Eliminate human bias in cell counting
Using the Via1-Cassette™ and Via2-Cassette™
Precision, accuracy and reproducibility
See how it works
Calibration Measuring Chamber
NucleoCounter® NC-200™, NC-202™ and NC-3000™ analyze a total volume of 1.4 µL sample (1.35 µl for the NC-202™) stained with the fluorescent dyes acridine orange and DAPI. The large volume increases precision compared to manual counting where the standard is to count 100 cells, regardless of the concentration of cells and number of squares counted, or to count 0.4 µl of volume regardless of the number of cells counted.
Via1-Cassette™ and Via2-Cassette™ are individually volume-calibrated and coded for control of the exact measuring chamber volume. In a standard hemocytometer the spacing can vary up to 10-50%, leading to inaccurate cell concentration calculations.
Via1-Cassette™ and Via2-Cassette™ function as single use pipettes with preloaded dyes, simplifying how you obtain your cell sample, mix cells with dyes and load your sample onto the cell counter, all to eliminate operator bias. Pressing the piston creates a vacuum pulling the cell sample into the cell staining channels without the usual pipetting errors, thereby increasing precision.
Via1-Cassette™ and Via2-Cassette™ mixing channels are preloaded with acridine orange for total cell stain and DAPI for non-viable cell stain. The intricate shape of the mixing channels ensures even mixing of cells and dyes and delivery of a homogeneous sample into the measuring chamber.
A safe, non-toxic cell counting system
Many cell counting methods, whether automated or manual, use trypan blue to stain and identify dead cells. Trypan blue is toxic, and potentially exposes the cell counting operator while pipetting and handling the stain and cell sample. The Via-Cassette is preloaded with acridin orange (AO) and DAPI to stain all cells and dead cells, respectively. Furthermore, the Via-Cassette is a custom-made disposable plastic device designed for optimal sample handling and safe disposal. The sample and stains are contained within the Via-Cassette and will not be released during use. It can be safely disposed of after use, providing a safe working environment for the user.
Please see the Via-Cassette Conformity Statement for further details.
* For low viability samples, Via2-Cassette™ can be used on the NucleoCounter® NC-200™ instrument with a special protocol supplied by ChemoMetec Support