Cell Count Assay
Detection of Total and Dead Cell Populations Using Fluorescent Cytometry
- Fast and convenient cell count and viability measurement
- Consistency across instruments, users and sites
- Objective analysis – no human error in sample preparation
- Scalable system with 21 CFR Part 11 / GMP-ready software
- Personal support by our dedicated experts
- Specialized protocols for mammalian and insect cells
- Automated PDF reports
- Export data in FCS/ACS formats (for NC-3000™)
Overview of the Cell Count & Viability Assay
With the NucleoCounter® family of cell counters, you can count your cells quickly and easily. Our cell counting assays are accurate, precise, and versatile, meeting the needs of even the most specialized cell types and cultures.
We offer several instruments with cell counting assays that are optimized for different applications:
- NucleoCounter®NC-202™ for straightforward cell counting in all cell cultures
- NucleoCounter®NC-3000™ combines cell counting with advanced image cytometry
- NucleoCounter®SP-100™ for sperm cells
- NucleoCounter®YC-100™ for yeast cells
- NucleoCounter®SCC-100™ for somatic cells
How Our Cell Count Assays Work
Our NucleoCounter® instruments use automated fluorescence cytometry to count cells and determine viability. They rely on fluorescent dyes, acridine orange (AO) and 4′,6-diamidino-2-phenylindole (DAPI), to identify live and dead cells and provide accurate cell counts.
AO is a membrane-permeable dye that binds to nucleic acids, staining all the cells in a sample. DAPI cannot penetrate the cell membrane, hence it only stains non-viable cells with a damaged cell membrane. Detecting the signals from these two fluorescent dyes allows us to exclude debris and count cells accurately and consistently, with no subjectivity about what is or is not counted as a cell.
Trusted by Experts
Thanks to their versatility, our cell counting instruments are used by thousands of customers around the globe in a vast range of applications, including cell and gene therapy, therapeutic protein production, vaccine manufacturing, bioprocessing, clean meat, animal reproduction, and research. Some recently published examples include:
- Researchers at the Jenner Institute used a NucleoCounter® to monitor the cell density of their HEK cells during their adenovirus-vectored vaccine manufacturing processes. Their work was published recently on bioRxiv.
- Research from Theresienkrankenhaus showed that in patients with osteoarthritis of the knee who received adipogenic stromal-vascular fraction therapy, a greater number of cells (measured with a NucleoCounter®) correlated with better outcomes for patients.
- Clinical trials of Yescarta, an autologous CAR T-cell therapy for lymphoma which is now FDA approved, used NucleoCounter® instruments to monitor therapy cell densities.
- The scientists at Mosa Meat used a NucleoCounter® to monitor the production of fat cells for cultured meat.
You can read more examples of our cell counting assays in action on our customer projects page.
The Advantages of NucleoCounter® Cell Counting
Precise, Objective Analysis
Precise, repeatable results mean confidence in your processes, research, and decisions. Our cell counting assays use a comprehensive approach to eliminate human error and guarantee precise, objective cell counting:
- We use fluorescence microscopy and image analysis to quantify cells automatically. NucleoCounter® instruments used fixed focus and exposure, so no adjustments are required, removing the potential for human error or inter-user variation.
- All our instruments are calibrated against a reference instrument, ensuring that every machine gives the same result, no matter where they are or who is operating them.
- Our cassettes are individually volume calibrated to ensure high precision. Furthermore, they contain immobilized cell counting dyes, making staining a closed, standardized process that eliminates pipetting errors.
Our software performs a series of analysis checks to ensure optimal sample analysis and will tell you if there are too few or too many cells in the sample to ensure accurate results.
Our instruments, cassettes, and software combine to provide exact cell counting and low coefficients of variation, no matter who does the analysis, where they do it, or which instrument they use. As a result, our NucleoCounter® instruments provide lower variation than other cell counting assays. For example, the NC-202™ typically provides a coefficient of variation below 5%.
Fast & Easy to Use
Our cell count assays offer fast and easy-to-use workflows. For example, if you are using the NucleoCounter® NC-202™, you simply place the built-in pipette of your Via2-cassette™ into your sample and press the piston to draw the liquid up into the cassette.
You then put the cassette into your NucleoCounter® and press ‘run’ to start the ‘Cell Count & Viability Assay,’ our universal cell counting protocol. Your results are displayed in less than 30 seconds.
Easy GMP-Compliance
Our protocols eliminate variability and subjectivity from the cell counting process, ensuring that your results are precise and consistent, no matter who is conducting the count. What’s more, our simple workflows are ideal for short, easy to understand SOPs. Consequently, validating your cell counting methods for GMP compliance is straightforward and pain-free. What’s more, our NC-200™, NC-202™, NC-250™, and NC-3000™ instruments are Annex-11 and 21 CFR Part 11-ready.
Count All Cell Types
Our cell counting assays are universal and can count all nucleated cells. As a result, you can use our NucleoCounter® instruments with even the most specialized cell types and culture conditions, including:
Primary mammalian cells | Natural killer (NK) cells |
Leukocytes | Insect cells |
Chinese hamster ovary (CHO) cells | Yeast |
Mesenchymal stem cells (MSCs) | Whole blood (WBC) |
Induced pluripotent stem cells (iPSCs) | Stromal vascular fractions |
Peripheral blood mononuclear cells (PBMCs) | Microcarriers |
Hepatocytes | Spheroids |
T cells | Aggregated cells |
Specialist Support
Our NucleoCounter® instruments all come with access to our scientists and cell counting experts. Our world-class team will support you throughout product installation and protocol development. We also offer training, application support, and dedicated field service teams to ensure you achieve optimal instrument performance and the best quality results.
To learn more about our cell counting solutions, visit our products page or contact us.
Instrument Solutions
Tech notes
Publications
Study on prevalence and bacterial etiology of mastitis, and effects of subclinical mastitis and stage of lactation on SCC in dairy goats in Egypt
-
Hussein HA, Fouad MT, Abd El-Razik KA, et al. ,
-
Tropical Animal Health and Production ,
-
2020,
-
52(6):3091-3097
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Dairy (SCC)
In Egypt, inadequate information on prevalence and epidemiology of caprine mastitis is available. This study was designed to investigate prevalence and etiological agents of caprine mastitis and assess the efficacy of somatic cell count (SCC) as marker of subclinical mastitis...
View PublicationRelationship between Somatic Cell Count and Different Microbial and Chemical Quality Parameters of Bulk Tank Milk
-
El-Shaimaa MN, El-Shafaie MA, Fahim KM, Sallam SS ,
-
Advances in Animal and Veterinary Sciences ,
-
2021,
-
9(10): 1660-1668
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Dairy (SCC)
The objective of this study was to investigate bulk tank somatic cell count and associated risk factors influencing the hygienic quality and chemical composition of raw milk; additionally, to assess the impact of somatic cell count (SCC/ml) on the microbial...
View PublicationCrude coriander oil in the diet of lactating goats enhanced lactational performance, ruminal fermentation, apparent nutrient digestibility, and blood chemistry
-
Kholif AE, Elazab MA, Matloup OH, et al. ,
-
Small Ruminant Research ,
-
2021,
-
204(106522):0921-4488
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Dairy (SCC)
The use of safe feed additives in diets of lactating animals has gained increasing interest. The effects of feeding lactating goats on diet supplemented with crude coriander oil at different levels were evaluated. Thirty multiparous lactating Damascus × Baladi goats...
View PublicationNovel Biomarkers of Mastitis in Goat Milk Revealed by MALDI-TOF-MS-Based Peptide Profiling
-
Matuozzo M, Spagnuolo MS, Hussein HA, et al. ,
-
Biology ,
-
2020,
-
9(8):193
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Dairy (SCC)
Mastitis is the most common infection of dairy goats impairing milk production and quality, which is usually recognized by mammary gland visual inspection and palpation. Subclinical forms of the disease are also widely represented, which lack the typical signs of...
View PublicationAn impedimetric immunosensor for the selective detection of CD34+ T-cells in human serum
-
Díaz-Fernández A, Bernalte E, Fernández-Ramos C, et al. ,
-
Sensors and Actuators B: Chemical ,
-
2022,
-
356(131306)
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Oncology
Chimeric antigen receptor T (CAR-T) cell therapy has led to a paradigm change in cancer treatment. To allow widespread use of this therapy, it is important to reduce the costs associated with cells manufacturing whilst ensuring a high-quality production. Online...
View PublicationInfiltration of the Hoffa’s fat pad in patients with osteoarthritis of the knee-Results after one year of follow-up
-
Labarre KW, Zimmermann G ,
-
Bone Reports ,
-
2022,
-
101168
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Cell therapy using multipotential stromal cells (MSCs) is being used in a variety of clinical settings to induce tissue regeneration. Promising results have also been achieved in the therapy of osteoarthritis. MSCs have been demonstrated to be safe (Borakati et...
View PublicationAdipose-Derived Stem Cells from Type 2 Diabetic Rats Retain Positive Effects in a Rat Model of Erectile Dysfunction
-
Quaade ML, Dhumale P, Steffensen SGC, et al. ,
-
International Journal of Molecular Sciences ,
-
2022,
-
23(3):1692
- Cell Type: MSCs
- Assay: Cell Count Assay
- Research area: Stem Cell Therapy
Erectile dysfunction is a common complication associated with type 2 diabetes mellitus (T2DM) and after prostatectomy in relation to cancer. The regenerative effect of cultured adipose-derived stem cells (ASCs) for ED therapy has been documented in multiple preclinical trials as...
View PublicationAutologous Induced Pluripotent Stem Cell-Based Cell Therapies: Promise, Progress, and Challenges
-
Madrid M, Sumen C, Aivio S, Saklayen N ,
-
Curr Protoc ,
-
2021,
-
1(3):e88
- Assay: Cell Count Assay
- Research area: Stem Cell Therapy
The promise of human induced pluripotent stem cells (iPSCs) lies in their ability to serve as a starting material for autologous, or patient-specific, stem cell-based therapies. Since the first publications describing the generation of iPSCs from human tissue in 2007,...
View PublicationOLFM4-RET fusion is an oncogenic driver in small intestine adenocarcinoma
-
Liu W, Li H, Aerbajinai W, et al. ,
-
Oncogene ,
-
2021,
- Cell Type: Epithelial Cells
- Assay: Cell Count Assay
- Research area: Stem Cell Research
Inflammation early in life can prime the local immune milieu of peripheral tissues, which can cause lasting changes in immunological tone that confer disease protection or susceptibility1. The cellular and molecular mechanisms that prompt changes in immune tone in many...
View PublicationPreparation and growth factor characterization of cord blood-derived plasma, serum, growth factor-rich plasma and induced serum
-
Rhéaume ME, Perreault J, Fournier D, Trépanier P ,
-
Cytokine ,
-
2022,
-
149:155756
- Cell Type: Whole Blood
- Assay: Cell Count Assay
- Research area: Cell Biology
Small intestine adenocarcinoma is a rare intestinal malignancy with distinct clinical, pathological, and molecular characteristics. Recently, a fusion of the intestinal stem-cell marker olfactomedin 4 (OLFM4) and the proto-oncogene RET has been identified in a small intestine adenocarcinoma patient. Here...
View PublicationMuscle-derived fibro-adipogenic progenitor cells for production of cultured bovine adipose tissue
-
Dohmen RGJ, Hubalek S, Melke J, et al. ,
-
NPJ Sci Food ,
-
2022,
-
6(1):6
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Cultured Meat
Cultured meat is an emergent technology with the potential for significant environmental and animal welfare benefits. Accurate mimicry of traditional meat requires fat tissue; a key contributor to both the flavour and texture of meat. Here, we show that fibro-adipogenic...
View PublicationOptimizing large-scale autologous human keratinocyte sheets for major burns—Toward an animal-free production and a more accessible clinical application
-
Frese L, Darwiche SE, Gunning ME, et al. ,
-
Health Sci Rep ,
-
2022,
-
5(1):e449
- Cell Type: Epithelial Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Background and aims: Autologous keratinocyte sheets constitute an important component of the burn wound treatment toolbox available to a surgeon and can be considered a life-saving procedure for patients with severe burns over 50% of their total body surface area....
View PublicationExposure to Nickel Oxide Nanoparticles Induces Acute and Chronic Inflammatory Responses in Rat Lungs and Perturbs the Lung Microbiome
-
Jeong MJ, Jeon S, Yu HS, et al. ,
-
Int J Environ Res Public Health ,
-
2022,
-
19(1):522
- Cell Type: Immune Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Nickel oxide nanoparticles (NiO NPs) are highly redox active nanoparticles. They can cause acute and chronic inflammation in rat lungs. Unlike the gut microbiome, the association between the lung microbiome’s role and pulmonary inflammatory response to inhaled nanoparticles remains largely...
View PublicationLong-term in vivo survival of 3D-bioprinted human lipoaspirate-derived adipose tissue: proteomic signature and cellular content
-
Säljö K, Apelgren P, Stridh Orrhult L, et al. ,
-
Adipocyte ,
-
2022,
-
11(1):34-46
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Three-dimensional (3D)-bioprinted lipoaspirate-derived adipose tissue (LAT) is a potential alternative to lipo-injection for correcting soft-tissue defects. This study investigated the long-term in vivo survival of 3D-bioprinted LAT and its proteomic signature and cellular composition. We performed proteomic and multicolour flow...
View PublicationDevelopment of a human umbilical cord-derived mesenchymal stromal cell-based advanced therapy medicinal product to treat immune and/or inflammatory diseases
-
Mebarki M, Iglicki N, Marigny C, et al. ,
-
Stem Cell Research & Therapy ,
-
2021,
-
0,896527778
- Cell Type: MSCs
- Assay: Cell Count Assay
- Research area: Cell-based Immunotherapy
Background Umbilical cord-derived mesenchymal stromal cells (UC-MSCs) revealed their key role in immune regulation, offering promising therapeutic perspectives for immune and inflammatory diseases. We aimed to develop a production process of an UC-MSC-based product and then to characterize UC-MSC properties...
View PublicationProlonged storage of purified granulocyte concentrates: Introduction of a new purification method
-
Klinkmann G, Doss F, Goudeva L, et al. ,
-
Transfusion ,
-
2021,
- Cell Type: PBMCs
- Assay: Cell Count Assay
- Research area: Cell Biology
Background: Use of donor granulocyte concentrate (GC) has been limited due to its short storage time of 6-24 h, which is partially due to residual red blood cells (RBCs) and platelets and the resulting lactate production leading to an acidotic...
View PublicationProton-transfer-reaction mass spectrometry (PTR-MS) for online monitoring of glucose depletion and cell concentrations in HEK 293 gene therapy processes
-
Bayer B, Maccani A, Jahn J, et al. ,
-
Biotechnol Lett ,
-
2021,
- Cell Type: Epithelial Cells
- Assay: Cell Count Assay
- Research area: Gene Therapy
Objectives: The applicability of proton-transfer-reaction mass spectrometry (PTR-MS) as a versatile online monitoring tool to increase consistency and robustness for recombinant adeno-associated virus (rAAV) producing HEK 293 bioprocesses was evaluated. We present a structured workflow to extract process relevant information...
View PublicationSingle-cell RNA-seq of out-of-thaw mesenchymal stromal cells shows tissue-of-origin differences and inter-donor cell-cycle variations
-
Medrano-Trochez C, Chatterjee P, Pradhan P, et al. ,
-
Stem Cell Res Ther ,
-
2021,
-
12(1):565
- Cell Type: MSCs
- Assay: Cell Count Assay
- Research area: Cell Biology
Preparation and growth factor characterization of cord blood-derived plasma, serum, growth factor-rich plasma and induced serum
View PublicationNanomaterial- and shape-dependency of TLR2 and TLR4 mediated signaling following pulmonary exposure to carbonaceous nanomaterials in mice
-
Danielsen PH, Bendtsen KM, Knudsen KB, et al. ,
-
Part Fibre Toxicol ,
-
2021,
-
18(1):40
- Cell Type: Immune Cells
- Assay: Cell Count Assay
- Research area: Cell-based Immunotherapy
Background: Human Mesenchymal stromal cells (hMSCs) from various tissue sources are widely investigated in clinical trials. These MSCs are often administered to patients immediately after thawing the cryopreserved product (out-of-thaw), yet little is known about the single-cell transcriptomic landscape and...
View PublicationA double-blinded placebo-controlled evaluation of adipose-derived mesenchymal stem cells in treatment of canine atopic dermatitis
-
Kaur G, Ramirez A, Xie C, et al. ,
-
Vet Res Commun ,
-
2021,
- Cell Type: MSCs
- Assay: Cell Count Assay
- Research area: Cell-based Immunotherapy
Background: Pulmonary exposure to high doses of engineered carbonaceous nanomaterials (NMs) is known to trigger inflammation in the lungs paralleled by an acute phase response. Toll-like receptors (TLRs), particularly TLR2 and TLR4, have recently been discussed as potential NM-sensors, initiating...
View PublicationEarly-life inflammation primes a T helper 2 cell-fibroblast niche in skin
-
Boothby IC, Kinet MJ, Boda DP, et al. ,
-
Nature ,
-
2021,
-
599(7886):667-672
- Cell Type: Immune Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Mesenchymal stem cells (MSCs) have emerged as a new therapy for various immune-mediated inflammatory diseases. In this study we perform the first double-blinded, placebo-controlled evaluation of the efficacy of adipose-derived allogenic canine MSCs for the treatment of canine atopic dermatitis...
View PublicationHuman-derived Treg and MSC combination therapy may augment immunosuppressive potency in vitro, but did not improve blood brain barrier integrity in an experimental rat traumatic brain injury model
-
Caplan HW, Prabhakara KS, Toledano Furman NE, et al. ,
-
PLoS One ,
-
2021,
-
16(5):e0251601
- Cell Type: Immune Cells
- Assay: Cell Count Assay
- Research area: Cell-based Immunotherapy
Traumatic brain injury (TBI) causes both physical disruption of the blood brain barrier (BBB) and altered immune responses that can lead to significant secondary brain injury and chronic inflammation within the central nervous system (CNS). Cell therapies, including mesenchymal stromal...
View PublicationMSC Manufacturing for Academic Clinical Trials: From a Clinical-Grade to a Full GMP-Compliant Process
-
Lechanteur C, Briquet A, Bettonville V, et al. ,
-
Cells ,
-
2021,
-
10(6):1320
- Cell Type: MSCs
- Assay: Cell Count Assay
- Research area: Stem Cell Research
Following European regulation 1394/2007, mesenchymal stromal cell (MSCs) have become an advanced therapy medicinal product (ATMP) that must be produced following the good manufacturing practice (GMP) standards. We describe the upgrade of our existing clinical-grade MSC manufacturing process to obtain...
View PublicationSupporting dataset of two integration-free induced pluripotent stem cell lines from related human donors
-
Ramme AP, Faust D, Koenig L, et al. ,
-
Data Brief ,
-
2021,
-
37:107140
- Assay: Cell Count Assay
- Research area: Cell Biology
Integration-free induced pluripotent stem cells from related human donors’ exhibit great potential to the ongoing development of organ models. Blood cells from two different human donors were isolated, purified and reprogrammed into induced pluripotent stem cells. These induced pluripotent stem...
View PublicationA Novel Method to Gently Mix and Uniformly Suspend Particulates for Automated Assays
-
Myers KK, Herich JP, Chavez JE, et al. ,
-
SLAS Technol ,
-
2021,
-
26(5):498-509
- Cell Type: Epithelial Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
The SpinVessel system provides a methodology using pulsed radial flow to gently mix and uniformly suspend particulates (cells, magnetic beads, silica beads, and microcarrier beads) for automated assays. SpinVessels are well suited for aliquoting on robotic liquid handlers and with...
View PublicationHypoxia drives murine neutrophil protein scavenging to maintain central carbon metabolism
-
Watts ER, Howden AJ, Morrison T, et al. ,
-
J Clin Invest ,
-
2021,
-
131(10):e134073
- Cell Type: Immune Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Limiting dysfunctional neutrophilic inflammation while preserving effective immunity requires a better understanding of the processes that dictate neutrophil function in the tissues. Quantitative mass-spectrometry identified how inflammatory murine neutrophils regulated expression of cell surface receptors, signal transduction networks, and metabolic...
View PublicationImpact of lipopolysaccharides on cultivation and recombinant protein expression in human embryonal kidney (HEK‐293) cells
-
Faust C, Beil C, Dittrich W, et al. ,
-
Eng Life Sci ,
-
2021,
-
21(11):778-785
- Cell Type: Epithelial Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
The human embryonal kidney 293 cell (HEK-293) is a widely used expression host for transient gene expression. The genes or plasmids used for the transient transfections are usually propagated and extracted from the gram-negative bacterium Escherichia coli, the workhorse for...
View PublicationThermal proteome profiling identifies PIP4K2A and ZADH2 as off-targets of Polo-like kinase 1 inhibitor volasertib
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Goroshchuk O, Kolosenko I, Kunold E, et al. ,
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FASEB J ,
-
2021,
-
35(7):e21741
- Cell Type: Immune Cells
- Assay: Cell Count Assay
- Research area: Oncology
Polo-like kinase 1 (PLK1) is an important cell cycle kinase and an attractive target for anticancer treatments. An ATP-competitive small molecular PLK1 inhibitor, volasertib, has reached phase III in clinical trials in patients with refractory acute myeloid leukemia as a...
View PublicationSmall Intestinal Tuft Cell Activity Associates With Energy Metabolism in Diet-Induced Obesity
-
Arora P, Andersen D, Moll JM, et al. ,
-
Front Immunol ,
-
2021,
-
12:629391
- Cell Type: Epithelial Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Little is known about the involvement of type 2 immune response-promoting intestinal tuft cells in metabolic regulation. We here examined the temporal changes in small intestinal tuft cell number and activity in response to high-fat diet-induced obesity in mice and...
View PublicationInflammatory Response, Reactive Oxygen Species Production and DNA Damage in Mice After Intrapleural Exposure to Carbon Nanotubes
-
Wils RS, Jacobsen NR, Vogel U, et al. ,
-
Toxicol Sci ,
-
2021,
-
183(1):184-194
- Cell Type: Immune Cells
- Assay: Cell Count Assay
- Research area: Oncology
Carbon nanotubes (CNTs) are speculated to cause mesothelioma by persistent inflammation, oxidative stress, tissue injury, and genotoxicity. To investigate the pleural response to CNTs, we exposed C57BL/6 mice by intrapleural injection of 0.2 or 5 µg multiwalled CNTs (MWCNT-7, NM-401,...
View PublicationImpact of allogeneic feline uterine-derived mesenchymal stromal cell intravenous treatment on renal function of nephrectomized cats with chronic kidney disease
-
Zacharias S, Welty MB, Sand TT, Black LL ,
-
Research in Veterinary Science ,
-
2021,
-
2021 Sep 30
- Cell Type: MSCs
- Assay: Cell Count Assay
- Research area: Stem Cell Therapy
Chronic kidney disease (CKD) is a common condition and leading cause of mortality in cats. Mesenchymal stromal cells (MSCs) may have a therapeutic effect on CKD. The aim of this pilot study was to determine efficacy of systemically-administered allogeneic uterine...
View PublicationExtracellular vesicles from adipose stromal cells combined with a thermoresponsive hydrogel prevent esophageal stricture after extensive endoscopic submucosal dissection in a porcine model
-
Coffin E, Grangier A, Perrod G, et al. ,
-
Nanoscale ,
-
2021,
-
13(35):14866-14878
- Cell Type: Immune Cells
- Assay: Cell Count Assay
- Research area: Cell-based Immunotherapy
In this study, we investigated the combination of extracellular (nano) vesicles (EVs) from pig adipose tissue-derived stromal cells (ADSCs) and a thermoresponsive gel, Pluronic® F-127 (PF-127), to prevent stricture formation after endoscopic resection in a porcine model. ADSC EVs were...
View PublicationDesensitization of human breast progenitors by a transient exposure to pregnancy levels of estrogen
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Rønnov-Jessen L, Kim J, Goldhammer N, et al. ,
-
Sci Rep ,
-
2021,
-
11(1):17232
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Full term pregnancy at an early age is the only factor known to consistently protect against breast cancer. Because hormone receptor positive progenitors in the human breast relay endocrine signaling, we here sought to determine whether an experimental mimicry of...
View PublicationHuman Adipose Derived Stem Cells Enhance Healing in a Rat Model of Esophageal Injury with Stent
-
McCloskey D, Linden K, Lin A, et al. ,
-
J Surg Res ,
-
2021,
-
267:458-466
- Cell Type: MSCs
- Assay: Cell Count Assay
- Research area: Cell Biology
Background: Mesenchymal stem cells have been proven to promote cellular recruitment and remodeling during healing. Considering challenges encountered in the healing process of esophageal injury, we sought to evaluate the effect of human adipose derived stem cells (hASC) on esophageal...
View PublicationFlow Cytometric Analysis Revealed a Significant Accumulation of Terminally Differentiated T cell Subtypes in the Circulating Lymphocytes of Cytomegalovirus (CMV) Positive Follicular Lymphoma Patients
-
Lugos MD, Dangana A, Ntuhun BD, et al. ,
-
Journal of Clinical & Biomedical Research ,
-
2021,
-
01/Sep
- Cell Type: PBMCs
- Assay: Cell Count Assay
- Research area: Oncology
Follicular lymphoma (FL), a non-Hodgkin lymphoma, is an indolent cancer of the B cell lineage that runs a chronic deterioration course that can result in multiple treatment episodes leading to resistance and possible transformation to diffuse large B cell lymphoma....
View PublicationDicentracin-Like from Asian sea bass Fish and Moronecidine-Like from Hippocampus Comes: Two Candidate Antimicrobial Peptides Against Leishmanina major Infection
-
Mohammadi M, Moradi Hasan-Abad A, Dehghani P, et al. ,
-
International Journal of Peptide Research and Therapeutics ,
-
2021,
-
27:769-778
- Cell Type: Immune Cells
- Assay: Cell Count Assay
- Research area: Immunology
Anti-Leishmanial drug therapy faces significant challenges related to cytotoxicity and drug resistance. Thus, new and efficient anti-Leishmanial drugs need to be identified. Due to their broad-spectrum antimicrobial and also immunomodulatory activities, antimicrobial peptides (AMPs) have attracted considerable attention. In this...
View PublicationCholesterol loading suppresses the atheroinflammatory gene polarization of human
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Lappalainen J, Yeung N, Nguyen SD, et al. ,
-
Scientific Reports ,
-
2021,
-
11(1):4923
- Cell Type: Immune Cells
- Assay: Cell Count Assay
- Research area: Immunology
In atherosclerotic lesions, blood-derived monocytes differentiate into distinct macrophage subpopulations, and further into cholesterol-filled foam cells under a complex milieu of cytokines, which also contains macrophage-colony stimulating factor (M-CSF) and granulocyte-macrophage-colony stimulating factor (GM-CSF). Here we generated human macrophages in...
View PublicationTherapeutic potential of Fingolimod in triple negative breast cancer preclinical models
-
Rupp T, Pelouin O, Genest L, et al. ,
-
Translational Oncology ,
-
2021,
-
14(1):100926
- Cell Type: Cancer Cells
- Assay: Cell Count Assay
- Research area: Oncology
Surgery followed by a chemotherapy agent is the first-line treatment for breast cancer patients. Nevertheless, new targets are required for women with triple-negative breast cancer (TNBC) in order to improve the treatment of this aggressive cancer subtype. Multiple pro-inflammatory molecules...
View PublicationAntihormone treatment differentially regulates PSA secretion, PSMA expression and 68Ga–PSMA uptake in LNCaP cells
-
Mathy CS, Mayr T, Kürpig S, et al. ,
-
Journal of Cancer Research and Clinical Oncology ,
-
2021,
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Oncology
Background: In recent years, a variety of innovative therapeutics for castration-resistant prostate cancer have been developed, including novel anti-androgenic drugs, such as abiraterone or VPC-13566. Therapeutic monitoring of these pharmaceuticals is performed either by measuring PSA levels in serum or...
View PublicationChemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells
-
Panella S, Muoio F, Jossen V, et al. ,
-
Cells ,
-
2021,
-
10(2):466
- Cell Type: MSCs
- Assay: Cell Count Assay
- Research area: Stem Cell Research
Adipose tissue is an abundant source of stem cells. However, liposuction cannot yield cell quantities sufficient for direct applications in regenerative medicine. Therefore, the development of GMP-compliant ex vivo expansion protocols is required to ensure the production of a “cell...
View PublicationHuman Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status
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Muoio F, Panella S, Jossen V, et al. ,
-
J Funct Biomater ,
-
2021,
-
12(2):25
- Cell Type: MSCs
- Assay: Cell Count Assay
- Research area: Stem Cell Research
Human adipose stem cells (hASCs) are promising candidates for cell-based therapies, but they need to be efficiently expanded in vitro as they cannot be harvested in sufficient quantities. Recently, dynamic bioreactor systems operated with microcarriers achieved considerable high cell densities....
View PublicationMitochondrial augmentation of CD34 + cells from healthy donors and patients with mitochondrial DNA disorders confers functional benefit
-
Jacoby E, Ben Yakir-Blumkin M, Blumenfeld-Kan S, et al. ,
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NPJ Regen Med ,
-
2021,
-
6(1):58
- Cell Type: HSCs
- Assay: Cell Count Assay
- Research area: Cell Biology
Mitochondria are cellular organelles critical for numerous cellular processes and harboring their own circular mitochondrial DNA (mtDNA). Most mtDNA associated disorders (either deletions, mutations, or depletion) lead to multisystemic disease, often severe at a young age, with no disease-modifying therapies....
View PublicationOvercoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactors
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Borys BS, Dang T, So T, et al. ,
-
Stem Cell Research & Therapy ,
-
2021,
-
12(1):55
- Assay: Cell Count Assay
- Research area: Cell Biology
Background: Human induced pluripotent stem cells (hiPSCs) hold enormous promise in accelerating breakthroughs in understanding human development, drug screening, disease modeling, and cell and gene therapies. Their potential, however, has been bottlenecked in a mostly laboratory setting due to bioprocess...
View PublicationMicroRNAs and Regulation of Autophagy in Chondrocytes
-
Ramos YFM, Mobasheri A ,
-
Methods in Molecular Biology ,
-
2021,
-
2245:179-194
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Chondrocytes are the main cells responsible for the maintenance of cartilage homeostasis and integrity. During development, extracellular matrix (ECM) macromolecules are produced and deposited by chondrocyte precursors. Autophagy, a highly dynamic process aimed at degradation of dysfunctional or pathogenic proteins,...
View PublicationCombination therapy with Treg and mesenchymal stromal cells enhances potency and attenuation of inflammation after traumatic brain injury compared to monotherapy
-
Caplan HW, Prabhakara KS, Toledano Furman NE, et al. ,
-
Stem cells ,
-
2021,
-
39(3):358-370
- Cell Type: MSCs
- Assay: Cell Count Assay
- Research area: Cell-based Immunotherapy
The inflammatory response after traumatic brain injury (TBI) can lead to significant secondary brain injury and chronic inflammation within the central nervous system. Cell therapies, including mesenchymal stromal cells (MSC), have led to improvements in animal models of TBI and...
View PublicationSeverity of heterosubtypic influenza virus infection in ferrets is reduced by live attenuated influenza vaccine
-
Marriott AC, Gooch KE, Brown PJ, et al. ,
-
NPJ Vaccines ,
-
2021,
-
6(1):43
- Cell Type: Whole Blood
- Assay: Cell Count Assay
- Research area: Immunology
Live attenuated influenza vaccine (LAIV) is widely used to protect humans from seasonal influenza infection, particularly in children. In contrast to inactivated vaccines, the LAIV can induce both mucosal and cellular immune responses. Here we show that a single dose...
View PublicationThermal conditioning improves quality and speed of keratinocyte sheet production for burn wound treatment
-
Frese L, Darwiche SE, von Rechenberg B, et al. ,
-
Cytotherapy ,
-
2021,
-
S1465-3249(21)00031-1
- Cell Type: Epithelial Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Background aims: Cultured patient-specific keratinocyte sheets have been used clinically since the 1970s for the treatment of large severe burns. However, despite significant developments in recent years, successful and sustainable treatment is still a challenge. Reliable, high-quality grafts with faster...
View PublicationActivin A‐derived human embryonic stem cells show increased competence to differentiate into primordial germ cell‐like cells
-
Mishra S, Taelman J, Popovic M, et al. ,
-
Stem Cells ,
-
2021,
- Assay: Cell Count Assay
- Research area: Cell Biology
Protocols for specifying human primordial germ cell-like cells (hPGCLCs) from human embryonic stem cells (hESCs) remain hindered by differences between hESC lines, their derivation methods, and maintenance culture conditions. This poses significant challenges for establishing reproducible in vitro models of...
View PublicationCombination effect of nanoparticles on the acute pulmonary inflammogenic potential: additive effect and antagonistic effect
-
Lee S, Lee DK, Jeon S, et al. ,
-
Nanotoxicology ,
-
2021,
-
15(2):276-288
- Cell Type: Immune Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
The combination effect of co-exposed different types of nanomaterials is little known although humans are generally exposed to a mixture of nanomaterials from urban ultrafine particles or industrial nanomaterials. Herein, we evaluated the combined effect of nanoparticles (NPs) using three...
View PublicationAdipose tissue derived stromal vascular fraction as an adjuvant therapy in stroke rehabilitation: Case reports
-
Lee HB, Park SW, Kim IK, et al. ,
-
Medicine (Baltimore) ,
-
2020,
-
99(34):e21846
- Cell Type: MSCs
- Assay: Cell Count Assay
- Research area: Neurobiology
The intestinal gut microbiota is essential for maintaining host health. Concerns have been raised about the possible connection between antibiotic use, causing microbiota disturbances, and the increase in allergic and autoimmune diseases observed during the last decades. To elucidate the...
View PublicationJAK2 inhibition in JAK2V617F-bearing leukemia cells enriches CD34+ leukemic stem cells that are abolished by the telomerase inhibitor GRN163L
-
Dahlström J, Xia C, Xing X, et al. ,
-
Biochemical and Biophysical Research Communications ,
-
2020,
-
527(2):425-431
- Cell Type: Cancer Cells
- Assay: Cell Count Assay
- Research area: Oncology
The activating-mutation of JAK2V617F drives the development of myeloproliferative neoplasms (MPNs). Several JAK2 inhibitors such as ruxolitinib and gandotinib (LY2784544) currently in clinical trials and, provide improvements in MPNs including myelofibrosis. However, JAK2 inhibitors are non-curative and murine experiments show...
View PublicationInduction of Myogenic Differentiation Improves Chemosensitivity of Chemoresistant Cells in Soft-Tissue Sarcoma Cell Lines
-
Dawson LE, D'Agostino L, Hakim AA, et al. ,
-
Sarcoma ,
-
2020,
-
2020:8647981
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Oncology
Rhabdomyosarcoma (RMS) and rhabdoid tumors (RT) are rare soft-tissue malignancies with the highest incidence in infants, children, and adolescents. Advanced, recurrent, and/or metastatic RMS and RT exhibit poor response to treatment. One of the main mechanisms behind resistance to treatment...
View PublicationUse of Epigenetic Therapy Shortens Duration of Standard Chemotherapy for Ovarian Cancer with Minimal Toxicity to Normal Tissue
-
Chang L, Hunter K, Aikins J, et al. ,
-
Journal of Cancer Science and Clinical Therapeutics ,
-
2020,
-
100-114
- Cell Type: Epithelial Cells
- Assay: Cell Count Assay
- Research area: Oncology
Objective: (1) Determine if sequential administration of standard chemotherapy (paclitaxel and cisplatin, P/C) with epigenetic drugs effectively targets ovarian cancer and limits toxicity to normal cells. (2) Define whether epigenetic treatment can shorten the exposure to P/C. Methods: Normal cells—adipocyte-derived...
View PublicationClinical Value of lncRNA MEG3 in High-Grade Serous Ovarian Cancer
-
Buttarelli M, De Donato M, Raspaglio G, et al. ,
-
Cancers ,
-
2020,
-
12(4):966
- Cell Type: Tumor Models
- Assay: Cell Count Assay
- Research area: Oncology
Long non-coding RNAs (lncRNAs) are emerging as regulators in cancer development and progression, and aberrant lncRNA profiles have been reported in several cancers. Here, we evaluated the potential of using the maternally expressed gene 3 (MEG3) tissue level as a...
View PublicationRescuing ESAT-6 Specific CD4 T Cells From Terminal Differentiation Is Critical for Long-Term Control of Murine Mtb Infection
-
Clemmensen HS, Knudsen NPH, Billeskov R, et al. ,
-
Frontiers in Immunology ,
-
2020,
-
11:585359
- Cell Type: Immune Cells
- Assay: Cell Count Assay
- Research area: Protein Production
In most cases, Mycobacterium tuberculosis (Mtb) causes life-long chronic infections, which poses unique challenges for the immune system. Most of the current tuberculosis (TB) subunit vaccines incorporate immunodominant antigens and at this point, it is poorly understood how the CD4...
View PublicationAn Approach towards a GMP Compliant In-Vitro Expansion of Human Adipose Stem Cells for Autologous Therapies
-
Jossen V, Muoio F, Panella S, et al. ,
-
Bioengineering ,
-
2020,
-
7(3):77
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Stem Cell Research
Human Adipose Tissue Stem Cells (hASCs) are a valuable source of cells for clinical applications (e.g., treatment of acute myocardial infarction and inflammatory diseases), especially in the field of regenerative medicine. However, for autologous (patient-specific) and allogeneic (off-the-shelf) hASC-based therapies,...
View PublicationEngineering carotenoid production in mammalian cells for nutritionally enhanced cell-cultured foods
-
Stout AJ, Mirliani AB, Soule-Albridge EL, et al. ,
-
Metabolic Engineering ,
-
2020,
-
62:126-137
- Assay: Cell Count Assay
- Research area: Cultured Meat
Metabolic engineering of mammalian cells has to-date focused primarily on biopharmaceutical protein production or the manipulation of native metabolic processes towards therapeutic aims. However, significant potential exists for expanding these techniques to diverse applications by looking across the taxonomic tree...
View PublicationAdipose Tissue-Derived Stromal Cells Induce a Highly Trophic Environment While Reducing Maturation of Monocyte-Derived Dendritic Cells
-
Juhl M, Follin B, Gad M, et al. ,
-
Stem Cells Int ,
-
2020,
-
2020:8868909
- Cell Type: Whole Blood
- Assay: Cell Count Assay
- Research area: Immunology
Allogeneic cell-based therapies using adipose tissue-derived stromal cells (ASCs) offer an off-the-shelf alternative to autologous therapy. An underlying assumption is that ASC can modulate the immune response of the recipient. However, in vitro models are required to explore and identify...
View PublicationBacterial aggregate size determines phagocytosis efficiency of polymorphonuclear leukocytes
-
Alhede M, Lorenz M, Fritz BG, et al. ,
-
Medical Microbiology and Immunology ,
-
2020,
-
209(6):669-680
- Cell Type: Whole Blood
- Assay: Cell Count Assay
- Research area: Immunology
The ability of bacteria to aggregate and form biofilms impairs phagocytosis by polymorphonuclear leukocytes (PMNs). The aim of this study was to examine if the size of aggregates is critical for successful phagocytosis and how bacterial biofilms evade phagocytosis. We...
View PublicationShort-Term Amoxicillin-Induced Perturbation of the Gut Microbiota Promotes Acute Intestinal Immune Regulation in Brown Norway Rats
-
Graversen KB, Bahl MI, Larsen JM, et al. ,
-
Frontiers in Microbiology ,
-
2020,
-
11:496
- Cell Type: Immune Cells
- Assay: Cell Count Assay
- Research area: Immunology
The intestinal gut microbiota is essential for maintaining host health. Concerns have been raised about the possible connection between antibiotic use, causing microbiota disturbances, and the increase in allergic and autoimmune diseases observed during the last decades. To elucidate the...
View PublicationGeneration and characterization of genetically stable heterohybridomas producing foot-and-mouth disease virus-specific porcine monoclonal antibodies
-
Puckette MC, Martel E, Rutherford J, et al. ,
-
Journal of Immunological Methods ,
-
2020,
-
487:112873
- Cell Type: Immune Cells
- Assay: Cell Count Assay
- Research area: Immunology
This report covers the methodology for generation of stable heterohybridoma clones producing Foot-and-mouth disease virus (FMDV) reactive porcine monoclonal antibodies (mAbs). Swine received five inoculations of an inactivated O1 Manisa FMDV vaccine prior to the harvest of splenocytes. Due to...
View PublicationMcl-1 protects eosinophils from apoptosis and exacerbates allergic airway inflammation
-
Felton JM, Dorward DA, Cartwright JA, et al. ,
-
Thorax ,
-
2020,
-
75(7):600-605
- Cell Type: Immune Cells
- Assay: Cell Count Assay
- Research area: Immunology
Eosinophils are key effector cells in allergic diseases. Here we investigated Mcl-1 (an anti-apoptotic protein) in experimental allergic airway inflammation using transgenic overexpressing human Mcl-1 mice (hMcl-1) and reducing Mcl-1 by a cyclin-dependent kinase inhibitor. Overexpression of Mcl-1 exacerbated allergic...
View PublicationHuman cord blood‐derived regulatory T‐cell therapy modulates the central and peripheral immune response after traumatic brain injury
-
Caplan HW, Prabhakara KS, Kumar A, et al. ,
-
STEM CELLS Translational Medicine ,
-
2020,
-
9(8):903-916
- Cell Type: MSCs
- Assay: Cell Count Assay
- Research area: Cell-based Immunotherapy
Traumatic brain injury (TBI) causes a profound inflammatory response within the central nervous system and peripheral immune system, which contributes to secondary brain injury and further morbidity and mortality. Preclinical investigations have demonstrated that treatments that downregulate microglia activation and...
View PublicationA Staphylococcus pro-apoptotic peptide induces acute exacerbation of pulmonary fibrosis
-
D'Alessandro-Gabazza CN, Kobayashi T, Yasuma T, et al. ,
-
Nature Communications ,
-
2020,
-
11(1):1539
- Cell Type: Epithelial Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Idiopathic pulmonary fibrosis (IPF) is a chronic and fatal disease of unknown etiology; however, apoptosis of lung alveolar epithelial cells plays a role in disease progression. This intractable disease is associated with increased abundance of Staphylococcus and Streptococcus in the...
View PublicationOptimized serial expansion of human induced pluripotent stem cells using low‐density inoculation to generate clinically relevant quantities in vertical‐wheel bioreactors
-
Borys BS, So T, Colter J, et al. ,
-
STEM CELLS Translational Medicine ,
-
2020,
-
9(9):1036-1052
- Assay: Cell Count Assay
- Research area: Cell Biology
Human induced pluripotent stem cells (hiPSCs) have generated a great deal of attention owing to their capacity for self-renewal and differentiation into the three germ layers of the body. Their discovery has facilitated a new era in biomedicine for understanding...
View PublicationRegenerative Therapy for Liver Cirrhosis Based on Intrahepatic Arterial Infusion of Autologous Subcutaneous Adipose Tissue-Derived Regenerative (Stem) Cells: Protocol for a Confirmatory Multicenter Uncontrolled Clinical Trial
-
Sakai Y, Fukunishi S, Takamura M, et al. ,
-
JMIR Research Protocols ,
-
2020,
-
9(3):e17904
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Background: Liver cirrhosis results from chronic hepatitis, and is characterized by advanced fibrosis due to long-term hepatic inflammation. Cirrhosis ultimately leads to manifestations of jaundice, ascites, and encephalopathy, and increases the risk of hepatocellular carcinoma. Once cirrhosis is established, resulting...
View PublicationGeneration of myogenic progenitor cell-derived smooth muscle cells for sphincter regeneration
-
Thurner M, Deutsch M, Janke K, et al. ,
-
Stem Cell Research ,
-
2020,
-
11(1):233
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Background: Degeneration of smooth muscles in sphincters can cause debilitating diseases such as fecal incontinence. Skeletal muscle-derived cells have been effectively used in clinics for the regeneration of the skeletal muscle sphincters, such as the external anal or urinary sphincter....
View PublicationScale-up manufacturing of gelatin-based microcarriers for cell therapy.
-
Dosta P, Ferber S, Zhang Y, et al. ,
-
Journal of Biomedical Materials Research ,
-
2020,
-
108(7):2937-2949
- Cell Type: MSCs
- Assay: Cell Count Assay
- Research area: Cell Biology
Microcarriers, including crosslinked porous gelatin beads (Cultispher G) are widely used as cell carriers for cell therapy applications. Microcarriers can support a range of adherent cell types in stirred tank bioreactor culture, which is scalable up to several thousands of...
View PublicationBioreactor Parameters for Microcarrier-Based Human MSC Expansion under Xeno-Free Conditions in a Vertical-Wheel System
-
Lembong J, Kirian R, Takacs JD, et al. ,
-
Bioengineering ,
-
2020,
-
7(3):73
- Cell Type: MSCs
- Assay: Cell Count Assay
- Research area: Cell Biology
Human mesenchymal stem/stromal cells (hMSCs) have been investigated and proven to be a well-tolerated, safe therapy for a variety of indications, as shown by over 900 registered hMSC-based clinical trials. To meet the commercial demand for clinical manufacturing of hMSCs,...
View PublicationSerine administration as a novel prophylactic approach to reduce the severity of acute pancreatitis during diabetes in mice
-
Chen R, Hornemann T, Štefanić S, et al. ,
-
Diabetologia ,
-
2020,
-
63(9):1885-1899
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Aims/hypothesis: Compared with the general population, individuals with diabetes have a higher risk of developing severe acute pancreatitis, a highly debilitating and potentially lethal inflammation of the exocrine pancreas. In this study, we investigated whether 1-deoxysphingolipids, atypical lipids that increase...
View PublicationSelf‐healing injectable gelatin hydrogels for localized therapeutic cell delivery
-
Sisso AM, Boit MO, DeForest CA ,
-
Journal of Biomedical Research Part A ,
-
2020,
-
108(5):1112-1121
- Assay: Cell Count Assay
- Research area: Stem Cell Research
Self-healing injectable hydrogel biomaterials uniquely enable precise therapeutic deposition and deployment at specific bodily locations through versatile and minimally invasive processes that can preserve cargo integrity and cell viability. Despite the distinct advantages that injectable hydrogels offer in tissue engineering...
View PublicationImpedimetric melanoma invasion assay device using a simple paper membrane and stencil-printed electrode on PMMA substrate
-
Pupinyo N, Heiskanen A, Chailapakul O, et al. ,
-
Sensing and Bio-Sensing Research ,
-
2020,
-
29:100354
- Cell Type: Cancer Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
The transwell assay is currently the most popular approach to studying cellular invasion due to its ease of use and readout, and the possibility for quantitative measurements. However, it only allows end-point measurements without the possibility for real-time tracking of...
View PublicationInspired by the human placenta: a novel 3D bioprinted membrane system to create barrier models
-
Kreuder AE, Bolaños-Rosales A, Palmer C, et al. ,
-
Scientific Reports ,
-
2020,
-
10(1):15606
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Barrier organ models need a scaffold structure to create a two compartment culture. Technical filter membranes used most often as scaffolds may impact cell behaviour and present a barrier themselves, ultimately limiting transferability of test results. In this work we...
View PublicationProduction of a novel heterodimeric two-chain insulin-Fc fusion protein
-
Faust C, Ochs C, Korn M, et al. ,
-
Protein Engineering Design & Selection ,
-
2020,
-
33:gzaa026
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Insulin is a peptide hormone produced by the pancreas. The physiological role of insulin is the regulation of glucose metabolism. Under certain pathological conditions the insulin levels can be reduced leading to the metabolic disorder diabetes mellitus (DM). For type...
View PublicationAging-regulated anti-apoptotic long non-coding RNA Sarrah augments recovery from acute myocardial infarction
-
Trembinski DJ, Bink DI, Theodorou K, et al. ,
-
Nature Communications ,
-
2020,
-
11(1):2039
- Cell Type: Epithelial Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Long non-coding RNAs (lncRNAs) contribute to cardiac (patho)physiology. Aging is the major risk factor for cardiovascular disease with cardiomyocyte apoptosis as one underlying cause. Here, we report the identification of the aging-regulated lncRNA Sarrah (ENSMUST00000140003) that is anti-apoptotic in cardiomyocytes....
View PublicationAccelerated neuronal and synaptic maturation by BrainPhys medium increases Aβ secretion and alters Aβ peptide ratios from iPSC-derived cortical neurons
-
Satir TM, Nazir FH, Vizlin-Hodzic D, et al. ,
-
Scientific Reports ,
-
2020,
-
10(1):601
- Assay: Cell Count Assay
- Research area: Stem Cell Research
One of the neuropathological hallmarks of Alzheimer’s disease (AD) is cerebral deposition of amyloid plaques composed of amyloid β (Aβ) peptides and the cerebrospinal fluid concentrations of those peptides are used as a biomarker for AD. Mature induced pluripotent stem...
View PublicationIn vivo Environment Swiftly Restricts Human Pancreatic Progenitors Toward Mono-Hormonal Identity via a HNF1A/HNF4A Mechanism
-
Legøy TA, Mathisen AF, Salim Z, et al. ,
-
Frontiers in Cell and Developmental Biology ,
-
2020,
-
0,409027778
- Assay: Cell Count Assay
- Research area: Stem Cell Research
Generating insulin-producing β-cells from human induced pluripotent stem cells is a promising cell replacement therapy for improving or curing insulin-dependent diabetes. The transplantation of end-stages differentiating cells into living hosts was demonstrated to improve β-cell maturation. Nevertheless, the cellular and...
View PublicationFormation of Mature Nephrons by Implantation of Human Pluripotent Stem Cell-Derived Progenitors into Mice
-
Bantounas I, Silajdžić E, Woolf AS, et al. ,
-
Methods in Molecular Biology ,
-
2020,
-
2067:309-322
- Assay: Cell Count Assay
- Research area: Stem Cell Research
In the future, stem cell-based technologies may be harnessed to replace conventional dialysis and transplantation in patients with diabetic nephropathy. Recently, there has been considerable effort to improve methods for the differentiation of human pluripotent stem cells (hPSCs) into kidney...
View PublicationKnockout of sialidase and pro-apoptotic genes in Chinese hamster ovary cells enables the production of recombinant human erythropoietin in fed-batch cultures
-
Ha TK, Hansen AH, Kildegaard HF, Lee GM ,
-
Metabolic Engineering ,
-
2020,
-
57:182-192
- Cell Type: CHO Cells
- Assay: Cell Count Assay
- Research area: Protein Production
Sialic acid, a terminal monosaccharide present in N-glycans, plays an important role in determining both the in vivo half-life and the therapeutic efficacy of recombinant glycoproteins. Low sialylation levels of recombinant human erythropoietin (rhEPO) in recombinant Chinese hamster ovary (rCHO)...
View PublicationModel uncertainty-based evaluation of process strategies during scale-up of biopharmaceutical processes
-
Möller Tanja, Hernández Rodríguez, Jan Müller, et al. ,
-
Computers and Chemical Engineering ,
-
2020,
-
134:106693
- Cell Type: CHO Cells
- Assay: Cell Count Assay
- Research area: Protein Production
Reliable scale-up of biopharmaceutical production processes is key in Quality by Design. In this study, a model-based workflow is described to evaluate the bioprocess dynamics during process transfer and scale-up computationally. First, a mathematical model describes the bioprocess dynamics of...
View PublicationMultiplex secretome engineering enhances recombinant protein production and purity
-
Stefan Kol, Daniel Ley, Tune Wulff, et al. ,
-
Nature Communications ,
-
2020,
-
volume 11, 1908 (2020)
- Cell Type: CHO Cells
- Assay: Cell Count Assay
- Research area: Protein Production
Host cell proteins (HCPs) are process-related impurities generated during biotherapeutic protein production. HCPs can be problematic if they pose a significant metabolic demand, degrade product quality, or contaminate the final product. Here, we present an effort to create a “clean”...
View PublicationEvolution of ASC Immunophenotypical Subsets During Expansion In Vitro
-
Peng Q, Alipour H, Porsborg S, et al. ,
-
International Journal of Molecular Sciences ,
-
2020,
-
21(4). pii: E1408
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Stem Cell Research
Adipose-derived stromal/stem cells (ASCs) are currently being considered for clinical use for a number of indications. In order to develop standardized clinical protocols, it is paramount to have a full characterization of the stem cell preparations. The surface marker expression...
View Publication39°C-in vitro culture facilitates hepatic functions of hepatocyte-like cells derived from human embryonic stem cells
-
Satoshi Imamura, Koki Yoshimoto, Shiho Terada, View ORCID ProfileKen-ichiro Kamei ,
-
bioRxiv ,
-
2020,
- Cell Type: Hepatocytes
- Assay: Cell Count Assay
- Research area: Stem Cell Research
Hepatocyte-like cells derived from human pluripotent stem cells (hPSC-HLCs) offer an alternative to primary hepatocytes commonly used for drug screenings and toxicological tests. Although tremendous efforts have been made to facilitate hepatic functions of hPSC-HLCs using growth factors and chemicals,...
View PublicationEncapsulation boosts islet-cell signature in differentiating human induced pluripotent stem cells via integrin signalling
-
Legøy TA, Vethe H, Abadpour S, et al. ,
-
Scientific Reports ,
-
2020,
-
10(1):414
- Assay: Cell Count Assay
- Research area: Cell-based Immunotherapy Therapy
Cell replacement therapies hold great therapeutic potential. Nevertheless, our knowledge of the mechanisms governing the developmental processes is limited, impeding the quality of differentiation protocols. Generating insulin-expressing cells in vitro is no exception, with the guided series of differentiation events...
View PublicationSubvisible Particulate Contamination in Cell Therapy Products-Can We Distinguish?
-
Vollrath I, Mathaes R, Sediq AS, et al. ,
-
Journal of Pharmaceutical Sciences ,
-
2020,
-
109(1):216-219
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Cell-based Immunotherapy Therapy
Cell therapy products represent an exciting new class of medicinal products, which must be parenterally administered. Thus, compliance with parenteral preparation guidelines is required. One requirement for parenteral products is the characterization of particle contaminations. As cell-based products are turbid...
View PublicationB lymphocytes directly contribute to tissue fibrosis in patients with IgG4-related disease
-
Della-Torre E, Rigamonti E, Perugino C, et al. ,
-
Journal of Allergy and Clinical Immunology ,
-
2020,
-
145(3):968-981
- Cell Type: PBMCs
- Assay: Cell Count Assay
- Research area: Immunology
Background: IgG4-related disease (IgG4-RD) is a fibroinflammatory condition marked by rapid clinical improvement after selective depletion of B lymphocytes with rituximab. This feature suggests that B cells might participate in fibrogenesis and wound healing. Objective: In the present work we...
View PublicationMass cytometry reveals cellular fingerprint associated with IgE+ peanut tolerance and allergy in early life.
-
Neeland MR, Andorf S, Manohar M, et al. ,
-
Nature Communications ,
-
2020,
-
11(1):1091
- Cell Type: PBMCs
- Assay: Cell Count Assay
- Research area: Immunology
IgE-mediated peanut allergic is common, often serious, and usually lifelong. Not all individuals who produce peanut-specific IgE will react upon consumption of peanut and can eat the food without adverse reactions, known as sensitized tolerance. Here, we employ high-dimensional mass...
View PublicationDistinct microbial and immune niches of the human colon
-
James KR, Gomes T, Elmentaite R, et al. ,
-
Nature Immunology ,
-
2020,
-
21(3):343-353
- Cell Type: T Cells
- Assay: Cell Count Assay
- Research area: Immunology
Gastrointestinal microbiota and immune cells interact closely and display regional specificity; however, little is known about how these communities differ with location. Here, we simultaneously assess microbiota and single immune cells across the healthy, adult human colon, with paired characterization...
View PublicationBevacizumab suppresses the growth of established non-small-cell lung cancer brain metastases in a hematogenous brain metastasis model
-
Masuda C, Sugimoto M, Wakita D, et al. ,
-
Clinical and Experimental Metastasis ,
-
2020,
-
37(1):199-207
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Oncology
Brain metastases are common in patients with non-small-cell lung cancer (NSCLC). The efficacy of bevacizumab, an anti-vascular endothelial growth factor (VEGF) humanized antibody, has been demonstrated in patients with nonsquamous NSCLC. We established a transplantable NSCLC cell line (Nluc-H1915) that...
View PublicationAwakening dormant glycosyltransferases in CHO cells with CRISPRa
-
Karottki KJC, Hefzi H, Xiong K, et al. ,
-
Biotechnol Bioeng ,
-
2020,
-
117(2):593-598
- Cell Type: CHO Cells
- Assay: Cell Count Assay
- Research area: Protein Production
Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells. Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription...
View PublicationGenome-scale reconstructions of the mammalian secretory pathway predict metabolic costs and limitations of protein secretion
-
Gutierrez JM, Feizi A, Li S, et al. ,
-
Nature Communications ,
-
2020,
-
11(1):68
- Cell Type: CHO Cells
- Assay: Cell Count Assay
- Research area: Protein Production
In mammalian cells, >25% of synthesized proteins are exported through the secretory pathway. The pathway complexity, however, obfuscates its impact on the secretion of different proteins. Unraveling its impact on diverse proteins is particularly important for biopharmaceutical production. Here we...
View PublicationAkt1 signalling supports acinar proliferation and limits acinar‐to‐ductal metaplasia formation upon induction of acute pancreatitis
-
Chen R, Malagola E, Dietrich M, et al. ,
-
Journal of Pathology ,
-
2020,
-
250(1):42-54
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Molecular signalling mediated by the phosphatidylinositol-3-kinase (PI3K)-Akt axis is a key regulator of cellular functions. Importantly, alteration of the PI3K-Akt signalling underlies the development of different human diseases, thus prompting the investigation of the pathway as a molecular target for...
View PublicationFasting‐ and ghrelin‐induced food intake is regulated by NAMPT in the hypothalamus
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de Guia RM, Hassing AS, Skov LJ, et al. ,
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Acta Physiologica ,
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2020,
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228(4):e13437
- Cell Type: Neural Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Aim: Neurons in the arcuate nucleus of the hypothalamus are involved in regulation of food intake and energy expenditure, and dysregulation of signalling in these neurons promotes development of obesity. The role of the rate-limiting enzyme in the NAD+ salvage...
View PublicationLarge‐scale expansion of feeder‐free mouse embryonic stem cells serially passaged in stirred suspension bioreactors at low inoculation densities directly from cryopreservation
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Borys BS, So T, Roberts EL, et al. ,
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Biotechnology and Bioengineering ,
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2020,
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117(5):1316-1328
- Assay: Cell Count Assay
- Research area: Cell-based Immunotherapy Therapy
Embryonic stem cells (ESCs) have almost unlimited proliferation capacity in vitro and can retain the ability to contribute to all cell lineages, making them an ideal platform material for cell-based therapies. ESCs are traditionally cultured in static flasks on a...
View PublicationPulmonary toxicity of Fe2O3, ZnFe2O4, NiFe2O4 and NiZnFe4O8 nanomaterials: Inflammation and DNA strand breaks.
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Hadrup N, Saber AT, Kyjovska ZO, et al. ,
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Environmental Toxicology and Pharmacology ,
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2020,
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74:103303
- Cell Type: Cancer Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Exposure to metal oxide nanomaterials potentially occurs at the workplace. We investigated the toxicity of two Fe-oxides: Fe2O3 nanoparticles and nanorods; and three MFe2O4 spinels: NiZnFe4O8, ZnFe2O4, and NiFe2O4 nanoparticles. Mice were dosed 14, 43 or 128 μg by intratracheal...
View PublicationNuclear and Cytoplasmatic Quantification of Unconjugated, Label-Free Locked Nucleic Acid Oligonucleotides
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Pendergraff H, Schmidt S, Vikeså J, et al. ,
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Nucleic Acid Therapeutics ,
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2020,
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30(1):4-13
- Cell Type: Cancer Cells
- Assay: Cell Count Assay
- Research area: Cell Biology
Methods for the quantification of antisense oligonucleotides (AONs) provide insightful information on biodistribution and intracellular trafficking. However, the established methods have not provided information on the absolute number of molecules in subcellular compartments or about how many AONs are needed...
View PublicationMulti-omics characterization of a diet-induced obese model of non-alcoholic steatohepatitis
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Ægidius HM, Veidal SS, Feigh M, et al. ,
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Scientific Reports ,
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2020,
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10(1):1148
- Cell Type: Hepatocytes
- Assay: Cell Count Assay
- Research area: Cell Biology
To improve the understanding of the complex biological processes underlying the development of non-alcoholic steatohepatitis (NASH), a multi-omics approach combining bulk RNA-sequencing based transcriptomics, quantitative proteomics and single-cell RNA-sequencing was used to characterize tissue biopsies from histologically validated diet-induced obese...
View PublicationIGF1-mediated human embryonic stem cell self-renewal recapitulates the embryonic niche
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Wamaitha SE, Grybel KJ, Alanis-Lobato G, et al. ,
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Nature Communications ,
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2020,
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11(1):764
- Assay: Cell Count Assay
- Research area: Cell Biology
Our understanding of the signalling pathways regulating early human development is limited, despite their fundamental biological importance. Here, we mine transcriptomics datasets to investigate signalling in the human embryo and identify expression for the insulin and insulin growth factor 1...
View PublicationVolatile organic compound (VOC) emissions of CHO and T cells correlate to their expansion in bioreactors
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McCartney MM, Yamaguchi MS, Bowles PA, et al. ,
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Journal of Breath Research ,
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2019,
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14(1):016002
- Cell Type: CHO Cells
- Assay: Cell Count Assay
- Research area: Protein Production
Volatile organic compound (VOC) emissions were measured from Chinese Hamster Ovary (CHO) cell and T cell bioreactor gas exhaust lines with the goal of non-invasively metabolically profiling the expansion process. Measurements of cellular ‘breath’ were made directly from the gas...
View PublicationA generalized HIV vaccine design strategy for priming of broadly neutralizing antibody responses
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Steichen JM, Lin YC, Havenar-Daughton C, et al. ,
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Science ,
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2019,
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366(6470)
- Cell Type: Mammalian Cells
- Assay: Cell Count Assay
- Research area: Protein Production
Vaccine induction of broadly neutralizing antibodies (bnAbs) to HIV remains a major challenge. Germline-targeting immunogens hold promise for initiating the induction of certain bnAb classes; yet for most bnAbs, a strong dependence on antibody heavy chain complementarity-determining region 3 (HCDR3)...
View Publication