Cell Cycle Assay

Name: ChemoMetec
Price range: $

Analyze Fixed or Live Cells in Two Simple Steps Using the Cell Cycle Assay

Fast and easy measurement of cell cycle phases
Single-step acquisition, analysis, and data presentation
Standardized results – even between different users
No RNase treatment required
No calibration required
Clear data presentation and management
Data export in FCS/ACS formats

Overview of the Cell Cycle Assay

Cell cycle and cell division represent the most fundamental and important processes in eukaryotic cells. With the Cell Cycle Assay, DAPI fluorescence intensities, representative of individual cell cycle phases, are quantified and displayed in a user-friendly interface.

The 2-Step Cell Cycle Assay (upper flow) facilitates detaching, permeabilization, de-clumping and homogenous staining of the cell population in two simple steps without trypsinization, washing or centrifugation. The Cell Cycle of Fixed Cells Assay (lower flow) enables cell storage for several weeks. Thus, after ethanol fixation, the cells can be retained in cold storage for a much longer period before sample analysis.

With the NucleoCounter^® NC-3000™ cell cycle analysis, simply prepare your sample by lysis or fixation, then add staining buffers, load the sample, and press run. After the one-minute data acquisition and analysis is complete, the cell cycle profile data and percentage or number of events in G0/G1, S, G2 or sub-G1 phases displays in NucleoView™ software’s Plot Manager feature.

With the FlexiCyte™ software package, the NC-3000™ can even be used for studying cell proliferation using BrdU- or EdU-incorporation. When detected by fluorescently labeled antibodies or Click Chemistry, these allow for advanced studies of cell proliferation.

Assay Principle

The Cell Cycle Assay uses the nuclear stain DAPI to measure DNA content. DAPI binds specifically to double-stranded DNA. Consequently, there is no requirement to remove RNA prior to DNA content measurements. This is a prerequisite for other dyes commonly used for measurement of cell cycle phases, such as propidium iodide (PI).

Using fluorescence microscopy and image analysis, DNA content quantification and measurements of cell cycle phases are automated.

Sample preparations can be loaded into either of two slide types:

Samples are analyzed using the NucleoCounter^® NC-3000™ advanced image cytometer where cellular fluorescence is quantified into histograms displaying the DNA content quantification. Markers in the displayed histograms, M1 – M4, can be used to identify cells in different cell cycle stages: sub-G1-phase, G0/G1-phase, S-phase and G2/M-phase, respectively.

Results Presented in Plot Manager

The histogram plots depict U2OS cells grown in absence (upper row) or presence (lower row) of etoposide. DNA content was measured using the 2-Step Cell Cycle Assay in the NC-3000™. Scatter plots, histograms and tables were obtained using NucleoView™ NC-3000™ software.

Markers in the histograms were used to demarcate cells in the different cell cycle phases. The colored histogram is a merge of untreated (blue line) and etoposide treated (red line) samples. G0/G1 indicated by the M1 marker, S phase indicated by M2, and the G2/M phase cells indicated by M3.

Cell Cycle Assay

Analyze Fixed or Live Cells in Two Simple Steps Using the Cell Cycle Assay

Overview of the Cell Cycle Assay

Assay Principle

Results Presented in Plot Manager

Oncology

Cell Biology

Apoptosis in Colorectal & Ovarian Cancer

Targeted Cancer Diagnoses & Therapies

Image vs. flow: a cell cycle comparison

Cell Counting in GMP Environments

Dead or Alive? Is Viability Alone Enough?

Science Talk: Fast-Tracking a COVID-19 Vaccine

NC-Slide A2™

NC-Slide A8™

Instrument Solutions

Tech notes

Tech Note: Variations and Statistics for NucleoCounter® NC-3000™

White Paper: NucleoView™ NC-3000™ Software and CFR 21 Part 11

Tech Note: LED Life Time Consideration for NucleoCounter® NC-3000™

Publications

Allosteric and ATP-Competitive MEK-Inhibition in a Novel Spitzoid Melanoma Model with a RAF- and Phosphorylation-Independent Mutation

The role of UVA radiation in ketoprofen-mediated BRAF-mutant amelanotic melanoma cells death – A study at the cellular and molecular level

Response of Human Glioblastoma Cells to Vitamin B12 Deficiency: A Study Using the Non-Toxic Cobalamin Antagonist

Influence of Complexation of Thiosemicarbazone Derivatives with Cu (II) Ions on Their Antitumor Activity against Melanoma Cells

HDAC Inhibition Induces PD-L1 Expression in a Novel Anaplastic Thyroid Cancer Cell Line

UVA Radiation Enhances Lomefloxacin-Mediated Cytotoxic, Growth-Inhibitory and Pro-Apoptotic Effect in Human Melanoma Cells through Excessive Reactive Oxygen Species Generation

ROS Overproduction Sensitises Myeloma Cells to Bortezomib-Induced Apoptosis and Alleviates Tumour Microenvironment-Mediated Cell Resistance

BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia

2,4-Dichlorophenoxyacetic Thiosemicarbazides as a New Class of Compounds Against Stomach Cancer Potentially Intercalating With DNA

Potential synergistic effects of sorafenib and CP‑31398 for treating anaplastic thyroid cancer with p53 mutations

Chemical composition of Polish propolis and its antiproliferative effect in combination with Bacopa monnieri on glioblastoma cell lines

MIM1 induces COLO829 melanoma cell death through mitochondrial membrane breakdown, GSH depletion, and DNA damage

Mcl-1 Inhibitor Induces Cells Death in BRAF-Mutant Amelanotic Melanoma Trough GSH Depletion, DNA Damage and Cell Cycle Assay Changes

The Role of MITF and Mcl-1 Proteins in the Antiproliferative and Proapoptotic Effect of Ciprofloxacin in Amelanotic Melanoma Cells: In Silico and in Vitro Study

Next Generation Lipophilic Bisphosphonate Shows Antitumor Effect in Colorectal Cancer In Vitro and In Vivo

Overcoming Multidrug Resistance by Activating Unfolded Protein Response of the Endoplasmic Reticulum in Cisplatin-Resistant A2780/CisR Ovarian Cancer Cells

Cytotoxic Activity of Methanolic Fractions of Different Marrubium Spp. Against Melanoma Cells Is Independent of Antioxidant Activity and Total Phenolic Content

Alectinib, an Anaplastic Lymphoma Kinase Inhibitor, Abolishes ALK Activity and Growth in ALK-Positive Neuroblastoma Cells

A Synthetic Chalcone Derivative, 2-hydroxy-3′,5,5′-trimethoxychalcone (DK-139), Triggers Reactive Oxygen Species-Induced Apoptosis Independently of p53 in A549 Lung Cancer Cells

Moxifloxacin as an Inducer of Apoptosis in Melanoma Cells: A Study at the Cellular and Molecular Level

Fluoxetine selectively induces p53-independent apoptosis in human colorectal cancer cells

Carboxylated Carbon Nanomaterials in Cell Cycle Assay and Apoptotic Cell Death Regulation

Expanding the Chemical Space of Withaferin A by Incorporating Silicon To Improve Its Clinical Potential on Human Ovarian Carcinoma Cells

Hyperactivated m-calpain affects acquisition of doxorubicin resistance in breast cancer cells

Design, synthesis, biological evaluation, structure-activity relationship study, and mode of action of 2-phenol-4,6-dichlorophenyl-pyridines

GSH depletion, mitochondrial membrane breakdown, caspase-3/7 activation and DNAfragmentation in U87MG glioblastoma cells: New insight into the mechanism of cytotoxicityinduced by fluoroquinolones

AZD8055 Exerts Antitumor Effects on Colon Cancer Cells by Inhibiting mTOR and Cell-cycleProgression

PAN-cancer analysis of S-phase enriched lncRNAs identifies oncogenic drivers and biomarkers

Ciprofloxacin-mediated Induction of S-phase Cell Cycle Assay Arrest and Apoptosis in COLO829 Melanoma Cells

GATA3 as a master regulator and therapeutic target in ovarian high-grade serous carcinoma stemcells

Linc00659, a long noncoding RNA, acts as novel oncogene in regulating cancer cell growth in colorectal cancer

Pan-RAF and MEK vertical inhibition enhances therapeutic response in non-V600 BRAF mutantcells

The Synthetic Chalcone Derivative 2-hydroxy-3′,5,5′-trimethoxychalcone Induces Unfolded Protein Response-Mediated Apoptosis in A549 Lung Cancer Cells

MDM2- and FLT3-inhibitors in the treatment of FLT3-ITD acute myeloid leukemia, specificity and efficacy of NVP-HDM201 and midostaurin

Ganoderma microsporum immunomodulatory protein induces apoptosis and potentiatesmitomycin C-induced apoptosis in urinary bladder urothelial carcinoma cells

Fluid Shear Stress Induces Cell Cycle Assay Arrest in Human Urinary Bladder Transitional Cell Carcinoma Through Bone Morphogenetic Protein Receptor-Smad1/5 Pathway

TRAIL-functionalized gold nanoparticles selectively trigger apoptosis in polarized macrophag

Novel 2,3-disubstituted 1,3-thiazolidin-4-one derivatives as potential antitumor agents in renal cell adenocarcinoma

Targeting the PI3K/AKT/mTOR signaling pathway as an effectively radiosensitizing strategy for treating human oral squamous cell carcinoma in vitro and in vivo

Targeted TPX2 increases chromosome missegregation and suppresses tumor cell growth in human prostate cancer

Targeting TPX2 Suppresses the Tumorigenesis of Hepatocellular Carcinoma Cells Resulting in Arrested Mitotic Phase Progression and Increased Genomic Instability

The Functional Roles of the MDM2 Splice Variants P2-MDM2-10 and MDM2-∆5 in Breast CancerCells

Impact of the MDM2 splice-variants MDM2-A, MDM2-B and MDM2-C on cytotoxic stress response in breast cancer cells

Synthesis and Evaluation of Novel 2-Pyrrolidone-Fused (2-Oxoindolin-3-ylidene)methylpyrroleDerivatives as Potential Multi-Target Tyrosine Kinase Receptor Inhibitors

Structural optimization and evaluation of novel 2-pyrrolidone-fused (2-oxoindolin-3-ylidene)methylpyrrole derivatives as potential VEGFR-2/PDGFRβ inhibitors

Aurora kinase A inhibitor TCS7010 demonstrates pro‑apoptotic effect through the unfolded protein response pathway in HCT116 colon cancer cells

Axl-EGFR receptor tyrosine kinase hetero-interaction provides EGFR with access to pro-invasive signalling in cancer cells.

AZD2014 Radiosensitizes Oral Squamous Cell Carcinoma by Inhibiting AKT/mTOR Axis and Inducing G1/G2/MCell Cycle Arrest

A synthetic chalcone, 2′-hydroxy-2,3,5′-trimethoxychalcone triggers unfolded protein response-mediated apoptosis in breast cancer cells.

Gene expression profiling combined with functional analysis identify integrin beta1 (ITGB1) as a potentialprognosis biomarker in triple negative breast cancer.

Characterization of preneoplastic and neoplastic rat mesothelial cell lines: the involvement of TETs, DNMTs, and 5-hydroxymethylcytosine

The natural flavone eupatorin induces Cell Cycle Assay arrest at the G2/M phase and apoptosis in HeLa cells

Anticancer and structure-activity relationship evaluation of 3-(naphthalen-2-yl)-N,5-diphenyl-pyrazoline-1-carbothioamide analogs of chalcone

Colorectal anticancer activities of polymethoxylated 3-naphthyl-5-phenylpyrazoline-carbothioamides

p53-dependent and -independent mechanisms are involved in (E)-1-(2-hydroxyphenyl)-3-(2-methoxynaphthalen-1-yl)prop-2-en-1-one (HMP)-induced apoptosis in HCT116 colon cancer cells.

A methoxyflavanone derivative, 2′,3′,4′-trimethoxy-5,6-naphthoflavanone, inhibits proliferation of HCT116 human colon cancer cells by inducing G2/M Cell Cycle Assay arrest and apoptosis

Cdc6 cooperates with c-Myc to promote genome instability and epithelial to mesenchymal transition EMT inzebrafish

RAD001 enhances the radiosensitivity of SCC4 oral cancer cells by inducing cell cycle arrest at the G2/Mcheckpoint.

A new synthetic 2′-hydroxy-2,4,6-trimethoxy-5′,6′-naphthochalcone induces G2/M cell cycle arrest and apoptosisby disrupting the microtubular network of human colon cancer cells.

Apoptosis in
Colorectal &
Ovarian Cancer

Targeted Cancer Diagnoses
& Therapies

Image vs. flow:
a cell cycle
comparison

Cell Counting in
GMP Environments