Scaling up virus production without risking robustness
A rapid increase in the number of gene therapy products has led to an equally increased need for industrial production of suitable viral vectors, but current production systems lack scalability and robustness. CEVEC Pharmaceuticals CAP® GT cell lines provide a novel platform for the industrial production of viral vectors. CAP® GT cell lines are fully documented and comply with regulatory requirements. Growing in serum-free suspension culture at high densities, the cells provide a fully scalable vector production platform with reproducible transfection and high-efficiency production of viral vectors.
The precise detection of GFP transfection efficiency is a crucial step during this process, but available methods are often not sufficiently robust and are time- and labor-intensive. Thus, the GFP transfection efficiency at CEVEC is routinely determined using the NucleoCounter® NC-3000™, ensuring reproducibility for fast screening of many samples.
Optimized workflow increases lentiviral titers
To optimize lentiviral vector titers in CAP® GT cells CEVEC used a DoE approach to determine optimal cell density, and DNA and transfection reagent amounts; likewise addition of supplements were tested and the influence on viral production was analyzed. Using the optimized conditions, a 10-fold increase in lentiviral vector production was achieved. This high density culture system is scalable to any current bioreactor format.
GFP transfection efficiency with the NucleoCounter® NC-3000™
– fast and easy
Handling a lot of samples requires reliable and fast quantification of cell numbers and GFP expression. With the bench-top NucleoCounter® NC-3000™, GFP fluorescence can be easily determined. In a few minutes, the NucleoCounter® NC-3000™ reports both transfection rate and cell death. All signals are automatically quantified and results are displayed via tables, histograms and scatter plots. With the NucleoCounter® NC-3000™, you receive detailed results fast, reproducible and you can perform visual inspection of your cells.