: NucleoCounter® NC-250™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Oncology
In this study, we investigated whether humidifier disinfectants (HDs) induce asthmatic airway inflammation in an animal model and compared the features of HD-induced inflammatory symptoms with ovalbumin (OVA)-induced allergic asthma. Mice were intratracheally instilled three times with either the control...
View PublicationMulti-stage population balance model to understand the dynamics of fed-batch CHO cell culture
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Alhuthali, Fadda S, Goey CH, Kontoravdi C ,
-
Computer Aided Chemical Engineering ,
-
2017,
-
40:2821–26
- Instrument: NucleoCounter® NC-250™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
Recent studies reveal that airway epithelial cells are critical pulmonary circadian pacemaker cells, mediating rhythmic inflammatory responses. Using mouse models, we now identify the rhythmic circadian repressor REV-ERBα as essential to the mechanism coupling the pulmonary clock to innate immunity,...
View PublicationTransient warming events occurring after freezing impairs umbilical cord–derived mesenchymal stromal cells functionality
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Chabot D, Tremblay T, Paré I, et al. ,
-
Cytotherapy ,
-
2017,
-
19(8):978-989
- Instrument: NucleoCounter® NC-250™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Fanconi anemia (FA) is an inherited genomic instability disorder with congenital and developmental abnormalities, bone marrow failure and predisposition to cancer early in life, and cellular sensitivity to DNA interstrand crosslinks. Case presentation: A fifty-one-year old female patient, initially...
View PublicationCascading effect in bioprocessing – The impact of mild hypothermia on CHO cell behaviour and host cell protein composition
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Goey CH, Tsang JMH, Bell D, Kontoravdi C ,
-
Biotechnol Bioeng ,
-
2017,
-
114(12):2771-2781
- Instrument: NucleoCounter® NC-250™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, Cell Vitality Assay
- Research area: Protein Production
Scope: Prenylated chalcones and flavonoids from hop (Humulus lupulus L.), such as 6-prenylnaringenin (6-PN) and 8-prenylnaringenin (8-PN), are investigated for their health beneficial and anticancer activities. We, thus, compare the oral bioavailability and safety of 6-PN and 8-PN in healthy...
View PublicationImproving cell viability using counterflow centrifugal elutriation
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Li A, Barabadi M, McDonald H, et al. ,
-
Cytotherapy ,
-
2022,
-
24(6):650-658
- Instrument: NucleoCounter® NC-202™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Cell viability is an important release criterion in the manufacturing of cell therapy products. Low cell viability can have significant impact on product quality and manufacturing efficiency. Counterflow centrifugation technology has been applied to the manufacturing of cell therapy...
View PublicationMapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq
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Replogle JM, Saunders RA, Pogson AN, et al. ,
-
Cell ,
-
2022,
-
185(14):2559-2575.e28
- Instrument: NucleoCounter® NC-202™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
A central goal of genetics is to define the relationships between genotypes and phenotypes. High-content phenotypic screens such as Perturb-seq (CRISPR-based screens with single-cell RNA-sequencing readouts) enable massively parallel functional genomic mapping but, to date, have been used at limited...
View PublicationAccelerating manufacturing to enable large-scale supply of a new adenovirus-vectored vaccine within 100 days
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Joe CCD, Segireddy RR, Oliveira C, et al. ,
-
bioRxiv ,
-
2021,
-
12.22.473478
- Instrument: NucleoCounter® NC-202™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Virus Production
The Coalition for Epidemic Preparedness Innovations’ ‘100-day moonshot’ aspires to launch a new vaccine within 100 days of pathogen identification. Here, we describe work to optimize adenovirus vector manufacturing for rapid response, by minimizing time to clinical trial and first...
View PublicationChitosan Membranes Filled with Cyclosporine A as Possible Devices for Local Administration of Drugs in the Treatment of Breast Cancer
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Trombino S, Curcio F, Poerio T, et al. ,
-
Molecules ,
-
2021,
-
26(7):1889
- Instrument: NucleoCounter® NC-202™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
The aim of this work is the design, preparation and characterization of membranes based on cyclosporine A (CsA) and chitosan carboxylate (CC) to be used as an implantable subcutaneous medical device for a prolonged therapeutic effect in the treatment of...
View PublicationEmulsion-templated microparticles with tunable stiffness and topology: Applications as edible microcarriers for cultured meat
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Norris SCP, Kawecki NS, Davis AR, et al. ,
-
Biomaterials ,
-
2022,
-
287:121669
- Instrument: NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cultured Meat
Cultured meat has potential to diversify methods for protein production, but innovations in production efficiency will be required to make cultured meat a feasible protein alternative. Microcarriers provide a strategy to culture sufficient volumes of adherent cells in a bioreactor...
View PublicationSingle-cell transcriptome and cell type-specific molecular pathways of human non-alcoholic steatohepatitis
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Fred RG, Steen Pedersen J, Thompson JJ, et al. ,
-
Sci Rep ,
-
2022,
-
12(1):13484
- Instrument: NucleoCounter® NC-200™
- Cell Type: Hepatocytes
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The aim of this study is to characterize cell type-specific transcriptional signatures in non-alcoholic steatohepatitis (NASH) to improve our understanding of the disease. We performed single-cell RNA sequencing on liver biopsies from 10 patients with NASH. We applied weighted gene...
View PublicationUpscaling human mesenchymal stromal cell production in a novel vertical-wheel bioreactor enhances extracellular vesicle secretion and cargo profile
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Jeske R, Liu C, Duke L, et al. ,
-
Bioactive Materials ,
-
2022,
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Human mesenchymal stromal cells (hMSCs) are mechanically sensitive undergoing phenotypic alterations when subjected to shear stress, cell aggregation, and substrate changes encountered in 3D dynamic bioreactor cultures. However, little is known about how bioreactor microenvironment affects the secretion and cargo...
View PublicationEnhancing Adoptive Cell Therapy by T Cell Loading of SHP2 Inhibitor Nanocrystals before Infusion
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Li X, Halldórsdóttir HR, Weller S, et al. ,
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Nano ,
-
2022,
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Whereas adoptive T cell therapy has been extensively studied for cancer treatment, the response is still limited primarily due to immune dysfunction related to poor cell engraftment, tumor infiltration and engagement, and lack of a target. In addition, the modification...
View PublicationParenchymal and Inflammatory Cell Responses to Single and Repeated Ozone Exposure in Healthy and Surfactant Protein-C Mutant Lung
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Nguyen J, Deering-Rice CE, Armstrong BS, et al. ,
-
Toxicol Sci ,
-
2022,
-
kfac074
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Mutations in the alveolar epithelial-specific gene encoding for surfactant protein C (SP-C) are linked to pulmonary disease. Ozone (O3) is a ubiquitous pollutant known to exacerbate stress through oxidative injury and inflammation. To comprehend the structural, functional, and immunological impact...
View PublicationClinical and laboratory findings following transplantation of allogeneic adipose-derived mesenchymal stromal cells in knee osteoarthritis, a brief report
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Sadri B, Tamimi A, Nouraein S, et al. ,
-
Connect Tissue Res ,
-
2022,
-
01/Dec
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
Background: Mesenchymal stromal cells (MSCs) injection has been proposed as an innovative treatment for knee osteoarthritis (KOA). Since, allogeneic MSCs can be available as off-the-shelf products, they are preferable in regenerative medicine. Among different sources for MSCs, adipose-derived MSCs (AD-MSCs)...
View PublicationEx Vivo Hydrostatic Pressure Loading of Atrial Tissues Activates Profibrotic Transcription via TGF-β Signal Pathway
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Zhang X, Yassouf Y, Huang K, et al. ,
-
Int Heart J. ,
-
2022,
-
63(2):367-374
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Excessive mechanical stress causes fibrosis-related atrial arrhythmia. Herein, we tried to investigate the mechanism of atrial fibrogenesis in response to mechanical stress by ex vivo approach. We collected atrial tissues from mice and then cultured them as “explants” under atmospheric...
View PublicationLongitudinal two-dimensional gas chromatography mass spectrometry as a non-destructive at-line monitoring tool during cell manufacturing identifies volatile features correlative to cell product quality
-
Jimenez AC, Heist CA, Navaei M, et al. ,
-
Cytotherapy ,
-
2022,
-
S1465-3249(22)00681-8
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background aims: Cell therapies have emerged as a potentially transformative therapeutic modality in many chronic and incurable diseases. However, inherent donor and patient variabilities, complex manufacturing processes, lack of well-defined critical quality attributes and unavailability of in-line or at-line process...
View PublicationMicrowell bag culture for large-scale production of homogeneous islet-like clusters
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Suenaga R, Konagaya S, Yamaura J, et al. ,
-
Sci Rep ,
-
2022,
-
12(1):5221
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Pluripotent stem-cell derived cells can be used for type I diabetes treatment, but we require at least 105-106 islet-like clusters per patient. Although thousands of uniform cell clusters can be produced using a conventional microwell plate, numerous obstacles need to...
View PublicationH3K9 dimethylation safeguards cancer cells against activation of the interferon pathway
-
Hansen AM, Ge Y, Schuster MB, et al. ,
-
Sci Adv ,
-
2022,
-
8(11):eabf8627
- Instrument: NucleoCounter® NC-200™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Activation of interferon genes constitutes an important anticancer pathway able to restrict proliferation of cancer cells. Here, we demonstrate that the H3K9me3 histone methyltransferase (HMT) suppressor of variegation 3-9 homolog 1 (SUV39H1) is required for the proliferation of acute myeloid...
View PublicationIn vitro culture at 39 °C during hepatic maturation of human ES cells facilitates hepatocyte-like cell functions
-
Imamura S, Yoshimoto K, Terada S, et al. ,
-
Sci Rep ,
-
2022,
-
12(1):5155
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Hepatocyte-like cells derived from human pluripotent stem cells (hPSC-HLCs) offer an alternative to primary hepatocytes commonly used for drug screenings and toxicological tests. However, these cells do not have hepatic functions comparable to those of hepatocytes in vivo due to...
View PublicationImmuno-reactive cancer organoid model to assess effects of the microbiome on cancer immunotherapy
-
Shelkey E, Oommen D, Stirling ER, et al. ,
-
Sci Rep ,
-
2022,
-
12(1):9983
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Immune checkpoint blockade (ICB) therapy has demonstrated good efficacy in many cancer types. In cancers such as non-resectable advanced or metastatic triple-negative breast cancer (TNBC), it has recently been approved as a promising treatment. However, clinical data shows overall response...
View PublicationAssociation of heat shock protein 8 with atopic march in a murine experimental model
-
Jeong KT, Do JH, Lee SH, et al. ,
-
PeerJ ,
-
2022,
-
10:e13247
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Atopic march (AM), a unique characteristic of allergic diseases, refers to the sequential progression of atopic dermatitis (AD) in infants to allergic asthma and allergic rhinitis in children and young adults, respectively. Although there are several studies on AM,...
View PublicationFractal Dimension and Texture Analysis in the Assessment of Experimental Laser-Induced Periodic Surface Structures (LIPSS) Dental Implant Surface—In Vitro Study Preliminary Report
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Hadzik J, Kubasiewicz-Ross P, Simka W, et al. ,
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Materials (Basel) ,
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2022,
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15(8):2713
- Instrument: NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Laser-induced periodic surface structures (LIPSS) are the sub-wavelength periodic nanostructures generated by the femtosecond laser. Implant topography and its nanostructural changes can be important for biomedical applications. In order to compare the surface topography of different implants, appropriate mathematical and...
View PublicationDesign and biofabrication of a leaf-inspired vascularized cell-delivery device
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Sämfors S, Niemi EM, Oskarsdotter K, et al. ,
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Bioprinting ,
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2022,
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26:e00199
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
We designed and biofabricated a channeled construct as a possible cell-delivery device that can be endothelialized to overcome size limitations due to oxygen diffusion. The channeled device mimicking a leaf was designed using computer-aided design software, with fluid flow through...
View PublicationExtracorporeal immune cell therapy of sepsis: ex vivo results
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Klinkmann G, Wild T, Heskamp B, et al. ,
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Intensive Care Med Exp ,
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2022,
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10(1):26
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Background: Immune cell dysfunction plays a central role in sepsis-associated immune paralysis. The transfusion of healthy donor immune cells, i.e., granulocyte concentrates (GC) potentially induces tissue damage via local effects of neutrophils. Initial clinical trials using standard donor GC in...
View PublicationQbD-guided pharmaceutical development of Pembrolizumab biosimilar candidate PSG-024 propelled to industry meeting primary requirements of comparability to Keytruda®
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Jaffar-Aghaei M, Khanipour F, Maghsoudi A, et al. ,
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Eur J Pharm Sci ,
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2022,
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173:106171
- Instrument: NucleoCounter® NC-200™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
Pharmaceutical development of biosimilars is primarily focused on meeting the regulatory requirements for analytical comparability of the product’s critical quality attributes (CQAs), concerning safety and efficacy, to those of the originator drug of interest. To this end, the early adoption...
View PublicationAnti-CTLA-4 and anti-PD-1 immunotherapies repress tumor progression in preclinical breast and colon model with independent regulatory T cells response
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Rupp T, Genest L, Babin D, et al. ,
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Transl Oncol ,
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2022,
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20:101405
- Instrument: NucleoCounter® NC-200™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
The recent development of immunotherapy represents a significant breakthrough in cancer therapy. Several immunotherapies provide robust efficacy gains in a wide variety of cancers. However, in some patients the immune checkpoint blockade remains ineffective due to poor therapeutic response and...
View PublicationInhibition of lung microbiota-derived proapoptotic peptides ameliorates acute exacerbation of pulmonary fibrosis
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D'Alessandro-Gabazza CN, Yasuma T, Kobayashi T, et al. ,
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Nat Commun ,
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2022,
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13(1):1558
- Instrument: NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
Idiopathic pulmonary fibrosis is an incurable disease of unknown etiology. Acute exacerbation of idiopathic pulmonary fibrosis is associated with high mortality. Excessive apoptosis of lung epithelial cells occurs in pulmonary fibrosis acute exacerbation. We recently identified corisin, a proapoptotic peptide...
View PublicationExposure of human immune cells, to the antiretrovirals efavirenz and lopinavir, leads to lower glucose uptake and altered bioenergetic cell profiles through interactions with SLC2A1
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Heaton BJ, Jensen RL, Line J, et al. ,
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Biomed Pharmacother ,
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2022,
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150:112999
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
SLC2A1 mediates glucose cellular uptake; key to appropriate immune function. Our previous work has shown efavirenz and lopinavir exposure inhibits T cell and macrophage responses, to known agonists, likely via interactions with glucose transporters. Using human cell lines as a...
View PublicationStromal Vascular Fraction and Mesenchymal Stem Cells from Human Adipose Tissue: A Comparison of Immune Modulation and Angiogenic Potential
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Tran TDX, Pham VQ, Tran NN, et al. ,
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Adv Exp Med Biol ,
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2022,
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Introduction: In recent years, both stromal vascular fraction (SVF) from adipose tissue and mesenchymal stem cells (MSC) from adipose tissues were extensively used in both preclinical and clinical treatment for various diseases. Some studies reported differences in treatment efficacy between...
View PublicationCD206+ tumor-associated macrophages cross-present tumor antigen and drive antitumor immunity
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Modak M, Mattes AK, Reiss D, et al. ,
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JCI Insight ,
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2022,
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7(11):e155022
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
In many solid cancers, tumor-associated macrophages (TAM) represent the predominant myeloid cell population. Antigen (Ag) cross-presentation leading to tumor Ag-directed cytotoxic CD8+ T cell responses is crucial for antitumor immunity. However, the role of recruited monocyte-derived macrophages, including TAM, as...
View PublicationOptimization of a PDMS-Based Cell Culture Substrate for High-Density Human-Induced Pluripotent Stem Cell Adhesion and Long-Term Differentiation into Cardiomyocytes under a Xeno-Free Condition
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Etezadi F, Le MNT, Shahsavarani H, et al. ,
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ACS Biomater Sci Eng ,
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2022,
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8(5):2040-2052
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Despite the numerous advantages of PDMS-based substrates in various biomedical applications, they are limited by their highly hydrophobic surface that does not optimally interact with cells for attachment and growth. Hence, the lack of lengthy and straightforward procedures for high-density...
View PublicationTreatment of Abdominal Aortic Aneurysm Utilizing Adipose-Derived Mesenchymal Stem Cells in a Porcine Model
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Zilberman B, Kooragayala K, Lou J, et al. ,
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J Surg Res ,
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2022,
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278:247-256
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cardiology
Introduction: The current treatment paradigm of abdominal aortic aneurysms (AAA) focuses on observing patients until their disease reaches certain thresholds for intervention, with no preceding treatment available. There is an opportunity to develop novel therapies to prevent further aneurysmal growth...
View PublicationScalable expansion of iPSC and their derivatives across multiple lineages
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Kwok CK, Sébastien I, Hariharan K, et al. ,
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Reprod Toxicol ,
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2022,
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112:23-35
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Induced pluripotent stem cell (iPSC) technology enabled the production of pluripotent stem cell lines from somatic cells from a range of known genetic backgrounds. Their ability to differentiate and generate a wide variety of cell types has resulted in their...
View PublicationLarge-scale engineering of hiPSC-derived nephron sheets and cryopreservation of their progenitors
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Wiersma LE, Avramut MC, Lievers E, et al. ,
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Stem Cell Res Ther ,
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2022,
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13(1):208
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Background: The generation of human induced pluripotent stem cells (hiPSCs) has opened a world of opportunities for stem cell-based therapies in regenerative medicine. Currently, several human kidney organoid protocols are available that generate organoids containing kidney structures. However, these kidney...
View PublicationA Platform of Patient-Derived Microtumors Identifies Individual Treatment Responses and Therapeutic Vulnerabilities in Ovarian Cancer
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Anderle N, Koch A, Gierke B, et al. ,
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Cancers ,
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2022,
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14(12):2895
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
In light of the frequent development of therapeutic resistance in cancer treatment, there is a strong need for personalized model systems representing patient tumor heterogeneity, while enabling parallel drug testing and identification of appropriate treatment responses in individual patients. Using...
View PublicationStandard toxicity study of clinical-grade allogeneic human bone marrow-derived clonal mesenchymal stromal cells
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Tayebi B, Babaahmadi M, Pakzad M, et al. ,
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Stem Cell Res Ther ,
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2022,
-
13(1):213
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Introduction: Mesenchymal stromal cells (MSCs) have opened a new window to treat inflammatory and non-inflammatory diseases. Nonetheless, their clinical applications require rigorous control and monitoring procedures to ensure full compliance with the principles of good manufacturing practice (GMP). Various evaluations...
View PublicationEnhancing CHO cell productivity through a dual selection system using Aspg and Gs in glutamine free medium
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Ha T, Òdena A, Karottki KJlC, et al. ,
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Authorea ,
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2022,
- Instrument: NucleoCounter® NC-200™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
The dominant method for generating Chinese hamster ovary (CHO) cell lines that produce high titers of biotherapeutic proteins utilizes selectable markers such as dihydrofolate reductase (Dhfr) or glutamine synthetase (Gs), alongside inhibitory compounds like methotrexate (MTX) or methionine sulfoximine (MSX),...
View PublicationYAP activates pancreatic stellate cells and enhances pancreatic fibrosis
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Spanehl L, Revskij D, Bannert K, et al. ,
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Hepatobiliary & Pancreatic Diseases International ,
-
2022,
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background Pancreatic stellate cells (PSCs) foster the progression of pancreatic adenocarcinoma and chronic pancreatitis (CP) by producing a dense fibrotic stroma. However, the incomplete knowledge of PSCs biology hampers the exploration of antifibrotic therapies. Here, we explored the role of...
View Publication