Eliminate human bias in cell counting
Using the Via1-Cassette™
The volume calibrated single use Via1-Cassette™ is a true all-in-one solution for optimized cell count. The cassette is a disposable plastic unit that contains the fluorescent dye used for analysis. A cell sample is easily loaded into the cassette by submerging the inbuilt pipette into the cell suspension and pressing the piston.
Precision, accuracy and reproducibility
The cassettes are loaded with immobilized acridine orange and DAPI for the definition of total cell and dead cell population, respectively. The definition of cells is performed with the software based on acridine orange signal and will be the same in every analysis. No human bias will influence the result. The cassettes have a pre-calibrated volume of the measurement chamber giving a precise and reproducible result. No user or pipetting errors interfere with data generation, and the instrument will tell you if you have too few or too many cells in the sample.
See how it works
Calibration Measuring Chamber
NucleoCounter® NC-200™ and NC-3000™ analyzes a total volume of 1.4 µL sample stained with the fluorescent dyes Acridine Orange and DAPI. The large volume increases precision compared to manual count where the standard is to count 100 cells, regardless of the concentration of cells and number of squares counted. Or to count 0.4 µl of volume regardless of cells counted.
All Via1-Cassettes™ are calibrated and coded in order to maintain control of the exact volume loaded into the measuring chamber unlike in a standard hemocytometer where after filling, the spacing can vary up to 10-50%.
The Via1-Cassette™ is functionally a single use pipette with preloaded dyes making it simple to obtain cell samples from your suspension, simplifying the loading procedure and eliminating operator bias. Pressing this piston creates a vacuum that pulls the cell sample into the cell staining channels without the normal pipetting errors, and this way increasing precision.
The Via1-Cassette™ mixing channels are preloaded with Acridine Orange for total stain and DAPI for non-viable stain. The advanced shape of the mixing channels ensures a good even mixing with the dyes and delivery of a homogeneous sample into the measuring chamber.
(Hover a mouse pointer over the gray areas on the cassette)