Solution 3

DAPI Staining Reagent

Product number: 910-3003
Size: 25 ml

Storage: 2-7°C

Solution 3 – DAPI stains nucleic acid of fixed and permeabilized cells. The solution contains 4′,6-diamidino-2-phenylindole (DAPI; 1 µg/ml ∼ 3 µM ∼ 0.0001%) and Triton X-100 (1 mg/ml ∼ 1.6 mM ∼ 0.1%) in PBS (pH 7.4).

Solution 3 – DAPI can be used to quantify DNA content of e.g. animal cells, allowing for determination of G0/G1, S and G2/M cell cycle phases. The intensity of fluorescence intensity of DAPI stained cell is in stoichiometric relationship to the DNA content. For accurate DNA content measurements using Solution 3 – DAPI, cells must be fixed/permeabilized and RNase treated prior to staining. Please refer to the specific Application Notes for detailed instructions.

The solution is for research use only (RUO) and is not for diagnostic or therapeutic uses. Please refer to the documents below for further details. Order the product in our Webshop.

Use for

Fixed Cell Cycle Assay with the NucleoCounter® NC-250™ and the NucleoCounter® NC-3000™.
DNA Fragmentation Assay with the NucleoCounter® NC-3000™.

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