Can the NucleoCounter® count heavily aggregated cells?

Absolutely.NucleoCounter® instruments offer robust and reliable solutions for counting cells in aggregates and clumps. They use fluorescent dyes to stain cell nuclei and employ a powerful algorithm to segment single nuclei within each smaller aggregate.

For more heavily clumped samples, a numerical estimation of the level of cell aggregation enables the operator to make an informed decision on whether to switch to the dedicated Aggregated Cell Assay, which disassembles cells with a lysis step.