Solution 2

Propidium Iodide Staining Reagent

Product number: 910-3002
Size: 25 ml

Storage: 2-7°C

Solution 2 – Propidium Iodide (PI) stains nucleic acid of fixed and permeabilized cells. The solution contains PI (20 µg/ml ∼ 30 µM ∼ 0.002%) and Triton X-100 (1 mg/ml ∼ 1.6 mM ∼ 0.1%) in PBS (pH 7.4).

Solution 2 – PI can be used to quantify DNA content of e.g. animal cells and yeast cells, and is a competent dye for measurement of G0/G1, S and G2/M cell cycle phases. The fluorescence intensity of PI stained cell is in stoichiometric relationship to the DNA content. For accurate DNA content measurements using Solution 2 – PI, cells must be fixed/permeabilized and RNase treated prior to staining. Please refer to the specific Application Notes for detailed instructions.

The solution is for research use only (RUO) and is not for diagnostic or therapeutic uses. Please refer to the documents below for further details. Order the product in our Webshop.

Use for

Fixed Cell Cycle – PI Assay with the NucleoCounter® NC-250™ and the NucleoCounter® NC-3000™.

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