Cell Cycle Assay
Analyze Fixed or Live Cells in Two Simple Steps Using the Cell Cycle Assay
- Fast and easy measurement of cell cycle phases
- Single-step acquisition, analysis, and data presentation
- Standardized results – even between different users
- No RNase treatment required
- No calibration required
- Clear data presentation and management
- Data export in FCS/ACS formats
Overview of the Cell Cycle Assay
Cell cycle and cell division represent the most fundamental and important processes in eukaryotic cells. With the Cell Cycle Assay, DAPI fluorescence intensities, representative of individual cell cycle phases, are quantified and displayed in a user-friendly interface.
The 2-Step Cell Cycle Assay (upper flow) facilitates detaching, permeabilization, de-clumping and homogenous staining of the cell population in two simple steps without trypsinization, washing or centrifugation. The Cell Cycle of Fixed Cells Assay (lower flow) enables cell storage for several weeks. Thus, after ethanol fixation, the cells can be retained in cold storage for a much longer period before sample analysis.
With the NucleoCounter® NC-3000™ cell cycle analysis, simply prepare your sample by lysis or fixation, then add staining buffers, load the sample, and press run. After the one-minute data acquisition and analysis is complete, the cell cycle profile data and percentage or number of events in G0/G1, S, G2 or sub-G1 phases displays in NucleoView™ software’s Plot Manager feature.
With the FlexiCyte™ software package, the NC-3000™ can even be used for studying cell proliferation using BrdU- or EdU-incorporation. When detected by fluorescently labeled antibodies or Click Chemistry, these allow for advanced studies of cell proliferation.
Assay Principle
The Cell Cycle Assay uses the nuclear stain DAPI to measure DNA content. DAPI binds specifically to double-stranded DNA. Consequently, there is no requirement to remove RNA prior to DNA content measurements. This is a prerequisite for other dyes commonly used for measurement of cell cycle phases, such as propidium iodide (PI).
Using fluorescence microscopy and image analysis, DNA content quantification and measurements of cell cycle phases are automated.
Sample preparations can be loaded into either of two slide types:
Samples are analyzed using the NucleoCounter® NC-3000™ advanced image cytometer where cellular fluorescence is quantified into histograms displaying the DNA content quantification. Markers in the displayed histograms, M1 – M4, can be used to identify cells in different cell cycle stages: sub-G1-phase, G0/G1-phase, S-phase and G2/M-phase, respectively.
Results Presented in Plot Manager
The histogram plots depict U2OS cells grown in absence (upper row) or presence (lower row) of etoposide. DNA content was measured using the 2-Step Cell Cycle Assay in the NC-3000™. Scatter plots, histograms and tables were obtained using NucleoView™ NC-3000™ software.
Markers in the histograms were used to demarcate cells in the different cell cycle phases. The colored histogram is a merge of untreated (blue line) and etoposide treated (red line) samples. G0/G1 indicated by the M1 marker, S phase indicated by M2, and the G2/M phase cells indicated by M3.
Publications
Acoustophoresis Enables the Label-Free Separation of Functionally Different Subsets of Cultured Bone Marrow Stromal Cells
-
Olm F, Lim HC, Schallmoser K, et al. ,
-
Cytometry A ,
-
2021,
-
99(5):476-487
- Instrument: NucleoCounter® NC-250™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay, Cell Cycle Assay
- Research area: Stem Cell Therapy
Objective: As the development and progression of colorectal cancer (CRC) are known to be affected by the immune system, cell subsets such as T cells, natural killer (NK) cells, and natural killer T (NKT) cells are considered interesting targets for...
View PublicationPhosphorylation of levan by microwave-assisted synthesis enhanced anticancer ability
-
Huang TY, Huang MY, Tsai CK, Su WT ,
-
Journal of Bioscience and Bioengineering ,
-
2021,
-
131(1):98-106
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, Cell Vitality Assay
- Research area: Oncology
The macrophage-associated molecule CD163 has been reported as a prognostic biomarker in different cancer types, but its role in colorectal cancer (CRC) is unclear. We studied CD163 in the tumor microenvironment and circulation of patients with CRC in relation to...
View PublicationIdentification of a transient state during the acquisition of temozolomide resistance in glioblastoma
-
Rabé M, Dumont S, Álvarez-Arenas A, et al. ,
-
Cell Death Dis ,
-
2020,
-
11(1):19
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Drug resistance limits the therapeutic efficacy in cancers and leads to tumor recurrence through ill-defined mechanisms. Glioblastoma (GBM) are the deadliest brain tumors in adults. GBM, at diagnosis or after treatment, are resistant to temozolomide (TMZ), the standard chemotherapy. To...
View PublicationAlpha-lipoic acid alters the antitumor effect of bortezomib in melanoma cells in vitro
-
Takács A, Lajkó E, Láng O, et al. ,
-
Scientific Reports ,
-
2020,
-
10(1):14287
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
The circadian clock is a critical regulator of immune function. We recently highlighted a role for the circadian clock in a mouse model of pulmonary inflammation. The epithelial clock protein Bmal1 was required to regulate neutrophil recruitment in response to...
View PublicationInhibition of PARP Sensitizes Chondrosarcoma Cell Lines to Chemo- and Radiotherapy Irrespective of the IDH1 or IDH2 Mutation Status
-
Venneker S, Kruisselbrink AB, Briaire-de Bruijn IH, et al. ,
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Cancers ,
-
2019,
-
11(12):1918
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Pulmonary airway epithelial cells (AECs) form a critical interface between host and environment. We investigated the role of the circadian clock using mice bearing targeted deletion of the circadian gene brain and muscle ARNT-like 1 (Bmal1) in AECs. Pulmonary neutrophil...
View PublicationA screening-based approach identifies cell cycle regulators AURKA, CHK1 and PLK1 as targetable regulators of chondrosarcoma cell survival
-
de Jong Y, Bennani F, van Oosterwijk JG, et al. ,
-
J Bone Oncol ,
-
2019,
-
19:100268
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Culture-expanded mesenchymal stromal cells (MSCs) are promising candidates for clinical cell-based therapies. MSC products are heterogeneous and we therefore investigated whether acoustophoresis, an ultrasound-based separation technology, could be used for the label-free enrichment of functionally different MSC populations. Acoustophoresis uses...
View PublicationCHO cell cultures in shake flasks and bioreactors present different host cell protein profiles in the supernatant
-
Goey CH, Bell D, Kontoravdi C ,
-
Biochemical Engineering Journal ,
-
2019,
-
144:185–192
- Instrument: NucleoCounter® NC-250™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, Cell Vitality Assay
- Research area: Cell Biology
Purpose: Pseudomyogenic hemangioendothelioma (PHE) is an extremely rare locally aggressive neoplasm with endothelial differentiation, which often presents with multiple lesions. These tumors have characteristic SERPINE1-FOSB fusions. We report a 17 years old patient with advanced unresectable PHE with a durable...
View PublicationTelatinib Is an Effective Targeted Therapy for Pseudomyogenic Hemangioendothelioma
-
van IJzendoorn DGP, Sleijfer S, Gelderblom H, et al. ,
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Clin Cancer Res ,
-
2018,
-
24(11):2678-2687
- Instrument: NucleoCounter® NC-250™
- Cell Type: MSCs
- Assay: Cell Cycle Assay, Cell Vitality Assay
- Research area: Oncology
The addition of nutrients and accumulation of metabolites in a fed-batch culture of Chinese hamster ovary (CHO) cells leads to an increase in extracellular osmolality in late stage culture. Herein, we explore the effect of osmolality on CHO cell growth,...
View PublicationMechanistic characterization of a copper containing thiosemicarbazone with potent antitumor activity
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Karlsson H, Fryknäs M, Strese S, et al. ,
-
Oncotarget ,
-
2017,
-
8(18):30217-30234
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
The circadian clock is an intrinsic oscillator that imparts 24 h rhythms on immunity. This clock drives rhythmic repression of inflammatory arthritis during the night in mice, but mechanisms underlying this effect are not clear. Here we show that the...
View PublicationAnalysis of global DNA methylation changes in primary human fibroblasts in the early phase following X-ray irradiation
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Maierhofer A, Flunkert J, Dittrich M, et al. ,
-
PLoS One ,
-
2017,
-
12(5):e0177442
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Epigenetic alterations may contribute to the generation of cancer cells in a multi-step process of tumorigenesis following irradiation of normal body cells. Primary human fibroblasts with intact cell cycle checkpoints were used as a model to test whether X-ray irradiation...
View PublicationCascading effect in bioprocessing – The impact of mild hypothermia on CHO cell behaviour and host cell protein composition
-
Goey CH, Tsang JMH, Bell D, Kontoravdi C ,
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Biotechnol Bioeng ,
-
2017,
-
114(12):2771-2781
- Instrument: NucleoCounter® NC-250™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, Cell Vitality Assay
- Research area: Protein Production
Scope: Prenylated chalcones and flavonoids from hop (Humulus lupulus L.), such as 6-prenylnaringenin (6-PN) and 8-prenylnaringenin (8-PN), are investigated for their health beneficial and anticancer activities. We, thus, compare the oral bioavailability and safety of 6-PN and 8-PN in healthy...
View PublicationDiscovery of novel [1,2,4]triazolo[4,3-a]quinoxaline aminophenyl derivatives as BET inhibitors for cancer treatment
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Ali I, Lee J, Go A, et al. ,
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Bioorganic & Medicinal Chemistry Letters ,
-
2017,
-
27(20):4606-4613
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Bromodomain and extra-terminal (BET) proteins, a class of epigenetic reader domains has emerged as a promising new target class for small molecule drug discovery for the treatment of cancer, inflammatory, and autoimmune diseases. Starting from in silico screening campaign, herein...
View PublicationIdentification of Mitotic Blockers by Phenotypic Screening of a Clinical Compound Library
-
Son YH, CH Park, SU Choi ,
-
Bulletin of the Korean Chemical Society ,
-
2016,
-
37(12) 1942–1946
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Small Molecule Drugs
Metastasis is the major cause of death from lung cancer. Quercetin, a widely distributed bioflavonoid, is well known to induce growth inhibition in a variety of human cancer cells, but how it affects lung cancer cell invasion and metastasis is...
View PublicationNovel c-Met inhibitor suppresses the growth of c-Met-addicted gastric cancer cells.
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Park CH, Cho SY, Ha JD, et al. ,
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BMC Cancer ,
-
2016,
-
16(1):35
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Minor modifications to ceritinib enhance anti-tumor activity in EML4-ALK positive cancer.
-
Kang CH, Kim EY, Kim HR, et al. ,
-
Cancer Lett. ,
-
2016,
-
374(2):272-8
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
The stepwise evolution of the exome during acquisition of docetaxel resistance in breast cancer cells
-
Hansen SN, Ehlers NS, Zhu S, et al. ,
-
BMC Genomics ,
-
2016,
-
17(1):442
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Synthesis and Biological Evaluation of Tetrapeptide Ketones as Reversible 20S Proteasome Inhibitors
-
Latif M, MEL, Lee K, Gildon ,
-
Korean Chemical Society ,
-
2014,
-
35(12), 3571–3575
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Inhibition of brain tumor cell proliferation by alternating electric fields
-
Jeong H, Sung J, Oh SI, et al. ,
-
Applied Physics Letters ,
-
2014,
-
105(20), 203703
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Development of potent ALK inhibitor and its molecular inhibitory mechanism against NSCLC harboring EML4-ALK proteins.
-
Kang CH, Yun JI, Lee K, et al. ,
-
Biochem Biophys Res Commun. ,
-
2015,
-
464(3):762-7
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Biological evaluation of tanshindiols as EZH2 histone methyltransferase inhibitors.
-
Woo J, Kim HY, Byun BJ, et al. ,
-
Bioorg Med Chem Lett. ,
-
2014,
-
24(11):2486-92
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
A major challenge in downstream purification of monoclonal antibodies (mAb) is the removal of host cell proteins (HCPs). Previous studies have shown that cell culture conditions significantly impact the HCP content at harvest. However, it is currently unclear how process...
View PublicationNovel human-derived EML4-ALK fusion cell lines identify ribonucleotide reductase RRM2 as a target of activated ALK in NSCLC
-
Bokhari AA, Lai WY, Le AT, et al. ,
-
Lung Cancer ,
-
2022,
-
171:103-114
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Introduction Echinoderm microtubule-associated protein-like 4 (EML4)-Anaplastic Lymphoma Kinase (ALK) rearrangements occur in 3% to 7% of lung adenocarcinomas and are targets for treatment with tyrosine kinase inhibitors (TKIs). Here we have developed three novel EML4-ALK-positive patient–derived Non-Small-Cell-Lung-Cancer (NSCLC) cancer cell...
View PublicationResveratrol butyrate esters inhibit lipid biosynthesis in 3T3-L1 cells by AMP-activated protein kinase phosphorylation
-
Shih MK, Hsieh SL, Huang YW, et al. ,
-
Journal of Food Science and Technology ,
-
2022,
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Epithelial Cells
- Assay: Cell Cycle Assay, Cell Vitality Assay
- Research area: Cell Biology
Resveratrol butyrate esters (RBEs), which are novel resveratrol-synthesized derivatives, exhibit increased biological activity. This study elucidated the effect of RBEs on fat metabolism and their anti-obesity characteristics. Their molecular mechanism was investigated in the 3T3-L1 murine preadipocyte cells and adipocytes....
View PublicationLong Noncoding RNA LOC550643 Acts as an Oncogene in the Growth Regulation of Colorectal Cancer Cells
-
Wu HF, Lu TJ, Lo YH, et al. ,
-
Cells ,
-
2022,
-
11(7):1065
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Oncology
Long noncoding RNAs play a key role in the progression of colorectal cancer (CRC). However, the role and mechanism of LOC550643 in CRC cell growth and metastasis remain largely unknown. In this study, we assessed the clinical impacts of LOC550643...
View PublicationVariations in the Anticancer Activity of Free and Bound Phenolics of Finger Millet (Eleusine coracana (L) Gaertn; Variety KMR-301) Seeds
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Kuruburu MG, Bovilla VR, Naaz R, et al. ,
-
Phytomedicine Plus ,
-
2022,
-
2(2):100276
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, DNA Fragmentation Assay
- Research area: Oncology
Background Finger millet (FM, Eleusine coracana (L) Gaertn; also known as “ragi” in Kannada language) is one of the widely used millets especially in south India and parts of Africa. FM is consumed in various forms that include porridge, mudde...
View PublicationPhytochemical Profile and Biological Activity of the Ethanolic Extract from the Aerial Part of Crocus alatavicus Regel & Semen Growing Wildly in Southern Kazakhstan
-
Allambergenova Z, Kasela M, Adamczuk G, et al. ,
-
Molecules ,
-
2022,
-
27(11):3468
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay, Cell Vitality Assay
- Research area: Oncology
The composition of the ethanolic extract from the aerial parts of Crocus alatavicus Regel & Semen from southern Kazakhstan spontaneous flora was analyzed together with the determination of its antibacterial, antifungal, antiviral and anticancer activity. The phytochemical profile analysis by...
View PublicationChemosensitization of U-87 MG Glioblastoma Cells by Neobavaisoflavone towards Doxorubicin and Etoposide
-
Maszczyk M, Banach K, Karkoszka M, et al. ,
-
Int J Mol Sci ,
-
2022,
-
23(10):5621
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, Mitochondrial Potential Assay
- Research area: Oncology
Glioblastoma (GB) is the most common type of glioma, which is distinguished by high mortality. Due to the rapid progression of the tumor and drug resistance, the treatment is often ineffective. The development of novel therapies in a big part...
View PublicationDesign, synthesis, and biological activities of 3-((4,6-diphenylpyrimidin-2-ylamino)methylene)-2,3-dihydrochromen-4-ones
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Shin SY, Jung E, Yeo H, et al. ,
-
Bioorganic Chemistry ,
-
2022,
-
105634
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, DNA Fragmentation Assay
- Research area: Oncology
Novel (Z)-3-((4,6-diphenylpyrimidin-2-ylamino)methylene)-2,3-dihydrochromen-4-one derivatives were designed and synthesized to find chemotherapeutic agents. Derivative 9 was selected based on its clonogenicity against cancer cells and synthetic yield for further biological experiments. It showed decreases in aurora kinase A, B, and C phosphorylation...
View PublicationThe Anticancer Potential of Doxycycline and Minocycline-A Comparative Study on Amelanotic Melanoma Cell Lines
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Rok J, Rzepka Z, Kowalska J, et al. ,
-
Int J Mol Sci ,
-
2022,
-
23(2):831
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, Cell Vitality Assay, Mitochondrial Potential Assay
- Research area: Oncology
Malignant melanoma is still a serious medical problem. Relatively high mortality, a still-growing number of newly diagnosed cases, and insufficiently effective methods of therapy necessitate melanoma research. Tetracyclines are compounds with pleiotropic pharmacological properties. Previously published studies on melanotic melanoma...
View PublicationPotential Anticancer Agents against Melanoma Cells Based on an As-Synthesized Thiosemicarbazide Derivative
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Kozyra P, Korga-Plewko A, Karczmarzyk Z, et al. ,
-
Biomolecules ,
-
2022,
-
12(2):151
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, Cell Vitality Assay
- Research area: Oncology
In this paper, thiosemicarbazide derivatives were synthesized as potential anticancer agents. X-ray investigations for 1-(2,4-dichlorophenoxy)acetyl-4-(2-fluorophenyl) thiosemicarbazide, 1-(2,4-dichlorophenoxy)acetyl-4-(4-metylothiophenyl)thiosemicarbazide and 1-(2,4-di chlorophenoxy)acetyl-4-(4-iodophenyl)thiosemicarbazide were carried out in order to confirm the synthesis pathways, identify their tautomeric forms, analyze the conformational preferences of molecules,...
View PublicationAberrantly reduced expression of miR-342-5p contributes to CCND1-associated chronic myeloid leukemia progression and imatinib resistance
-
Wu YY, Lai HF, Huang TC, et al. ,
-
Cell Death Dis ,
-
2021,
-
12(10):908
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Chronic myeloid leukemia (CML) is a myeloproliferative disorder associated with the Philadelphia chromosome, and the current standard of care is the use of tyrosine kinase inhibitors (TKI). However, some patients will not achieve a molecular response and may progress to...
View PublicationUPF1 promotes the formation of R loops to stimulate DNA double-strand break repair
-
Ngo GHP, Grimstead JW, Baird DM ,
-
Nat Commun ,
-
2021,
-
12(1):3849
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, GFP Transfection Efficiency Assay
- Research area: Cell Biology
DNA-RNA hybrid structures have been detected at the vicinity of DNA double-strand breaks (DSBs) occurring within transcriptional active regions of the genome. The induction of DNA-RNA hybrids strongly affects the repair of these DSBs, but the nature of these structures...
View PublicationCharacterization of a functional endothelial super-enhancer that regulates ADAMTS18 and angiogenesis
-
Mushimiyimana I, Niskanen H, Beter M, et al. ,
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Nucleic Acids Res ,
-
2021,
-
49(14):8078-8096
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Epithelial Cells
- Assay: Cell Cycle Assay
- Research area: Cell Biology
Super-enhancers are clusters of enhancers associated with cell lineage. They can be powerful gene-regulators and may be useful in cell-type specific viral-vector development. Here, we have screened for endothelial super-enhancers and identified an enhancer from within a cluster that conferred...
View PublicationThe role of UVA radiation in ketoprofen-mediated BRAF-mutant amelanotic melanoma cells death – A study at the cellular and molecular level
-
Banach K, Kowalska J, Rzepka Z, et al. ,
-
Toxicology in Vitro ,
-
2021,
-
72:105108
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, Cell Vitality Assay, DNA Fragmentation Assay
- Research area: Oncology
Malignant melanoma is the cause of 80% of deaths in skin cancer patients. Treatment of melanoma in the 4th stage of clinical advancement, in which inoperable metastasis occur, does not provide sufficient effects. Ketoprofen has phototoxic properties and it can...
View PublicationResponse of Human Glioblastoma Cells to Vitamin B12 Deficiency: A Study Using the Non-Toxic Cobalamin Antagonist
-
Rzepka Z, Rok J, Maszczyk M, et al. ,
-
Biology ,
-
2021,
-
10(1):69
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay
- Research area: Oncology
The most important biological function of vitamin B12 is to accomplish DNA synthesis, which is necessary for cell division. Cobalamin deficiency may be especially acute for rapidly dividing cells, such as glioblastoma cells. Therefore, cobalamin antagonists offer a medicinal potential...
View PublicationInfluence of Complexation of Thiosemicarbazone Derivatives with Cu (II) Ions on Their Antitumor Activity against Melanoma Cells
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Pitucha M, Korga-Plewko A, Czylkowska A, et al. ,
-
International Journal of Molecular Sciences ,
-
2021,
-
22(6):3104
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Oncology
A series of thiosemicarbazone derivatives was prepared and their anti-tumor activity in vitro was tested. The X-ray investigation performed for compounds T2, T3 and T5 confirmed the synthesis pathway and assumed molecular structures of analyzed thiosemicarbazones. The conformational preferences of...
View PublicationBMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia
-
Seipel K, Kopp B, Bacher U, Pabst T ,
-
Cancers ,
-
2021,
-
13(3):581
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay
- Research area: Oncology
Purpose: Prognosis for acute myeloid leukemia (AML) patients is poor, particularly in TP53 mutated AML, secondary, relapsed, and refractory AML, and in patients unfit for intensive treatment, thus highlighting an unmet need for novel therapeutic approaches. The combined use of...
View PublicationAllosteric and ATP-Competitive MEK-Inhibition in a Novel Spitzoid Melanoma Model with a RAF- and Phosphorylation-Independent Mutation
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Hegedüs L, Okumus Ö, Livingstone E, et al. ,
-
Cancers ,
-
2021,
-
13(4):829
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Spitzoid melanoma is a rare malignancy with histological characteristics similar to Spitz nevus. It has a diverse genetic background and in adults, a similarly grim clinical outcome as conventional malignant melanoma. We established a spitzoid melanoma cell line (PF130) from...
View PublicationExtended lifespan of bronchial epithelial cells maintains normal cellular phenotype and transcriptome integrity
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O'Loughlin J, Hall RJ, Bhaker S, et al. ,
-
ERJ Open Research ,
-
2021,
-
7(1):00254-2020
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay
- Research area: Gene Therapy
Genetic studies have identified several epithelial-derived genes associated with airway diseases. However, techniques used to study gene function frequently exceed the proliferative potential of primary human bronchial epithelial cells (HBECs) isolated from patients. Increased expression of the polycomb group protein...
View PublicationTGF-ß1 Induces Changes in the Energy Metabolism of White Adipose Tissue-Derived Human Adult Mesenchymal Stem/Stromal Cells In Vitro
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Olga Hahn , Lena-Christin Ingwersen , Abdelrahman Soliman , et al. ,
-
Metabolites ,
-
2020,
-
10(2):59
- Instrument: NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Cycle Assay
- Research area: Stem Cell Research
Adipose tissue plays an active role in the regulation of the body´s energy balance. Mesenchymal stem/stromal cells from adipose tissue (adMSC) are the precursor cells for repair and adipogenesis. Since the balance of the differentiation state of adipose tissue-resident cells...
View PublicationCytotoxic and proapoptotic effect of doxycycline – An in vitro study on the human skin melanoma cells.
-
Rok J, Karkoszka M, Rzepka Z, et al. ,
-
Toxicology in vitro_ an international journal published in association with BIBRA ,
-
2020,
-
65:104790
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay, Cell Vitality Assay, DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Small Molecule Drugs
Doxycycline is a semisynthetic, second generation tetracycline. Currently, it is used, among others, in the treatment of acne and skin infections. Moreover, doxycycline has many valuable nonantibiotic properties, including anti-inflammatory, immunosuppressive and anticancer effects. Recent studies showed that the drug...
View PublicationROS Overproduction Sensitises Myeloma Cells to Bortezomib-Induced Apoptosis and Alleviates Tumour Microenvironment-Mediated Cell Resistance
-
Caillot M, Zylbersztejn F, Maitre E, et al. ,
-
Cells ,
-
2020,
-
9(11):2357
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, FlexiCyte™ Assay
- Research area: Oncology
Multiple myeloma (MM) is a plasma cell neoplasm that remains incurable due to innate or acquired resistance. Although MM cells produce high intracellular levels of reactive oxygen species (ROS), we hypothesised that they could remain sensitive to ROS unbalance. We...
View PublicationHDAC Inhibition Induces PD-L1 Expression in a Novel Anaplastic Thyroid Cancer Cell Line
-
Hegedűs L, Rittler D, Garay T, et al. ,
-
Pathology & Oncology Research ,
-
2020,
-
26(4):2523-2535
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay
- Research area: Oncology
While papillary thyroid cancer (PTC) has largely favorable prognosis, anaplastic thyroid cancer (ATC) is a rare but extremely aggressive malignancy with grim clinical outcome. Even though new therapeutic options are emerging for ATC, additional preclinical models and novel combinations are...
View PublicationUVA Radiation Enhances Lomefloxacin-Mediated Cytotoxic, Growth-Inhibitory and Pro-Apoptotic Effect in Human Melanoma Cells through Excessive Reactive Oxygen Species Generation
-
Beberok A, Rzepka Z, Rok J, et al. ,
-
International Journal of Molecular Sciences ,
-
2020,
-
21(23):8937
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, Mitochondrial Potential Assay
- Research area: Oncology
Melanoma, the most dangerous type of cutaneous neoplasia, contributes to about 75% of all skin cancer-related deaths. Thus, searching for new melanoma treatment options is an important field of study. The current study was designed to assess whether the condition...
View PublicationChemical composition of Polish propolis and its antiproliferative effect in combination with Bacopa monnieri on glioblastoma cell lines
-
Moskwa J, Naliwajko SK, Markiewicz-Żukowska R, et al. ,
-
Scientific Reports ,
-
2020,
-
10(1):21127
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Oncology
Propolis and Bacopa monnieri (L.) Wettst. (Brahmi) are natural products that contain many active substances and possess anticancer properties. The aim of this study was to investigate the chemical composition of Polish propolis extract (PPE) by gas chromatography-mass spectrometry (GC-MS),...
View PublicationPotential synergistic effects of sorafenib and CP‑31398 for treating anaplastic thyroid cancer with p53 mutations
-
Wu JT, Lin CL, Huang CJ, et al. ,
-
Oncology Letters ,
-
2020,
-
19(4):3021-3026
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Thyroid cancer is the most commonly diagnosed endocrine cancer. Anaplastic thyroid cancer (ATC) is the most aggressive type of thyroid cancer and has a poor prognosis. Loss of p53 function has been reported to lead to poorly differentiated thyroid tumors;...
View PublicationMIM1 induces COLO829 melanoma cell death through mitochondrial membrane breakdown, GSH depletion, and DNA damage
-
Michalina Respondek , Artur Beberok , Zuzanna Rzepka , et al. ,
-
Fundamental and Clinical Pharmacology ,
-
2020,
-
34(1):20-31
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, Cell Vitality Assay, DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Oncology
Malignant melanoma is a high aggressive malignancy in humans and causes 60-80% of deaths from skin cancer. Defect in an intrinsic pathway of apoptosis via overexpression of Mcl-1 is responsible for malignant melanoma development and progression, and also for resistance...
View Publication2,4-Dichlorophenoxyacetic Thiosemicarbazides as a New Class of Compounds Against Stomach Cancer Potentially Intercalating With DNA
-
Monika Pitucha , Agnieszka Korga-Plewko , Pawel Kozyra , et al. ,
-
Biomolecules ,
-
2020,
-
10(2):296
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
hiosemicarbazide is a useful structural moiety that has the biological potential. Optimization of this structure can result in groundbreaking discovery of a new class of therapeutic agents. In the light of this, 1-(2,4-dichlorophenoxy)acetyl-4-(1-naphthyl)thiosemicarbazide (1) and 1,4-bis[(2,4-dichlorophenoxy)acetylthiosemicarbazide]phenyl (2) were synthesized and...
View PublicationThe Role of MITF and Mcl-1 Proteins in the Antiproliferative and Proapoptotic Effect of Ciprofloxacin in Amelanotic Melanoma Cells: In Silico and in Vitro Study
-
Artur Beberok , Jakub Rok , Zuzanna Rzepka , et al. ,
-
Toxicology in vitro ,
-
2020,
-
66:104884
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay, DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Oncology
Mcl-1 is a potent antiapoptotic protein which is amplified in many human cancer, while microphthalmia associated transcription factor (MITF) promotes cell proliferation and has pro-survival role. The study was designed to examine whether the interaction between ciprofloxacin, one of the...
View PublicationNext Generation Lipophilic Bisphosphonate Shows Antitumor Effect in Colorectal Cancer In Vitro and In Vivo
-
Marcell Baranyi , Dominika Rittler , Eszter Molnár , et al. ,
-
Pathology Oncology Research ,
-
2020,
-
26(3):1957-1969
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Bisphosphonates, despite proven antitumor effect in vitro in many tumor types, are currently used only for treatment of osteoporosis and bone metastasis. Colorectal cancer is the third most commonly diagnosed type of cancer and lacks targeted therapy for RAS or...
View PublicationCytotoxic Activity of Methanolic Fractions of Different Marrubium Spp. Against Melanoma Cells Is Independent of Antioxidant Activity and Total Phenolic Content
-
Malgorzata Kozyra , Agnieszka Korga , Marta Ostrowska , et al. ,
-
FEBS open bio ,
-
2020,
-
10(1):86-95
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
The Marrubium genus (horehound) has proved to be an abundant source of biologically active compounds, but there is little knowledge about its potential anticancer activity. Moreover, some Marrubium species have not been the subject of study in this regard. In...
View PublicationMcl-1 Inhibitor Induces Cells Death in BRAF-Mutant Amelanotic Melanoma Trough GSH Depletion, DNA Damage and Cell Cycle Changes
-
Michalina Respondek , Artur Beberok , Zuzanna Rzepka , et al. ,
-
Pathology Oncology Research ,
-
2020,
-
26(3):1465-1474
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, Cell Vitality Assay, DNA Fragmentation Assay
- Research area: Oncology
Mcl-1 is a potent antiapoptotic protein and amplifies frequently in many human cancer. Currently, it is considered that the extensively expressed of Mcl-1 protein in melanoma cells is associated with rapid tumor progression, poor prognosis and low chemosensitivity. Therefore, the...
View PublicationOvercoming Multidrug Resistance by Activating Unfolded Protein Response of the Endoplasmic Reticulum in Cisplatin-Resistant A2780/CisR Ovarian Cancer Cells
-
Euitaek Jung , Dongsoo Koh , Yoongho Lim , et al. ,
-
BMB Reports ,
-
2020,
-
53(2):88-93
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Oncology
Cisplatin is a widely used anti-cancer agent. However, the effectiveness of cisplatin has been limited by the commonly developed drug resistance. This study aimed to investigate the potential effects of endoplasmic reticulum (ER) stress to overcome drug resistance using the...
View PublicationMebendazole is unique among tubulin-active drugs in activating the MEK–ERK pathway
-
Andersson CR, Selvin T, Blom K, et al. ,
-
Scientific Reports ,
-
2020,
-
10(1):13124
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Immunology
We recently showed that the anti-helminthic compound mebendazole (MBZ) has immunomodulating activity in monocyte/macrophage models and induces ERK signalling. In the present study we investigated whether MBZ induced ERK activation is shared by other tubulin binding agents (TBAs) and if...
View PublicationStereoisomer-specific ginsenoside 20(S)-Rg3 reverses replicative senescence of human diploid fibroblasts via Akt-mTOR-Sirtuin signaling.
-
Yang KE, Jang HJ,, et al. ,
-
Journal of ginseng research ,
-
2020,
-
44(2):341-349
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Cycle Assay
- Research area: Cell Biology
Background: The replicative senescence of human dermal fibroblasts (HDFs) is accompanied by growth arrest. In our previous study, the treatment of senescent HDFs with Rg3(S) lowered the intrinsic reactive oxygen species (ROS) levels and reversed cellular senescence by inducing peroxiredoxin-3,...
View PublicationRapid Detection of Senescent Mesenchymal Stromal Cells by a Fluorescent Probe
-
Joshur Ang , Yong-An Lee , Deepak Raghothaman , et al. ,
-
Biotechnology Journal ,
-
2019,
-
14(10):e1800691
- Instrument: NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Cycle Assay
- Research area: Stem Cell Research
Despite intense interest in human mesenchymal stromal cells (MSCs), monitoring of the progressive occurrence of senescence has been hindered by the lack of efficient detection tools. Here, the discovery of a novel MSC senescence-specific fluorescent probe (CyBC9) identified by a...
View PublicationEvaluation of in Vitro Toxicity of Silica Nanoparticles (NPs) to Lung Cells: Influence of Cell Types and Pulmonary Surfactant Component DPPC
-
Jing Li , Haiyin Yang , Suinan Sha , et al. ,
-
Ecotoxicology and environmental safety ,
-
2019,
-
186:109770
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Cycle Assay
- Research area: Small Molecule Drugs
Cultured human lung epithelial cells, particularly A549 cells, are commonly used as the in vitro model to evaluate the inhalational toxicity of nanoparticles (NPs). However, A549 cells are cancer cells that might not reflect the response of normal tissues to...
View PublicationA Synthetic Chalcone Derivative, 2-hydroxy-3′,5,5′-trimethoxychalcone (DK-139), Triggers Reactive Oxygen Species-Induced Apoptosis Independently of p53 in A549 Lung Cancer Cells
-
Ha Na Gil , Euitaek Jung , Dongsoo Koh , et al. ,
-
Chemico-Biological Interactions ,
-
2019,
-
298:72-79
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Hepatocytes
- Assay: Annexin V Assay, Cell Cycle Assay, DNA Fragmentation Assay
- Research area: Oncology
2-Hydroxy-3′,5,5′-trimethoxychalcone (named DK-139) is a synthetic chalcone derivative that has anti-inflammatory, anti-tumor, and endoplasmic reticulum-mediated apoptosis activities. However, the mode of action of DK-139 on reactive oxygen species (ROS)-induced apoptosis remains unknown. In this study, we found that DK-139 activated...
View PublicationAlectinib, an Anaplastic Lymphoma Kinase Inhibitor, Abolishes ALK Activity and Growth in ALK-Positive Neuroblastoma Cells
-
Muhammad Wasi Alam , Marcus Borenäs , Dan E Lind , et al. ,
-
Frontiers in Oncology ,
-
2019,
-
0,777083333
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Neural Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Oncogenic receptor tyrosine kinases including anaplastic lymphoma kinase (ALK) are implicated in numerous solid and hematologic cancers. ALK mutations are reported in an estimated 9% of neuroblastoma and recent reports indicate that the percentage of ALK-positive cases increases in the...
View PublicationFluoxetine selectively induces p53-independent apoptosis in human colorectal cancer cells
-
Monika Marcinkute , Saeed Afshinjavid , Amos A Fatokun , Farideh A Javid ,
-
European Journal of Pharmacology ,
-
2019,
-
857:172441
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, Mitochondrial Potential Assay
- Research area: Oncology
Fluoxetine has been shown to induce anti-tumour activity. The aim of this study was to determine the effect of fluoxetine on HCT116+/+ and p53 gene-depleted HCT116-/- human colorectal cancer cells and the mechanisms, including potential p53-dependence, of its action. Fluoxetine-induced...
View PublicationMoxifloxacin as an Inducer of Apoptosis in Melanoma Cells: A Study at the Cellular and Molecular Level
-
Artur Beberok , Zuzanna Rzepka , Michalina Respondek , et al. ,
-
Toxicology in vitro ,
-
2019,
-
2019 Mar;55:75-92
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay, DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Oncology
Fluoroquinolones were shown to be cytotoxic towards various cancer cell lines, thus representing a potentially important source of new anticancer agents. The aim of the present study was to examine the effect of moxifloxacin on cell viability, redox balance and...
View PublicationExpanding the Chemical Space of Withaferin A by Incorporating Silicon To Improve Its Clinical Potential on Human Ovarian Carcinoma Cells
-
Nayra R Perestelo , Gabriel G Llanos , Carolina P Reyes , et al. ,
-
Journal of Medicinal Chemistry ,
-
2019,
-
62(9):4571-4585
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Ovarian cancer represents the seventh most commonly diagnosed cancer worldwide. Herein, we report on the development of a withaferin A (WA)-silyl ether library with 30 analogues reported for the first time. Cytotoxicity assays on human epithelial ovarian carcinoma cisplatin-sensitive and...
View PublicationCarboxylated Carbon Nanomaterials in Cell Cycle and Apoptotic Cell Death Regulation
-
Kuen-Chan Lee , Pei-Ying Lo , Guang-Yu Lee , et al. ,
-
Journal of Biotechnology ,
-
2019,
-
296:14-21
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, Cell Vitality Assay
- Research area: Oncology
Carbon nanomaterials, include carbon nanotubes and graphene nanosheets, have drawn an increasing amount of attention because of their potential applications in daily life or in providing novel therapeutic possibilities for treating diseases. However, the overall biocompatibility, the potential toxic effects...
View PublicationCobalamin Deficiency: Effect on Homeostasis of Cultured Human Astrocytes
-
Zuzanna Rzepka, Jakub Rok, Michalina Respondek, et al. ,
-
Cells ,
-
2019,
-
8(12):1505
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Cell Biology
Cobalamin deficiency is an important health problem. The major non-hematological symptoms of hypocobalaminemia are nervous system disorders, but the molecular and cellular mechanisms underlying this phenomenon have not yet been fully explained. Increasing scientific evidence is stressing the pivotal role...
View PublicationExtracellular Vesicles Induce Minimal Hepatotoxicity and Immunogenicity
-
Amer F Saleh , Elisa Lázaro-Ibáñez, Malin A-M Forsgard, et al. ,
-
Nanoscale ,
-
2019,
-
11(14):6990-7001
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Cycle Assay
- Research area: Cell Biology
Extracellular vesicles (EVs) mediate cellular communication through the transfer of active biomolecules, raising interest in using them as biological delivery vehicles for therapeutic drugs. For drug delivery applications, it is important to understand the intrinsic safety and toxicity liabilities of...
View PublicationMIM1, the Mcl-1 – specific BH3 mimetic induces apoptosis in human U87MG glioblastoma cells
-
Respondek M, Beberok A, Rok J, et al. ,
-
Toxicol In Vitro ,
-
2018,
-
53:126-135
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Small Molecule Drugs
Glioblastoma multiforme (GBM) is the most common and high aggressive malignant brain tumor. Despite evolving oncology treatment and novel chemotherapeutic agents the median survival of patients diagnosed with GBM is only 12-15 months. This grim fact highlights necessity to identify...
View PublicationCellular, transcriptomic and methylome effects of individual and combined exposure to BPA, BPF, BPS on mouse spermatocyte GC-2 cell line
-
Sidorkiewicz I, Czerniecki J, Jarząbek K, et al. ,
-
Toxicol Appl Pharmacol ,
-
2018,
-
359:1-11
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, Mitochondrial Potential Assay
- Research area: Small Molecule Drugs
Environmental factors, particularly xenoestrogens adversely affect reproductive health and their main mechanism is based on steroid-signaling pathway alterations. The presence of bisphenol A (BPA) in the environment has been confirmed and it is about to be replaced by analogues such...
View PublicationGSH depletion, mitochondrial membrane breakdown, caspase-3/7 activation and DNAfragmentation in U87MG glioblastoma cells: New insight into the mechanism of cytotoxicityinduced by fluoroquinolones
-
Beberok A, Rzepka Z, Respondek M, et al. ,
-
Eur J Pharmacol ,
-
2018,
-
835:94-107
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, Cell Vitality Assay, Mitochondrial Potential Assay
- Research area: Oncology
Fluoroquinolones are a known synthetic group of antibiotics that have been the subject of many research interests. This class of antibiotics was shown to be cytotoxic towards various cancer cell lines, thus representing a potentially important source of new anticancer...
View PublicationDesign, synthesis, biological evaluation, structure-activity relationship study, and mode of action of 2-phenol-4,6-dichlorophenyl-pyridines
-
Shrestha A, Park S, Shin S, et al. ,
-
Bioorganic Chemistry ,
-
2018,
-
79, 1-18
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Human DNA topoisomerases (Topos) are essential nuclear enzyme whose level of expression is potential indicator for prediction of responsive result of chemotherapy. Topos has become a key cellular target for most of the anticancer agents that regulates topological problems of...
View PublicationHyperactivated m-calpain affects acquisition of doxorubicin resistance in breast cancer cells
-
Jeon KH, Yu HV, Kwon Y ,
-
Biochim Biophys Acta ,
-
2018,
-
1862(5):1126-1133
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Background: Doxorubicin is commonly using chemotherapeutic agents for breast cancer. However, doxorubicin has limitations in clinical use because of dose-dependent cardiotoxicity and drug resistance. Despite of previously reported studies about mechanisms of doxorubicin resistance including overexpression of P-gp and abnormal...
View PublicationAZD8055 Exerts Antitumor Effects on Colon Cancer Cells by Inhibiting mTOR and Cell-cycleProgression
-
Chen Y, Lee CH, Tseng BY, et al. ,
-
Anticancer Res ,
-
2018,
-
38(3):1445-1454
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Background/aim: AZD8055 is an inhibitor of mammalian target of rapamycin (mTOR) that can suppress both mTOR complex 1 (mTORC1) and mTORC2. This study investigated the antitumor effects of AZD8055 on colon cancer. Materials and methods: The effects of AZD8055 on...
View PublicationGATA3 as a master regulator and therapeutic target in ovarian high-grade serous carcinoma stemcells
-
Chen HJ, Huang RL, Liew PL7, et al. ,
-
Int J Cancer ,
-
2018,
-
143(12):3106-3119
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Ovarian high-grade serous carcinoma (HGSC) is the most lethal gynecological malignancy. Prevailing evidences suggest that drug resistance and recurrence of ovarian HGSC are caused by the presence of cancer stem cells. Therefore, targeting cancer stems is appealing, however, all attempts...
View PublicationLinc00659, a long noncoding RNA, acts as novel oncogene in regulating cancer cell growth in colorectal cancer
-
Tsai KW, Lo YH, Liu H, et al. ,
-
Mol Cancer ,
-
2018,
-
17(1):72
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Oncology
Background: Colorectal cancer (CRC) is one of the most common cancers and causes of cancer-related death worldwide. In patients with CRC, metastasis is a crucial problem that leads to treatment failure and is the primary cause of the lethality of...
View PublicationPAN-cancer analysis of S-phase enriched lncRNAs identifies oncogenic drivers and biomarkers
-
Ali MM, Akhade VS, Kosalai ST, et al. ,
-
Nat Commun ,
-
2018,
-
9(1):883
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Despite improvement in our understanding of long noncoding RNAs (lncRNAs) role in cancer, efforts to find clinically relevant cancer-associated lncRNAs are still lacking. Here, using nascent RNA capture sequencing, we identify 1145 temporally expressed S-phase-enriched lncRNAs. Among these, 570 lncRNAs...
View PublicationCiprofloxacin-mediated Induction of S-phase Cell Cycle Arrest and Apoptosis in COLO829 Melanoma Cells
-
Artur Beberok , Dorota Wrześniok , Aldona Minecka , et al. ,
-
Pharmacological Reports ,
-
2018,
-
70(1):6-13
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, Mitochondrial Potential Assay
- Research area: Oncology
Background: Low effectiveness of anti-melanoma therapies makes it necessary to search for new drugs that could improve or replace the standard chemotherapy. Fluoroquinolones are a group of synthetic antibiotics, used in the treatment of wide range of bacterial infections. Moreover,...
View PublicationMDM2- and FLT3-inhibitors in the treatment of FLT3-ITD acute myeloid leukemia, specificity and efficacy of NVP-HDM201 and midostaurin
-
Seipel K, Marques MAT, Sidler C, et al. ,
-
Haematologica ,
-
2018,
-
103(11):1862-1872
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Oncology
Prognosis for FLT3-ITD positive acute myeloid leukemia with high allelic ratio (>0.5) is poor, particularly in relapse, refractory to or unfit for intensive treatment, thus highlighting an unmet need for novel therapeutic approaches. The combined use of compounds targeting both...
View PublicationGanoderma microsporum immunomodulatory protein induces apoptosis and potentiatesmitomycin C-induced apoptosis in urinary bladder urothelial carcinoma cells
-
Huang SY, Chien CC, Hseu RS, et al. ,
-
J Cell Biochem ,
-
2018,
-
119(6):4592-4606
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, Mitochondrial Potential Assay
- Research area: Oncology
Current chemotherapy and immunotherapy treatments followed by transurethral resection for urinary bladder urothelial carcinoma (UC) usually suffer from poor prognosis and high recurrence rate. Design and modification of current formulation with the novel adjuvants are needed. A recombinant protein derived...
View PublicationFluid Shear Stress Induces Cell Cycle Arrest in Human Urinary Bladder Transitional Cell Carcinoma Through Bone Morphogenetic Protein Receptor-Smad1/5 Pathway
-
Lee YH, Lai CW, Cheng YC ,
-
Cellular and Molecular Bioengineering ,
-
2018,
-
11(3):185-195
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Introduction: Mechanical force generated from the interstitial fluid flow inside and surrounding tissue has been known to play a significant role in cancer pathophysiology. In this study, we aimed to investigate the role of laminar shear stress (LSS) in modulating...
View PublicationNovel 2,3-disubstituted 1,3-thiazolidin-4-one derivatives as potential antitumor agents in renal cell adenocarcinoma
-
Gawronska-Grzywacz, M., Popiotek, et al. ,
-
Oncology Reports ,
-
2018,
-
41(1(, 693-701
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Cancer represents one of the main causes of mortality in developed countries. In particular, the overall survival of patients with renal cell carcinoma (RCC) remains poor and the available cytostatic agents are insufficient. Therefore, there is an urgent requirement to...
View PublicationPan-RAF and MEK vertical inhibition enhances therapeutic response in non-V600 BRAF mutantcells
-
Molnár E, Rittler D, Baranyi M, et al. ,
-
BMC Cancer ,
-
2018,
-
18(1):542
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Background: Currently, there are no available targeted therapy options for non-V600 BRAF mutated tumors. The aim of this study was to investigate the effects of RAF and MEK concurrent inhibition on tumor growth, migration, signaling and apoptosis induction in preclinical...
View PublicationThe Synthetic Chalcone Derivative 2-hydroxy-3′,5,5′-trimethoxychalcone Induces Unfolded Protein Response-Mediated Apoptosis in A549 Lung Cancer Cells
-
Ha-Na Gil , Dongsoo Koh , Yoongho Lim , et al. ,
-
Bioorganic and Medicinal Chemistry letters ,
-
2018,
-
28(17):2969-2975
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, DNA Fragmentation Assay
- Research area: Oncology
The synthetic chalcone derivative 2-hydroxy-3′,5,5′-trimenthoxyochalcone (named DK-139) exhibits anti-inflammatory and anti-tumor invasion properties. However, effects of DK-139 on tumor cell growth remain unknown. In the present study, we evaluated the inhibitory activity of DK-139 against human lung cancer cells. Treatment...
View PublicationThe GTPase domain of gamma-tubulin is required for normal mitochondrial function and spatial organization
-
Lindström L, Li T, Malycheva D, et al. ,
-
Communications Biology ,
-
2018,
-
01.37
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Cycle Assay
- Research area: Cell Biology
In the cell, γ-tubulin establishes a cellular network of threads named the γ-string meshwork. However, the functions of this meshwork remain to be determined. We investigated the traits of the meshwork and show that γ-strings have the ability to connect...
View PublicationIn vitro testing of estragole in HepG2 cells: Cytokinesis-block micronucleus assay and cell-cycle analysis
-
Vannini S, Pagiotti R, Acito M, et al. ,
-
Acta Alimentaria ,
-
2018,
-
47 (2)
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Hepatocytes
- Assay: Cell Cycle Assay
- Research area: Cell Biology
The alkenylbenzene estragole (systematic name, 1-allyl-4-methoxybenzene) is a natural component of essential oils from various spices and herbs, including fennel, and it is used as a food and beverage flavouring agent. Estragole has been reported to be hepatocarcinogenic at high...
View PublicationPhosphoproteome and Gene Expression Profiling of ALK Inhibition in Neuroblastoma Cell Lines Reveals Conserved Oncogenic Pathways
-
Jimmy Van den Eynden , Ganesh Umapathy , Arghavan Ashouri , et al. ,
-
Science Signaling ,
-
2018,
-
11(557):eaar5680
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Cycle Assay
- Research area: Cell Biology
Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor that is a clinical target of major interest in cancer. Mutations and rearrangements in ALK trigger the activation of the encoded receptor and its downstream signaling pathways. ALK mutations have been...
View PublicationSpecies-specific differences in peroxisome proliferation, catalase, and SOD2 upregulation as well as toxicity inhuman, mouse, and rat hepatoma cells induced by the explosive and environmental pollutant 2,4,6-trinitrotoluene
-
Naumenko EA,, Ahlemeyer B, Baumgart-Vogt E ,
-
Environ Toxicol ,
-
2017,
-
32(3):989-1006
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Hepatocytes
- Assay: Cell Cycle Assay
- Research area: Small Molecule Drugs
2,4,6-Trinitrotoluene (TNT) has been widely used as an explosive substance and its toxicity is still of interest as it persisted in polluted areas. TNT is metabolized in hepatocytes which are prone to its toxicity. Since analysis of the human liver...
View PublicationIn vitro toxicological effects of zinc containing nanoparticles with different physico-chemicalproperties
-
Uski O, Torvela T, Sippula O, et al. ,
-
Toxicol In Vitro ,
-
2017,
-
42:105-113
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Eukaryotic Cells
- Assay: Cell Cycle Assay
- Research area: Small Molecule Drugs
Nanomaterials (NM) exhibit novel physicochemical properties that determine their interaction with biological substrates and processes. Recent nano-technological advances are leading to wide usage of metallic nanoparticles (NPs) in various fields. However, the increasing use of NPs has led to their...
View PublicationTargeting TPX2 Suppresses the Tumorigenesis of Hepatocellular Carcinoma Cells Resulting in Arrested Mitotic Phase Progression and Increased Genomic Instability
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Hsu CW, Yu-Chia Chen, Hsing-Hao Su, et al. ,
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J of Cancer ,
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2017,
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8(8): 1378-1394
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Hepatocytes
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Oncology
Hepatocellular carcinoma (HCC) remains one of the most difficult cancers to treat, with chemotherapies being relatively ineffective. Therefore, a better knowledge of molecular hepatocarcinogenesis will provide opportunities for designing targeted therapies. TPX2 (targeting protein for Xklp2) is overexpressed as a...
View PublicationTRAIL-functionalized gold nanoparticles selectively trigger apoptosis in polarized macrophag
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Huang YJ, Shan-hui Hsu ,
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Nanotheranostics ,
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2017,
-
1(3):326-337
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Leukocytes
- Assay: Cell Cycle Assay, Cell Vitality Assay
- Research area: Oncology
Tumor-associated macrophages (TAMs) have the same immunosuppressive effects as M2 macrophages in tumor progression and are correlated with poor-patient prognosis and survival in non-small cell lung cancer (NSCLC). Therefore, TAMs are the potential targets for cancer therapy. Tumor necrosis factor-related...
View PublicationTargeting the PI3K/AKT/mTOR signaling pathway as an effectively radiosensitizing strategy for treating human oral squamous cell carcinoma in vitro and in vivo
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Yu CC, Hung SK, Lin HY, et al. ,
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Oncotarget ,
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2017,
-
8(40):68641-68653
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Radiation therapy (RT) is the current standard adjuvant approach for oral squamous cell carcinoma (OSCC) patients. Radioresistance is a major contributor to radiotherapy failure. In this study, we used patient-derived cells and a radiation-resistant cell line in vitro and in...
View PublicationTargeted TPX2 increases chromosome missegregation and suppresses tumor cell growth in human prostate cancer
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Pan HW,, Su HH, et al. ,
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Onco Targets Ther ,
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2017,
-
10:3531-3543
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Oncology
Prostate cancer is a complex disease that can be relatively harmless or extremely aggressive. Although androgen-deprivation therapy is a commonly used treatment for men with prostate cancer, the adverse effects can be detrimental to patient health and quality of life....
View PublicationThe Functional Roles of the MDM2 Splice Variants P2-MDM2-10 and MDM2-∆5 in Breast CancerCells
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Huun J, Gansmo LB, Mannsåker B, et al. ,
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Transl Oncol ,
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2017,
-
10(5):806-817
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay, GFP Transfection Efficiency Assay
- Research area: Oncology
Background: MDM2 is a negative regulator of p53 and is upregulated in numerous human cancers. While different MDM2 splice variants have been observed in both normal tissues and malignant cells, their functions are poorly understood. Methods: We evaluated the effect...
View PublicationImpact of the MDM2 splice-variants MDM2-A, MDM2-B and MDM2-C on cytotoxic stress response in breast cancer cells
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Huun J, Gansmo LB, Mannsåker B, et al. ,
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BMC Cell Biol ,
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2017,
-
18(1):17
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Oncology
Background: The murine double minute 2 (MDM2) is an oncogene and a negative regulator of the tumor suppressor protein p53. MDM2 is known to be amplified in numerous human cancers, and upregulation of MDM2 is considered to be an alternative...
View PublicationStructural optimization and evaluation of novel 2-pyrrolidone-fused (2-oxoindolin-3-ylidene)methylpyrrole derivatives as potential VEGFR-2/PDGFRβ inhibitors
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Yang TH, Chun-I Lee, Wen-Hsin Huang and An-Rong Lee ,
-
Chemistry Central Journal ,
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2017,
-
0,508333333
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Background: Tumor angiogenesis, essential for tumor growth and metastasis, is tightly regulated by VEGF/VEGFR and PDGF/PDGFR pathways, and therefore blocking those pathways is a promising therapeutic target. Compared to sunitinib, the C(5)-Br derivative of 2-pyrrolidone-fused (2-oxoindolin-3-ylidene)methylpyrrole has significantly greater in...
View PublicationAurora kinase A inhibitor TCS7010 demonstrates pro‑apoptotic effect through the unfolded protein response pathway in HCT116 colon cancer cells
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Lee DH, Chang Gun Kim, Yoongho Lim, Soon Young Shin ,
-
Oncology Letters ,
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2017,
-
14(6):6571-6577
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Oncology
Aurora kinase A (AURKA) is essential for regulating mitosis and is frequently amplified in various cancer cell types. However, the effect of AURKA inhibition on the induction of apoptosis remains unclear. In the present study, it was reported that treatment...
View PublicationApplication of NucleoCounter for the Comprehensive Assessment of Murine Cultured Neurons During Infection With Equine Herpesvirus Type 1 (EHV-1)
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M Chodkowski , I Serafińska , J Brzezicka , et al. ,
-
Polish Journal of Veterinary Sciences ,
-
2017,
-
20(4):831-834
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Neural Cells
- Assay: Cell Cycle Assay, Cell Vitality Assay
- Research area: Neurobiology
The NucleoCounter NC-3000, a portable high-speed cell counting device based on the principle of fluorescence microscopy, provides the alternative method for standard flow cytometry. The main objective of the study was to apply an efficient technique for the assessment of...
View PublicationSynthesis and Evaluation of Novel 2-Pyrrolidone-Fused (2-Oxoindolin-3-ylidene)methylpyrroleDerivatives as Potential Multi-Target Tyrosine Kinase Receptor Inhibitors
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Yang TH, Lee CI, Huang WH, Lee AR ,
-
Molecules ,
-
2017,
-
22(6). pii: E913
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Signaling pathways of VEGFs and PDGFs are crucial in tumor angiogenesis, which is essential in solid tumor progression and metastasis. This study reports our strategy for designing and synthesizing a series of novel 2-pyrrolidone-fused (2-oxoindolin-3-ylidene)methylpyrrole derivatives as potential multi-target tyrosine...
View PublicationReduced cell size, chromosomal aberration and altered proliferation rates are characteristics and confounding factors in the STHdh cell model of Huntington disease
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Singer E, Walter C, Weber JJ, et al. ,
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Sci Rep ,
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2017,
-
7(1):16880
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Eukaryotic Cells
- Assay: Cell Cycle Assay
- Research area: Cell Biology
Huntington disease is a fatal neurodegenerative disorder caused by a CAG repeat expansion in the gene encoding the huntingtin protein. Expression of the mutant protein disrupts various intracellular pathways and impairs overall cell function. In particular striatal neurons seem to...
View PublicationThe LXCXE Retinoblastoma Protein-Binding Motif of FOG-2 Regulates Adipogenesis
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Goupille O, Penglong T, Kadri Z, et al. ,
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Cell Rep ,
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2017,
-
21(12):3524-3535
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Cycle Assay
- Research area: Cell Biology
GATA transcription factors and their FOG cofactors play a key role in tissue-specific development and differentiation, from worms to humans. Mammals have six GATA and two FOG factors. We recently demonstrated that interactions between retinoblastoma protein (pRb) and GATA-1 are...
View PublicationAxl-EGFR receptor tyrosine kinase hetero-interaction provides EGFR with access to pro-invasive signalling in cancer cells.
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Vouri M, Croucher DR,, et al. ,
-
Oncogenesis ,
-
2016,
-
5(10):e266
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
AZD2014 Radiosensitizes Oral Squamous Cell Carcinoma by Inhibiting AKT/mTOR Axis and Inducing G1/G2/MCell Cycle Arrest
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Yu CC, Huang HB, Hung SK, et al. ,
-
PLoS One ,
-
2016,
-
11(3):e0151942
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
A synthetic chalcone, 2′-hydroxy-2,3,5′-trimethoxychalcone triggers unfolded protein response-mediated apoptosis in breast cancer cells.
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Lee DH, Jung Jung Y, Koh D, et al. ,
-
Cancer Lett. ,
-
2016,
-
372(1):1-9.
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology