Publications
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2023
Benzophenone-3 exposure alters composition of tumor infiltrating immune cells and increases lung seeding of 4T1 breast cancer cells
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Morin SM, Gregory KJ, Medeiros B, et al. ,
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Advances in Cancer Biology - Metastasis ,
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2023,
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7:100080
- Instrument: NucleoCounter® NC-202™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Environmental chemicals are a persistent and pervasive part of everyday life. A subset of environmental chemicals are xenoestrogens, compounds that bind to the estrogen receptor (ER) and drive estrogen-related processes. One such chemical, benzophenone-3 (BP3), is a common chemical in...
View Publication3D Microcapsules for Human Bone Marrow Derived Mesenchymal Stem Cell Biomanufacturing in a Vertical-Wheel Bioreactor
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Teryek M, Jadhav P, Bento R, Parekkadan B ,
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bioRxiv ,
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2023,
- Instrument: NucleoCounter® NC-202™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Microencapsulation of human mesenchymal stromal cells (MSCs) via electrospraying has been well documented in tissue engineering and regenerative medicine. Herein, we report the use of microencapsulation, via electrospraying, for MSC expansion using a commercially available hydrogel that is durable, optimized...
View Publication2022
Genotoxic and inflammatory effects of spruce and brown coal briquettes combustion aerosols on lung cells at the air-liquid interface
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Ihantola T, Hirvonen MR, Ihalainen M, et al. ,
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Science of The Total Environment ,
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2022,
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860;150489
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Solid fuel usage in residential heating and cooking is one of the largest sources of ambient and indoor air particulate matter, which causes adverse effects on the health of millions of peoples worldwide. Emissions from solid fuel combustion, such as...
View PublicationPotential Anticancer Agents against Melanoma Cells Based on an As-Synthesized Thiosemicarbazide Derivative
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Kozyra P, Korga-Plewko A, Karczmarzyk Z, et al. ,
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Biomolecules ,
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2022,
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12(2):151
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, Cell Vitality Assay
- Research area: Oncology
In this paper, thiosemicarbazide derivatives were synthesized as potential anticancer agents. X-ray investigations for 1-(2,4-dichlorophenoxy)acetyl-4-(2-fluorophenyl) thiosemicarbazide, 1-(2,4-dichlorophenoxy)acetyl-4-(4-metylothiophenyl)thiosemicarbazide and 1-(2,4-di chlorophenoxy)acetyl-4-(4-iodophenyl)thiosemicarbazide were carried out in order to confirm the synthesis pathways, identify their tautomeric forms, analyze the conformational preferences of molecules,...
View PublicationThe Anticancer Potential of Doxycycline and Minocycline-A Comparative Study on Amelanotic Melanoma Cell Lines
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Rok J, Rzepka Z, Kowalska J, et al. ,
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Int J Mol Sci ,
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2022,
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23(2):831
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, Cell Vitality Assay, Mitochondrial Potential Assay
- Research area: Oncology
Malignant melanoma is still a serious medical problem. Relatively high mortality, a still-growing number of newly diagnosed cases, and insufficiently effective methods of therapy necessitate melanoma research. Tetracyclines are compounds with pleiotropic pharmacological properties. Previously published studies on melanotic melanoma...
View PublicationChoice of medium affects PBMC quantification, cell size, and downstream respiratory analysis
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Christensen IB, Ribas L, Larsen S, et al. ,
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bioRxiv ,
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2022,
- Instrument: NucleoCounter® NC-3000™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
High-resolution respirometry (HRR) can assess PBMC bioenergetics, but no standardized medium for PBMC preparation and HRR analysis exist. Here, we study the effect of four different media (MiR05, PBS, RPMI, Plasmax) on quantification, size, and HRR analysis (Oxygraph-O2k) of intact...
View PublicationKR-31831 improves survival and protects hematopoietic cells and radiosensitive tissues against radiation-induced injuries in mice
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Lee JH, Yi H, Lee JH, et al. ,
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Biomed Pharmacother ,
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2022,
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146:112350
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: DNA Fragmentation Assay
- Research area: Cell Biology
This study explored the radioprotective effects and possible underlying mechanisms of KR-31831 against radiation-induced injury in a mouse model. KR-31831 (30 and 50 mg/kg) was administered to mice 24 h and 30 min before exposure to a single lethal or...
View PublicationCarnosine suppresses human colorectal cancer cell proliferation by inducing necroptosis and autophagy and reducing angiogenesis
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Hsieh SL, Li JH, Dong CD, et al. ,
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Oncol Lett ,
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2022,
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23(2):44
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay
- Research area: Oncology
Carnosine (β-alanyl-L-histidine) is found in beef and fish. The present study aimed to investigate the effects of carnosine on the cell proliferation of human colorectal cancer cells. After human colorectal cancer HCT-116 cells were treated carnosine for 72 or 96...
View PublicationInvestigation of the cytotoxicity induced by didocosahexaenoin, an omega 3 derivative, in human prostate carcinoma cell lines
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Robinson GF, Sooda KKY, Phillips RM, et al. ,
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Current Research in Pharmacology and Drug Discovery ,
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2022,
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3(100085)
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay, Mitochondrial Potential Assay
- Research area: Oncology
The aim of the present study was to investigate the cytotoxicity induced by an omega-3 derivative, didocosahexaenoin (Dido) on human prostate carcinoma cells and to compare the cytotoxicity to that of docosahexaenoic acid (DHA). Different carcinoma- and non-carcinoma cells were...
View PublicationDesign, synthesis, and biological activities of 3-((4,6-diphenylpyrimidin-2-ylamino)methylene)-2,3-dihydrochromen-4-ones
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Shin SY, Jung E, Yeo H, et al. ,
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Bioorganic Chemistry ,
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2022,
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105634
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, DNA Fragmentation Assay
- Research area: Oncology
Novel (Z)-3-((4,6-diphenylpyrimidin-2-ylamino)methylene)-2,3-dihydrochromen-4-one derivatives were designed and synthesized to find chemotherapeutic agents. Derivative 9 was selected based on its clonogenicity against cancer cells and synthetic yield for further biological experiments. It showed decreases in aurora kinase A, B, and C phosphorylation...
View PublicationExpression of membrane fusion proteins in spermatozoa and total fertilisation failure during in vitro fertilisation
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Enoiu SI, Nygaard MB, Bungum M, et al. ,
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Andrology ,
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2022,
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Sperm Cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Background: Couples increasingly experience infertility and seek help from assisted reproductive techniques to become pregnant. However, 5%-15% of the couples that are selected for in vitro fertilisation (IVF) experience a total fertilisation failure (TFF), where no zygotes develop despite oocytes...
View PublicationStreamlined and quantitative detection of chimerism using digital PCR
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Suchy FP, Nishimura T, Seki S, et al. ,
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Sci Rep ,
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2022,
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12(1):10223
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Primary Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Animal chimeras are widely used for biomedical discoveries, from developmental biology to cancer research. However, the accurate quantitation of mixed cell types in chimeric and mosaic tissues is complicated by sample preparation bias, transgenic silencing, phenotypic similarity, and low-throughput analytical...
View PublicationPhytochemical Profile and Biological Activity of the Ethanolic Extract from the Aerial Part of Crocus alatavicus Regel & Semen Growing Wildly in Southern Kazakhstan
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Allambergenova Z, Kasela M, Adamczuk G, et al. ,
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Molecules ,
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2022,
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27(11):3468
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay, Cell Vitality Assay
- Research area: Oncology
The composition of the ethanolic extract from the aerial parts of Crocus alatavicus Regel & Semen from southern Kazakhstan spontaneous flora was analyzed together with the determination of its antibacterial, antifungal, antiviral and anticancer activity. The phytochemical profile analysis by...
View PublicationChemosensitization of U-87 MG Glioblastoma Cells by Neobavaisoflavone towards Doxorubicin and Etoposide
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Maszczyk M, Banach K, Karkoszka M, et al. ,
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Int J Mol Sci ,
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2022,
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23(10):5621
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, Mitochondrial Potential Assay
- Research area: Oncology
Glioblastoma (GB) is the most common type of glioma, which is distinguished by high mortality. Due to the rapid progression of the tumor and drug resistance, the treatment is often ineffective. The development of novel therapies in a big part...
View PublicationCopper oxide nanoparticles trigger macrophage cell death with misfolding of Cu/Zn superoxide dismutase 1 (SOD1)
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Gupta G, Cappellini F, Farcal L, et al. ,
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Part Fibre Toxicol ,
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2022,
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19(1):33
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Immune Cells
- Research area: Cell Biology
Background: Copper oxide (CuO) nanoparticles (NPs) are known to trigger cytotoxicity in a variety of cell models, but the mechanism of cell death remains unknown. Here we addressed the mechanism of cytotoxicity in macrophages exposed to CuO NPs versus copper...
View PublicationEfficacy of mesenchymal stem cell-delivery using perpendicular multi-needle injections to the skin: Evaluation of post-ejection cellular health and dermal delivery
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Rangatchew F, Rasmussen BS, Svalgaard JD, et al. ,
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Burns ,
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2022,
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S0305-4179(22)00098-5
- Instrument: NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
Aim: Mesenchymal stem cell (MSC)-therapy is increasingly being evaluated in clinical trials. Dermal delivery is not only time consuming but also unreliable, potentially hampering the therapeutic result. Therefore, qualification of cell delivery protocols is essential. This study evaluated a clinically...
View PublicationVariations in the Anticancer Activity of Free and Bound Phenolics of Finger Millet (Eleusine coracana (L) Gaertn; Variety KMR-301) Seeds
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Kuruburu MG, Bovilla VR, Naaz R, et al. ,
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Phytomedicine Plus ,
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2022,
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2(2):100276
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, DNA Fragmentation Assay
- Research area: Oncology
Background Finger millet (FM, Eleusine coracana (L) Gaertn; also known as “ragi” in Kannada language) is one of the widely used millets especially in south India and parts of Africa. FM is consumed in various forms that include porridge, mudde...
View PublicationA Comparative Study on the Adipogenic Differentiation of Mesenchymal Stem/Stromal Cells in 2D and 3D Culture
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Wolff A, Frank M, Staehlke S, Peters K ,
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Cells ,
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2022,
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11(8):1313
- Instrument: NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Mesenchymal stem/stromal cells (MSC) are capable of renewing the progenitor cell fraction or differentiating in a tissue-specific manner. Adipogenic differentiation of adipose-tissue-derived MSC (adMSC) is important in various pathological processes. Adipocytes and their progenitors are metabolically active and secrete molecules...
View PublicationKrüppel-like factor 2 controls IgA plasma cell compartmentalization and IgA responses
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Wittner J, Schulz SR, Steinmetz TD, et al. ,
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Mucosal Immunol ,
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2022,
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15(4):668-682
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Krüppel-like factor 2 (KLF2) is a potent regulator of lymphocyte differentiation, activation and migration. However, its functional role in adaptive and humoral immunity remains elusive. Therefore, by using mice with a B cell-specific deletion of KLF2, we investigated plasma cell...
View PublicationBiomimetic Strain-Stiffening in Chitosan Self-Healing Hydrogels
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Liu Y, Lin SH, Chuang WT, et al. ,
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Healing Hydrogels ,
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2022,
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14(14):16032-16046
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Hepatocytes
- Assay: Cell Vitality Assay
- Research area: Cell Biology
The strain-stiffening and self-healing capabilities of biological tissues enable them to preserve the structures and functions from deformation and damage. However, biodegradable hydrogel materials with both of these biomimetic characteristics have not been explored. Here, a series of strain-stiffened, self-healing...
View PublicationSerum Testosterone Levels in 3-Month-Old Boys Predict Their Semen Quality as Young Adults
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Scheutz Henriksen L, Holm Petersen J, Skakkebæk NE, et al. ,
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J Clin Endocrinol Metab ,
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2022,
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107(7):1965-1975
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Sperm Cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Context: It remains unknown how the postnatal activation of the hypothalamic-pituitary-gonadal axis in infancy, also known as “minipuberty”, relates to adult testis function. Objective: To investigate how markers of reproductive function in 3-month-old boys correlate with adult reproductive health parameters....
View PublicationLong Noncoding RNA LOC550643 Acts as an Oncogene in the Growth Regulation of Colorectal Cancer Cells
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Wu HF, Lu TJ, Lo YH, et al. ,
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Cells ,
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2022,
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11(7):1065
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Oncology
Long noncoding RNAs play a key role in the progression of colorectal cancer (CRC). However, the role and mechanism of LOC550643 in CRC cell growth and metastasis remain largely unknown. In this study, we assessed the clinical impacts of LOC550643...
View PublicationTransduction of HEK293 Cells with BacMam Baculovirus Is an Efficient System for the Production of HIV-1 Virus-like Particles
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Puente-Massaguer E, Cajamarca-Berrezueta B, Volart A, et al. ,
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Viruses ,
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2022,
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14(3):636
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Virus Production
Gag virus-like particles (VLPs) are promising vaccine candidates against infectious diseases. VLPs are generally produced using the insect cell/baculovirus expression vector system (BEVS), or in mammalian cells by plasmid DNA transient gene expression (TGE). However, VLPs produced with the insect...
View PublicationSelection of O-negative induced pluripotent stem cell clones for high-density red blood cell production in a scalable perfusion bioreactor system
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Yu S, Vassilev S, Lim ZR, et al. ,
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Cell Prolif ,
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2022,
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e13218
- Instrument: NucleoCounter® NC-3000™
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Objectives: Large-scale generation of universal red blood cells (RBCs) from O-negative (O-ve) human induced pluripotent stem cells (hiPSCs) holds the potential to alleviate worldwide shortages of blood and provide a safe and secure year-round supply. Mature RBCs and reticulocytes, the...
View PublicationThe non-ELR CXC chemokine encoded by human cytomegalovirus UL146 genotype 5 contains a C-terminal β-hairpin and induces neutrophil migration as a selective CXCR2 agonist
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Berg C, Wedemeyer MJ, Melynis M, et al. ,
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PLoS Pathog ,
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2022,
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18(3):e1010355
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Human cytomegalovirus (HCMV) is a major pathogen in immunocompromised patients. The UL146 gene exists as 14 diverse genotypes among clinical isolates, which encode 14 different CXC chemokines. One genotype (vCXCL1GT1) is a known agonist for CXCR1 and CXCR2, while two...
View PublicationIn vitro investigation of endocrine disrupting effects of pesticides on Ca2+-signaling in human sperm cells through actions on the sperm-specific and steroid-activated CatSper Ca2+-channel
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Birch MR, Johansen M, Skakkebæk NE, et al. ,
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Environment International ,
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2022,
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107399
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Sperm Cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
Background Ca2+-signaling controls sperm cell functions necessary for successful fertilization. Multiple endocrine disrupting chemicals have been found to interfere with normal Ca2+-signaling in human sperm cells through an activation of the sperm-specific CatSper Ca2+-channel, which is vital for normal male...
View PublicationAnti-Obesity Effect of Nostoc commune Ethanol Extract In Vitro and In Vivo
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Tsai SC, Huang YW, Wu CC, et al. ,
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Nutrients ,
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2022,
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14(5):968
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Primary Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Nostoc commune is an edible terrestrial blue-green alga. It has shown many beneficial effects on human health. This study aimed to investigate the phytochemical assay of N. commune ethanol extract (NEE) and its anti-obesity effects. The effect of a high-calorie...
View PublicationMLL-AF4 and a murinized pSer-variant thereof are turning on the nucleolar stress pathway
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Siemund AL, Hanewald T, Kowarz E, Marschalek R ,
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Cell Biosci ,
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2022,
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12(1):47
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Background: Recent pathomolecular studies on the MLL-AF4 fusion protein revealed that the murinized version of MLL-AF4, the MLL-Af4 fusion protein, was able to induce leukemia when expressed in murine or human hematopoietic stem/progenitor cells (Lin et al. in Cancer Cell...
View PublicationSize-based isolation and detection of renal carcinoma cells from whole blood
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De Alwis R, Hansson J, Lindgren D, et al. ,
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Mol Clin Oncol ,
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2022,
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16(5):101
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Primary Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Renal cell carcinoma (RCC) is a tumour type with an indolent growth pattern and rather vague symptoms. The present study developed a platform for liquid biopsy of RCC based upon the isolation of circulating tumour cells (CTCs). Founded on the...
View PublicationResveratrol butyrate esters inhibit lipid biosynthesis in 3T3-L1 cells by AMP-activated protein kinase phosphorylation
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Shih MK, Hsieh SL, Huang YW, et al. ,
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Journal of Food Science and Technology ,
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2022,
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Epithelial Cells
- Assay: Cell Cycle Assay, Cell Vitality Assay
- Research area: Cell Biology
Resveratrol butyrate esters (RBEs), which are novel resveratrol-synthesized derivatives, exhibit increased biological activity. This study elucidated the effect of RBEs on fat metabolism and their anti-obesity characteristics. Their molecular mechanism was investigated in the 3T3-L1 murine preadipocyte cells and adipocytes....
View PublicationNSAIDs Induce Proline Dehydrogenase/Proline Oxidase-Dependent and Independent Apoptosis in MCF7 Breast Cancer Cells
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Kazberuk A, Chalecka M, Palka J, et al. ,
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Int J Mol Sci ,
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2022,
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23(7):3813
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay
- Research area: Oncology
Non-steroidal anti-inflammatory drugs (NSAIDs) are considered in cancer therapy for their inhibitory effect on cyclooxygenase-2 (COX-2), which is overexpressed in most cancers. However, we found that NSAIDs as ligands of peroxisome proliferator-activated receptor-γ (PPARγ)-induced apoptosis independent of the COX-2 inhibition,...
View PublicationEffects of hydrophilic fullerene nanoarchitectured structures on the behaviour of neural stem cells
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Wong CW, Tsai KC, Shrestha LK, et al. ,
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Nanoscale ,
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2022,
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Neural Cells
- Assay: Cell Vitality Assay
- Research area: Stem Cell Research
The interaction between nanoarchitectonic fullerenes and cells is essential for their applications in the biological field. Herein we reported the preparation and investigation of the function of different types of water-dispersible self-assembled fullerenes. The hydrophobic self-assembled fullerenes were either surface-modified...
View PublicationNovel human-derived EML4-ALK fusion cell lines identify ribonucleotide reductase RRM2 as a target of activated ALK in NSCLC
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Bokhari AA, Lai WY, Le AT, et al. ,
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Lung Cancer ,
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2022,
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171:103-114
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Introduction Echinoderm microtubule-associated protein-like 4 (EML4)-Anaplastic Lymphoma Kinase (ALK) rearrangements occur in 3% to 7% of lung adenocarcinomas and are targets for treatment with tyrosine kinase inhibitors (TKIs). Here we have developed three novel EML4-ALK-positive patient–derived Non-Small-Cell-Lung-Cancer (NSCLC) cancer cell...
View PublicationPOLQ suppresses genome instability and alterations in DNA repeat tract lengths
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Liddiard K, Aston-Evans AN, Cleal K, et al. ,
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NAR Cancer ,
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2022,
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4(3):zcac020
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
DNA polymerase theta (POLQ) is a principal component of the alternative non-homologous end-joining (ANHEJ) DNA repair pathway that ligates DNA double-strand breaks. Utilizing independent models of POLQ insufficiency during telomere-driven crisis, we found that POLQ – /- cells are resistant...
View PublicationA tubulin binding molecule drives differentiation of acute myeloid leukemia cells
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Jackson TR, Vuorinen A, Josa-Culleré L, et al. ,
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ISCIENCE ,
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2022,
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Despite much progress in developing better drugs, many patients with Acute Myeloid Leukaemia (AML) still die within a year of diagnosis. This is partly because it is difficult to identify therapeutic targets that are effective across multiple AML subtypes. One...
View PublicationCDI Exerts Anti-Tumor Effects by Blocking the FoxM1-DNA Interaction
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Jang WD, Lee MY, Mun J, et al. ,
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Biomedicines ,
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2022,
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10(7):1671
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Epithelial Cells
- Assay: Annexin V Assay
- Research area: Oncology
The Forkhead box protein M1 (FoxM1) is an appealing target for anti-cancer therapeutics as this cell proliferation-associated transcription factor is overexpressed in most human cancers. FoxM1 is involved in tumor invasion, angiogenesis, and metastasis. To discover novel inhibitors that disrupt...
View PublicationFilamentous nuclear actin regulation of PML-NBs during the DNA damage response is deregulated by prelamin A
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Cobb A, De Silva S, Hayward R, et al. ,
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Cell death & differentiation ,
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2022,
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Vitality Assay
- Research area: Cell Biology
Nuclear actin participates in a continuously expanding list of core processes within eukaryotic nuclei including the maintenance of genomic integrity. In response to DNA damage, nuclear actin polymerises into filaments that are involved in the repair of damaged DNA through...
View PublicationNox4 promotes endothelial differentiation through chromatin remodeling
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Hahner F, Moll F, Warwick T, et al. ,
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Redox Biol ,
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2022,
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55:102381
- Instrument: NucleoCounter® NC-3000™
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Rationale: Nox4 is a constitutively active NADPH oxidase that constantly produces low levels of H2O2. Thereby, Nox4 contributes to cell homeostasis and long-term processes, such as differentiation. The high expression of Nox4 seen in endothelial cells contrasts with the low...
View PublicationEquid Alphaherpesvirus 1 (EHV-1) Influences Morphology and Function of Neuronal Mitochondria In Vitro
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Chodkowski M, Słońska A, Gregorczyk-Zboroch K, et al. ,
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Pathogens ,
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2022,
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11(8):876
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Neural Cells
- Assay: Mitochondrial Potential Assay
- Research area: Neurobiology
Mitochondria are key cellular organelles responsible for many essential functions, including ATP production, ion homeostasis and apoptosis induction. Recent studies indicate their significant role during viral infection. In the present study, we examined the effects of equine herpesvirus type 1...
View PublicationSubstrate viscosity impairs temozolomide-mediated inhibition of glioblastoma cells’ growth
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Cieśluk M, Piktel E, Wnorowska U, et al. ,
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Biochim Biophys Acta Mol Basis Dis ,
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2022,
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166513
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: DNA Fragmentation Assay
- Research area: Oncology
Background: The mechanical state of the extracellular environment of the brain cells considerably affects their phenotype during the development of central nervous system (CNS) pathologies, and when the cells respond to drugs. The reports on the evaluation of the viscoelastic...
View PublicationPreparation and growth factor characterization of cord blood-derived plasma, serum, growth factor-rich plasma and induced serum
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Rhéaume ME, Perreault J, Fournier D, Trépanier P ,
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Cytokine ,
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2022,
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149:155756
- Instrument: NucleoCounter® NC-200™
- Cell Type: Whole Blood
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Cultured meat is an emergent technology with the potential for significant environmental and animal welfare benefits. Accurate mimicry of traditional meat requires fat tissue; a key contributor to both the flavour and texture of meat. Here, we show that fibro-adipogenic...
View PublicationMuscle-derived fibro-adipogenic progenitor cells for production of cultured bovine adipose tissue
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Dohmen RGJ, Hubalek S, Melke J, et al. ,
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NPJ Sci Food ,
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2022,
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6(1):6
- Instrument: NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cultured Meat
Background and aims: Autologous keratinocyte sheets constitute an important component of the burn wound treatment toolbox available to a surgeon and can be considered a life-saving procedure for patients with severe burns over 50% of their total body surface area....
View PublicationOptimizing large-scale autologous human keratinocyte sheets for major burns—Toward an animal-free production and a more accessible clinical application
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Frese L, Darwiche SE, Gunning ME, et al. ,
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Health Sci Rep ,
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2022,
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5(1):e449
- Instrument: NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Nickel oxide nanoparticles (NiO NPs) are highly redox active nanoparticles. They can cause acute and chronic inflammation in rat lungs. Unlike the gut microbiome, the association between the lung microbiome’s role and pulmonary inflammatory response to inhaled nanoparticles remains largely...
View PublicationExposure to Nickel Oxide Nanoparticles Induces Acute and Chronic Inflammatory Responses in Rat Lungs and Perturbs the Lung Microbiome
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Jeong MJ, Jeon S, Yu HS, et al. ,
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Int J Environ Res Public Health ,
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2022,
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19(1):522
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Three-dimensional (3D)-bioprinted lipoaspirate-derived adipose tissue (LAT) is a potential alternative to lipo-injection for correcting soft-tissue defects. This study investigated the long-term in vivo survival of 3D-bioprinted LAT and its proteomic signature and cellular composition. We performed proteomic and multicolour flow...
View PublicationLong-term in vivo survival of 3D-bioprinted human lipoaspirate-derived adipose tissue: proteomic signature and cellular content
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Säljö K, Apelgren P, Stridh Orrhult L, et al. ,
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Adipocyte ,
-
2022,
-
11(1):34-46
- Instrument: NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Chimeric antigen receptor T (CAR-T) cell therapy has led to a paradigm change in cancer treatment. To allow widespread use of this therapy, it is important to reduce the costs associated with cells manufacturing whilst ensuring a high-quality production. Online...
View PublicationAn impedimetric immunosensor for the selective detection of CD34+ T-cells in human serum
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Díaz-Fernández A, Bernalte E, Fernández-Ramos C, et al. ,
-
Sensors and Actuators B: Chemical ,
-
2022,
-
356(131306)
- Instrument: NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Cell therapy using multipotential stromal cells (MSCs) is being used in a variety of clinical settings to induce tissue regeneration. Promising results have also been achieved in the therapy of osteoarthritis. MSCs have been demonstrated to be safe (Borakati et...
View PublicationInfiltration of the Hoffa’s fat pad in patients with osteoarthritis of the knee-Results after one year of follow-up
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Labarre KW, Zimmermann G ,
-
Bone Reports ,
-
2022,
-
101168
- Instrument: NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Erectile dysfunction is a common complication associated with type 2 diabetes mellitus (T2DM) and after prostatectomy in relation to cancer. The regenerative effect of cultured adipose-derived stem cells (ASCs) for ED therapy has been documented in multiple preclinical trials as...
View PublicationAdipose-Derived Stem Cells from Type 2 Diabetic Rats Retain Positive Effects in a Rat Model of Erectile Dysfunction
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Quaade ML, Dhumale P, Steffensen SGC, et al. ,
-
International Journal of Molecular Sciences ,
-
2022,
-
23(3):1692
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
The promise of human induced pluripotent stem cells (iPSCs) lies in their ability to serve as a starting material for autologous, or patient-specific, stem cell-based therapies. Since the first publications describing the generation of iPSCs from human tissue in 2007,...
View PublicationValidation of automated fluorescent-based technology for measuring total nucleated cell viability of hematopoietic progenitor cell products
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Kanan A. Patel,Nita Patel,Grace H. Kim, et al. ,
-
Transfusion ,
-
2022,
- Instrument: NucleoCounter® NC-200™
- Cell Type: HSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Background A reliable rapid method for measuring total nucleated cell (TNC) viability is essential for cell-based products manufacturing. The trypan blue (TB) exclusion method, commonly used to measure TNC viability of hematopoietic progenitor cell (HPC) products, is a subjective assay,...
View PublicationNovel CD19 chimeric antigen receptor T cells manufactured next-day for acute lymphoblastic leukemia
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Zhang C, He J, Liu L, et al. ,
-
Blood Cancer J ,
-
2022,
-
12(6):96
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Chimeric antigen receptor-engineered T (CAR-T) cells have shown promising efficacy in patients with relapsed/refractory B cell acute lymphoblastic leukemia (R/R B-ALL). However, challenges remain including long manufacturing processes that need to be overcome. We presented the CD19-targeting CAR-T cell product...
View PublicationEnhancing CHO cell productivity through a dual selection system using Aspg and Gs in glutamine free medium
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Ha T, Òdena A, Karottki KJlC, et al. ,
-
Authorea ,
-
2022,
- Instrument: NucleoCounter® NC-200™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
The dominant method for generating Chinese hamster ovary (CHO) cell lines that produce high titers of biotherapeutic proteins utilizes selectable markers such as dihydrofolate reductase (Dhfr) or glutamine synthetase (Gs), alongside inhibitory compounds like methotrexate (MTX) or methionine sulfoximine (MSX),...
View PublicationYAP activates pancreatic stellate cells and enhances pancreatic fibrosis
-
Spanehl L, Revskij D, Bannert K, et al. ,
-
Hepatobiliary & Pancreatic Diseases International ,
-
2022,
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background Pancreatic stellate cells (PSCs) foster the progression of pancreatic adenocarcinoma and chronic pancreatitis (CP) by producing a dense fibrotic stroma. However, the incomplete knowledge of PSCs biology hampers the exploration of antifibrotic therapies. Here, we explored the role of...
View PublicationFat Grafting With Expanded Adipose-derived Stromal Cells for Breast Augmentation: A Randomized Controlled Trial
-
Vester-Glowinski PV, Herly M, Ørholt M, et al. ,
-
Aesthet Surg J ,
-
2022,
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Background The main challenge with fat grafting is that a varying part of the graft is lost to post-surgery resorption. Previous studies suggest that adipose-derived stromal cells (ASCs) can improve the volume retention of fat grafts but there is a...
View PublicationA Platform of Patient-Derived Microtumors Identifies Individual Treatment Responses and Therapeutic Vulnerabilities in Ovarian Cancer
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Anderle N, Koch A, Gierke B, et al. ,
-
Cancers ,
-
2022,
-
14(12):2895
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
In light of the frequent development of therapeutic resistance in cancer treatment, there is a strong need for personalized model systems representing patient tumor heterogeneity, while enabling parallel drug testing and identification of appropriate treatment responses in individual patients. Using...
View PublicationStandard toxicity study of clinical-grade allogeneic human bone marrow-derived clonal mesenchymal stromal cells
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Tayebi B, Babaahmadi M, Pakzad M, et al. ,
-
Stem Cell Res Ther ,
-
2022,
-
13(1):213
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Introduction: Mesenchymal stromal cells (MSCs) have opened a new window to treat inflammatory and non-inflammatory diseases. Nonetheless, their clinical applications require rigorous control and monitoring procedures to ensure full compliance with the principles of good manufacturing practice (GMP). Various evaluations...
View PublicationTreatment of Abdominal Aortic Aneurysm Utilizing Adipose-Derived Mesenchymal Stem Cells in a Porcine Model
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Zilberman B, Kooragayala K, Lou J, et al. ,
-
J Surg Res ,
-
2022,
-
278:247-256
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cardiology
Introduction: The current treatment paradigm of abdominal aortic aneurysms (AAA) focuses on observing patients until their disease reaches certain thresholds for intervention, with no preceding treatment available. There is an opportunity to develop novel therapies to prevent further aneurysmal growth...
View PublicationScalable expansion of iPSC and their derivatives across multiple lineages
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Kwok CK, Sébastien I, Hariharan K, et al. ,
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Reprod Toxicol ,
-
2022,
-
112:23-35
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Induced pluripotent stem cell (iPSC) technology enabled the production of pluripotent stem cell lines from somatic cells from a range of known genetic backgrounds. Their ability to differentiate and generate a wide variety of cell types has resulted in their...
View PublicationLarge-scale engineering of hiPSC-derived nephron sheets and cryopreservation of their progenitors
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Wiersma LE, Avramut MC, Lievers E, et al. ,
-
Stem Cell Res Ther ,
-
2022,
-
13(1):208
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Background: The generation of human induced pluripotent stem cells (hiPSCs) has opened a world of opportunities for stem cell-based therapies in regenerative medicine. Currently, several human kidney organoid protocols are available that generate organoids containing kidney structures. However, these kidney...
View PublicationCD206+ tumor-associated macrophages cross-present tumor antigen and drive antitumor immunity
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Modak M, Mattes AK, Reiss D, et al. ,
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JCI Insight ,
-
2022,
-
7(11):e155022
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
In many solid cancers, tumor-associated macrophages (TAM) represent the predominant myeloid cell population. Antigen (Ag) cross-presentation leading to tumor Ag-directed cytotoxic CD8+ T cell responses is crucial for antitumor immunity. However, the role of recruited monocyte-derived macrophages, including TAM, as...
View PublicationOptimization of a PDMS-Based Cell Culture Substrate for High-Density Human-Induced Pluripotent Stem Cell Adhesion and Long-Term Differentiation into Cardiomyocytes under a Xeno-Free Condition
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Etezadi F, Le MNT, Shahsavarani H, et al. ,
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ACS Biomater Sci Eng ,
-
2022,
-
8(5):2040-2052
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Despite the numerous advantages of PDMS-based substrates in various biomedical applications, they are limited by their highly hydrophobic surface that does not optimally interact with cells for attachment and growth. Hence, the lack of lengthy and straightforward procedures for high-density...
View PublicationExposure of human immune cells, to the antiretrovirals efavirenz and lopinavir, leads to lower glucose uptake and altered bioenergetic cell profiles through interactions with SLC2A1
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Heaton BJ, Jensen RL, Line J, et al. ,
-
Biomed Pharmacother ,
-
2022,
-
150:112999
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
SLC2A1 mediates glucose cellular uptake; key to appropriate immune function. Our previous work has shown efavirenz and lopinavir exposure inhibits T cell and macrophage responses, to known agonists, likely via interactions with glucose transporters. Using human cell lines as a...
View PublicationStromal Vascular Fraction and Mesenchymal Stem Cells from Human Adipose Tissue: A Comparison of Immune Modulation and Angiogenic Potential
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Tran TDX, Pham VQ, Tran NN, et al. ,
-
Adv Exp Med Biol ,
-
2022,
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Introduction: In recent years, both stromal vascular fraction (SVF) from adipose tissue and mesenchymal stem cells (MSC) from adipose tissues were extensively used in both preclinical and clinical treatment for various diseases. Some studies reported differences in treatment efficacy between...
View PublicationQbD-guided pharmaceutical development of Pembrolizumab biosimilar candidate PSG-024 propelled to industry meeting primary requirements of comparability to Keytruda®
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Jaffar-Aghaei M, Khanipour F, Maghsoudi A, et al. ,
-
Eur J Pharm Sci ,
-
2022,
-
173:106171
- Instrument: NucleoCounter® NC-200™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
Pharmaceutical development of biosimilars is primarily focused on meeting the regulatory requirements for analytical comparability of the product’s critical quality attributes (CQAs), concerning safety and efficacy, to those of the originator drug of interest. To this end, the early adoption...
View PublicationAnti-CTLA-4 and anti-PD-1 immunotherapies repress tumor progression in preclinical breast and colon model with independent regulatory T cells response
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Rupp T, Genest L, Babin D, et al. ,
-
Transl Oncol ,
-
2022,
-
20:101405
- Instrument: NucleoCounter® NC-200™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
The recent development of immunotherapy represents a significant breakthrough in cancer therapy. Several immunotherapies provide robust efficacy gains in a wide variety of cancers. However, in some patients the immune checkpoint blockade remains ineffective due to poor therapeutic response and...
View PublicationInhibition of lung microbiota-derived proapoptotic peptides ameliorates acute exacerbation of pulmonary fibrosis
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D'Alessandro-Gabazza CN, Yasuma T, Kobayashi T, et al. ,
-
Nat Commun ,
-
2022,
-
13(1):1558
- Instrument: NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
Idiopathic pulmonary fibrosis is an incurable disease of unknown etiology. Acute exacerbation of idiopathic pulmonary fibrosis is associated with high mortality. Excessive apoptosis of lung epithelial cells occurs in pulmonary fibrosis acute exacerbation. We recently identified corisin, a proapoptotic peptide...
View PublicationDesign and biofabrication of a leaf-inspired vascularized cell-delivery device
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Sämfors S, Niemi EM, Oskarsdotter K, et al. ,
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Bioprinting ,
-
2022,
-
26:e00199
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
We designed and biofabricated a channeled construct as a possible cell-delivery device that can be endothelialized to overcome size limitations due to oxygen diffusion. The channeled device mimicking a leaf was designed using computer-aided design software, with fluid flow through...
View PublicationExtracorporeal immune cell therapy of sepsis: ex vivo results
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Klinkmann G, Wild T, Heskamp B, et al. ,
-
Intensive Care Med Exp ,
-
2022,
-
10(1):26
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Background: Immune cell dysfunction plays a central role in sepsis-associated immune paralysis. The transfusion of healthy donor immune cells, i.e., granulocyte concentrates (GC) potentially induces tissue damage via local effects of neutrophils. Initial clinical trials using standard donor GC in...
View PublicationImmuno-reactive cancer organoid model to assess effects of the microbiome on cancer immunotherapy
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Shelkey E, Oommen D, Stirling ER, et al. ,
-
Sci Rep ,
-
2022,
-
12(1):9983
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Immune checkpoint blockade (ICB) therapy has demonstrated good efficacy in many cancer types. In cancers such as non-resectable advanced or metastatic triple-negative breast cancer (TNBC), it has recently been approved as a promising treatment. However, clinical data shows overall response...
View PublicationAssociation of heat shock protein 8 with atopic march in a murine experimental model
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Jeong KT, Do JH, Lee SH, et al. ,
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PeerJ ,
-
2022,
-
10:e13247
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Atopic march (AM), a unique characteristic of allergic diseases, refers to the sequential progression of atopic dermatitis (AD) in infants to allergic asthma and allergic rhinitis in children and young adults, respectively. Although there are several studies on AM,...
View PublicationFractal Dimension and Texture Analysis in the Assessment of Experimental Laser-Induced Periodic Surface Structures (LIPSS) Dental Implant Surface—In Vitro Study Preliminary Report
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Hadzik J, Kubasiewicz-Ross P, Simka W, et al. ,
-
Materials (Basel) ,
-
2022,
-
15(8):2713
- Instrument: NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Laser-induced periodic surface structures (LIPSS) are the sub-wavelength periodic nanostructures generated by the femtosecond laser. Implant topography and its nanostructural changes can be important for biomedical applications. In order to compare the surface topography of different implants, appropriate mathematical and...
View PublicationIn vitro culture at 39 °C during hepatic maturation of human ES cells facilitates hepatocyte-like cell functions
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Imamura S, Yoshimoto K, Terada S, et al. ,
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Sci Rep ,
-
2022,
-
12(1):5155
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Hepatocyte-like cells derived from human pluripotent stem cells (hPSC-HLCs) offer an alternative to primary hepatocytes commonly used for drug screenings and toxicological tests. However, these cells do not have hepatic functions comparable to those of hepatocytes in vivo due to...
View PublicationMicrowell bag culture for large-scale production of homogeneous islet-like clusters
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Suenaga R, Konagaya S, Yamaura J, et al. ,
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Sci Rep ,
-
2022,
-
12(1):5221
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Pluripotent stem-cell derived cells can be used for type I diabetes treatment, but we require at least 105-106 islet-like clusters per patient. Although thousands of uniform cell clusters can be produced using a conventional microwell plate, numerous obstacles need to...
View PublicationH3K9 dimethylation safeguards cancer cells against activation of the interferon pathway
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Hansen AM, Ge Y, Schuster MB, et al. ,
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Sci Adv ,
-
2022,
-
8(11):eabf8627
- Instrument: NucleoCounter® NC-200™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Activation of interferon genes constitutes an important anticancer pathway able to restrict proliferation of cancer cells. Here, we demonstrate that the H3K9me3 histone methyltransferase (HMT) suppressor of variegation 3-9 homolog 1 (SUV39H1) is required for the proliferation of acute myeloid...
View PublicationEx Vivo Hydrostatic Pressure Loading of Atrial Tissues Activates Profibrotic Transcription via TGF-β Signal Pathway
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Zhang X, Yassouf Y, Huang K, et al. ,
-
Int Heart J. ,
-
2022,
-
63(2):367-374
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Excessive mechanical stress causes fibrosis-related atrial arrhythmia. Herein, we tried to investigate the mechanism of atrial fibrogenesis in response to mechanical stress by ex vivo approach. We collected atrial tissues from mice and then cultured them as “explants” under atmospheric...
View PublicationLongitudinal two-dimensional gas chromatography mass spectrometry as a non-destructive at-line monitoring tool during cell manufacturing identifies volatile features correlative to cell product quality
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Jimenez AC, Heist CA, Navaei M, et al. ,
-
Cytotherapy ,
-
2022,
-
S1465-3249(22)00681-8
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background aims: Cell therapies have emerged as a potentially transformative therapeutic modality in many chronic and incurable diseases. However, inherent donor and patient variabilities, complex manufacturing processes, lack of well-defined critical quality attributes and unavailability of in-line or at-line process...
View PublicationEnhancing Adoptive Cell Therapy by T Cell Loading of SHP2 Inhibitor Nanocrystals before Infusion
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Li X, Halldórsdóttir HR, Weller S, et al. ,
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Nano ,
-
2022,
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Whereas adoptive T cell therapy has been extensively studied for cancer treatment, the response is still limited primarily due to immune dysfunction related to poor cell engraftment, tumor infiltration and engagement, and lack of a target. In addition, the modification...
View PublicationParenchymal and Inflammatory Cell Responses to Single and Repeated Ozone Exposure in Healthy and Surfactant Protein-C Mutant Lung
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Nguyen J, Deering-Rice CE, Armstrong BS, et al. ,
-
Toxicol Sci ,
-
2022,
-
kfac074
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Mutations in the alveolar epithelial-specific gene encoding for surfactant protein C (SP-C) are linked to pulmonary disease. Ozone (O3) is a ubiquitous pollutant known to exacerbate stress through oxidative injury and inflammation. To comprehend the structural, functional, and immunological impact...
View PublicationClinical and laboratory findings following transplantation of allogeneic adipose-derived mesenchymal stromal cells in knee osteoarthritis, a brief report
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Sadri B, Tamimi A, Nouraein S, et al. ,
-
Connect Tissue Res ,
-
2022,
-
01/Dec
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
Background: Mesenchymal stromal cells (MSCs) injection has been proposed as an innovative treatment for knee osteoarthritis (KOA). Since, allogeneic MSCs can be available as off-the-shelf products, they are preferable in regenerative medicine. Among different sources for MSCs, adipose-derived MSCs (AD-MSCs)...
View PublicationEmulsion-templated microparticles with tunable stiffness and topology: Applications as edible microcarriers for cultured meat
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Norris SCP, Kawecki NS, Davis AR, et al. ,
-
Biomaterials ,
-
2022,
-
287:121669
- Instrument: NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cultured Meat
Cultured meat has potential to diversify methods for protein production, but innovations in production efficiency will be required to make cultured meat a feasible protein alternative. Microcarriers provide a strategy to culture sufficient volumes of adherent cells in a bioreactor...
View PublicationSingle-cell transcriptome and cell type-specific molecular pathways of human non-alcoholic steatohepatitis
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Fred RG, Steen Pedersen J, Thompson JJ, et al. ,
-
Sci Rep ,
-
2022,
-
12(1):13484
- Instrument: NucleoCounter® NC-200™
- Cell Type: Hepatocytes
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The aim of this study is to characterize cell type-specific transcriptional signatures in non-alcoholic steatohepatitis (NASH) to improve our understanding of the disease. We performed single-cell RNA sequencing on liver biopsies from 10 patients with NASH. We applied weighted gene...
View PublicationUpscaling human mesenchymal stromal cell production in a novel vertical-wheel bioreactor enhances extracellular vesicle secretion and cargo profile
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Jeske R, Liu C, Duke L, et al. ,
-
Bioactive Materials ,
-
2022,
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Human mesenchymal stromal cells (hMSCs) are mechanically sensitive undergoing phenotypic alterations when subjected to shear stress, cell aggregation, and substrate changes encountered in 3D dynamic bioreactor cultures. However, little is known about how bioreactor microenvironment affects the secretion and cargo...
View PublicationImproving cell viability using counterflow centrifugal elutriation
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Li A, Barabadi M, McDonald H, et al. ,
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Cytotherapy ,
-
2022,
-
24(6):650-658
- Instrument: NucleoCounter® NC-202™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Cell viability is an important release criterion in the manufacturing of cell therapy products. Low cell viability can have significant impact on product quality and manufacturing efficiency. Counterflow centrifugation technology has been applied to the manufacturing of cell therapy...
View PublicationMapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq
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Replogle JM, Saunders RA, Pogson AN, et al. ,
-
Cell ,
-
2022,
-
185(14):2559-2575.e28
- Instrument: NucleoCounter® NC-202™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
A central goal of genetics is to define the relationships between genotypes and phenotypes. High-content phenotypic screens such as Perturb-seq (CRISPR-based screens with single-cell RNA-sequencing readouts) enable massively parallel functional genomic mapping but, to date, have been used at limited...
View PublicationRapid Antibody Glycoengineering in CHO Cells Via RNA Interference and CGE-LIF N-Glycomics
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Kotidis P, Marbiah M, Donini R, et al. ,
-
Methods Mol Biol ,
-
2022,
-
2370:147-167
- Instrument: NucleoCounter® NC-250™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Graft-contaminating tumor cells correlate with inferior outcome in high-risk neuroblastoma patients undergoing hematopoietic stem cell transplantation and can contribute to relapse. Motivated by the potential therapeutic benefit of tumor cell removal as well as the high prognostic and diagnostic...
View PublicationCharacterization of the Antibacterial Activity of an SiO 2 Nanoparticular Coating to Prevent Bacterial Contamination in Blood Products
-
Fonseca S, Cayer MP, Ahmmed KMT, et al. ,
-
Antibiotics ,
-
2022,
-
11(1):107
- Instrument: NucleoCounter® NC-250™
- Cell Type: Whole Blood
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Technological innovations and quality control processes within blood supply organizations have significantly improved blood safety for both donors and recipients. Nevertheless, the risk of transfusion-transmitted infection remains non-negligible. Applying a nanoparticular, antibacterial coating at the surface of medical devices is...
View PublicationSoft-sensors application for automated feeding control in high-throughput mammalian cell cultures
-
Martínez-Monge I, Martínez C, Decker M, et al. ,
-
Biotechnol Bioeng ,
-
2022,
- Instrument: NucleoCounter® NC-250™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The ever-increasing demand for biopharmaceuticals has created the need for improving the overall productivity of culture processes. One such operational concept that is considered is fed-batch operations as opposed to batch operations. However, optimal fed-batch operations require complete knowledge of...
View PublicationTherapeutic Potential of Pretreatment with Exosomes Derived from Stem Cells from the Apical Papilla against Cisplatin-Induced Acute Kidney Injury
-
Huang TY, Chien MS, Su WT ,
-
Int J Mol Sci ,
-
2022,
-
23(10):5721
- Instrument: NucleoCounter® NC-250™
- Cell Type: Epithelial Cells
- Assay: Cell Vitality Assay
- Research area: Stem Cell Research
Acute kidney injury (AKI) is the most serious side effect of treatment with cisplatin in clinical practice. The aim of this study was to investigate the therapeutic effect of exosomes derived from stem cells from the apical papilla (SCAPs) on...
View PublicationPolyhexamethylene guanidine phosphate-induced necrosis may be linked to pulmonary fibrosis
-
Kang MS, Kim SH, Yang MJ, et al. ,
-
Toxicol Lett ,
-
2022,
-
362:1-16
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Following the humidifier disinfectant incident in Korea, polyhexamethylene guanidine phosphate (PHMG-P) has been used to establish lung fibrosis model animals. Herein, we investigated time-dependent changes after a single PHMG-P instillation (22 μg/lung) to identify the underlying pathogenesis and immune response...
View PublicationComprehensive Targeted Metabolomic Study in the Lung, Plasma, and Urine of PPE/LPS-Induced COPD Mice Model
-
Kim HY, Lee HS, Kim IH, et al. ,
-
Int J Mol Sci ,
-
2022,
-
23(5):2748
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
(1) Background: Progression of chronic obstructive pulmonary disease (COPD) leads to irreversible lung damage and inflammatory responses; however, biomarker discovery for monitoring of COPD progression remains challenging. (2) Methods: This study evaluated the metabolic mechanisms and potential biomarkers of COPD...
View PublicationSublethal pulmonary toxicity screening of silica nanoparticles in rats after direct intratracheal instillation
-
Han HY ,
-
Toxicological Research ,
-
2022,
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The present aimed to characterize the toxicity of silica nanoparticles in Sprague Dawley rats and determine the dose levels for a repeated-dose toxicity study. Silica nanoparticles (SiO2, 20 nm and 50 nm) were administered as a single intratracheal instillation of...
View PublicationSARS-CoV2 wild type and mutant specific humoral and T cell immunity is superior after vaccination than after natural infection
-
Richardson JR, Götz R, Mayr V, et al. ,
-
PLoS One ,
-
2022,
-
17(4):e0266701
- Instrument: NucleoCounter® NC-250™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Objective: We investigated blood samples from fully SARS-CoV2-vaccinated subjects and from previously positive tested patients up to one year after infection with SARS-CoV2, and compared short- and long-term T cell and antibody responses, with a special focus on the recently...
View PublicationDisruption of Membrane Integrity as a Molecular Initiating Event Determines the Toxicity of Polyhexamethylene Guanidine Phosphate Depending on the Routes of Exposure
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Song J, Jung KJ, Yang MJ, et al. ,
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Int J Mol Sci ,
-
2022,
-
23(6):3289
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Polyhexamethylene guanidine phosphate (PHMG-P), a cationic biocide, is widely used in household products due to its strong bactericidal activity and low toxicity. However, it causes fatal lung damage when inhaled. In this study, we investigated why PHMG-P causes fatal lung...
View PublicationThe effects of air pollutants exposure on the transmission and severity of invasive infection caused by an opportunistic pathogen Streptococcus pyogenes
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Zhi Y, Chen X, Cao G, et al. ,
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Environ Pollut ,
-
2022,
-
119826
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Currently, urbanization is associated with an increase in air pollutants that contribute to invasive pathogen infections by altering the host’s innate immunity and antimicrobial resistance capability. Streptococcus pyogenes, also known as Group A Streptococcus (GAS), is a gram-positive opportunistic pathogen...
View PublicationDNA fragmentation and membrane integrity in sperm of farmed Arctic charr (Salvelinus alpinus)
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Jeuthe H, Palaiokostas C, Johannisson A ,
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Aquaculture, ,
-
2022,
-
547:737537
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
This experiment was conducted to assess the effect of different antibiotics in tris-fructose egg yolk-based diluent on bacterial load and sperm quality of dromedary camels during processing and cold storage. Ten semen ejaculates were collected from five male dromedary camels....
View PublicationToward commercialization: Improvement of a semen cryopreservation protocol for European perch enables its implementation for commercial-scale fertilization
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Judycka S, Żarski D, Dietrich MA, et al. ,
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Aquaculture ,
-
2022,
-
549(737790)
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
This study emphasis improved semen cryopreservation protocol for commercial scale fertilization in European perch. The optimized procedure involves a new extender (GM 25 K) containing 0.30 M glucose, 7.5% methanol and 25 mM KCl at 3.0 × 109/mL spermatozoa. The...
View PublicationImpact of glyphosate and its formulation Roundup® on stallion spermatozoa
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Spinaci M, Nerozzi C, Mislei B, et al. ,
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Theriogenology ,
-
2022,
-
179:197-203
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
The growing and widespread use of glyphosate-based herbicides (GBHs) has raised an intense public debate about the impact of environmental contamination on animal and human health, including male fertility. The aim of this study was to deepen the impact of...
View PublicationPractical Method for Freezing Buck Semen
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Morrell JM, Malaluang P, Ntallaris T, Johannisson A ,
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Animals ,
-
2022,
-
12(3):352
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Although several protocols for cryopreserving buck semen are described in the literature, they differ widely in factors such as season and method of semen collection, extender and sperm concentration. Therefore, choosing a protocol that is suitable for a particular on-farm...
View PublicationEvaluating the potential of improving sperm quality traits in farmed Arctic charr (Salvelinus alpinus) using selective breeding
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Kurta K, Jeuthe H, de Koning DJ, Palaiokostas C ,
-
Aquaculture Reports ,
-
2022,
-
25(101234)
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Toward commercialization: Improvement of a semen cryopreservation protocol for European perch enables its implementation for commercial-scale fertilization
View PublicationNo increase in pregnancy rate of mares after preovulatory deep uterine horn application of misoprostol
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Donatsch L, Friker B, Sieme H, et al. ,
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Theriogenology ,
-
2022,
-
184:132-139
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
A potential source of fertility loss in mares is oviductal dysfunction, potentially caused by masses or debris in the lumen, that may prevent either sperm from reaching the fertilization site or the embryo from reaching the uterus. Recently a novel...
View PublicationSperm Gone Smart: A Portable Device (iSperm®) to Assess Semen Concentration and Motility in Dogs
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Domain G, Banchi P, Ali Hassan H, et al. ,
-
Animals (Basel) ,
-
2022,
-
12(5):652
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
The iSperm® is a portable device for semen analysis. This study aimed to investigate its correlation with a conventional computer-assisted sperm analyzer (ISAS®v1) for the assessment of semen concentration and kinematic parameters in dogs (n = 224). The intra-assay variability...
View PublicationThe Proteomic Modification of Buck Ejaculated Sperm Induced by the Cryopreservation Process
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Lv C, Liang J, Yang H, et al. ,
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Biopreserv Biobank ,
-
2022,
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Using two-dimensional electrophoresis along with mass spectroscopy, we have investigated how the cryopreservation process affected the protein profile of goat ejaculated sperm. In this study, five bucks were used for semen collection. After removal of seminal plasma, the Tris-based extender...
View PublicationInfluence of Single Layer Centrifugation with Canicoll on Semen Freezability in Dogs
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Domain G, Ali Hassan H, Wydooghe E, et al. ,
-
Animals (Basel) ,
-
2022,
-
12(6):714
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
This study evaluated how semen selection by single layer centrifugation (SLC) with Canicoll affects semen freezability in dogs. A total of eighteen ejaculates, collected from dogs with optimal and suboptimal semen quality (optimal: normal morphology (NM) ≥ 80%, n =...
View PublicationUse of specific mitochondrial complex inhibitors to investigate mitochondrial involvement on horse sperm motility and ROS production
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Giaretta E, Mislei B, Martínez-Pastor F, et al. ,
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Res Vet Sci ,
-
2022,
-
147:12-19
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Equine spermatozoa highly rely on oxidative phosphorylation for their energy management. The present work aimed to characterize the role of mitochondria on horse sperm motility and ROS production by incubating spermatozoa with specific inhibitors of the different mitochondrial complexes. Equine...
View PublicationLive cells are not affected by dead sperm in liquid boar semen: New insights based on a thermo-resistance test
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Quirino M, Jakop U, Mellagi APG, et al. ,
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Reprod Dom Anim ,
-
2022,
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
This study evaluated the effect of different proportions of dead spermatozoa on the quality of liquid boar semen during a thermo-resistance test (TRT). After 3 days of storage (17°C), 54 conventional artificial insemination semen doses (~23 × 106 sperm/ml in ~88 ml of BTS)...
View PublicationComparison of NUCLEOCOUNTER, ANDROVISION with Leja chambers and the newly developed ANDROVISION eFlow for sperm concentration analysis in boars
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Grossfeld R, Pable J, Jakop U, et al. ,
-
Sci Rep ,
-
2022,
-
12(1):11943
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Exact analysis of sperm concentration in raw and diluted semen is of major importance in swine artificial insemination, as sperm concentration is one of the most important characteristics of an ejaculate determining the value of the ejaculate and the productive...
View PublicationThe establishment of goat semen protein profile using a tandem mass tag-based proteomics approach
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Liang J, Lv C, Xiang D, et al. ,
-
Res Vet Sci ,
-
2022,
-
150:22-32
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
In this study, the complete proteome of goat ejaculated semen including spermatozoa and seminal plasma was established, applying a tandem mass tag (TMT) labeling together with liquid chromatography-tandem mass spectrometry (LC-MS/MS). In seminal plasma, 2299 proteins were identified and 2098...
View PublicationThe telomeric 5′ end nucleotide is regulated in the budding yeast Naumovozyma castellii
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Itriago H, Jaiswal RK, Philipp S, Cohn M ,
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Nucleic Acids Res ,
-
2022,
-
50(1):281-292
- Instrument: NucleoCounter® YC-100™
- Cell Type: Yeast Cells
- Research area: Brewing
The junction between the double-stranded and single-stranded telomeric DNA (ds-ss junction) is fundamental in the maintenance of the telomeric chromatin, as it directs the assembly of the telomere binding proteins. In budding yeast, multiple Rap1 proteins bind the telomeric dsDNA,...
View PublicationUtility of Islet Cell Preparations From Donor Pancreases After Euthanasia
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De Paep DL, Van Hulle F, Ling Z, et al. ,
-
Cell Transplant ,
-
2022,
-
31:9636897221096160
- Instrument: NucleoCounter® YC-100™
- Cell Type: Mammalian Cells
- Research area: Cell Biology
Patients fulfilling criteria for euthanasia can choose to donate their organs after circulatory death [donors after euthanasia (DCD V)]. This study assesses the outcome of islet cell isolation from DCD V pancreases. A procedure for DCD V procurement provided 13...
View PublicationScaffold-mediated switching of lymphoma metabolism in culture
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Bhatt R, Ravi D, Evens AM, Parekkadan B ,
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Cancer Metab ,
-
2022,
-
10(1):15
- Instrument: NucleoCounter® NC-202™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Background: Diffuse large B cell lymphoma (DLBCL) is an aggressive subtype of non-Hodgkin lymphoma (NHL) and accounts for about a third of all NHL cases. A significant proportion (~40%) of treated DLBCL patients develop refractory or relapsed disease due to...
View PublicationCircular single-stranded DNA is a superior homology-directed repair donor template for efficient genome engineering
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Xie K, Starzyk J, Majumdar I, et al. ,
-
bioRxiv ,
-
2022,
- Instrument: NucleoCounter® NC-202™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Gene Therapy
The toolbox for genome editing in basic research and therapeutic applications is rapidly expanding. While efficient targeted gene ablation using nuclease editors has been demonstrated from bench to bedside, precise transgene integration remains a technical challenge. AAV6 has been a...
View Publication2021
Assessment of Protocol Impact on Subjectivity Uncertainty When Analyzing Peripheral Blood Mononuclear Cell Flow Cytometry Data Files
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Grant R, Coopman K, Silva-Gomes S, et al. ,
-
Methods and Protocols ,
-
2021,
-
4(2):24
- Instrument: NucleoCounter® NC-3000™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Measured variability of product within Cell and Gene Therapy (CGT) manufacturing arises from numerous sources across pre-analytical to post-analytical phases of testing. Operators are a function of the manufacturing process and are an important source of variability as a result...
View PublicationTranscriptomic analysis suggested the involvement of impaired lipid droplet biogenesis in graphene oxide-induced cytotoxicity in human umbilical vein endothelial cells
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Luo Y, Wang X, Cao Y ,
-
Chemico-biological Interactions ,
-
2021,
-
333:109325
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Previous studies revealed that direct contact with graphene oxide (GO) induced cytotoxic effects, but the importance of involvement of metabolic pathways, in particular lipid metabolism pathways, might be overlooked. In this study, human umbilical vein endothelial cells (HUVECs) were exposed...
View PublicationNOX4 promotes ferroptosis of astrocytes by oxidative stress-induced lipid peroxidation via the impairment of mitochondrial metabolism in Alzheimer’s diseases
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Park MW, Cha HW, Kim J, et al. ,
-
Redox Biology ,
-
2021,
-
41:101947
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Mitochondrial Potential Assay
- Research area: Cell Biology
Oxidative stress has been implicated in the pathogenesis of Alzheimer’s disease (AD). Mitochondrial dysfunction is linked to oxidative stress and reactive oxygen species (ROS) in neurotoxicity during AD. Impaired mitochondrial metabolism has been associated with mitochondrial dysfunction in brain damage...
View PublicationThe impact of multimodal pore size considered independently from porosity on mechanical performance and osteogenic behaviour of titanium scaffolds
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Torres-Sanchez C, Norrito M, Almushref FR, Conway PP ,
-
Materials Science and Engineering C ,
-
2021,
-
124:112026
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Titanium porous scaffolds comprising multimodal pore ranges (i.e., uni-, bi-, tri-modal and random) were studied to evaluate the effect of pore size on osteoblastogenesis. The scaffolds were manufactured using spaceholder-powder metallurgy, and porosity and pore size were kept independent. Their...
View PublicationTart Cherry Extract Containing Chlorogenic Acid, Quercetin, and Kaempferol Inhibits the Mitochondrial Apoptotic Cell Death Elicited by Airborne PM10 in Human Epidermal Keratinocytes
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Kim DW, Jung DH, Sung J, et al. ,
-
Antioxidants ,
-
2021,
-
10(3):443
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Epithelial Cells
- Assay: Annexin V Assay
- Research area: Cell Biology
Tart cherry (Prunus cerasus L.), a medicinal food containing high concentrations of phytochemicals, has a variety of antioxidant activities and health benefits. Here, we investigate the functional effect of tart cherry during apoptotic cell death elicited by airborne particulate matter...
View PublicationSuperior Osteo-Inductive and Osteo-Conductive Properties of Trabecular Titanium vs. PEEK Scaffolds on Human Mesenchymal Stem Cells: A Proof of Concept for the Use of Fusion Cages
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Ragni E, Perucca Orfei C, Bidossi A, et al. ,
-
International Journal of Molecular Sciences ,
-
2021,
-
22(5):2379
- Instrument: NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Fusion cages composed of titanium and its alloys are emerging as valuable alternative to standard polyetheretherketone (PEEK) ones routinely used in cervical and lumbar spine surgery. Aim of this study was to evaluate osteo-inductive and osteo-conductive ability of an innovative...
View PublicationToxicity evaluation of mesoporous silica particles Santa Barbara No. 15 amorphous in human umbilical vein endothelial cells: influence of particle morphology
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Teng W, Yang Z, Wang S, et al. ,
-
Journal of Applied Toxicology ,
-
2021,
- Instrument: NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Morphology plays a vital role in determining the biological effects of silica nanoparticles (NPs), but its influence on the toxicity of silica NPs in endothelial cells (ECs) is still inconclusive. We synthesized five kinds of Santa Barbara 15 amorphous (SBA-15)...
View PublicationExtended lifespan of bronchial epithelial cells maintains normal cellular phenotype and transcriptome integrity
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O'Loughlin J, Hall RJ, Bhaker S, et al. ,
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ERJ Open Research ,
-
2021,
-
7(1):00254-2020
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay
- Research area: Gene Therapy
Genetic studies have identified several epithelial-derived genes associated with airway diseases. However, techniques used to study gene function frequently exceed the proliferative potential of primary human bronchial epithelial cells (HBECs) isolated from patients. Increased expression of the polycomb group protein...
View PublicationLongitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19
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Szabo PA, Dogra P, Gray JI, et al. ,
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Immunity ,
-
2021,
-
S1074-7613(21)00117-5
- Instrument: NucleoCounter® NC-3000™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Immunology
Immune response dynamics in coronavirus disease 2019 (COVID-19) and their severe manifestations have largely been studied in circulation. Here, we examined the relationship between immune processes in the respiratory tract and circulation through longitudinal phenotypic, transcriptomic, and cytokine profiling of...
View PublicationUrban air PM modifies differently immune defense responses against bacterial and viral infections in vitro
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Shahbaz MA, Martikainen MV, Rönkkö TJ, et al. ,
-
Environmental Research ,
-
2021,
-
192:110244
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Immunology
Epidemiological evidence has shown the association between exposure to ambient fine particulate matter (PM) and increased susceptibility to bacterial and viral respiratory infections. However, to date, the underlying mechanisms of immunomodulatory effects of PM remain unclear. Our objective was to...
View PublicationAdipose-Derived Stromal/Stem Cell Culture: Effects of Different Concentrations of Human Platelet Lysate in Media
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Ballesteros OR, Brooks PT, Haastrup EK, et al. ,
-
Cells Tissues Organs ,
-
2021,
-
01/Sep
- Instrument: NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay, Cell Vitality Assay
- Research area: Immunology
Adipose-derived stromal/stem cells (ASCs) are being tested as a possible treatment for a wide range of diseases to exploit the immunomodulatory and regenerative potential demonstrated in vitro. Pooled human platelet lysate (pHPL) has replaced fetal bovine serum (FBS) as the...
View PublicationBMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia
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Seipel K, Kopp B, Bacher U, Pabst T ,
-
Cancers ,
-
2021,
-
13(3):581
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay
- Research area: Oncology
Purpose: Prognosis for acute myeloid leukemia (AML) patients is poor, particularly in TP53 mutated AML, secondary, relapsed, and refractory AML, and in patients unfit for intensive treatment, thus highlighting an unmet need for novel therapeutic approaches. The combined use of...
View PublicationAllosteric and ATP-Competitive MEK-Inhibition in a Novel Spitzoid Melanoma Model with a RAF- and Phosphorylation-Independent Mutation
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Hegedüs L, Okumus Ö, Livingstone E, et al. ,
-
Cancers ,
-
2021,
-
13(4):829
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Spitzoid melanoma is a rare malignancy with histological characteristics similar to Spitz nevus. It has a diverse genetic background and in adults, a similarly grim clinical outcome as conventional malignant melanoma. We established a spitzoid melanoma cell line (PF130) from...
View PublicationThe role of UVA radiation in ketoprofen-mediated BRAF-mutant amelanotic melanoma cells death – A study at the cellular and molecular level
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Banach K, Kowalska J, Rzepka Z, et al. ,
-
Toxicology in Vitro ,
-
2021,
-
72:105108
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Count & Viability Assay, Cell Cycle Assay, Cell Vitality Assay, DNA Fragmentation Assay
- Research area: Oncology
Malignant melanoma is the cause of 80% of deaths in skin cancer patients. Treatment of melanoma in the 4th stage of clinical advancement, in which inoperable metastasis occur, does not provide sufficient effects. Ketoprofen has phototoxic properties and it can...
View PublicationResponse of Human Glioblastoma Cells to Vitamin B12 Deficiency: A Study Using the Non-Toxic Cobalamin Antagonist
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Rzepka Z, Rok J, Maszczyk M, et al. ,
-
Biology ,
-
2021,
-
10(1):69
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay
- Research area: Oncology
The most important biological function of vitamin B12 is to accomplish DNA synthesis, which is necessary for cell division. Cobalamin deficiency may be especially acute for rapidly dividing cells, such as glioblastoma cells. Therefore, cobalamin antagonists offer a medicinal potential...
View PublicationInfluence of Complexation of Thiosemicarbazone Derivatives with Cu (II) Ions on Their Antitumor Activity against Melanoma Cells
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Pitucha M, Korga-Plewko A, Czylkowska A, et al. ,
-
International Journal of Molecular Sciences ,
-
2021,
-
22(6):3104
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Annexin V Assay, Cell Cycle Assay
- Research area: Oncology
A series of thiosemicarbazone derivatives was prepared and their anti-tumor activity in vitro was tested. The X-ray investigation performed for compounds T2, T3 and T5 confirmed the synthesis pathway and assumed molecular structures of analyzed thiosemicarbazones. The conformational preferences of...
View PublicationLysosome-Instructed Self-Assembly of Amino-Acid-Functionalized Perylene Diimide for Multidrug-Resistant Cancer Cells
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Keum C, Hong J, Kim D, et al. ,
-
ACS Applied Materials & Interfaces ,
-
2021,
-
13(13):14866-14874
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Annexin V Assay
- Research area: Oncology
Multidrug resistance (MDR) of cancer cells reduces chemotherapeutic efficacy by preventing drug accumulation in the cells through a drug efflux pump and lysosomal sequestration/exocytosis. Herein, to overcome such anticancer resistance, lysosome-targeted self-assembly of perylene diimide (PDI) derivatives is presented as...
View PublicationMethyl gallate, gallic acid-derived compound, inhibit cell proliferation through increasing ROS production and apoptosis in hepatocellular carcinoma cells
-
Huang CY, Chang YJ, Wei PL, et al. ,
-
PLOS One ,
-
2021,
-
16(3):e0248521
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay, FlexiCyte™ Assay
- Research area: Oncology
Hepatocellular carcinoma (HCC) is a global health problem. Currently, there is no effective therapeutic strategy for HCC. Methyl gallate (MG), from plant-derived phenolic gallic acid, has exhibited antitumor efficacy. However, the effect of MG on HCC is unclear. In vitro...
View PublicationChimeric VLPs Based on HIV-1 Gag and a Fusion Rabies Glycoprotein Induce Specific Antibodies against Rabies and Foot-and-Mouth Disease Virus
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Fontana D, Garay E, Cervera L, et al. ,
-
Vaccines ,
-
2021,
-
9(3):251
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Protein Production
Foot and mouth disease is a livestock acute disease, causing economic losses in affected areas. Currently, control of this disease is performed by mandatory vaccination campaigns using inactivated viral vaccines. In this work, we describe the development of a chimeric...
View PublicationMinocycline Impact on Redox Homeostasis of Normal Human Melanocytes HEMn-LP Exposed to UVA Radiation and Hydrogen Peroxide
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Rok J, Rzepka Z, Maszczyk M, et al. ,
-
International Journal of Molecular Sciences ,
-
2021,
-
22(4):1642
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Small Molecule Drugs
Minocycline is a semisynthetic tetracycline antibiotic. In addition to its antibacterial activity, minocycline shows many non-antibiotic, beneficial effects, including antioxidative action. The property is responsible, e.g., for anti-inflammatory, neuroprotective, and cardioprotective effects of the drug. However, long-term pharmacotherapy with minocycline...
View PublicationA novel non-viral delivery method that enables efficient engineering of primary human T cells for ex vivo cell therapy applications
-
Kavanagh H, Dunne S, Martin DS, et al. ,
-
Cytotherapy ,
-
2021,
-
23(9):852-860
- Instrument: NucleoCounter® NC-3000™
- Cell Type: T Cells
- Assay: Cell Count & Viability Assay, GFP Transfection Efficiency Assay
- Research area: Cell-based Immunotherapy
Background aims: Next-generation immune cell therapy products will require complex modifications using engineering technologies that can maintain high levels of cell functionality. Non-viral engineering methods have the potential to address limitations associated with viral vectors. However, while electroporation is the...
View PublicationAltered network and rescue of human neurons derived from individuals with early-onset genetic epilepsy
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Negraes PD, Trujillo CA, Yu NK, et al. ,
-
Mol Psychiatry ,
-
2021,
-
2021 Apr 22
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Neural Cells
- Assay: DNA Fragmentation Assay, Mitochondrial Potential Assay
- Research area: Neurobiology
Early-onset epileptic encephalopathies are severe disorders often associated with specific genetic mutations. In this context, the CDKL5 deficiency disorder (CDD) is a neurodevelopmental condition characterized by early-onset seizures, intellectual delay, and motor dysfunction. Although crucial for proper brain development, the...
View PublicationThe antidepressant Sertraline inhibits CatSper Ca2+ channels in human sperm
-
Rahban R, Rehfeld A, Schiffer C, et al. ,
-
Hum Reprod ,
-
2021,
-
36(10):2638-2648
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Sperm Cells
- Assay: Cell Count & Viability Assay
- Research area: Animal Reproduction
STUDY QUESTION Do selective serotonin reuptake inhibitor (SSRI) antidepressants affect the function of human sperm? SUMMARY ANSWER The SSRI antidepressant Sertraline (e.g. Zoloft) inhibits the sperm-specific Ca2+ channel CatSper and affects human sperm function in vitro. WHAT IS KNOWN ALREADY...
View PublicationAreca nut extract (ANE) inhibits the progression of hepatocellular carcinoma cells via activation of ROS production and activation of autophagy
-
Wei PL, Hung CS, Lu HH, et al. ,
-
Int J Med Sci ,
-
2021,
-
18(15):3452-3462
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Hepatocellular carcinoma (HCC) is a worldwide health problem. Currently, there is no effective therapeutic strategy for HCC patients. Chewing areca nut is closely associated with oral cancer and liver cirrhosis. The therapeutic effect of areca nut extract (ANE) on HCC...
View PublicationThe Anti-Obesity Effects of Lemon Fermented Products in 3T3-L1 Preadipocytes and in a Rat Model with High-Calorie Diet-Induced Obesity
-
Wu CC, Huang YW, Hou CY, et al. ,
-
Nutrients ,
-
2021,
-
13(8):2809
- Instrument: NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Lemon (Citrus limon) has antioxidant, immunoregulatory, and blood lipid-lowering properties. This study aimed to determine the effect of the lemon fermented product (LFP) which is lemon fermented with Lactobacillus OPC1 to prevent obesity. The inhibition of lipid accumulation in 3T3-L1...
View PublicationIn Vitro Toxicological Assessment of Gadodiamide in Normal Brain SVG P12 Cells
-
Tsai YF, Yang JS, Tsai FJ, et al. ,
-
In Vivo ,
-
2021,
-
35(5):2621-2630
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Neural Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background/aim: Magnetic resonance imaging (MRI) is a technique for evaluating patients with primary and metastatic tumors. The contrast agents improve the diagnostic accuracy of MRI. Large quantities of a contrast agent must be administrated into the patient to obtain useful...
View PublicationComparison of miRNA cargo in human adipose-tissue vs. amniotic-membrane derived mesenchymal stromal cells extracellular vesicles for osteoarthritis treatment
-
Ragni E, Perucca Orfei C, Papait A, de Girolamo L ,
-
Extracell Vesicles Circ Nucleic Acids ,
-
2021,
-
2:202-21
- Instrument: NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Aim: Mesenchymal stromal cells (MSCs) emerged as a promising therapeutic option for osteoarthritis (OA) management, in particular those isolated from adipose tissue (hASCs) and amniotic membrane (hAMSCs). The cartilage protective and immunomodulatory features of hASCs and hAMSCs are ascribed to...
View PublicationCharacterization of a functional endothelial super-enhancer that regulates ADAMTS18 and angiogenesis
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Mushimiyimana I, Niskanen H, Beter M, et al. ,
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Nucleic Acids Res ,
-
2021,
-
49(14):8078-8096
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Epithelial Cells
- Assay: Cell Cycle Assay
- Research area: Cell Biology
Super-enhancers are clusters of enhancers associated with cell lineage. They can be powerful gene-regulators and may be useful in cell-type specific viral-vector development. Here, we have screened for endothelial super-enhancers and identified an enhancer from within a cluster that conferred...
View PublicationIn vitro-in vivo correlations of pulmonary inflammogenicity and genotoxicity of MWCNT
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Di Ianni E, Erdem JS, Møller P, et al. ,
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Part Fibre Toxicol ,
-
2021,
-
18(1):25
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Multi-walled carbon nanotubes (MWCNT) have received attention due to extraordinary properties, resulting in concerns for occupational health and safety. Costs and ethical concerns of animal testing drive a need for in vitro models with predictive power in respiratory toxicity....
View PublicationIn Vitro Study of Human Immune Responses to Hyaluronic Acid Hydrogels, Recombinant Spidroins and Human Neural Progenitor Cells of Relevance to Spinal Cord Injury Repair
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Lin C, Ekblad-Nordberg Å, Michaëlsson J, et al. ,
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Cells ,
-
2021,
-
10(7):1713
- Instrument: NucleoCounter® NC-3000™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Scaffolds of recombinant spider silk protein (spidroin) and hyaluronic acid (HA) hydrogel hold promise in combination with cell therapy for spinal cord injury. However, little is known concerning the human immune response to these biomaterials and grafted human neural stem/progenitor...
View PublicationDifferentiation of human induced pluripotent stem cells to authentic macrophages using a defined, serum-free, open-source medium
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Vaughan-Jackson A, Stodolak S, Ebrahimi KH, et al. ,
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Stem Cell Reports ,
-
2021,
-
16(7):1735-1748
- Instrument: NucleoCounter® NC-3000™
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Human induced pluripotent stem cells (iPSCs) and macrophages derived from them are increasingly popular tools for research into both infectious and degenerative diseases. However, as the field strives for greater modeling accuracy, it is becoming ever more challenging to justify...
View PublicationPsoriasis-like Inflammation Induced in an Air-pouch Mouse Model
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Charitidis FT, Damlund DSM, Koch J ,
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In Vivo ,
-
2021,
-
35(4):1985-1997
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Background/aim: The pathway of initiation of psoriasis comprises the differentiation and infiltration of T-helper 17 (Th17) cells into the skin, characterized by the production of interleukin 17A and 17F (IL-17A/IL-17F) among other cytokines, resulting in a downstream cascade of events....
View PublicationComparison of multi-walled carbon nanotubes and halloysite nanotubes on lipid profiles in human umbilical vein endothelial cells
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Liu Y, Hu Q, Huang C, Cao Y ,
-
Nanoimpact ,
-
2021,
-
23:100333
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Tubular nanomaterials (NMs), such as multi-walled carbon nanotubes (MWCNTs) and halloysite nanotubes (HNTs), may be used in biomedicine, but previous studies showed that MWCNTs induced toxicity to endothelial cells (ECs). However, the influence of tubular NMs on EC lipid profiles...
View PublicationUPF1 promotes the formation of R loops to stimulate DNA double-strand break repair
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Ngo GHP, Grimstead JW, Baird DM ,
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Nat Commun ,
-
2021,
-
12(1):3849
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay, Cell Cycle Assay, GFP Transfection Efficiency Assay
- Research area: Cell Biology
DNA-RNA hybrid structures have been detected at the vicinity of DNA double-strand breaks (DSBs) occurring within transcriptional active regions of the genome. The induction of DNA-RNA hybrids strongly affects the repair of these DSBs, but the nature of these structures...
View PublicationA comparative study of toxicity of graphdiyne and graphene oxide to human umbilical vein endothelial cells
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Cao Y, Xiao W, Li S, Qiu D ,
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J Appl Toxicol ,
-
2021,
-
41(12):2021-2030
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Primary Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The success of graphene oxide (GO) has attracted extensive research interests in developing novel 2D nanomaterials (NMs). Graphdiyne (GDY) is a new member of carbon-based 2D NMs possessing sp- and sp2 -hybridized carbon atoms. However, the toxicity of GDY is...
View PublicationArginine is an epigenetic regulator targeting TEAD4 to modulate OXPHOS in prostate cancer cells
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Chen CL, Hsu SC, Chung TY, et al. ,
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Nat Commun ,
-
2021,
-
12(1):2398
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Arginine plays diverse roles in cellular physiology. As a semi-essential amino acid, arginine deprivation has been used to target cancers with arginine synthesis deficiency. Arginine-deprived cancer cells exhibit mitochondrial dysfunction, transcriptional reprogramming and eventual cell death. In this study, we...
View PublicationDMSO (Me2SO) concentrations of 1-2% in combination with pentaisomaltose are effective for cryopreservation of T cells
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Haastrup EK, Munthe-Fog L, Ballesteros OR, et al. ,
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Transfus Apher Sci ,
-
2021,
-
60(4):103138
- Instrument: NucleoCounter® NC-3000™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
T cell based treatments in the setting of allogenic haematopoietic stem cell transplantation (HSCT) have been used for decades. In addition, the use of chimeric antigen receptor (CAR) T cells has been introduced as a promising cancer immunotherapy. A prerequisite...
View PublicationDesign, Synthesis, Characterization, and Crystal Structure Studies of Nrf2 Modulators for Inhibiting Cancer Cell Growth In Vitro and In Vivo
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Chikkegowda P, Pookunoth BC, Bovilla VR, et al. ,
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ACS Omega ,
-
2021,
-
6(15):10054-10071
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Nrf2 is one of the important therapeutic targets studied extensively in several cancers including the carcinomas of the colon and rectum. However, to date, not many Nrf2 inhibitors showed promising results for retarding the growth of colorectal cancers (CRCs). Therefore,...
View PublicationAberrantly reduced expression of miR-342-5p contributes to CCND1-associated chronic myeloid leukemia progression and imatinib resistance
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Wu YY, Lai HF, Huang TC, et al. ,
-
Cell Death Dis ,
-
2021,
-
12(10):908
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Oncology
Chronic myeloid leukemia (CML) is a myeloproliferative disorder associated with the Philadelphia chromosome, and the current standard of care is the use of tyrosine kinase inhibitors (TKI). However, some patients will not achieve a molecular response and may progress to...
View PublicationTiO2 nanosheets promote the transformation of vascular smooth muscle cells into foam cells in vitro and in vivo through the up-regulation of nuclear factor kappa B subunit 2
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Li S, Yan D, Huang C, et al. ,
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J Hazard Mater ,
-
2021,
-
127704
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Titanium dioxide (TiO2) nanomaterials have been shown to promote atherosclerosis through endothelial dysfunction. This study investigated the toxicity of TiO2 nanosheets (NSs) to vascular smooth muscle cells (VSMCs), one of the pivotal cells involved in all stages of atherosclerosis. Only...
View PublicationMicrofluidic transfection of mRNA into human primary lymphocytes and hematopoietic stem and progenitor cells using ultra-fast physical deformations
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Loo J, Sicher I, Goff A, et al. ,
-
Sci Rep ,
-
2021,
-
11(1):21407
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay, GFP Transfection Efficiency Assay
- Research area: Cell-based Immunotherapy
Messenger RNA (mRNA) delivery provides gene therapy with the potential to achieve transient therapeutic efficacy without risk of insertional mutagenesis. Amongst other applications, mRNA can be employed as a platform to deliver gene editing molecules, to achieve protein expression as...
View PublicationTranscriptomic-based toxicological investigations of graphene oxide with modest cytotoxicity to human umbilical vein endothelial cells: changes of Toll-like receptor signaling pathways
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Luo Y, Wang X, Cao Y ,
-
Toxicology Research ,
-
2021,
-
tfab091
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The wide uses of graphene oxide (GO) lead to the contact of GO with vascular systems, so it is necessary to investigate the toxicological effects of GO to endothelial cells. Recently, we reported that GO of small lateral size (
View PublicationAutomation enables high-throughput and reproducible single-cell transcriptomics library preparation
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Kind D, Baskaran P, Ramirez F, et al. ,
-
SLAS Technol ,
-
2021,
-
S2472-6303(21)00018-2
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Next-generation sequencing (NGS) has revolutionized genomics, decreasing sequencing costs and allowing researchers to draw correlations between diseases and DNA or RNA changes. Technical advances have enabled the analysis of RNA expression changes between single cells within a heterogeneous population, known...
View PublicationThermal conditioning improves quality and speed of keratinocyte sheet production for burn wound treatment
-
Frese L, Darwiche SE, von Rechenberg B, et al. ,
-
Cytotherapy ,
-
2021,
-
S1465-3249(21)00031-1
- Instrument: NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background aims: Cultured patient-specific keratinocyte sheets have been used clinically since the 1970s for the treatment of large severe burns. However, despite significant developments in recent years, successful and sustainable treatment is still a challenge. Reliable, high-quality grafts with faster...
View PublicationActivin A‐derived human embryonic stem cells show increased competence to differentiate into primordial germ cell‐like cells
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Mishra S, Taelman J, Popovic M, et al. ,
-
Stem Cells ,
-
2021,
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Protocols for specifying human primordial germ cell-like cells (hPGCLCs) from human embryonic stem cells (hESCs) remain hindered by differences between hESC lines, their derivation methods, and maintenance culture conditions. This poses significant challenges for establishing reproducible in vitro models of...
View PublicationCombination effect of nanoparticles on the acute pulmonary inflammogenic potential: additive effect and antagonistic effect
-
Lee S, Lee DK, Jeon S, et al. ,
-
Nanotoxicology ,
-
2021,
-
15(2):276-288
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The combination effect of co-exposed different types of nanomaterials is little known although humans are generally exposed to a mixture of nanomaterials from urban ultrafine particles or industrial nanomaterials. Herein, we evaluated the combined effect of nanoparticles (NPs) using three...
View PublicationOvercoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactors
-
Borys BS, Dang T, So T, et al. ,
-
Stem Cell Research & Therapy ,
-
2021,
-
12(1):55
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Human induced pluripotent stem cells (hiPSCs) hold enormous promise in accelerating breakthroughs in understanding human development, drug screening, disease modeling, and cell and gene therapies. Their potential, however, has been bottlenecked in a mostly laboratory setting due to bioprocess...
View PublicationMicroRNAs and Regulation of Autophagy in Chondrocytes
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Ramos YFM, Mobasheri A ,
-
Methods in Molecular Biology ,
-
2021,
-
2245:179-194
- Instrument: NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Chondrocytes are the main cells responsible for the maintenance of cartilage homeostasis and integrity. During development, extracellular matrix (ECM) macromolecules are produced and deposited by chondrocyte precursors. Autophagy, a highly dynamic process aimed at degradation of dysfunctional or pathogenic proteins,...
View PublicationCombination therapy with Treg and mesenchymal stromal cells enhances potency and attenuation of inflammation after traumatic brain injury compared to monotherapy
-
Caplan HW, Prabhakara KS, Toledano Furman NE, et al. ,
-
Stem cells ,
-
2021,
-
39(3):358-370
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
The inflammatory response after traumatic brain injury (TBI) can lead to significant secondary brain injury and chronic inflammation within the central nervous system. Cell therapies, including mesenchymal stromal cells (MSC), have led to improvements in animal models of TBI and...
View PublicationSeverity of heterosubtypic influenza virus infection in ferrets is reduced by live attenuated influenza vaccine
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Marriott AC, Gooch KE, Brown PJ, et al. ,
-
NPJ Vaccines ,
-
2021,
-
6(1):43
- Instrument: NucleoCounter® NC-200™
- Cell Type: Whole Blood
- Assay: Cell Count & Viability Assay
- Research area: Immunology
Live attenuated influenza vaccine (LAIV) is widely used to protect humans from seasonal influenza infection, particularly in children. In contrast to inactivated vaccines, the LAIV can induce both mucosal and cellular immune responses. Here we show that a single dose...
View PublicationDicentracin-Like from Asian sea bass Fish and Moronecidine-Like from Hippocampus Comes: Two Candidate Antimicrobial Peptides Against Leishmanina major Infection
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Mohammadi M, Moradi Hasan-Abad A, Dehghani P, et al. ,
-
International Journal of Peptide Research and Therapeutics ,
-
2021,
-
27:769-778
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Immunology
Anti-Leishmanial drug therapy faces significant challenges related to cytotoxicity and drug resistance. Thus, new and efficient anti-Leishmanial drugs need to be identified. Due to their broad-spectrum antimicrobial and also immunomodulatory activities, antimicrobial peptides (AMPs) have attracted considerable attention. In this...
View PublicationCholesterol loading suppresses the atheroinflammatory gene polarization of human
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Lappalainen J, Yeung N, Nguyen SD, et al. ,
-
Scientific Reports ,
-
2021,
-
11(1):4923
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Immunology
In atherosclerotic lesions, blood-derived monocytes differentiate into distinct macrophage subpopulations, and further into cholesterol-filled foam cells under a complex milieu of cytokines, which also contains macrophage-colony stimulating factor (M-CSF) and granulocyte-macrophage-colony stimulating factor (GM-CSF). Here we generated human macrophages in...
View PublicationTherapeutic potential of Fingolimod in triple negative breast cancer preclinical models
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Rupp T, Pelouin O, Genest L, et al. ,
-
Translational Oncology ,
-
2021,
-
14(1):100926
- Instrument: NucleoCounter® NC-200™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Surgery followed by a chemotherapy agent is the first-line treatment for breast cancer patients. Nevertheless, new targets are required for women with triple-negative breast cancer (TNBC) in order to improve the treatment of this aggressive cancer subtype. Multiple pro-inflammatory molecules...
View PublicationAntihormone treatment differentially regulates PSA secretion, PSMA expression and 68Ga–PSMA uptake in LNCaP cells
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Mathy CS, Mayr T, Kürpig S, et al. ,
-
Journal of Cancer Research and Clinical Oncology ,
-
2021,
- Instrument: NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Background: In recent years, a variety of innovative therapeutics for castration-resistant prostate cancer have been developed, including novel anti-androgenic drugs, such as abiraterone or VPC-13566. Therapeutic monitoring of these pharmaceuticals is performed either by measuring PSA levels in serum or...
View PublicationChemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells
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Panella S, Muoio F, Jossen V, et al. ,
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Cells ,
-
2021,
-
10(2):466
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Adipose tissue is an abundant source of stem cells. However, liposuction cannot yield cell quantities sufficient for direct applications in regenerative medicine. Therefore, the development of GMP-compliant ex vivo expansion protocols is required to ensure the production of a “cell...
View PublicationHuman Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status
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Muoio F, Panella S, Jossen V, et al. ,
-
J Funct Biomater ,
-
2021,
-
12(2):25
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Human adipose stem cells (hASCs) are promising candidates for cell-based therapies, but they need to be efficiently expanded in vitro as they cannot be harvested in sufficient quantities. Recently, dynamic bioreactor systems operated with microcarriers achieved considerable high cell densities....
View PublicationMitochondrial augmentation of CD34 + cells from healthy donors and patients with mitochondrial DNA disorders confers functional benefit
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Jacoby E, Ben Yakir-Blumkin M, Blumenfeld-Kan S, et al. ,
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NPJ Regen Med ,
-
2021,
-
6(1):58
- Instrument: NucleoCounter® NC-200™
- Cell Type: HSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Mitochondria are cellular organelles critical for numerous cellular processes and harboring their own circular mitochondrial DNA (mtDNA). Most mtDNA associated disorders (either deletions, mutations, or depletion) lead to multisystemic disease, often severe at a young age, with no disease-modifying therapies....
View PublicationImpact of allogeneic feline uterine-derived mesenchymal stromal cell intravenous treatment on renal function of nephrectomized cats with chronic kidney disease
-
Zacharias S, Welty MB, Sand TT, Black LL ,
-
Research in Veterinary Science ,
-
2021,
-
2021 Sep 30
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
Chronic kidney disease (CKD) is a common condition and leading cause of mortality in cats. Mesenchymal stromal cells (MSCs) may have a therapeutic effect on CKD. The aim of this pilot study was to determine efficacy of systemically-administered allogeneic uterine...
View PublicationExtracellular vesicles from adipose stromal cells combined with a thermoresponsive hydrogel prevent esophageal stricture after extensive endoscopic submucosal dissection in a porcine model
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Coffin E, Grangier A, Perrod G, et al. ,
-
Nanoscale ,
-
2021,
-
13(35):14866-14878
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
In this study, we investigated the combination of extracellular (nano) vesicles (EVs) from pig adipose tissue-derived stromal cells (ADSCs) and a thermoresponsive gel, Pluronic® F-127 (PF-127), to prevent stricture formation after endoscopic resection in a porcine model. ADSC EVs were...
View PublicationDesensitization of human breast progenitors by a transient exposure to pregnancy levels of estrogen
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Rønnov-Jessen L, Kim J, Goldhammer N, et al. ,
-
Sci Rep ,
-
2021,
-
11(1):17232
- Instrument: NucleoCounter® NC-200™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Full term pregnancy at an early age is the only factor known to consistently protect against breast cancer. Because hormone receptor positive progenitors in the human breast relay endocrine signaling, we here sought to determine whether an experimental mimicry of...
View PublicationHuman Adipose Derived Stem Cells Enhance Healing in a Rat Model of Esophageal Injury with Stent
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McCloskey D, Linden K, Lin A, et al. ,
-
J Surg Res ,
-
2021,
-
267:458-466
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Mesenchymal stem cells have been proven to promote cellular recruitment and remodeling during healing. Considering challenges encountered in the healing process of esophageal injury, we sought to evaluate the effect of human adipose derived stem cells (hASC) on esophageal...
View PublicationFlow Cytometric Analysis Revealed a Significant Accumulation of Terminally Differentiated T cell Subtypes in the Circulating Lymphocytes of Cytomegalovirus (CMV) Positive Follicular Lymphoma Patients
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Lugos MD, Dangana A, Ntuhun BD, et al. ,
-
Journal of Clinical & Biomedical Research ,
-
2021,
-
01/Sep
- Instrument: NucleoCounter® NC-200™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Follicular lymphoma (FL), a non-Hodgkin lymphoma, is an indolent cancer of the B cell lineage that runs a chronic deterioration course that can result in multiple treatment episodes leading to resistance and possible transformation to diffuse large B cell lymphoma....
View PublicationThermal proteome profiling identifies PIP4K2A and ZADH2 as off-targets of Polo-like kinase 1 inhibitor volasertib
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Goroshchuk O, Kolosenko I, Kunold E, et al. ,
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FASEB J ,
-
2021,
-
35(7):e21741
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Polo-like kinase 1 (PLK1) is an important cell cycle kinase and an attractive target for anticancer treatments. An ATP-competitive small molecular PLK1 inhibitor, volasertib, has reached phase III in clinical trials in patients with refractory acute myeloid leukemia as a...
View PublicationSmall Intestinal Tuft Cell Activity Associates With Energy Metabolism in Diet-Induced Obesity
-
Arora P, Andersen D, Moll JM, et al. ,
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Front Immunol ,
-
2021,
-
12:629391
- Instrument: NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Little is known about the involvement of type 2 immune response-promoting intestinal tuft cells in metabolic regulation. We here examined the temporal changes in small intestinal tuft cell number and activity in response to high-fat diet-induced obesity in mice and...
View PublicationInflammatory Response, Reactive Oxygen Species Production and DNA Damage in Mice After Intrapleural Exposure to Carbon Nanotubes
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Wils RS, Jacobsen NR, Vogel U, et al. ,
-
Toxicol Sci ,
-
2021,
-
183(1):184-194
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Carbon nanotubes (CNTs) are speculated to cause mesothelioma by persistent inflammation, oxidative stress, tissue injury, and genotoxicity. To investigate the pleural response to CNTs, we exposed C57BL/6 mice by intrapleural injection of 0.2 or 5 µg multiwalled CNTs (MWCNT-7, NM-401,...
View PublicationHuman-derived Treg and MSC combination therapy may augment immunosuppressive potency in vitro, but did not improve blood brain barrier integrity in an experimental rat traumatic brain injury model
-
Caplan HW, Prabhakara KS, Toledano Furman NE, et al. ,
-
PLoS One ,
-
2021,
-
16(5):e0251601
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Traumatic brain injury (TBI) causes both physical disruption of the blood brain barrier (BBB) and altered immune responses that can lead to significant secondary brain injury and chronic inflammation within the central nervous system (CNS). Cell therapies, including mesenchymal stromal...
View PublicationMSC Manufacturing for Academic Clinical Trials: From a Clinical-Grade to a Full GMP-Compliant Process
-
Lechanteur C, Briquet A, Bettonville V, et al. ,
-
Cells ,
-
2021,
-
10(6):1320
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Following European regulation 1394/2007, mesenchymal stromal cell (MSCs) have become an advanced therapy medicinal product (ATMP) that must be produced following the good manufacturing practice (GMP) standards. We describe the upgrade of our existing clinical-grade MSC manufacturing process to obtain...
View PublicationSupporting dataset of two integration-free induced pluripotent stem cell lines from related human donors
-
Ramme AP, Faust D, Koenig L, et al. ,
-
Data Brief ,
-
2021,
-
37:107140
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Integration-free induced pluripotent stem cells from related human donors’ exhibit great potential to the ongoing development of organ models. Blood cells from two different human donors were isolated, purified and reprogrammed into induced pluripotent stem cells. These induced pluripotent stem...
View PublicationA Novel Method to Gently Mix and Uniformly Suspend Particulates for Automated Assays
-
Myers KK, Herich JP, Chavez JE, et al. ,
-
SLAS Technol ,
-
2021,
-
26(5):498-509
- Instrument: NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The SpinVessel system provides a methodology using pulsed radial flow to gently mix and uniformly suspend particulates (cells, magnetic beads, silica beads, and microcarrier beads) for automated assays. SpinVessels are well suited for aliquoting on robotic liquid handlers and with...
View PublicationHypoxia drives murine neutrophil protein scavenging to maintain central carbon metabolism
-
Watts ER, Howden AJ, Morrison T, et al. ,
-
J Clin Invest ,
-
2021,
-
131(10):e134073
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Limiting dysfunctional neutrophilic inflammation while preserving effective immunity requires a better understanding of the processes that dictate neutrophil function in the tissues. Quantitative mass-spectrometry identified how inflammatory murine neutrophils regulated expression of cell surface receptors, signal transduction networks, and metabolic...
View PublicationImpact of lipopolysaccharides on cultivation and recombinant protein expression in human embryonal kidney (HEK‐293) cells
-
Faust C, Beil C, Dittrich W, et al. ,
-
Eng Life Sci ,
-
2021,
-
21(11):778-785
- Instrument: NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The human embryonal kidney 293 cell (HEK-293) is a widely used expression host for transient gene expression. The genes or plasmids used for the transient transfections are usually propagated and extracted from the gram-negative bacterium Escherichia coli, the workhorse for...
View PublicationDevelopment of a human umbilical cord-derived mesenchymal stromal cell-based advanced therapy medicinal product to treat immune and/or inflammatory diseases
-
Mebarki M, Iglicki N, Marigny C, et al. ,
-
Stem Cell Research & Therapy ,
-
2021,
-
0,896527778
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Background Umbilical cord-derived mesenchymal stromal cells (UC-MSCs) revealed their key role in immune regulation, offering promising therapeutic perspectives for immune and inflammatory diseases. We aimed to develop a production process of an UC-MSC-based product and then to characterize UC-MSC properties...
View PublicationProlonged storage of purified granulocyte concentrates: Introduction of a new purification method
-
Klinkmann G, Doss F, Goudeva L, et al. ,
-
Transfusion ,
-
2021,
- Instrument: NucleoCounter® NC-200™
- Cell Type: PBMCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Background: Use of donor granulocyte concentrate (GC) has been limited due to its short storage time of 6-24 h, which is partially due to residual red blood cells (RBCs) and platelets and the resulting lactate production leading to an acidotic...
View PublicationProton-transfer-reaction mass spectrometry (PTR-MS) for online monitoring of glucose depletion and cell concentrations in HEK 293 gene therapy processes
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Bayer B, Maccani A, Jahn J, et al. ,
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Biotechnol Lett ,
-
2021,
- Instrument: NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Gene Therapy
Objectives: The applicability of proton-transfer-reaction mass spectrometry (PTR-MS) as a versatile online monitoring tool to increase consistency and robustness for recombinant adeno-associated virus (rAAV) producing HEK 293 bioprocesses was evaluated. We present a structured workflow to extract process relevant information...
View PublicationSingle-cell RNA-seq of out-of-thaw mesenchymal stromal cells shows tissue-of-origin differences and inter-donor cell-cycle variations
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Medrano-Trochez C, Chatterjee P, Pradhan P, et al. ,
-
Stem Cell Res Ther ,
-
2021,
-
12(1):565
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Preparation and growth factor characterization of cord blood-derived plasma, serum, growth factor-rich plasma and induced serum
View PublicationNanomaterial- and shape-dependency of TLR2 and TLR4 mediated signaling following pulmonary exposure to carbonaceous nanomaterials in mice
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Danielsen PH, Bendtsen KM, Knudsen KB, et al. ,
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Part Fibre Toxicol ,
-
2021,
-
18(1):40
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Background: Human Mesenchymal stromal cells (hMSCs) from various tissue sources are widely investigated in clinical trials. These MSCs are often administered to patients immediately after thawing the cryopreserved product (out-of-thaw), yet little is known about the single-cell transcriptomic landscape and...
View PublicationA double-blinded placebo-controlled evaluation of adipose-derived mesenchymal stem cells in treatment of canine atopic dermatitis
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Kaur G, Ramirez A, Xie C, et al. ,
-
Vet Res Commun ,
-
2021,
- Instrument: NucleoCounter® NC-200™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Background: Pulmonary exposure to high doses of engineered carbonaceous nanomaterials (NMs) is known to trigger inflammation in the lungs paralleled by an acute phase response. Toll-like receptors (TLRs), particularly TLR2 and TLR4, have recently been discussed as potential NM-sensors, initiating...
View PublicationEarly-life inflammation primes a T helper 2 cell-fibroblast niche in skin
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Boothby IC, Kinet MJ, Boda DP, et al. ,
-
Nature ,
-
2021,
-
599(7886):667-672
- Instrument: NucleoCounter® NC-200™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Mesenchymal stem cells (MSCs) have emerged as a new therapy for various immune-mediated inflammatory diseases. In this study we perform the first double-blinded, placebo-controlled evaluation of the efficacy of adipose-derived allogenic canine MSCs for the treatment of canine atopic dermatitis...
View PublicationOLFM4-RET fusion is an oncogenic driver in small intestine adenocarcinoma
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Liu W, Li H, Aerbajinai W, et al. ,
-
Oncogene ,
-
2021,
- Instrument: NucleoCounter® NC-200™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Research
Inflammation early in life can prime the local immune milieu of peripheral tissues, which can cause lasting changes in immunological tone that confer disease protection or susceptibility1. The cellular and molecular mechanisms that prompt changes in immune tone in many...
View PublicationAutologous Induced Pluripotent Stem Cell-Based Cell Therapies: Promise, Progress, and Challenges
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Madrid M, Sumen C, Aivio S, Saklayen N ,
-
Curr Protoc ,
-
2021,
-
1(3):e88
- Instrument: NucleoCounter® NC-200™
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
Small intestine adenocarcinoma is a rare intestinal malignancy with distinct clinical, pathological, and molecular characteristics. Recently, a fusion of the intestinal stem-cell marker olfactomedin 4 (OLFM4) and the proto-oncogene RET has been identified in a small intestine adenocarcinoma patient. Here...
View PublicationAccelerating manufacturing to enable large-scale supply of a new adenovirus-vectored vaccine within 100 days
-
Joe CCD, Segireddy RR, Oliveira C, et al. ,
-
bioRxiv ,
-
2021,
-
12.22.473478
- Instrument: NucleoCounter® NC-202™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Virus Production
The Coalition for Epidemic Preparedness Innovations’ ‘100-day moonshot’ aspires to launch a new vaccine within 100 days of pathogen identification. Here, we describe work to optimize adenovirus vector manufacturing for rapid response, by minimizing time to clinical trial and first...
View PublicationChitosan Membranes Filled with Cyclosporine A as Possible Devices for Local Administration of Drugs in the Treatment of Breast Cancer
-
Trombino S, Curcio F, Poerio T, et al. ,
-
Molecules ,
-
2021,
-
26(7):1889
- Instrument: NucleoCounter® NC-202™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
The aim of this work is the design, preparation and characterization of membranes based on cyclosporine A (CsA) and chitosan carboxylate (CC) to be used as an implantable subcutaneous medical device for a prolonged therapeutic effect in the treatment of...
View PublicationCausal relationship between humidifier disinfectant exposure and Th17-mediated airway inflammation and hyperresponsiveness
-
Song MK, Kim DI, Lee K ,
-
Toxicology ,
-
2021,
-
454:152739
- Instrument: NucleoCounter® NC-250™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Diesel exhaust particulates (DEP) have adverse effects on the respiratory system. Endoplasmic reticulum (ER) abnormalities contribute to lung inflammation. However, the relationship between DEP exposure and ER stress in the respiratory immune system and especially the alveolar macrophages (AM) is...
View PublicationAn Exploratory Study of the Relationships Between Diesel Engine Exhaust Particle Inhalation, Pulmonary Inflammation and Anxious Behavior
-
Jeong S, Lee JH, Ha JH, et al. ,
-
International Journal of Environmental Research and Public Health ,
-
2021,
-
18(3):1166
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Genome editing has shown great promise for clinical translation but also revealed the risk of genotoxicity caused by off-target effects of programmable nucleases. Here we describe chromosomal aberrations analysis by single targeted linker-mediated PCR sequencing (CAST-Seq), a preclinical assay to...
View PublicationDiesel Exhaust Particulates Enhances Susceptibility of LPS-Induced Acute Lung Injury through Upregulation of the IL-17 Cytokine-Derived TGF-β1/Collagen I Expression and Activation of NLRP3 Inflammasome Signaling in Mice
-
Kim DI, Song MK, Lee K ,
-
Biomolecules ,
-
2021,
-
11(1):67
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
The potential of adoptive cell therapy can be extended when combined with genome editing. However, variation in the quality of the starting material and the different manufacturing steps are associated with production failure and product contamination. Here, we present an...
View PublicationPhosphorylation of levan by microwave-assisted synthesis enhanced anticancer ability
-
Huang TY, Huang MY, Tsai CK, Su WT ,
-
Journal of Bioscience and Bioengineering ,
-
2021,
-
131(1):98-106
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay, Cell Vitality Assay
- Research area: Oncology
The macrophage-associated molecule CD163 has been reported as a prognostic biomarker in different cancer types, but its role in colorectal cancer (CRC) is unclear. We studied CD163 in the tumor microenvironment and circulation of patients with CRC in relation to...
View PublicationOsmolality Effects on CHO Cell Growth, Cell Volume, Antibody Productivity and Glycosylation
-
Alhuthali S, Kotidis P, Kontoravdi C ,
-
International Journal of Molecular Sciences ,
-
2021,
-
22(7):3290
- Instrument: NucleoCounter® NC-250™
- Cell Type: CHO Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
MCF7 cells acquire estrogen-independent proliferation after long-term estrogen deprivation (LTED), which recapitulates endocrine therapy resistance. LTED cells can become primed for apoptosis, but the underlying mechanism is largely unknown. We previously reported that Eleanor non-coding RNAs (ncRNAs) upregulate the ESR1...
View PublicationAcoustophoresis Enables the Label-Free Separation of Functionally Different Subsets of Cultured Bone Marrow Stromal Cells
-
Olm F, Lim HC, Schallmoser K, et al. ,
-
Cytometry A ,
-
2021,
-
99(5):476-487
- Instrument: NucleoCounter® NC-250™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay, Cell Cycle Assay
- Research area: Stem Cell Therapy
Objective: As the development and progression of colorectal cancer (CRC) are known to be affected by the immune system, cell subsets such as T cells, natural killer (NK) cells, and natural killer T (NKT) cells are considered interesting targets for...
View PublicationTargeting EML4-ALK gene fusion variant 3 in thyroid cancer
-
Aydemirli MD, van Eendenburg JDH, van Wezel T, et al. ,
-
Endocr Relat Cance ,
-
2021,
-
28(6):377-389
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
The WD40-containing E3 ubiquitin ligase RFWD3 has been recently linked to the repair of DNA damage by homologous recombination (HR). Here we have shown that an RFWD3 mutation within the WD40 domain is connected to the genetic disease Fanconi anemia...
View PublicationInhalation exposure by cigarette smoke: Effects on the progression of bleomycin- and lipopolysaccharide-induced lung injuries in rat models
-
Lee SM, Shin YJ, Go RE, et al. ,
-
Toxicology ,
-
2021,
-
451:152695
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Currently available toxicity data on humidifier disinfectants are primarily limited to polyhexamethylene guanidine phosphate-induced lung fibrosis. We, therefore, investigated whether the sterilizer component Kathon, which is a mixture of chloromethylisothiazolinone and methylisothiazolinone, induces fibrotic lung injury following direct lung exposure...
View PublicationPreexisting and post-COVID-19 immune responses to SARS-CoV-2 in cancer patients
-
Bilich T, Roerden M, Maringer Y, et al. ,
-
Cancer Discov ,
-
2021,
-
candisc.0191.2021
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Background: Metformin, a biguanide, is one of the most commonly prescribed treatments for type 2 diabetes and has recently been recommended as a potential drug candidate for advanced cancer therapy. Although Metformin has antiproliferative and proapoptotic effects on breast cancer,...
View PublicationRepeated intratracheal instillation of zinc oxide nanoparticles induced pulmonary damage and a systemic inflammatory response in cynomolgus monkeys
-
Park EJ, Kim SN, Yoon C, et al. ,
-
Nanotoxicology ,
-
2021,
-
Jan/15
- Instrument: NucleoCounter® NC-250™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Pancreatic islets comprise of endocrine cells with distinctive hormone expression patterns. The endocrine cells show functional differences in response to normal and pathological conditions. The goal of this protocol is to generate high-quality, large-scale transcriptome data of each endocrine cell...
View PublicationPreclinical Evaluation of a Novel TALEN Targeting CCR5 Confirms Efficacy and Safety in Conferring Resistance to HIV-1 Infection
-
Romito M, Juillerat A, Kok YL, et al. ,
-
Biotechnol J ,
-
2021,
-
16(1):e2000023
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Background: The thiosemicarbazone CD 02750 (VLX50) was recently reported as a hit compound in a phenotype-based drug screen in primary cultures of patient tumor cells. We synthesized a copper complex of VLX50, denoted VLX60, and characterized its antitumor and mechanistic...
View PublicationQuantitative evaluation of chromosomal rearrangements in gene-edited human stem cells by CAST-Seq
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Turchiano G, Andrieux G, Klermund J, et al. ,
-
Cell Stem Cell ,
-
2021,
-
28(6):1136-1147.e5
- Instrument: NucleoCounter® NC-250™
- Cell Type: HSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The craniofacial disorder mandibulofacial dysostosis Guion-Almeida type is caused by haploinsufficiency of the U5 snRNP gene EFTUD2/SNU114. However, it is unclear how reduced expression of this core pre-mRNA splicing factor leads to craniofacial defects. Here we use a CRISPR-Cas9 nickase...
View PublicationIntegrating Human-Induced Pluripotent Stem Cell Expansion Capability and Cardiomyocyte Differentiation Potential in a Microcarrier Suspension Culture
-
Ho V, Tong G, Lam A, et al. ,
-
Methods Mol Biol ,
-
2021,
- Instrument: NucleoCounter® NC-250™
- Assay: Cell Count & Viability Assay
- Research area: Cardiology
Human-induced pluripotent stem cells are known for their high proliferation capacity as well as their ability to differentiate to different lineages (Ban et al., Theranostics 7(7):2067–2077, 2017; Chen et al., Stem Cell Res 15(2):365–375, 2015; Serra et al., Trends Biotechnol...
View PublicationThe histone methyltransferase Ezh2 restrains macrophage inflammatory responses
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Kitchen GB, Hopwood T, Gali Ramamoorthy T, et al. ,
-
FASEB J ,
-
2021,
-
35(10):e21843
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
Robust inflammatory responses are critical to survival following respiratory infection, with current attention focused on the clinical consequences of the Coronavirus pandemic. Epigenetic factors are increasingly recognized as important determinants of immune responses, and EZH2 is a prominent target due...
View PublicationSoluble PD-1 (sPD-1) is expressed in human macrophages
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Antonsen KW, Hviid CVB, Hagensen MK, et al. ,
-
Cell Immunol ,
-
2021,
-
369:104435
- Instrument: NucleoCounter® NC-250™
- Cell Type: Immune Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell-based Immunotherapy
The PD-1/PD-L1 axis plays a crucial role in regulating the anti-tumour immune response. A soluble PD-1 protein (sPD-1) has previously been observed, which could block the binding of PD-L1 to PD-1. Tumour associated macrophages are abundant in tumours, and evidence...
View PublicationMolecular characteristics and spatial distribution of adult human corneal cell subtypes
-
Ligocki AJ, Fury W, Gutierrez C, et al. ,
-
Sci Rep ,
-
2021,
-
11(1):16323
- Instrument: NucleoCounter® NC-250™
- Cell Type: Dendritic Cells
- Assay: Cell Count & Viability Assay
- Research area: Ophthalmology
Bulk RNA sequencing of a tissue captures the gene expression profile from all cell types combined. Single-cell RNA sequencing identifies discrete cell-signatures based on transcriptomic identities. Six adult human corneas were processed for single-cell RNAseq and 16 cell clusters were...
View PublicationAnti-obesity activity of Heracleum moellendorffii root extracts in 3T3-L1 adipocytes
-
Geum NG, Son HJ, Yeo JH, et al. ,
-
Food Sci Nutr ,
-
2021,
-
9(11):5939-5945
- Instrument: NucleoCounter® NC-250™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
It has been reported that H. mollendorffii roots (HMR) have various pharmacological activities such as anti-inflammatory activity and immunostimulatory activity. However, the anti-obesity activity of HMR has not been studied. Thus, we evaluated in vitro anti-obesity of HMR in mouse...
View PublicationLabel-free separation of neuroblastoma patient-derived xenograft (PDX) cells from hematopoietic progenitor cell products by acoustophoresis
-
Olm F, Panse L, Dykes JH, et al. ,
-
Stem Cell Res Ther ,
-
2021,
-
12(1):542
- Instrument: NucleoCounter® NC-250™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Stem Cell Therapy
The impact of the glycan distribution on the in vivo function and half-life of monoclonal antibodies has long motivated the genetic engineering of producer cells to achieve structures that enhance efficacy, safety and stability. To facilitate glycoengineering of IgG-producing Chinese...
View PublicationRelationship between Somatic Cell Count and Different Microbial and Chemical Quality Parameters of Bulk Tank Milk
-
El-Shaimaa MN, El-Shafaie MA, Fahim KM, Sallam SS ,
-
Advances in Animal and Veterinary Sciences ,
-
2021,
-
9(10): 1660-1668
- Instrument: NucleoCounter® SCC-100™
- Cell Type: Mammalian Cells
- Research area: Dairy (SCC)
The objective of this study was to investigate bulk tank somatic cell count and associated risk factors influencing the hygienic quality and chemical composition of raw milk; additionally, to assess the impact of somatic cell count (SCC/ml) on the microbial...
View PublicationCrude coriander oil in the diet of lactating goats enhanced lactational performance, ruminal fermentation, apparent nutrient digestibility, and blood chemistry
-
Kholif AE, Elazab MA, Matloup OH, et al. ,
-
Small Ruminant Research ,
-
2021,
-
204(106522):0921-4488
- Instrument: NucleoCounter® SCC-100™
- Cell Type: Mammalian Cells
- Research area: Dairy (SCC)
The use of safe feed additives in diets of lactating animals has gained increasing interest. The effects of feeding lactating goats on diet supplemented with crude coriander oil at different levels were evaluated. Thirty multiparous lactating Damascus × Baladi goats...
View PublicationEffect of double freezing fish semen on sperm motility and fertility
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J Nynca, S Judycka, E Liszewska, et al. ,
-
Aquaculture ,
-
2021,
-
530:735782
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Freezing cooled-transported semen allows veterinarians and breeders to collect and process the semen of stallions on farm, and then ship the semen to a semen freezing center. There, however, is a lack of standardization of shipping and freezing protocols. The...
View PublicationTowards standardization of the cryopreservation procedure of cultured pikeperch (Sander lucioperca) semen
-
Judycka S, Dietrich MA, Żarski D, et al. ,
-
Aquaculture ,
-
2021,
-
358:736539
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
In modern livestock breeding, cryopreserved semen is routinely used for artificial insemination. Sperm cryopreservation allows for long-term storage of insemination doses and secures reproduction at a desired time point. In order to cryopreserve semen, it needs to be carefully processed...
View PublicationThe Effects of Antifreeze Protein III Supplementation on the Cryosurvival of Goat Spermatozoa During Cryopreservation
-
Lv C, Larbi A, Memon S, et al. ,
-
Biopreservation and Biobanking ,
-
2021,
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
It is widely recognized that quality of spermatozoa in sexed semen (SS) samples is not as great as for conventional, non-sexed semen (NS). There are differences in qualitative and biochemical variables between spermatozoa in NS and SS. Information, however, is...
View PublicationThe effects of metabolic substrates glucose, pyruvate, and lactate added to a skim milk-based semen extender for cooled storage of stallion sperm
-
Hernández-Avilés C, Ramírez-Agámez L, Love CC, et al. ,
-
Theriogenology ,
-
2021,
-
161:83-97
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
To improve artificial insemination protocols in ovine species it is crucial to optimize sperm quality evaluation after preservation technologies. Emerging technologies based on novel biomolecules and related to redox balance and proteins involved in sperm motility such as ProAKAP4 could...
View PublicationCryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm
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Oldenhof H, Wolkers WF, Sieme H ,
-
Methods in Molecular Biology ,
-
2021,
-
2180:365-377
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Differences in the cryotolerance of spermatozoa exist among stallions, but it remains to be determined to what extent such differences are affected by breed. In this study, post-thaw semen quality in stallions presented for semen cryopreservation was analysed retrospectively (1012...
View PublicationUsing egg yolk in a TRIS-Equex STM paste extender for freezing of dog semen is superior to egg yolk plasma, also after addition of lecithin and catalase
-
Schäfer-Somi S, Binder C, Burak J, et al. ,
-
Cryobiology ,
-
2021,
-
S0011-2240(21)00069-9
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
We compared the results of using egg yolk plasma (EYP) instead of egg yolk (EY) in a TRIS-based Equex STM Paste freezing extender system for dog semen [25]. We also tested whether the addition of lecithin and catalase to the...
View PublicationSuitability of semen stress tests for predicting fertilizing capacity of boar ejaculates
-
Schulze M, Mohammadpour F, Schröter F, et al. ,
-
Theriogenology ,
-
2021,
-
176:73-81
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Despite the relatively long history of captive breeding, the Arctic charr still exhibits a generally low, but highly variable reproductive performance in aquaculture. A recent publication exposed potential paternal factors influencing the reproductive outcome of Arctic charr broodstock from the...
View PublicationEffects from disruption of mitochondrial electron transport chain function on bull sperm motility
-
Bulkeley EA, Foutouhi A, Wigney K, et al. ,
-
Theriogenology ,
-
2021,
-
176:63-72
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Besides classical semen parameters, semen stress tests (SSTs) are helpful tools to assess the fertilizing capacity of sperm. However, valid studies on SSTs in relation to fertility are rare because several corrections of common fertility parameters for female and male...
View PublicationCommercial application of flow cytometry for evaluating bull sperm
-
DeJarnette JM, Harstine BR, McDonald K, Marshall CE ,
-
Anim Reprod Sci ,
-
2021,
-
3:106838
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Sperm mitochondrial function is essential for normal physiology and fertility, but the importance of mitochondrial activity to support specific sperm functions, such as motility, varies between species. It was previously believed that mitochondrial function was not necessary for bull sperm...
View PublicationDevelopment of a new antimicrobial concept for boar semen preservation based on bacteriocins
-
Jakop U, Hensel B, Orquera S, et al. ,
-
Theriogenology ,
-
2021,
-
173:163-172
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Artificial insemination using semen from genetically superior sires remains one of the most effective biotechnologies ever commercialized for animal breeding purposes. Genetic progress, however, cannot begin until conception occurs. Processing laboratories that provide cryopreserved bull semen for commercial use depend...
View PublicationCool-stored and frozen-thawed stallion semen: thoughts on collection, evaluation, processing, insemination, and fertility
-
Hernández-Avilés C, Love, C ,
-
Clinical Theriogenology ,
-
2021,
-
0,736111111
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
The conventional storage temperature of 16-18 °C provides optimal conditions for the preservation of boar sperm quality, which are extremely cold sensitive cells. On the other hand, however, it requires the addition of antibiotics to inhibit bacterial growth. Rising numbers...
View PublicationA Modified Flotation Density Gradient Centrifugation Technique Improves the Semen Quality of Stallions with a High DNA Fragmentation Index
-
Umair M, Henning H, Stout TAE, Claes A ,
-
Animals ,
-
2021,
-
11(7):1973
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Semen evaluation is a fundamental part of breeding soundness examination of males, including stallions. New technologies have been implemented in both the research arena and clinical practice for analysis of stallion sperm; therefore, theriogenologists and general equine practitioners should become...
View PublicationProteomic analysis of pikeperch seminal plasma provides novel insight into the testicular development of domesticated fish stocks
-
Dietrich MA, Judycka S, Żarski D, et al. ,
-
Animal ,
-
2021,
-
15(7):100279
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Sperm DNA fragmentation compromises fertilization and early embryo development. Since spermatozoa lack the machinery to repair DNA damage, to improve the likelihood of establishing a healthy pregnancy, it is preferable to process ejaculates of stallions with a high sperm DNA...
View PublicationEffect of glucose concentration and cryopreservation on mitochondrial functions of bull spermatozoa and relationship with sire conception rate
-
Moraes CR, Moraes LE, Blawut B, et al. ,
-
Anim Reprod Sci ,
-
2021,
-
230:106779
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Control of the reproduction of domesticated stocks is considered a prerequisite for aquaculture development of pikeperch. However, knowledge about the physiology of the captive pikeperch male reproductive system and the biology of semen is very limited, especially regarding protein characteristics....
View PublicationFlow-cytometric analysis of membrane integrity of stallion sperm in the face of agglutination: the “zombie sperm” dilemma
-
Ortiz I, Felix M, Resende H, et al. ,
-
J Assist Reprod Genet ,
-
2021,
-
38(9):2465-2480
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Mitochondrial function is essential for sperm viability, not only from a sperm metabolism perspective, but also for improvement of sperm storage in liquid and frozen states. Bull sperm have notable metabolic variability with energy production for motility and subsequently for...
View PublicationPSVI-9 Effects of Mixing Prior to Automated Packaging in Extended Boar Semen
-
Suarez-Trujillo A, Minton A, Nicholls G, et al. ,
-
Journal of Animal Science ,
-
2021,
-
99:221-222
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Purpose: To define the effect of sperm agglutination, associated with incubation under capacitating conditions, on accuracy of membrane assessment via flow cytometry and to develop methods to mitigate that effect. Methods: Sperm motility was measured by CASA. Sperm were stained...
View PublicationIntrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares
-
Segabinazzi LGTM, Canisso IF, Podico G, et al. ,
-
Antibiotics (Basel) ,
-
2021,
-
10(5):490
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
It is known that sperm cells will settle out of extender over time which could impact the concentration per dose within a batch. The objective of this study to investigate the effects of mixing prior to packaging on concentration of...
View PublicationAssessment of an iPad-based sperm motility analyzer for determination of canine sperm motility
-
Bulkeley E, Collins C, Foutouhi A, et al. ,
-
Transl Anim Sci ,
-
2021,
-
5(2):txab066
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Microorganisms, including pathogenic or opportunistic bacteria and fungi, may gain access to the uterus during breeding, and infectious endometritis plays a major role in equine subfertility. This study aimed to assess the post-breeding inflammatory response, endometrial culture, and embryo recovery...
View PublicationCoenzyme Q-10 improves preservation of mitochondrial functionality and actin structure of cryopreserved stallion sperm
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Lançoni R, Celeghini ECC, Giuli V, et al. ,
-
Anim Reprod ,
-
2021,
-
18(1):e20200218
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
The objective of this study was to evaluate the repeatability and accuracy of canine sperm motility (total and progressive) assessment with a tablet-based Canine iSperm instrument compared to computer-assisted sperm analysis (CASA). The experiment used fresh and frozen/thawed canine semen...
View PublicationCentrifugal force assessment in ram sperm: identifying species-specific impact
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Neila-Montero M, Riesco MF, Alvarez M, et al. ,
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Acta Vet Scand ,
-
2021,
-
63(1):42
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Coenzyme Q-10 (CoQ-10) is a cofactor for mitochondrial electron transport chain and may be an alternative to improve sperm quality of cryopreserved equine semen. This work aimed to improve stallion semen quality after freezing by adding CoQ-10 to the cryopreservation...
View PublicationImpact of antibiotics on spermatozoa quality and bacterial load of chilled-stored camels (Camelus dromedarius) semen
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Ghoneim IM, Al-Mubarak AH, Fayez MM, et al. ,
-
Trop Anim Health Prod ,
-
2021,
-
54(1):21
- Instrument: NucleoCounter® SP-100™
- Cell Type: Sperm Cells
- Research area: Animal Reproduction
Background: Centrifugation is routinely employed in handling the ejaculates of some species, but it is not part of the commonly used protocols in ram. However, the development and implementation of new assisted reproductive technologies, alternative preservation models based on washing...
View PublicationUnique brewing-relevant properties of a strain of Saccharomyces 2 jurei isolated from ash (Fraxinus excelsior)
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Hutzler M, Michel M, Kunz O, et al. ,
-
bioRxiv ,
-
2021,
- Instrument: NucleoCounter® YC-100™
- Cell Type: Yeast Cells
- Research area: Brewing
The successful application of Saccharomyces eubayanus and Saccharomyces paradoxus in brewery fermentations has highlighted the potential of wild yeast for brewing, and prompted investigation into the application potential of other members of the genus. Here, we evaluate, for the first...
View PublicationFrozen-dough baking potential of psychrotolerant Saccharomyces species and derived hybrids
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Magalhães F, Calton A, Heiniö RL, Gibson B ,
-
Food Microbiology ,
-
2021,
-
94:103640
- Instrument: NucleoCounter® YC-100™
- Cell Type: Yeast Cells
- Research area: Cell Biology
Despite Saccharomyces cerevisiae being a synonym for baker’s yeast, the species does not perform well in all baking-related conditions. In particular, dough fermentation, or proofing, is compromised by the species’ sensitivity to the low and freezing temperatures that are often...
View Publication2020
The calcium-sensing receptor is essential for calcium and bicarbonate sensitivity in human spermatozoa
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Boisen IM, Rehfeld A, Mos I, et al. ,
-
The Journal of Clinical Endocrinology and Metabolism ,
-
2020,
-
dgaa937
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Sperm Cells
- Assay: FlexiCyte™ Assay
- Research area: Animal Reproduction
Context: The calcium-sensing receptor (CaSR) is essential to maintain a stable calcium concentration in serum. Spermatozoa are exposed to immense changes in concentrations of CaSR ligands such as calcium, magnesium, and spermine during epididymal maturation, in the ejaculate, and in...
View PublicationDoxorubicin generates senescent microglia that exhibit altered proteomes, higher levels of cytokine secretion, and a decreased ability to internalize amyloid β
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Marques L, Johnson AA, Stolzing A ,
-
Experimental Cell Research ,
-
2020,
-
395(2):112203
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Yeast Cells
- Assay: Cell Count & Viability Assay
- Research area: Brewing
Cellular senescence is defined by irreversible cell-cycle arrest and is an evolutionarily conserved hallmark of aging. In this study, we generate senescent microglial cells via exposure to the chemotherapy drug doxorubicin. Compared to control cells, doxorubicin-treated microglia exhibited an altered...
View PublicationCharacterization of Human Mitochondrial PDSS and COQ Proteins and Their Roles in Maintaining Coenzyme Q 10 Levels and Each Other’s Stability
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Hsiu-Chuan Yen , Wen-Yu Yeh , Szu-Hsien Lee , et al. ,
-
Biochim Biophysica Acta Bioenergetics ,
-
2020,
-
1861(7):148192
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Mutations of many PDSS and COQ genes are associated with primary coenzyme Q10 (CoQ10) deficiency, whereas mitochondrial DNA (mtDNA) mutations might cause secondary CoQ10 deficiency. Previously, we found that COQ5 and COQ9 proteins are present in different protein complexes in...
View PublicationAir Quality Intervention During the Nanjing Youth Olympic Games Altered PM Sources, Chemical Composition, and Toxicological Responses
-
Teemu J Rönkkö , Maija-Riitta Hirvonen , Mikko S Happo , et al. ,
-
Environmental Research ,
-
2020,
-
185:109360
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Ambient particulate matter (PM) is a leading global environmental health risk. Current air quality regulations are based on airborne mass concentration. However, PM from different sources have distinct chemical compositions and varied toxicity. Connections between emission control measures, air quality,...
View PublicationNear-Random Distribution of Chromosome-Derived Circular DNA in the Condensed Genome of Pigeons and the Larger, More Repeat-Rich Human Genome
-
Henrik Devitt Møller , Jazmín Ramos-Madrigal , Iñigo Prada-Luengo , et al. ,
-
Genome Biology and Evolution ,
-
2020,
-
12(1):3762-3777
- Instrument: NucleoCounter® NC-3000™
- Cell Type: PBMCs
- Research area: Cell Biology
Extrachromosomal circular DNA (eccDNA) elements of chromosomal origin are known to be common in a number of eukaryotic species. However, it remains to be addressed whether genomic features such as genome size, the load of repetitive elements within a genome,...
View PublicationDevelopment of a 3D Tissue-Engineered Skeletal Muscle and Bone Co-culture System
-
Nicholas M Wragg , Diogo Mosqueira , Lia Blokpeol-Ferreras , et al. ,
-
Biotechnology Journal ,
-
2020,
-
15(1):e1900106
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Research area: Cell Biology
In vitro 3D tissue-engineered (TE) structures have been shown to better represent in vivo tissue morphology and biochemical pathways than monolayer culture, and are less ethically questionable than animal models. However, to create systems with even greater relevance, multiple integrated...
View PublicationTranscription Factor 4 Regulates the Regeneration of Corneal Endothelial Cells
-
Jin Sun Hwang , Chang Ki Yoon , Joon Young Hyon , et al. ,
-
Investigatie Ophthalmology & Visual Science ,
-
2020,
-
61(4):21
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Research area: Cell Biology
Purpose: Human corneal endothelial cells (hCECs) have limited regenerative capacity in vivo. Reduced hCEC density results in bullous keratopathy requiring corneal transplantation. This study reveals the role of transcription factor 4 (TCF4) in hCEC diseases and suggests that TCF4 may...
View PublicationStereoisomer-specific ginsenoside 20(S)-Rg3 reverses replicative senescence of human diploid fibroblasts via Akt-mTOR-Sirtuin signaling.
-
Yang KE, Jang HJ,, et al. ,
-
Journal of ginseng research ,
-
2020,
-
44(2):341-349
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Cycle Assay
- Research area: Cell Biology
Background: The replicative senescence of human dermal fibroblasts (HDFs) is accompanied by growth arrest. In our previous study, the treatment of senescent HDFs with Rg3(S) lowered the intrinsic reactive oxygen species (ROS) levels and reversed cellular senescence by inducing peroxiredoxin-3,...
View PublicationIncreased antioxidant response in medium-chain acyl-CoA dehydrogenase deficiency: does lipoic acid have a protective role?
-
Nochi Z, Birkler RID, Fernandez-Guerra P, et al. ,
-
Pediatric Research ,
-
2020,
-
88(4):556-564
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Mitochondrial Potential Assay
- Research area: Cell Biology
Background: Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency (MCADD) is the most frequent fatty acid oxidation (FAO) defect in humans. MCAD-deficient fibroblasts are more resistant to oxidative stress-induced cell death than other FAO defects and healthy controls. Methods: Herein we investigate the...
View PublicationSingle-cell Transcriptomics From Human Pancreatic Islets: Sample Preparation Matters
-
Lori L Bonnycastle , Derek E Gildea , Tingfen Yan , et al. ,
-
Biology Methods and Protocols ,
-
2020,
-
5(1):bpz019
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Single-cell RNA sequencing (scRNA-seq) of human primary tissues is a rapidly emerging tool for investigating human health and disease at the molecular level. However, optimal processing of solid tissues presents a number of technical and logistical challenges, especially for tissues...
View PublicationToxicological Evaluation of Exhaust Emissions From Light-Duty Vehicles Using Different Fuel Alternatives in Sub-Freezing Conditions
-
Henri Hakkarainen , Päivi Aakko-Saksa , Maija Sainio , et al. ,
-
Particle and Fibre Toxicology ,
-
2020,
-
17(1):17
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Eukaryotic Cells
- Assay: Cell Count & Viability Assay, Cell Vitality Assay
- Research area: Cell Biology
Background: Emissions from road traffic are under constant discussion since they pose a major threat to human health despite the increasingly strict emission targets and regulations. Although the new passenger car regulations have been very effective in reducing the particulate...
View PublicationMutations in a Single Signaling Pathway Allow Cell Growth in Heavy Water
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Caroline Kampmeyer , Jens V Johansen , Christian Holmberg , et al. ,
-
ACS synthetic biology ,
-
2020,
-
9(4):733-748
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Yeast Cells
- Research area: Cell Biology
Life is completely dependent on water. To analyze the role of water as a solvent in biology, we replaced water with heavy water (D2O) and investigated the biological effects by a wide range of techniques, using Schizosaccharomyces pombe as model...
View PublicationMulti-walled Carbon Nanotubes (MWCNTs) Transformed THP-1 Macrophages Into Foam Cells: Impact of Pulmonary Surfactant Component Dipalmitoylphosphatidylcholine
-
Jinru Lin , Ying Jiang , Yingmei Luo , et al. ,
-
Journal of Hazardous Materials ,
-
2020,
-
392:122286
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Vitality Assay
- Research area: Cell Biology
Pulmonary surfactant or its components can function as barriers toward nanomaterials (NMs) entering pulmonary systems. However, since pulmonary surfactant mainly consists of lipids, it may be necessary to investigate the effects of co-exposure to NMs and pulmonary surfactant or its...
View PublicationOptimized High-Contrast Brightfield Microscopy Application for Noninvasive Proliferation Assays of Human Cell Cultures
-
Carlsen J, Cömert C, Bross P, Palmfeldt J ,
-
ASSAY and Drug Development Technologies ,
-
2020,
-
18(5):215-225
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
High-contrast brightfield (HCBF) microscopy has emerged as a strong tool for noninvasive counting of cells in culture. HCBF imaging delivers precise cell growth data and is completely label free rendering it an attractive alternative to common cell counting procedures that...
View PublicationAnthocyanins decrease the internalization of TiO2 nanoparticles into 3D Caco-2 spheroids
-
Wang J, Zhang J, Li S, et al. ,
-
Food Chemistry ,
-
2020,
-
331:127360
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Epithelial Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
The influence of food components on nanoparticle (NP) internalization indicates a need to investigate the behaviors of NPs in a complex system. This study measured the changes of TiO2 NP colloidal stability and quenching of anthocyanin fluorescence to indicate NP-anthocyanin...
View PublicationLong non-coding RNA LASSIE regulates shear stress sensing and endothelial barrier function
-
Stanicek L, Lozano-Vidal N, Bink DI, et al. ,
-
Communications Biology ,
-
2020,
-
3(1):265
- Instrument: NucleoCounter® NC-3000™
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Blood vessels are constantly exposed to shear stress, a biomechanical force generated by blood flow. Normal shear stress sensing and barrier function are crucial for vascular homeostasis and are controlled by adherens junctions (AJs). Here we show that AJs are...
View PublicationDevelopment of a non-viral platform for rapid virus-like particle production in Sf9 cells
-
Puente-Massaguer E, Gòdia F, Lecina M ,
-
Journal of Biotechnology ,
-
2020,
-
322:43-53
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Insect Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Insect cells have shown a high versatility to produce multiple recombinant products. The ease of culture, low contamination risk with human pathogens and high expression capacity makes an attractive platform to generate virus-like particles (VLPs). The baculovirus expression vector system...
View PublicationQuantification of the HIV‐1 virus‐like particle production process by super‐resolution imaging: From VLP budding to nanoparticle analysis
-
González-Domínguez I, Puente-Massaguer E, Cervera L, Gòdia F ,
-
Biotechnology and Bioengineering ,
-
2020,
-
117(7):1929-1945
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Insect Cells
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Virus-like particles (VLPs) offer great promise in the field of nanomedicine. Enveloped VLPs are a class of these nanoparticles and their production process occurs by a budding process, which is known to be the most critical step at intracellular level....
View PublicationHuman early mesenchymal stromal cells delivered on porous lightweight biodegradable polycaprolactone-based microcarriers result in improved cartilage formation
-
Lin YM, Lam ATL, Lee J, et al. ,
-
Materialia ,
-
2020,
-
13:100851
- Instrument: NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay
- Research area: Cell Biology
Porous lightweight polycaprolactone (LPCL)-based microcarriers (MC) can be used for large-scale production of human mesenchymal stromal cells (MSC) in stirred bioreactors. These biodegradable MC can serve in delivering MSC, which exhibit improved bone healing in a rat calvarial defect model....
View PublicationBisphenol A Diglycidyl Ether (BADGE) and Bisphenol Analogs, but Not Bisphenol A (BPA), Activate the CatSper Ca2+ Channel in Human Sperm
-
Rehfeld A, Andersson AM, Skakkebæk NE ,
-
Frontiers in Endocrinology ,
-
2020,
-
11:324
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Sperm Cells
- Assay: FlexiCyte™ Assay
- Research area: Cell Biology
Aim: Evidence suggests that bisphenol A diglycidyl ether (BADGE), bisphenol A (BPA), and BPA analogs can interfere with human male fertility. However, the effect directly on human sperm function is not known. The CatSper Ca2+ channel in human sperm controls...
View PublicationDistributed Automated Manufacturing of Pluripotent Stem Cell Products
-
Maryam Shariatzadeh , Amit Chandra , Samantha L Wilson , et al. ,
-
the international journal of advanced manufacturing technology ,
-
2020,
-
106(3):1085-1103
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Research area: Cell-based Immunotherapy
Establishing how to effectively manufacture cell therapies is an industry-level problem. Decentralised manufacturing is of increasing importance, and its challenges are recognised by healthcare regulators with deviations and comparability issues receiving specific attention from them. This paper is the first...
View PublicationMultiplexed Quantitative Proteomic Analysis of HEK293 Provides Insights Into Molecular Changes Associated With the Cell Density Effect, Transient Transfection, and Virus-Like Particle Production
-
Jesús Lavado-García , Inmaculada Jorge , Laura Cervera , et al. ,
-
Journal of Proteome Research ,
-
2020,
-
19(3):1085-1099
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Gene Therapy
The production of virus-like particles (VLPs) has gained importance over the last few years owing to the benefits they provide compared to conventional vaccines. The biopharmaceutical industry is currently searching for safer candidates based on VLPs for new and existing...
View PublicationQuantification of the HIV-1 Virus-Like Particle Production Process by Super-Resolution Imaging: From VLP Budding to Nanoparticle Analysis
-
Irene González-Domínguez , Eduard Puente-Massaguer , Laura Cervera , Francesc Gòdia ,
-
Biotechnology and Bioengineering ,
-
2020,
-
117(7):1929-1945
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Insect Cells
- Assay: Cell Count & Viability Assay
- Research area: Gene Therapy
Virus-like particles (VLPs) offer great promise in the field of nanomedicine. Enveloped VLPs are a class of these nanoparticles and their production process occurs by a budding process, which is known to be the most critical step at intracellular level....
View PublicationDouble Controlled Release of Therapeutic RNA Modules through Injectable DNA-RNA Hybrid Hydrogel
-
Han S, Park Y, Kim H, et al. ,
-
ACS Applied Materials & Interfaces ,
-
2020,
-
12(50):55554-55563
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Gene Therapy
Advances in the DNA nanotechnology have enabled the fabrication of DNA-based hydrogels with precisely controlled structures and tunable mechanical and biological properties. Compared to DNA hydrogel, preparation of RNA-based hydrogel remains challenging due to the inherent instability of naked RNA....
View PublicationA facile and scalable in production non-viral gene engineered mesenchymal stem cells for effective suppression of temozolomide-resistant (TMZR) glioblastoma growth
-
Tu GXE, Ho YK, Ng ZX, et al. ,
-
Stem Cell Research & Therapy ,
-
2020,
-
11(1):391
- Instrument: NucleoCounter® NC-3000™
- Cell Type: MSCs
- Assay: Cell Count & Viability Assay, GFP Transfection Efficiency Assay
- Research area: Gene Therapy
Background: Mesenchymal stem cells (MSCs) serve as an attractive vehicle for cell-directed enzyme prodrug therapy (CDEPT) due to their unique tumour-nesting ability. Such approach holds high therapeutic potential for treating solid tumours including glioblastoma multiforme (GBM), a devastating disease with...
View PublicationIntegrating Nanoparticle Quantification and Statistical Design of Experiments for Efficient HIV-1 Virus-Like Particle Production in High Five Cells
-
Eduard Puente-Massaguer , Martí Lecina , Francesc Gòdia ,
-
Applied Microbiology and Biotechnology ,
-
2020,
-
104(4):1569-1582
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Immunology
The nature of enveloped virus-like particles (VLPs) has triggered high interest in their application to different research fields, including vaccine development. The baculovirus expression vector system (BEVS) has been used as an efficient platform for obtaining large amounts of these...
View PublicationImmune System Development Varies According to Age, Location, and Anemia in African Children
-
Danika L Hill , Edward J Carr , Tobias Rutishauser , et al. ,
-
Science Translational Medicine ,
-
2020,
-
12(529):eaaw9522
- Instrument: NucleoCounter® NC-3000™
- Cell Type: PBMCs
- Research area: Immunology
Children from low- and middle-income countries, where there is a high incidence of infectious disease, have the greatest need for the protection afforded by vaccination, but vaccines often show reduced efficacy in these populations. An improved understanding of how age,...
View PublicationGraphene Oxide Size-Dependently Altered Lipid Profiles in THP-1 Macrophages
-
Yingmei Luo , Jinfeng Peng , Chaobo Huang , Yi Cao ,
-
Ecotoxicology and environmental safety ,
-
2020,
-
199:110714
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Leukocytes
- Research area: Immunology
Previous studies focused on biocompatibility of graphene oxide (GO) to macrophages, but the impact of GO on lipid profiles in macrophages was less investigated. Herein, we investigated the interactions between THP-1 macrophages and GO of different sizes (GO of size...
View PublicationMebendazole is unique among tubulin-active drugs in activating the MEK–ERK pathway
-
Andersson CR, Selvin T, Blom K, et al. ,
-
Scientific Reports ,
-
2020,
-
10(1):13124
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Cycle Assay
- Research area: Immunology
We recently showed that the anti-helminthic compound mebendazole (MBZ) has immunomodulating activity in monocyte/macrophage models and induces ERK signalling. In the present study we investigated whether MBZ induced ERK activation is shared by other tubulin binding agents (TBAs) and if...
View PublicationInduction of lipid droplets in THP-1 macrophages by multi-walled carbon nanotubes in a diameter-dependent manner: A transcriptomic study
-
Yang T, Chen J, Gao L, et al. ,
-
Toxicology Letters ,
-
2020,
-
332:65-73
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Count & Viability Assay
- Research area: Immunology
Exposure to multi-walled carbon nanotubes (MWCNTs) might induce lipid droplet (LD) biogenesis, but the roles of physicochemical properties of MWCNTs, as well as the mechanisms, remain poorly understood. In this study, we investigated lipid laden foam formation in THP-1 macrophages...
View PublicationEnhancement of Autophagy and Solubilization of Ataxin-2 Alleviate Apoptosis in Spinocerebellar Ataxia Type 2 Patient Cells
-
Jonathan Henry Wardman , Emil Elbæk Henriksen , Adele Gabriele Marthaler , et al. ,
-
Cerebellum ,
-
2020,
-
19(2):165-181
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Cell Vitality Assay
- Research area: Neurobiology
Spinocerebellar ataxia type 2 (SCA2), a rare polyglutamine neurodegenerative disorder caused by a CAG repeat expansion in the ataxin-2 gene, exhibits common cellular phenotypes with other neurodegenerative disorders, including oxidative stress and mitochondrial dysfunction. Here, we show that SCA2 patient...
View PublicationMitochondrial Function in Gilles De La Tourette Syndrome Patients With and Without Intragenic IMMP2L Deletions
-
Victoria A Bjerregaard , Bitten Schönewolf-Greulich , Lene Juel Rasmussen , et al. ,
-
Frontiers in Neurology ,
-
2020,
-
0,571527778
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Mammalian Cells
- Assay: Annexin V Assay
- Research area: Neurobiology
Background: Gilles de la Tourette syndrome (GTS) is a neurodevelopmental condition characterized by motor and vocal tics. The underlying etiology remains largely unknown, and GTS is considered as a complex multifactorial disorder associated with effects of several genes in combination...
View PublicationTranscriptomic analysis links diverse hypothalamic cell types to fibroblast growth factor 1-induced sustained diabetes remission
-
Bentsen MA, Rausch DM, Mirzadeh Z, et al. ,
-
Nature Communications ,
-
2020,
-
11(1):4458
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Neural Cells
- Assay: Cell Count & Viability Assay
- Research area: Neurobiology
In rodent models of type 2 diabetes (T2D), sustained remission of hyperglycemia can be induced by a single intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1), and the mediobasal hypothalamus (MBH) was recently implicated as the brain area responsible...
View PublicationA Synthetic Pan-Aurora Kinase Inhibitor, 5-Methoxy-2-(2-methoxynaphthalen-1-yl)-4H-chromen-4-one, Triggers Reactive Oxygen Species-Mediated Apoptosis in HCT116 Colon Cancer Cells
-
Lee JY, Ahn SS, Jeong YJ, et al. ,
-
Journal of Chemistry ,
-
2020,
-
3025281
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells
- Assay: Cell Count & Viability Assay
- Research area: Oncology
Aurora kinases are Ser/Thr kinases that function as mitotic regulators. 5-Methoxy-2-(2-methoxynaphthalen-1-yl)-4H-chromen-4-one (DK1913) is a synthetic pan-Aurora kinase inhibitor. However, the mode of action of DK1913 concerning the induction of apoptosis is unclear. Here, we report that DK1913 triggered apoptosis, as...
View PublicationOvercoming Multidrug Resistance by Activating Unfolded Protein Response of the Endoplasmic Reticulum in Cisplatin-Resistant A2780/CisR Ovarian Cancer Cells
-
Euitaek Jung , Dongsoo Koh , Yoongho Lim , et al. ,
-
BMB Reports ,
-
2020,
-
53(2):88-93
- Instrument: NucleoCounter® NC-3000™
- Cell Type: Cancer Cells