Mitochondrial Potential Assay
Assessing Apoptosis Using the Lipophilic Dye JC-1
- Easily distinguish polarized (healthy) cells, depolarized (apoptotic) cells and necrotic/late-stage apoptotic cells
- Fast, automated single cell analysis
- Acquisition and analysis in one simple step
- Standardized results – even with different users
- No calibration required
- Clear data presentation with Plot Manager feature
- Automated PDF reports
- Export data in FCS/ACS formats
Overview of the Mitochondrial Potential Assay
The lipophilic cationic dye JC-1 can be used to evaluate the mitochondrial potential. In healthy cells, the negative charge established by the intact mitochondrial membrane potential facilitates the accumulation of JC-1 in the mitochondrial matrix.
Loss of the mitochondrial membrane potential is known to precede apoptosis and chemical hypoxia-induced necrosis. JC-1 displays potential-dependent accumulation in the mitochondria and has dual-fluorescent properties: Red fluorescence indicates the dye has formed aggregates, while green fluorescence indicates the dye is monomeric. This is a simple, fluorescent-based method for distinguishing healthy cells (staining red) from apoptotic cells (staining green) in a sample.
Assay Principle
Using fluorescence microscopy and image analysis, the NucleoCounter® NC-3000™ advanced image cytometer automatically detects cells with collapsed mitochondrial membrane potential. Cells are stained with JC-1 and DAPI (Solution 7 and Solution 8, respectively).
Cellular JC-1 monomers and aggregates are detected as green and red fluorescence intensity, respectively. Mitochondrial depolarization is revealed as a decrease in the red/green fluorescence ratio. Necrotic and late apoptotic cells are detected as blue fluorescent (i.e. DAPI positive) cells.
Results Presented in Plot Manager
The diagram demonstrates the effect of adding camptothecin (CPT) on Jurkat cells. CPT is a commonly used topoisomerase poison, which induces apoptosis, thus disrupting the mitochondrial potential of cells. Jurkat cells are an immortalized line of human T lymphocyte cells, commonly used for studying the effectiveness of potential anti-cancer compounds.
Jurkat cells were grown in the absence (upper row) or in the presence (lower row) of camptothecin (CPT). Cells were stained with JC-1 and DAPI and analyzed using the Mitochondrial Potential Assay. Scatter plots and histograms were obtained from the NucleoView™ NC-3000™ software.
Polygons and markers in the displayed plots are used to demarcate the various cell populations. In this example, 9% of untreated cells are depolarized/apoptotic, whereas 61% of CPT-treated cells are depolarized/apoptotic. These findings suggest that CPT has a strong effect on mitochondrial potential.
Tech notes
Publications
Equid Alphaherpesvirus 1 (EHV-1) Influences Morphology and Function of Neuronal Mitochondria In Vitro
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Chodkowski M, Słońska A, Gregorczyk-Zboroch K, et al. ,
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Pathogens ,
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2022,
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11(8):876
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Neural Cells
- Assay: Mitochondrial Potential Assay
- Research area: Neurobiology
Mitochondria are key cellular organelles responsible for many essential functions, including ATP production, ion homeostasis and apoptosis induction. Recent studies indicate their significant role during viral infection. In the present study, we examined the effects of equine herpesvirus type 1...
View PublicationChemosensitization of U-87 MG Glioblastoma Cells by Neobavaisoflavone towards Doxorubicin and Etoposide
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Maszczyk M, Banach K, Karkoszka M, et al. ,
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Int J Mol Sci ,
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2022,
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23(10):5621
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, Mitochondrial Potential Assay
- Research area: Oncology
Glioblastoma (GB) is the most common type of glioma, which is distinguished by high mortality. Due to the rapid progression of the tumor and drug resistance, the treatment is often ineffective. The development of novel therapies in a big part...
View PublicationInvestigation of the cytotoxicity induced by didocosahexaenoin, an omega 3 derivative, in human prostate carcinoma cell lines
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Robinson GF, Sooda KKY, Phillips RM, et al. ,
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Current Research in Pharmacology and Drug Discovery ,
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2022,
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3(100085)
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay, Mitochondrial Potential Assay
- Research area: Oncology
The aim of the present study was to investigate the cytotoxicity induced by an omega-3 derivative, didocosahexaenoin (Dido) on human prostate carcinoma cells and to compare the cytotoxicity to that of docosahexaenoic acid (DHA). Different carcinoma- and non-carcinoma cells were...
View PublicationThe Anticancer Potential of Doxycycline and Minocycline-A Comparative Study on Amelanotic Melanoma Cell Lines
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Rok J, Rzepka Z, Kowalska J, et al. ,
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Int J Mol Sci ,
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2022,
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23(2):831
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, Cell Vitality Assay, Mitochondrial Potential Assay
- Research area: Oncology
Malignant melanoma is still a serious medical problem. Relatively high mortality, a still-growing number of newly diagnosed cases, and insufficiently effective methods of therapy necessitate melanoma research. Tetracyclines are compounds with pleiotropic pharmacological properties. Previously published studies on melanotic melanoma...
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Negraes PD, Trujillo CA, Yu NK, et al. ,
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Mol Psychiatry ,
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2021,
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2021 Apr 22
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Neural Cells
- Assay: DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Neurobiology
Early-onset epileptic encephalopathies are severe disorders often associated with specific genetic mutations. In this context, the CDKL5 deficiency disorder (CDD) is a neurodevelopmental condition characterized by early-onset seizures, intellectual delay, and motor dysfunction. Although crucial for proper brain development, the...
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Park MW, Cha HW, Kim J, et al. ,
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Redox Biology ,
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2021,
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41:101947
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Mitochondrial Potential Assay
- Research area: Cell Biology
Oxidative stress has been implicated in the pathogenesis of Alzheimer’s disease (AD). Mitochondrial dysfunction is linked to oxidative stress and reactive oxygen species (ROS) in neurotoxicity during AD. Impaired mitochondrial metabolism has been associated with mitochondrial dysfunction in brain damage...
View PublicationCytotoxic and proapoptotic effect of doxycycline – An in vitro study on the human skin melanoma cells.
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Rok J, Karkoszka M, Rzepka Z, et al. ,
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Toxicology in vitro_ an international journal published in association with BIBRA ,
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2020,
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65:104790
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay, Cell Vitality Assay, DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Small Molecule Drugs
Doxycycline is a semisynthetic, second generation tetracycline. Currently, it is used, among others, in the treatment of acne and skin infections. Moreover, doxycycline has many valuable nonantibiotic properties, including anti-inflammatory, immunosuppressive and anticancer effects. Recent studies showed that the drug...
View PublicationUVA Radiation Enhances Lomefloxacin-Mediated Cytotoxic, Growth-Inhibitory and Pro-Apoptotic Effect in Human Melanoma Cells through Excessive Reactive Oxygen Species Generation
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Beberok A, Rzepka Z, Rok J, et al. ,
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International Journal of Molecular Sciences ,
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2020,
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21(23):8937
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, Mitochondrial Potential Assay
- Research area: Oncology
Melanoma, the most dangerous type of cutaneous neoplasia, contributes to about 75% of all skin cancer-related deaths. Thus, searching for new melanoma treatment options is an important field of study. The current study was designed to assess whether the condition...
View PublicationMIM1 induces COLO829 melanoma cell death through mitochondrial membrane breakdown, GSH depletion, and DNA damage
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Michalina Respondek , Artur Beberok , Zuzanna Rzepka , et al. ,
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Fundamental and Clinical Pharmacology ,
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2020,
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34(1):20-31
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, Cell Vitality Assay, DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Oncology
Malignant melanoma is a high aggressive malignancy in humans and causes 60-80% of deaths from skin cancer. Defect in an intrinsic pathway of apoptosis via overexpression of Mcl-1 is responsible for malignant melanoma development and progression, and also for resistance...
View PublicationHeterobasidion annosum Induces Apoptosis in DLD-1 Cells and Decreases Colon Cancer Growth in In Vivo Model
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Anna Sadowska , Ewa Zapora , Diana Sawicka , et al. ,
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International Journal of Molecular Sciences ,
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2020,
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21(10):E3447
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Vitality Assay, Mitochondrial Potential Assay
- Research area: Oncology
Application of substances from medicinal mushrooms is one of the interesting approaches to improve cancer therapy. In this study, we commenced a new attempt in the field of Heterobasidion annosum (Fr.) Bref. sensu lato to further extend our knowledge on...
View PublicationThe Role of MITF and Mcl-1 Proteins in the Antiproliferative and Proapoptotic Effect of Ciprofloxacin in Amelanotic Melanoma Cells: In Silico and in Vitro Study
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Artur Beberok , Jakub Rok , Zuzanna Rzepka , et al. ,
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Toxicology in vitro ,
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2020,
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66:104884
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay, DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Oncology
Mcl-1 is a potent antiapoptotic protein which is amplified in many human cancer, while microphthalmia associated transcription factor (MITF) promotes cell proliferation and has pro-survival role. The study was designed to examine whether the interaction between ciprofloxacin, one of the...
View PublicationIncreased antioxidant response in medium-chain acyl-CoA dehydrogenase deficiency: does lipoic acid have a protective role?
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Nochi Z, Birkler RID, Fernandez-Guerra P, et al. ,
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Pediatric Research ,
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2020,
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88(4):556-564
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Mitochondrial Potential Assay
- Research area: Cell Biology
Background: Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency (MCADD) is the most frequent fatty acid oxidation (FAO) defect in humans. MCAD-deficient fibroblasts are more resistant to oxidative stress-induced cell death than other FAO defects and healthy controls. Methods: Herein we investigate the...
View PublicationFluoxetine selectively induces p53-independent apoptosis in human colorectal cancer cells
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Monika Marcinkute , Saeed Afshinjavid , Amos A Fatokun , Farideh A Javid ,
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European Journal of Pharmacology ,
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2019,
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857:172441
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, Mitochondrial Potential Assay
- Research area: Oncology
Fluoxetine has been shown to induce anti-tumour activity. The aim of this study was to determine the effect of fluoxetine on HCT116+/+ and p53 gene-depleted HCT116-/- human colorectal cancer cells and the mechanisms, including potential p53-dependence, of its action. Fluoxetine-induced...
View PublicationMoxifloxacin as an Inducer of Apoptosis in Melanoma Cells: A Study at the Cellular and Molecular Level
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Artur Beberok , Zuzanna Rzepka , Michalina Respondek , et al. ,
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Toxicology in vitro ,
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2019,
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2019 Mar;55:75-92
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay, DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Oncology
Fluoroquinolones were shown to be cytotoxic towards various cancer cell lines, thus representing a potentially important source of new anticancer agents. The aim of the present study was to examine the effect of moxifloxacin on cell viability, redox balance and...
View PublicationDisturbances of Mitochondrial Dynamics in Cultured Neurons Infected With Human Herpesvirus Type 1 and Type 2
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Joanna Cymerys , Marcin Chodkowski , Anna Słońska , et al. ,
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Journal of Neurovirology ,
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2019,
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25(6):765-782
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Neural Cells
- Assay: Cell Vitality Assay, Mitochondrial Potential Assay
- Research area: Neurobiology
Human herpesvirus types 1 and 2 (HHV-1 and HHV-2) are neurotropic viruses which remain latent for life and reactivate to cause recurrent infections. HHV-1 has been found to be involved in accumulation of β-amyloid, hyperphosphorylation of tau proteins, and inflammation...
View PublicationCobalamin Deficiency: Effect on Homeostasis of Cultured Human Astrocytes
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Zuzanna Rzepka, Jakub Rok, Michalina Respondek, et al. ,
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Cells ,
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2019,
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8(12):1505
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Cell Biology
Cobalamin deficiency is an important health problem. The major non-hematological symptoms of hypocobalaminemia are nervous system disorders, but the molecular and cellular mechanisms underlying this phenomenon have not yet been fully explained. Increasing scientific evidence is stressing the pivotal role...
View PublicationMIM1, the Mcl-1 – specific BH3 mimetic induces apoptosis in human U87MG glioblastoma cells
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Respondek M, Beberok A, Rok J, et al. ,
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Toxicol In Vitro ,
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2018,
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53:126-135
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Small Molecule Drugs
Glioblastoma multiforme (GBM) is the most common and high aggressive malignant brain tumor. Despite evolving oncology treatment and novel chemotherapeutic agents the median survival of patients diagnosed with GBM is only 12-15 months. This grim fact highlights necessity to identify...
View PublicationCellular, transcriptomic and methylome effects of individual and combined exposure to BPA, BPF, BPS on mouse spermatocyte GC-2 cell line
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Sidorkiewicz I, Czerniecki J, Jarząbek K, et al. ,
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Toxicol Appl Pharmacol ,
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2018,
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359:1-11
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, Mitochondrial Potential Assay
- Research area: Small Molecule Drugs
Environmental factors, particularly xenoestrogens adversely affect reproductive health and their main mechanism is based on steroid-signaling pathway alterations. The presence of bisphenol A (BPA) in the environment has been confirmed and it is about to be replaced by analogues such...
View PublicationGSH depletion, mitochondrial membrane breakdown, caspase-3/7 activation and DNAfragmentation in U87MG glioblastoma cells: New insight into the mechanism of cytotoxicityinduced by fluoroquinolones
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Beberok A, Rzepka Z, Respondek M, et al. ,
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Eur J Pharmacol ,
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2018,
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835:94-107
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, Cell Vitality Assay, Mitochondrial Potential Assay
- Research area: Oncology
Fluoroquinolones are a known synthetic group of antibiotics that have been the subject of many research interests. This class of antibiotics was shown to be cytotoxic towards various cancer cell lines, thus representing a potentially important source of new anticancer...
View PublicationThe Proto-oncogene c-Kit Inhibits Tumor Growth by Behaving as a Dependence Receptor
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Wang H, Boussouar A, Mazelin L, et al. ,
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Mol Cell ,
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2018,
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72(3):413-425.e5
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Immune Cells
- Assay: Mitochondrial Potential Assay
- Research area: Oncology
c-Kit is a classic proto-oncogene either mutated or upregulated in cancer cells, and this leads to its constitutive kinase activation and, thus, to uncontrolled proliferation. Although the pro-oncogenic role of c-Kit is of no doubt, some observations do not fit...
View PublicationHuman herpesvirus type 1 and type 2 disrupt mitochondrial dynamics in human keratinocytes
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Chodkowski M, Serafińska I, Brzezicka J, et al. ,
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Arch Virol ,
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2018,
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163(10):2663-2673
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Vitality Assay, Mitochondrial Potential Assay
- Research area: Oncology
Mitochondrial movement and distribution throughout the cytoplasm is crucial for maintaining cell homeostasis. Mitochondria are dynamic organelles but can be functionally disrupted during infection. Here, we show that the ubiquitous human pathogens HHV-1 and HHV-2 induce changes in the mitochondrial...
View PublicationCiprofloxacin-mediated Induction of S-phase Cell Cycle Arrest and Apoptosis in COLO829 Melanoma Cells
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Artur Beberok , Dorota Wrześniok , Aldona Minecka , et al. ,
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Pharmacological Reports ,
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2018,
-
70(1):6-13
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, Mitochondrial Potential Assay
- Research area: Oncology
Background: Low effectiveness of anti-melanoma therapies makes it necessary to search for new drugs that could improve or replace the standard chemotherapy. Fluoroquinolones are a group of synthetic antibiotics, used in the treatment of wide range of bacterial infections. Moreover,...
View PublicationGanoderma microsporum immunomodulatory protein induces apoptosis and potentiatesmitomycin C-induced apoptosis in urinary bladder urothelial carcinoma cells
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Huang SY, Chien CC, Hseu RS, et al. ,
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J Cell Biochem ,
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2018,
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119(6):4592-4606
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, Mitochondrial Potential Assay
- Research area: Oncology
Current chemotherapy and immunotherapy treatments followed by transurethral resection for urinary bladder urothelial carcinoma (UC) usually suffer from poor prognosis and high recurrence rate. Design and modification of current formulation with the novel adjuvants are needed. A recombinant protein derived...
View PublicationImmunotoxicity of Titanium Dioxide Nanoparticles via Simultaneous Induction of Apoptosis and Multiple Toll-Like Receptors Signaling Through ROS-dependent SAPK/JNK and p38 MAPK Activation
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Madhusmita Dhupal , Jae-Min Oh , Dipti Ranjan Tripathy , et al. ,
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International Journal of Nanomedicine ,
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2018,
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13:6735-6750
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Eukaryotic Cells
- Assay: Mitochondrial Potential Assay
- Research area: Immunology
Background: Titanium dioxide nanoparticles (TiO2 NPs) represent a scientific breakthrough in the areas of biological and medicinal applications. Interaction of TiO2 NPs with components of innate immune system remains elusive. Aim: This study explored in vitro immunotoxicity of murine macrophage...
View PublicationComparison of changes in mitochondrial bioenergetics between keratinocytes in human externalauditory canal skin and cholesteatomas from normoxia to hypoxia
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Shih CP, Lee JT, Chen HK, et al. ,
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Sci Rep ,
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2018,
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8(1):125
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Mitochondrial Potential Assay
- Research area: Cell Biology
Cholesteatoma has attracted many studies seeking to uncover its nature and the pathogenesis of related diseases. However, no researchers have explored the mitochondrial bioenergetics of cholesteatoma. The aim of this study was to investigate the energy demand and differential mitochondrial...
View PublicationEvaluation of cytotoxicity, genotoxicity, and apoptosis of wastewater before and afterdisinfection with performic acid.
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Ragazzo P, Feretti D, Monarca S, et al. ,
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Water Res ,
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2017,
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116:44-52
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Hepatocytes
- Assay: DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Small Molecule Drugs
Disinfection with performic acid (PFA) represents an emerging technology in wastewater treatment. Many recent studies indicate its effectiveness and suitability as a disinfectant for different applications; several have demonstrated its reliability as an alternative to chlorine for disinfecting secondary effluents...
View PublicationThe beneficial effects of resveratrol on the steatosis and mitochondrial oxidative stress in HepG2cells
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Izdebska M, Piątkowska-Chmiel I, Korolczuk A, et al. ,
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Can J Physiol Pharmacol ,
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2017,
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95(12):1442-1453
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Hepatocytes
- Assay: Mitochondrial Potential Assay
- Research area: Cell Biology
Nonalcoholic fatty liver disease (NAFLD) is currently one of the most common chronic liver diseases, especially in developed countries. One group of substances with a potential use in the treatment of NAFLD are plant polyphenols, represented by resveratrol. The aim...
View PublicationAnti-proliferative and anti-migration effects of Polish propolis combined with Hypericum perforatum L. onglioblastoma multiforme cell line U87MG.
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Borawska MH, Naliwajko SK, Moskwa J, et al. ,
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BMC Complement Altern Med ,
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2016,
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0,921527778
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
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- Research area: Oncology
Cytotoxic hydrogen bridged ruthenium quinaldamide complexes showing induced cancer cell death by apoptosis
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RM Lord, SS Allison, K Rafferty, et al. ,
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Dalton Transactions ,
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2016,
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45(33):13196-203
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Mitochondrial Potential Assay
- Research area: Oncology
Application of an image cytometry protocol for cellular and mitochondrial phenotyping on fibroblasts from patients with inherited disorders
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Bie AS, Fernandez-Guerra P, Birkler RI, et al. ,
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Front Mol Biosci ,
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2016,
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0,170138889
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Mitochondrial Potential Assay
- Research area: Cell Biology
Adenylyl cyclase activating polypeptide reduces phosphorylation and toxicity of the polyglutamine-expanded androgen receptor in spinobulbar muscular atrophy
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Polanco MJ,, Parodi S, et al. ,
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Sci Transl Med ,
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2016,
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8(370):370ra181
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Mitochondrial Potential Assay
- Research area: Cell Biology
Application of an Image Cytometry Protocol for Cellular and Mitochondrial Phenotyping on Fibroblasts from Patients with Inherited Disorders
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Fernandez-Guerra P, Lund M, Corydon TJ, et al. ,
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JIMD Rep ,
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2016,
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27:17-26
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
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- Research area: Cell Biology
Disruption of the human COQ5-containing protein complex is associated with diminished coenzyme Q10 levels under two different conditions of mitochondrial energy deficiency.
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Yen HC, Liu YC, Kan CC, et al. ,
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Biochim Biophys Acta ,
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2016,
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pii: S0304-4165(16)30134-9
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- Assay: Mitochondrial Potential Assay
- Research area: Cell Biology
In Vitro Safety/Protection Assessment of Resveratrol and Pterostilbene in a Human HepatomaCell Line (HepG2)
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Lombardi G, Vannini S, Blasi F, et al. ,
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Nat Prod Commun ,
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2015,
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10(8):1403-8
- Instrument: NucleoCounter® NC-3000™
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Synthesis of novel C5-curcuminoid-fatty acid conjugates and mechanistic investigation of their anticancer activity
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Sanabria-Ríos DJ, Rivera-Torres Y, Rosario J, et al. ,
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Bioorg Med Chem Lett. ,
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2015,
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pii: S0960-894X(15)00281-4.
- Instrument: NucleoCounter® NC-3000™
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Polyglutamine-expanded androgen receptor interferes with TFEB to elicit autophagy defects in SBMA.
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Cortes CJ, Miranda HC, Frankowski H, et al. ,
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Nat Neurosci. ,
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2014,
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17(9):1180-9
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Imidazolium-derived ionic salts induce inhibition of cancerous cell growth through apoptosis
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Malhotra SV, Vineet Kumar, Christian Velezb and Beatriz Zayasb ,
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Med Chem Comm ,
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2014,
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5, 1404-1409
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
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- Research area: Oncology
Age influence on stromal vascular fraction cell yield obtained from human lipoaspirates.
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Vilaboa S, Navarro-Palou M, Llull R. ,
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Cytotherapy ,
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2014,
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16(8):1092-7
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
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- Research area: Cell-based Immunotherapy
A new system for quality control in hematopoietic progenitor cell units before reinfusion in autologoustransplant.
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Scerpa MC, Rossi C, Daniele N, et al. ,
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Transfusion ,
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2014,
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54(3):522-31
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Leukocytes
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- Research area: Immunology
Investigation of the cytotoxic, genotoxic, and apoptosis-inducing effects of estragole isolated from fennel(Foeniculum vulgare).
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Villarini M, Pagiotti R, Dominici L, et al. ,
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J Nat Prod ,
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2014,
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77(4):773-8
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Hepatocytes
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Cell Biology
Dynamic rearrangement of F-actin is required to maintain the antitumor effect of trichostatin A.
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Yang DH, Lee JW, Lee J, Moon EY. ,
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PLoS One ,
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2014,
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9(5):e97352
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Mitochondrial Potential Assay
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Comparison of three thiol probes for determination of apoptosis-related changes in cellular redox status.
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Skindersoe ME, Kjaerulff S. ,
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Cytometry A ,
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2014,
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85(2):179-87
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
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- Research area: Cell Biology
Mitochondrial fragmentation caused by phenanthroline promotes mitophagy
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Park SJ, Shin JH, Kim ES, et al. ,
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FEBS Lett ,
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2012,
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586(24):4303-10
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
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A cell model to study different degrees of Hsp60 deficiency in HEK293 cells
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Bie AS, Palmfeldt J, Hansen J, et al. ,
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Cell Stress Chaperones ,
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2011,
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16(6):633-40
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay, Mitochondrial Potential Assay
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Niclosamide induces mitochondria fragmentation and promotes both apoptotic and autophagic cell death
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Park SJ, Shin JH, Kang H, et al. ,
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BMB Rep ,
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2011,
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44(8):517-22
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
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A receptor tyrosine kinase inhibitor, Tyrphostin A9 induces cancer cell death through Drp1 dependent mitochondria fragmentation
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Park SJ, Park YJ, Shin JH, et al. ,
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Biochem Biophys Res Commun ,
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2011,
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408(3):465-70
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Mitochondrial Potential Assay
- Research area: Cell Biology
Proteomics Reveals that Redox Regulation Is Disrupted in Patients with Ethylmalonic Encophalopathy
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Palmfeldt J, Vang S, Stenbroen V, et al. ,
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J Proteome Res. ,
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2011,
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10(5):2389-96
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay, Mitochondrial Potential Assay
- Research area: Neurobiology