Solution 5

VB-48™•PI•AO Staining Reagent

Product number: 910-3005
Size: 1 ml

Storage: < 5°C. Protect against moisture and light

Solution 5 – VB-48•PI•AO is a staining reagent containing a thiol-reactive fluorescent probe, VitaBright-48, propidium iodide (PI) and acridine orange (AO). Solution 5 – VB-48•PI•AO enables the user to detect changes in cell vitality.

Solution 5 – VB-48•PI•AO employs three cell staining dyes: AO stains all nucleated cells, PI stains nonviable cells only, and VB-48™ stains cells in an intensity-dependent manner reflecting their redox status. VitaBright-48 can be used as a marker for detection of midstage to late apoptosis in mammalian cells as high levels of reduced thiols within any given cell will result in a high fluorescence intensity of VB-48™. Please refer to the specific Application Notes for detailed instructions.

The solution is for research use only (RUO) and is not for diagnostic or therapeutic uses. Please refer to the documents below for further details. Order the product in our Webshop.

Use for

Vitality Assay with the NucleoCounter® NC-3000™.

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