Quantitative evaluation of chromosomal rearrangements in gene-edited human stem cells by CAST-Seq

  • Turchiano G, Andrieux G, Klermund J, et al. ,

  • Cell Stem Cell ,

  • 2021,

  • 28(6):1136-1147.e5

  • Instrument: NucleoCounter® NC-250™
  • Cell Type: HSCs
  • Research area:

Genome editing has shown great promise for clinical translation but also revealed the risk of genotoxicity caused by off-target effects of programmable nucleases. Here we describe chromosomal aberrations analysis by single targeted linker-mediated PCR sequencing (CAST-Seq), a preclinical assay to...

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